Protein Alignment RasGAP1 and Rasa1

DIOPT Version :10

• Sequence 1: NP_001261664.1
• Gene: RasGAP1 / 39158
• FlyBaseID: FBgn0004390
• Length: 1181
• Species: Drosophila melanogaster
• Sequence 2: NP_663427.2
• Gene: Rasa1 / 218397
• MGIID: 97860
• Length: 1038
• Species: Mus musculus
• Alignment Length: 777
• Identity: 165/777 - (21%)
• Similarity: 301/777 - (38%)
• Gaps: 181/777 - (23%)

  Fly    35 DTREVRIEEQLKVKIGEAKNLSSRNAANTSCS--------TQGTRDVY--CTIALDQEEICRTPT 89
            |..|.:|....|:...||.||..  .....||        |.|...:|  ....:.:.:||.||
Mouse   335 DPHEGKIWFHGKISKQEAYNLLM--TVGQVCSFLVRPSDNTPGDYSLYFRTNENIQRFKICPTP- 396

  Fly    90 IERTLTPFFGEEHQFKIPRRFRYLTIYLWDRDMKQDKPIGKIA--IKREELHMYNHKDHWFSLRP 152
                       .:||.:..|:.              ..||.|.  .::|::     .:.::...|
Mouse   397 -----------NNQFMMGGRYY--------------NSIGDIIDHYRKEQI-----VEGYYLKEP 431

  Fly   153 VD-QDSEVQGMVNVEVAFTEAQQTQSLSEGIDLGQ---HTLRH------HQNLPHHSH------- 200
            |. ||                 |.|.|::.:| |:   :|:|.      ::|:....:
Mouse   432 VPMQD-----------------QGQVLNDTVD-GKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKG 478

  Fly   201 QQRAHLNDYKENSELSNIQRASAAAASSSSAAMTLKTRAA-----GLFGHVHHPPSQTQHFP--- 257
            ::..:|....|.|:...|...|...|:.....:.|...:.     .|||..:......|||.
Mouse   479 KRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEH 543

  Fly   258 -IINTTSTSSDQLSNW-KSHGRFVGVTIKVPACVDLAKKQGTCDPFVVCTAH------------- 307
             |......:.:|..:| |....|..:....|...:...:|.:.....:..||
Mouse   544 YIFYFAGETPEQAEDWMKGLQAFCSLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCN 608

  Fly   308 -YSNKHQVTRRTKQRKKTVDPEFDEAMYFDLHIDADAGSTNTTGSNKSAGSLESSANKGYSIYPV 371
             |.|..||.:  ...::..:|.:.|...|| .:..|......|.|||:..|.:
Mouse   609 IYLNSVQVAK--THAREGQNPVWSEEFVFD-DLPPDINRFEITLSNKTKKSKD------------ 658

  Fly   372 GGADLVEIVVSVWHDAHGAMSDKVFLGEVRLPMLNKQEQQAVNPSAWYYLQPRSMTHSSRSLNAT 436
              .|::.:...:.....|..:|:.||....:|:      :.:.|
Mouse   659 --PDILFMRCQLSRLQKGHATDEWFLLSSHIPL------KGIEP--------------------- 694

  Fly   437 PRSCATPPGTRLSVDSTIGSLRLNLNYTADHVFPLATYDDLMNLLLESVDQRPITVSAVSILGEL 501
                              ||||:...|:.:.:.|...|.:...|:|:.  :..:..:...:.|:
Mouse   695 ------------------GSLRVRARYSMEKIMPEEEYSEFKELILQK--ELHVVYALSHVCGQ- 738

  Fly   502 VSGKTEVAQPLVRLFTHTERIAPIIKALADHEISHLTDPTTIFRGNTLVSKMMDEAMRLSGLHYL 566
              .:|.:|..|:::|.|.:..:.::..|.|.|||...:.||:||..||.|.:|::.|:.:...::
Mouse   739 --DRTLLASILLKIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQYMKATATQFV 801

  Fly   567 HQTLRPVLSQIVAEKKPCEIDPSKIKDRSAVDTNLHNLQDYVERVFEAITKSADRCPKVLCQIFH 631
            |..|:..:.:|:..|:.||:.|||::....|:|||.:|...:..:.|.|..:::..|..|..|:.
Mouse   802 HHALKDSILKIMESKQSCELSPSKLEKNEDVNTNLAHLLSILSELVEKIFMASEILPPTLRYIYG 866

  Fly   632 DLRECAGEHFPSNREVRYSVVSGFIFLRFFAPAILGPKLFDLTTERLDAQTSRTLTLISKTIQSL 696
            .|::.....:|:|..:|..|||||:|||...||||.|::|::.::......:|||||::|::|:|
Mouse   867 CLQKSVQHKWPTNNTMRTRVVSGFVFLRLICPAILNPRMFNIISDSPSPIAARTLTLVAKSVQNL 931

  Fly   697 GNLVSSRSSQQTCKEEFTVELYKKFCTEQH-----VDAVKHFLEVISTPSHASSSVHPAAAA 753
            .|||     :...||.:...:.....:.:|     :|.:.:..|:..|..|:.:.:....||
Mouse   932 ANLV-----EFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRTDLSRDLAA 988

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RasGAP1	NP_001261664.1	C2A_RasA2_RasA3	44..169	CDD:176046	25/137 (18%)
		C2B_RasA3	280..462	CDD:175977	29/195 (15%)
		RasGAP_GAP1_like	473..740	CDD:213330	76/271 (28%)
		PH_GAP1-like	763..867	CDD:269950
		BTK	862..897	CDD:128417
Rasa1	NP_663427.2	SH2_Nterm_RasGAP	152..255	CDD:198216
		SH3_RasGAP	272..330	CDD:212722
		SH2_Cterm_RasGAP	341..417	CDD:198217	21/103 (20%)
		PH_RASA1	480..567	CDD:270080	17/86 (20%)
		C2_Ras_p21A1	582..707	CDD:176045	29/186 (16%)
		RasGAP_p120GAP	705..1036	CDD:213340	81/294 (28%)