Protein Alignment sti and gek

DIOPT Version :10

• Sequence 1: NP_001261768.1
• Gene: sti / 39429
• FlyBaseID: FBgn0002466
• Length: 1858
• Species: Drosophila melanogaster
• Sequence 2: NP_523837.2
• Gene: gek / 37858
• FlyBaseID: FBgn0023081
• Length: 1637
• Species: Drosophila melanogaster
• Alignment Length: 2004
• Identity: 449/2004 - (22%)
• Similarity: 750/2004 - (37%)
• Gaps: 713/2004 - (35%)

  Fly    66 LLDAFCLLYNECDKDTLKKRDRNIAEFVNKFRPIIEETRKLRVNADDFLIKTLIGQGYFGNVHLV 130
            |||.|.:||:||...:| :|::.:::|:...:|.:...||||::.|||.|..:||:|.||.|.:|
  Fly    54 LLDTFIVLYDECSNSSL-RREKGVSDFLKLSKPFVHIVRKLRLSRDDFDILKIIGRGAFGEVCVV 117

  Fly   131 VERQTNDIYAMKKIKK----SVVTTSQVKEERDIMSIRNSEWLINLQYAFQDNDNLYLVMEYMPG 191
            ....|..:||||.:.|    ....|:..:||||::...:.:|:.||.||||||.||||||:|..|
  Fly   118 QMISTEKVYAMKILNKWEMLKRAETACFREERDVLVFGDRQWITNLHYAFQDNINLYLVMDYYCG 182

  Fly   192 GDLLSLMSR-HGPFDEDLARFYLAELTVALHTLHEMGYVHRDIKPENILIDRFGHIKLADFGNAA 255
            ||||:|:|: .....||:|:||:.|:.:|::::|::.||||||||:|:|:|:.||::|||||:..
  Fly   183 GDLLTLLSKFEDKLPEDMAKFYITEMILAINSIHQIRYVHRDIKPDNVLLDKRGHVRLADFGSCL 247

  Fly   256 ALDRDGHVLSLSPVGTPDYIAPELLQTISTYKLSKSMHDVSRIVSCDYWSMGIIGYELICETTPF 320
            .||:||.|.|...|||||||:||:|:.:..   .|..:.    ..||:||:|:..||::...|||
  Fly   248 RLDKDGTVQSNVAVGTPDYISPEILRAMED---GKGRYG----TECDWWSLGVCMYEMLYGETPF 305

  Fly   321 HEDNVHETYSKILSH--CEESHLKELISFPADLKVSVNYRNLIESLVTNPSKRLSYERIK---NH 380
            :.:::.|||.||::|  |.....:|.:::    |||...::|:..|:..|..||....|:   :|
  Fly   306 YAESLVETYGKIMNHQNCFNLPSQETLNY----KVSETSQDLLCKLICIPENRLGQNGIQDFMDH 366

  Fly   381 PFFSEIPWGSIRSQVPPIIPTVRSDDDTSNF---EDGIRHKTRREQGVAKKSLTTNMKSN---DF 439
            |:|..|.|.:||....|.:|.|.|..|||||   ::.:|             ||.::..:   .|
  Fly   367 PWFVGIDWKNIRQGPAPYVPEVSSPTDTSNFDVDDNDVR-------------LTDSIPPSANPAF 418

  Fly   440 SGKDLPFIGYSFVHMEKSAISATTDEKLQEKLKELLQKLKTRENEISMLKQDLLRAQQSLRKTDN 504
            ||..|||||::|     |..|::|              |.:::|:.|....|.|       .|.:
  Fly   419 SGFHLPFIGFTF-----SLTSSST--------------LDSKKNQSSGFGDDTL-------DTIS 457

  Fly   505 KSQVVADAKMEIKKLQQIIKEKTMELTTCKTQIKTLQSSAKIDEEMWSKKEATITDLLRLNRQKY 569
            ..|:.            |:.....|......|:|.|..
  Fly   458 SPQLA------------ILPSNNSETPVDSVQLKALND--------------------------- 483

  Fly   570 EEAKIASEQRYEKQLADKKQELASTLQKLDARELEFNAKFEECKHLSMKLQNYKDMLQQIKEQNL 634
                         |||..|||.|. |.|      :.|..||..|....:||:      .|.::|:
  Fly   484 -------------QLAALKQEKAE-LSK------QHNEVFERLKTQDSELQD------AISQRNI 522

  Fly   635 KSETNHEEQRRQMAELYEQKLTDLRKKVRDSQDTNRRMTMEIKEIRTELDESISSSKSTQEAKNA 699
             :...:.|...:::||..|| ..|.::|||.::              |||.::..:.|.:.....
  Fly   523 -AMMEYSEVTEKLSELRNQK-QKLSRQVRDKEE--------------ELDGAMQKNDSLRNELRK 571

