Protein Alignment Best4 and Best1

DIOPT Version :10

• Sequence 1: NP_001097609.1
• Gene: Best4 / 39660
• FlyBaseID: FBgn0036491
• Length: 526
• Species: Drosophila melanogaster
• Sequence 2: NP_036043.2
• Gene: Best1 / 24115
• MGIID: 1346332
• Length: 551
• Species: Mus musculus
• Alignment Length: 564
• Identity: 198/564 - (35%)
• Similarity: 293/564 - (51%)
• Gaps: 105/564 - (18%)

  Fly     1 MTVTYTSRVSTSSVLSNFLGLLVRWRGSIYKLIWLDLIVFFGLYCLLAITYRLLVNEEGKRFFEA 65
            ||:|||::|:.:. |.:|..||:.||||||||::.:.:||..||..:...||::::.:.:..||.
Mouse     1 MTITYTNKVANAR-LGSFSSLLLCWRGSIYKLLYGEFLVFIFLYYSIRGLYRMVLSSDQQLLFEK 64

  Fly    66 IMSYCEKNGSLIPLSFVLGFFVRIVMNRWWDQYTTIPWPDGIAILISTSIHGSDDRARVMRRTIL 130
            :..||:....|||:||||||:|.:|::|||.||..:||||.:.|.:|:.:.|.|:..|::|||::
Mouse    65 LALYCDSYIQLIPISFVLGFYVTLVVSRWWSQYENLPWPDRLMIQVSSFVEGKDEEGRLLRRTLI 129

  Fly   131 RYVCLCQVIVFTMISPRVKRRFPTYTQIIEAGFLLENEKKIIEAMDQAFPSYPKHWMPIVWAASI 195
            ||..|.||::...||..|.:||||...::.|||:...|.|.::.:.....::   |:|.||.|::
Mouse   130 RYAILGQVLILRSISTSVYKRFPTLHHLVLAGFMTHGEHKQLQKLGLPHNTF---WVPWVWFANL 191

  Fly   196 VMRARRENKIRDDYAVKTIIDELNQLRGNCGFLLYYDWISVPLVYTQVVTVATYSFFLFSVLGQQ 260
            .|:|....:|||...::::::|:..||..||.|..|||||:|||||||||||.|||||..::|:|
Mouse   192 SMKAYLGGRIRDTVLLQSLMNEVCTLRTQCGQLYAYDWISIPLVYTQVVTVAVYSFFLACLIGRQ 256

  Fly   261 WNESHSDEDGTRIRRWFPILTVLQFFFYMGWLKVAETLINPFGEDDDDFELNWIIDRNLTVSYCI 325
            :...:.|..|..:....|:.|:|||.||||||||||.|||||||||||||.||||||||.||...
Mouse   257 FLNPNKDYPGHEMDLVVPVFTILQFLFYMGWLKVAEQLINPFGEDDDDFETNWIIDRNLQVSLLS 321

  Fly   326 VDEMHQEHPELVKDQYWEEVFPNEIPY--AQPKMRQNPPAAST-----------AYMESQSKLQK 377
            ||.|||..|.:.:|.||.|..| :.||  |..:.|::....||           .:.:.::...|
Mouse   322 VDGMHQNLPPMERDMYWNEAAP-QPPYTAASARSRRHSFMGSTFNISLKKEDLELWSKEEADTDK 385

  Fly   378 RHESAMSTLSTRSRNNFRQNFLG---------------------RPSATGSYSDSIQMEDGPSRV 421
            :.....||:..         |||                     .|...|...|:.|
Mouse   386 KESGYSSTIGC---------FLGLQPKNYHLPLKDLKTKLLCSKNPLLEGQCKDANQ-------- 433

  Fly   422 TFSENQFDL-------------------PH--DEANSSHKPSQS-----------TIDFNDLIEQ 454
               :||.|:                   .|  .:|.|||...||           :.|:.::...
Mouse   434 ---KNQKDVWKFKGLDFLKCVPRFKRRGSHCGPQAPSSHPTEQSAPSSSDTGDGPSTDYQEICHM 495

  Fly   455 RRR----------ERRERIRHRFLDMQSTH----LRHDTSGAPY 484
            :::          ...|.::.|.||..||:    ::......||
Mouse   496 KKKTVEFNLNIPESPTEHLQQRRLDQMSTNIQALMKEHAESYPY 539

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Best4	NP_001097609.1	Bestrophin	8..317	CDD:460047	141/308 (46%)
Best1	NP_036043.2	Bestrophin	8..313	CDD:460047	141/308 (46%)
		Auto-inhibitory segment. /evidence=ECO:0000250|UniProtKB:O76090	346..379		6/32 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	459..489		7/29 (24%)