Protein Alignment Best4 and Best1

DIOPT Version :8

• Sequence 1: NP_001097609.1
• Gene: Best4 / 39660
• FlyBaseID: FBgn0036491
• Length: 526
• Species: Drosophila melanogaster
• Sequence 2: NP_036043.2
• Gene: Best1 / 24115
• MGIID: 1346332
• Length: 551
• Species: Mus musculus
• Alignment Length: 564
• Identity: 198/565 (35%)
• Similarity: 293/565 (52%)
• Gaps: 105/565 (19%)

  Fly     1 MTVTYTSRVSTSSVLSNFLGLLVRWRGSIYKLIWLDLIVFFGLYCLLAITYRLLVNEEGKRFFEA 65
            ||:|||::|:.:. |.:|..||:.||||||||::.:.:||..||..:...||::::.:.:..||.
Mouse     1 MTITYTNKVANAR-LGSFSSLLLCWRGSIYKLLYGEFLVFIFLYYSIRGLYRMVLSSDQQLLFEK 64

  Fly    66 IMSYCEKNGSLIPLSFVLGFFVRIVMNRWWDQYTTIPWPDGIAILISTSIHGSDDRARVMRRTIL 130
            :..||:....|||:||||||:|.:|::|||.||..:||||.:.|.:|:.:.|.|:..|::|||::
Mouse    65 LALYCDSYIQLIPISFVLGFYVTLVVSRWWSQYENLPWPDRLMIQVSSFVEGKDEEGRLLRRTLI 129

  Fly   131 RYVCLCQVIVFTMISPRVKRRFPTYTQIIEAGFLLENEKKIIEAMDQAFPSYPKHWMPIVWAASI 195
            ||..|.||::...||..|.:||||...::.|||:...|.|.::.:.....::   |:|.||.|::
Mouse   130 RYAILGQVLILRSISTSVYKRFPTLHHLVLAGFMTHGEHKQLQKLGLPHNTF---WVPWVWFANL 191

  Fly   196 VMRARRENKIRDDYAVKTIIDELNQLRGNCGFLLYYDWISVPLVYTQVVTVATYSFFLFSVLGQQ 260
            .|:|....:|||...::::::|:..||..||.|..|||||:|||||||||||.|||||..::|:|
Mouse   192 SMKAYLGGRIRDTVLLQSLMNEVCTLRTQCGQLYAYDWISIPLVYTQVVTVAVYSFFLACLIGRQ 256

  Fly   261 WNESHSDEDGTRIRRWFPILTVLQFFFYMGWLKVAETLINPFGEDDDDFELNWIIDRNLTVSYCI 325
            :...:.|..|..:....|:.|:|||.||||||||||.|||||||||||||.||||||||.||...
Mouse   257 FLNPNKDYPGHEMDLVVPVFTILQFLFYMGWLKVAEQLINPFGEDDDDFETNWIIDRNLQVSLLS 321

  Fly   326 VDEMHQEHPELVKDQYWEEVFPNEIPY--AQPKMRQNPPAAST-----------AYMESQSKLQK 377
            ||.|||..|.:.:|.||.|..| :.||  |..:.|::....||           .:.:.::...|
Mouse   322 VDGMHQNLPPMERDMYWNEAAP-QPPYTAASARSRRHSFMGSTFNISLKKEDLELWSKEEADTDK 385

  Fly   378 RHESAMSTLSTRSRNNFRQNFLG---------------------RPSATGSYSDSIQMEDGPSRV 421
            :.....||:..         |||                     .|...|...|:.|
Mouse   386 KESGYSSTIGC---------FLGLQPKNYHLPLKDLKTKLLCSKNPLLEGQCKDANQ-------- 433

  Fly   422 TFSENQFDL-------------------PH--DEANSSHKPSQS-----------TIDFNDLIEQ 454
               :||.|:                   .|  .:|.|||...||           :.|:.::...
Mouse   434 ---KNQKDVWKFKGLDFLKCVPRFKRRGSHCGPQAPSSHPTEQSAPSSSDTGDGPSTDYQEICHM 495

  Fly   455 RRR----------ERRERIRHRFLDMQSTH----LRHDTSGAPY 484
            :::          ...|.::.|.||..||:    ::......||
Mouse   496 KKKTVEFNLNIPESPTEHLQQRRLDQMSTNIQALMKEHAESYPY 539

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Best4	NP_001097609.1	Bestrophin	8..319	CDD:279411	144/311 (46%)
Best1	NP_036043.2	Bestrophin	8..315	CDD:279411	144/311 (46%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930				C62975732
eggNOG	1	0.900				E1_KOG3547
Hieranoid	1	1.000
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	396	1.000	Inparanoid score	I1942
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010
OrthoDB	1	1.010				D145964at33208
OrthoFinder	1	1.000				FOG0000272
OrthoInspector	1	1.000				T130816
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100			O	PTHR10736
Phylome	1	0.910		0.889	Consistency score
RoundUp	0	0.000	Not matched by this tool.
TreeFam	1	0.960
	11	10.870