  Fly   700 TERNIEEILRRLNEEIASNNELHAEKVKLETKLQLKENETQEVRAE--CHRLERELQLAECRCQL 762
            :::...|:            |||.|...:|.   .||.:.:| .||  |.:|:.||:......:.
  Fly   572 SDKTRREL------------ELHIEDAVIEA---AKEKKLRE-HAEDCCRQLQMELRKGSSSVET 620

  Fly   763 A-ESSLATQVSPYETAPGSLTELNAIEDQLRADLLAAKESENHQKGRADQLQTLVTKLEQMLERF 826
            . ..|:::::|.|        |:..:|       |...|..:||:.|.:.      :||.:.|:|
  Fly   621 TMPLSISSEMSSY--------EIERLE-------LQFSEKLSHQQTRHNM------ELEALREQF 664

  Fly   827 NEQSLSPTKSHSSRKQEGETVGDMLERQNEKLEDKLAAVREQMIVERQAARTANLSLWKVEKQLE 891
            :|                             ||:                  |||:|.|
  Fly   665 SE-----------------------------LEN------------------ANLALTK------ 676

  Fly   892 EALSEKKLLARRMELTEDRIK--KVQNASDEAQRMLKTSQEETRQRESRIEELKQELAAAKRDVL 954
                       .::.|::|:|  ::::.:|.|:.:|:..::...::.|..|| ||.|::
  Fly   677 -----------ELQQTQERLKYTQMESITDSAETLLELKKQHDLEKSSWFEE-KQRLSS------ 723

  Fly   955 KEHRQWEKAEQERMKCKSEIIEHLANVHRLEQQETELRQKLRQIQSRFDGVTLEQKNTIRELQEE 1019
                      :..:|.||                      |:::|:..|.:       .:||:.:
  Fly   724 ----------EVNLKSKS----------------------LKELQAEDDEI-------FKELRMK 749

  Fly  1020 REKSRKANDSCLVLQKELKQLTDNFQRLKYACSITDSQLTEVETMLKSEQERNKSQKSQLDTLHE 1084
            ||                            |.::.:.|:.|:...:..|    |..:..|..|..
  Fly   750 RE----------------------------AITLWERQMAEIIQWVSDE----KDARGYLQALAT 782

  Fly  1085 KLRERNDQLTDLRKQLTTVES----EKRLAEQRAQVLASEIDELR-LNLKEQQKKLVAQQDQLVE 1144
            |:.|..:.|    |.:.|..:    .|....:|:|    ::|::. |||:...::.:..::.:.:
  Fly   783 KMTEELEYL----KHVGTFNNNGVDNKNWRNRRSQ----KLDKMELLNLQSALQREIQAKNMISD 839

  Fly  1145 QTNALFATQERAELLDGQNANYEAQTADSNREMVSLKEENARILSELFHKKEEVGNLQAEIRGLE 1209
            :     .:|.|::|:..|            :|:...|:....||.:...|:.|:.:||.  .|||
  Fly   840 E-----LSQTRSDLISTQ------------KEVRDYKKRYDSILHDFQKKETELRDLQK--GGLE 885

  Fly  1210 SAQANL----HAEIDSLQDTLAEKEQFYVQRDIKSNATLAQHKKLIDYLQLKVEDLSAKKKKTLA 1270
            .:::.|    |..:.|         .|:  ||:..|:      ::||         ||:.
  Fly   886 YSESFLNKSTHHGLSS---------AFF--RDMSKNS------EIID---------SAES----- 919

  Fly  1271 DKLFGSSHTNKENVSPNDVESSILYRALKEELKREQKMNSLLKEQLAQLNGTATLRSPRKSAAVN 1335
               ||:.  :.:|.:||..:|.                ||          |......|:.:...|
  Fly   920 ---FGNE--SGDNFTPNFFQSG----------------NS----------GMLFNYEPKYAGKNN 953

  Fly  1336 GDSDAPKQRPVSIAALPRSPQKQQQPLKRTTSQVELKTTAEKPTKVTIENQAHHRFELALQESKV 1400
            .|..:.|:..||..:...|.|..::..|:...                 |.|.|:|.:....|..
  Fly   954 KDHSSMKEASVSDLSREESDQLVKESQKKVPG-----------------NTAIHQFLVRTFSSPT 1001

