Protein Alignment NaCP60E and CACNA1F

DIOPT Version :10

• Sequence 1: NP_001261172.1
• Gene: NaCP60E / 37981
• FlyBaseID: FBgn0085434
• Length: 2896
• Species: Drosophila melanogaster
• Sequence 2: NP_005174.2
• Gene: CACNA1F / 778
• HGNCID: 1393
• Length: 1977
• Species: Homo sapiens
• Alignment Length: 2940
• Identity: 574/2940 - (19%)
• Similarity: 959/2940 - (32%)
• Gaps: 1236/2940 - (42%)

  Fly   104 KSLFLFYPWSPARRVCVYIATNQFFDYCVMATILFNCIFLAM------------TETVEEAEYIF 156
            ::||.....:|.||.|:.|...:.||..::.||..||:.|.:            ...:|:.||:|
Human    70 RALFCLTLANPLRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVF 134

  Fly   157 LAIYSIEMVIKIIAKGFLLNKYTYLRNPWNWLDFVVITSGYATIGMEVG---------------- 205
            |.|:::|.|:||:|.|.:|:...|:||.||.|||:::..|..::.:|.|
Human   135 LVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGG 199

  Fly   206 -NLAGLRTFRVLRALKTVSIMPGLKTIINALLHSFRQLAEVMTLTIFCLMVFALFALQVYMGELR 269
             ::..||.|||||.|:.||.:|.|..::|:::.:...|..:..|.:|.::::|:..|::::|.:.
Human   200 FDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMH 264

  Fly   270 NKCVRQVPTDWTNVSHTDWQIWVNDTDNWLYDEDELPVLCGNLTGARHCPFEYVCLCVGE--NPN 332
            ..|                  :...:|   .:.:|.|..|.:....|.|..... .|.|.  .||
Human   265 KTC------------------YFLGSD---MEAEEDPSPCASSGSGRACTLNQT-ECRGRWPGPN 307

  Fly   333 HGYTNFDNFMWSMLTTFQLITLDYWENV-YNMVLATCGPMSVSFFTVVVFFGSFYLINLMLAVVA 396
            .|.||||||.::|||.||.:|::.|.:| |.|..|....:...:|..:|.||||:::||:|
Human   308 GGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVL---- 368

  Fly   397 LSYEEEAEITNEERKKDLLDHRDDSTFSFDPSVLNVKKLNKNNKKKIDSRKGVLLASYSKKKTRR 461
                                                               |||...:||::.:.
Human   369 ---------------------------------------------------GVLSGEFSKEREKA 382

  Fly   462 KKTKGGKEGGTNGNGNGSNGDDNKSHSATPSPGPSPRHSATERPSALTMQAQKQYQQMEQQHK-- 524
            |                :.||                           .|.|::.||||:..:
Human   383 K----------------ARGD---------------------------FQKQREKQQMEEDLRGY 404

  Fly   525 ---LAKSGSGGSNNPMAPTPKGRISFQDSGMGVKNPNML-YPSDYKGQL--IANSGQPSSNSSGV 583
               :.::......:|.|....|  |..:.|.....|.:. ..:..:|:|  .::|.: |::|:..
Human   405 LDWITQAEELDMEDPSADDNLG--SMAEEGRAGHRPQLAELTNRRRGRLRWFSHSTR-STHSTSS 466

  Fly   584 NRESSQDDSGVV-----DDHEEQDTTNDMGHVSTVELALS----PREVRLIKCNGNIARIKNHNV 639
            :......|:|.:     |:.||:      |.:::....|:    .|..|.::....:.|.:
Human   467 HASLPASDTGSMTETQGDEDEEE------GALASCTRCLNKIMKTRVCRRLRRANRVLRAR---- 521

  Fly   640 YALHQEFSSEVVVIDDLPDRNCDRCVHWCTDYESWLQFQNCLYKVVRDPLFELAITLCIVLNTAF 704
                                 |.|.|.           .|..|         .|:.|.:.|||..
Human   522 ---------------------CRRAVK-----------SNACY---------WAVLLLVFLNTLT 545

  Fly   705 LAMEHHGMSESFRNALDVGNKVFTSIFTFECIVKLMALSKDFFLCG-WNIFDLLIVTASLLDIIF 768
            :|.||||.........:..|||...:||.|.::||..|....::.. :|.||..:|...:|:.
Human   546 IASEHHGQPVWLTQIQEYANKVLLCLFTVEMLLKLYGLGPSAYVSSFFNRFDCFVVCGGILET-- 608