  Fly  1401 DAVNCV-----VCEKAVVAGSPFWKCKECKDVTHRKCRSNVQSHC----GSTK------PT---- 1446
            ...:|.     :..:.||       |:.|....|..|...|.:.|    ..||      ||
  Fly  1002 KCNHCTSLMVGLTRQGVV-------CEICGFACHTICCQKVPTTCPVPMDQTKRPLGIDPTRGIG 1059

  Fly  1447 --------APS-------------------------ADDLSSIQSVS-SLTLDSVD--------- 1468
                    .|.                         :.|..::.||| |..||..|
  Fly  1060 TAYEGYVKVPKSGVIKRGWIRQFVVVCDFKLFLYDISPDRCALPSVSVSQVLDMRDPEFSVGSVR 1124

  Fly  1469 -----------------VAGGTTSGGEYIGSLVYSSDGAEDQ-------------ARKEIEVNCA 1503
                             :......||..:.:|:.:.:.:|..             .|..:.....
  Fly  1125 ESDVIHAAKKDVPCIFKIKTALIDGGLSLNTLMLADNESEKSKWVIALGELHRILKRNSLPNTAI 1189

  Fly  1504 FEVAE--------------------QQILLLGCNTGLYAYHLDSQRLVHISGLESVSCMSICKRL 1548
            |:|.|                    .|| |||...||:..:||...:..|.  ||...:.:....
  Fly  1190 FKVNEILDNTLSLIRNALCSVIIYPNQI-LLGTEDGLFYINLDQYEIARIG--ESKKILQLWYIE 1251

  Fly  1549 AKAIMVGTVGEKLYQCDYRQLESRCQSSSSCHKPVLETSAIELPFANRTPSEKWKLVLISDEAEN 1613
            .:.|:|...|:   |.:.|.|..|          .||.|.:|           |..|:   |::|
  Fly  1252 EEQILVILCGK---QRNLRLLPIR----------ALEASDVE-----------WIKVV---ESKN 1289

  Fly  1614 ALDSVAIAATSTRIVILKYDLKLHMFKPVRALDTATPVTSIFFTRHSAIVSSDKFYEIDLD---- 1674
            .:.  |......|...:.|...:.:.:|               ..|:.||    .|||:..
  Fly  1290 CIS--ACTGIIRRFPNIVYSFIIALKRP---------------NNHTQIV----VYEINRTRTRH 1333

  Fly  1675 --------NYAAEEFVDLSDKSM---------------ESTAK---------CQPLT-----AVR 1702
                    .|.|:....|||..:               |:||.         |..|.     |||
  Fly  1334 QKTCEFTIGYMAQHLQILSDMRLVVAHQSGFTAYFLRGEATAMSLVHPENQLCAFLNYSGVDAVR 1398

  Fly  1703 I---------SRQEYLLCFAEYGVFVDEFGCRSRPYDLNWVYAPTGFVYRDPFLFISHYQSVQIV 1758
            :         :..||||.|....::||..|.:||..::.:...||...:.|..|.:.....:.|.
  Fly  1399 VIEILCPSGGNFGEYLLVFQTLAIYVDLQGRKSRDREIMYPAFPTYITFCDGHLLVFSDTHLDIF 1463

  Fly  1759 RLHRSFSKEMASGDNASENSESPE-LQRVYLPHYMSTLLANSGDVNLYAVAIDQRPCNGTQKIYH 1822
                              |:::.| :|.:.|.  .|..|.|.|:|.|.:|       |.|..|.:
  Fly  1464 ------------------NTQTAEWVQSIGLK--QSLPLNNLGNVVLSSV-------NDTPLIVY 1501

  Fly  1823 LDT----------------MQAFKQKFNIS--METLSSVATSVTLGSTVTGNSI 1858
            |..                :.:.|::|:|.  .:|:.|...|..:.:....|.|
  Fly  1502 LSNIHTKGLLQYRDGNRKGLPSIKRRFSIREINKTIKSDRRSKMISAPTNFNHI 1555

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
sti	NP_001261768.1	Protein Kinases, catalytic domain	111..452	CDD:473864	142/356 (40%)
		SMC_prok_B	481..1254	CDD:274008	143/786 (18%)
		C1	1389..1440	CDD:410341	11/55 (20%)
		CNH	1509..1779	CDD:459938	66/319 (21%)
gek	NP_523837.2	STKc_DMPK_like	98..430	CDD:270748	142/355 (40%)
		KELK	477..556	CDD:464876	30/147 (20%)
		CCDC158	<481..>894	CDD:464943	125/687 (18%)
		C1_MRCK	990..1042	CDD:410359	12/58 (21%)
		PH_MRCK	1049..1182	CDD:269949	15/132 (11%)
		CNH	1215..1484	CDD:459938	71/339 (21%)
		CRIB	1544..1580	CDD:238077	2/12 (17%)