  Fly   769 ELVD-------GLSVLRGLRLLRVLKLAQSWTTMKVLLSIIISTIGALGNLTLILVIVIYIFAVI 826
            .||:       |:||||.:||||:.|:.:.|.::..|::.:::::.::.:|.|:|.:.|.||:::
Human   609 TLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLL 673

  Fly   827 GMQLFSKDYTPEKFDPDPVPRWNFNDFFHSFMMIFRILCGE-WIEPLWDCMRAEEE---QGASTC 887
            |||||...:   .||.....|..|:.|..:.:.:|:||.|| |...::|.:.|...   .|...|
Human   674 GMQLFGGKF---NFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVC 735

  Fly   888 FAIFLPTLVMGNFMVLNLFLALLLNSFNSEELKSKKEEVGEESKLARSIERVRDLIRKKRQERKD 952
            . .|:...:.||:::||:|||:.:::..|.:..:.|::.||:|.
Human   736 I-YFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGGEKSN--------------------- 778

  Fly   953 RKERKFAEKFQQIVLDAQQAHAQTLSHQAAVGLERGDKPGVLAETKFHRLSYQESMNRPVSGSDF 1017
                   ||
Human   779 -------EK-------------------------------------------------------- 780

  Fly  1018 GFQIPLHDGLHTIVDGLEYDDTGDLPEQIQLQAHPLPPTSDSMPPTYESAMMATTGGSFSSVNGN 1082
                                   |||::
Human   781 -----------------------DLPQE------------------------------------- 785

  Fly  1083 GTCQNLTPFVQAERRLQHQISSGVSTQQYDSREEATYTESIELLGQYNSTDTDPYANDQRSGCGS 1147
              .:.|.|.|:.|
Human   786 --NEGLVPGVEKE---------------------------------------------------- 796

  Fly  1148 FNRGDSLQDNSSRRYGSEEHDEAFLKYQKSLLTRSPSYRKSLDRLSQSSGQSQRSLLKSEEAEMR 1212
                   ::..:||.|::..:|                                    .||.|
Human   797 -------EEEGARREGADMEEE------------------------------------EEEEE-- 816

  Fly  1213 RHSSGQSLNSMSIEQDELLSQQGNLREELLNCDQKELFQFLQEEEELQKGTKLRRISNVMRSRRP 1277
                         |::|                        :||||...|.:|
Human   817 -------------EEEE------------------------EEEEEGAGGVEL------------ 832

  Fly  1278 SSQMGQPENETMVEHSEFDNIIQSFEKELEEIKRSTTSLERKLSNLSEPSPAADEATKAIMEHIA 1342
                                        |:|:
Human   833 ----------------------------LQEV--------------------------------- 836

  Fly  1343 IITGASERSAADEVVLPLNPYDSYDLSSVPRRSQSVSAAAQRQSVKLKRRSLEKQRKIDEDFSIS 1407
                                        ||:
Human   837 ----------------------------VPK---------------------------------- 839

  Fly  1408 NEIRKICDQIHAPFVAMEAMAVAATSASQAQPNQSPFLRRKVDPFTVQFDRFKRLSLIERVEEVP 1472
                                                                      |:|..:|
Human   840 ----------------------------------------------------------EKVVPIP 846

  Fly  1473 EEEKPISTLRIESEKMPRKFLHGPDQLRLDSLSLKSTNSYENLLIQKQKLGMATPPAVPATPPTS 1537
            |...                          ...|..||
Human   847 EGSA--------------------------FFCLSQTN--------------------------- 858

  Fly  1538 LKSSIEPPTLAQISSLKTTPPLAALTEHQQHFHATSIQAAPTPAHTHAHSQAHAHSMAGQRRRME 1602
                                                      |.....|:..|.|
Human   859 ------------------------------------------PLRKGCHTLIHHH---------- 871

  Fly  1603 HPQSTLDKAASFQSARTESHSSGAADASSALALAMAQKTEQSQSTAPDATQKPSAFTRLTEKPWH 1667
                                                                  .||.|
Human   872 ------------------------------------------------------VFTNL------ 876

  Fly  1668 CLVSYVDDLTVGGRRNSQGAYNDPMTFPSYGATKAAKVPDDCFPQKCYDHFYFRCPWFMSCMDTQ 1732
                                                                             
Human   877 ----------------------------------------------------------------- 876

  Fly  1733 SAKHWTRVRTAVLTVVDTPAFEWFVLVLIFASSITLCFEDINLDKNKTLKRVLYWINFSFCLIFV 1797
                                    :||.|..||::|..|| .:..:.....:|.:.:::|..||.
Human   877 ------------------------ILVFIILSSVSLAAED-PIRAHSFRNHILGYFDYAFTSIFT 916

  Fly  1798 VEMILKWLALGF----SKYFTSFWTILDFIIVFVSVFSLLIEENENLKVLRSLRTLRALRPLRAI 1858
            ||::||....|.    ..:..|::.:||.::|.||:.|..|..:. :.|::.||.||.|||||||
Human   917 VEILLKMTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISFGIHSSA-ISVVKILRVLRVLRPLRAI 980

  Fly  1859 SRWQGMRIVVNALMYAIPSIFNVLLVCLVFWLIFSIMGVQFFGGKFFKCVNE--------MGELL 1915
            :|.:|::.||..:..||.:|.|:::|..:...:|:.:|||.|.|||:.|.:|        .|..|
Human   981 NRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYTCTDEAKHTPQECKGSFL 1045

  Fly  1916 --PITEVNDKWDCIEQNYTWINSKITFDHVGMGYLALLQVATFEGWMEVMADAVDARGVDLQPQR 1978
              |..:|:..   :.:...|:||...||:|....:||..|:|||||..::..|:||...|..|
Human  1046 VYPDGDVSRP---LVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAYAEDHGP-- 1105

  Fly  1979 EANLYAY-----IYFVIFIVCGSFFTLNLFIGVIIDNFNML-KKKYEGGVLEMFLTESQK---HY 2034
               :|.|     ::|:::|:..:||.:|:|:|.:|..|... :::|:...|:    ::|:   .|
Human  1106 ---IYNYRVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELD----KNQRQCVEY 1163

  Fly  2035 YTAMKKLGRKKPQKVIKRPINHFLAMFYDLSNSRRFEIAIFVLIFLNMLTMGIEHYDQPHAVFFI 2099
            ....:.|.|..|    |.|..:   ..:...||..||..:|:||.||.:.:.::||:|.....:.
Human  1164 ALKAQPLRRYIP----KNPHQY---RVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYA 1221

  Fly  2100 LEVSNAFFTTVFGLEAIVKIVGLR-YHYFTVPWNVFDFLLVLASIF----------GILMEDIMI 2153
            :::.|..||.:|.:|.::||:..: .||||..||.||.|:|:.||.          |.|.|....
Human  1222 MDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWNTFDALIVVGSIVDIAVTEVNNGGHLGESSED 1286

  Fly  2154 DLPISPTLLRVVRVFRIGRILRLIKAAKGIRKLLFALVVSLPALFNIGALLGLITFIYAILGMSL 2218
            ...||.|..   |:||:.|:::|:...:|||.||:..:.|..||..:..|:.:|.||||::||.:
Human  1287 SSRISITFF---RLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQM 1348

  Fly  2219 FGNVKLQGA--LDDMVNFQTFGRSMQLLFRLMTSAGWNDVLESLMIQPPD-CDP---FIHGHTNG 2277
            ||.|.||..  ::...|||||.:::.||||..|...|.:::  |...|.: |||   |..|. ..
Human  1349 FGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIM--LASLPGNRCDPESDFGPGE-EF 1410

  Fly  2278 NCGHPLLAITYFTSFIIISYMIVINMYIAIILENFNQAHQEEEIGIVEDDLEMFYIRWSKYDPHA 2342
            .||.. .||.||.||.::...::||:::|:|::||:...::..| :....|:.|...||:|||.|
Human  1411 TCGSN-FAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSI-LGPHHLDEFKRIWSEYDPGA 1473

  Fly  2343 TQFIHFSQLSDFIASLDPPLGISK--PNNVA---LVSFNLPISKGNKIHCLDILHALVKHVLGHV 2402
            ...|....:...:..:.||||..|  |:.||   ||:.|:|::....:.....|.|||:..| .:
Human  1474 KGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSL-KI 1537

  Fly  2403 EETDNFKQLQEQMDVKFKKQFPTRKELEIVSSTRIWKRQEK---------------------AAK 2446
            :...|.:|..:::.:..||               ||||.::                     |..
Human  1538 KTEGNLEQANQELRIVIKK---------------IWKRMKQKLLDEVIPPPDEEEVTVGKFYATF 1587

  Fly  2447 TIQTGWKEYLRRKREKERSNSGDSATQTSSPGGWQSKLSALNFFHLQVSRRGTACSSRASSRKSS 2511
            .||..::::.|||.:....|  |:|..|||  ..|:.|.:|.....:: |:...|.:.....:..
Human  1588 LIQDYFRKFRRRKEKGLLGN--DAAPSTSS--ALQAGLRSLQDLGPEM-RQALTCDTEEEEEEGQ 1647

  Fly  2512 RASDASDLSELAGPWLNLPLMLVSGADEVVKDIKQQNDELGKRGS-IFVEAPRASRRRSFYNFFL 2575
            ...:..|..:|.   .|...|:              :....:||| |.|..|...|.....:|..
Human  1648 EGVEEEDEKDLE---TNKATMV--------------SQPSARRGSGISVSLPVGDRLPDSLSFGP 1695

  Fly  2576 RHQDAVDDSLTSPSV-------HRK--TAMNNTTNTTSNSA------------------------ 2607
            ...|....:.:.|||       ||:  .|:..|.....||.
Human  1696 SDDDRGTPTSSQPSVPQAGSNTHRRGSGALIFTIPEEGNSQPKGTKGQNKQDEDEEVPDRLSYLD 1760

  Fly  2608 STSGTASSTATAP----------------------ATGCGPAAT--------SASD----SDRHQ 2638
            ..:||...:...|                      ..|..|:.|        |..|    ...|:
Human  1761 EQAGTPPCSVLLPPHRAQRYMDGHLVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHR 1825

  Fly  2639 AVGGGSAPSRKRASSFIRKKPPLERGLSAQSALRVNKNAFVSEASAPEVIVTRPSPEQQTHPHSL 2703
              |..|.|:|.:.|   ...|| :||....:.|     ..|.|.:|.|..:.|.|...:|.
Human  1826 --GRNSGPNRAQGS---WATPP-QRGRLLYAPL-----LLVEEGAAGEGYLGRSSGPLRTF---- 1875

  Fly  2704 SLRPDNATLVHVLVHRESEEYKEEDESSPSVSGNGNG--------FGVGLDMLSKQPPPQIRITT 2760
                   |.:||          ....|.||....|:.        ...||.:.::.|    |...
Human  1876 -------TCLHV----------PGTHSDPSHGKRGSADSLVEAVLISEGLGLFARDP----RFVA 1919

  Fly  2761 GSVESSMDTCAMPTVQIMVDSPKDPPRGDFSSAPIDDV-GAPIDVNVQGDTSQVFYDYNPEKATD 2824
            .:.:...|.|.:                     .:|:: .|..|:..|| ||.::.|.....:..
Human  1920 LAKQEIADACRL---------------------TLDEMDNAASDLLAQG-TSSLYSDEESILSRF 1962

  Fly  2825 DQGNGQDETAQFESL 2839
            |:.:..||.|...:|
Human  1963 DEEDLGDEMACVHAL 1977

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NaCP60E	NP_001261172.1	Ion_trans	125..371	CDD:459842	79/277 (29%)
		Ion_trans	687..902	CDD:459842	67/226 (30%)
		Ion_trans	1751..2019	CDD:459842	90/287 (31%)
		Na_channel_gate	2009..2063	CDD:240441	10/57 (18%)
		Ion_trans	2067..2320	CDD:459842	94/269 (35%)
		GPHH	2332..2378	CDD:465306	18/50 (36%)
CACNA1F	NP_005174.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..60
		I	79..375		96/372 (26%)
		Ion_trans	91..383	CDD:459842	93/368 (25%)
		Binding to the beta subunit. /evidence=ECO:0000250	395..412		4/16 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	418..441		6/24 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	455..488		6/33 (18%)
		II	515..761		78/296 (26%)
		Ion_trans	532..763	CDD:459842	73/245 (30%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	767..830		26/342 (8%)
		III	858..1140		97/519 (19%)
		Ion_trans	870..1107	CDD:459842	83/405 (20%)
		Dihydropyridine binding. /evidence=ECO:0000250	1060..1150		31/94 (33%)
		IV	1177..1444		94/276 (34%)
		Ion_trans	1189..1452	CDD:459842	94/269 (35%)
		Dihydropyridine binding. /evidence=ECO:0000250	1397..1463		21/68 (31%)
		Phenylalkylamine binding. /evidence=ECO:0000250	1409..1452		14/43 (33%)
		GPHH	1461..1514	CDD:465306	19/52 (37%)
		Ca_chan_IQ	1524..1598	CDD:462591	16/89 (18%)
		CAC1F_C	1619..1976	CDD:465298	77/432 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1637..1754		23/133 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1816..1841		7/29 (24%)