Protein Alignment NaCP60E and scn4aa

DIOPT Version :10

• Sequence 1: NP_001261172.1
• Gene: NaCP60E / 37981
• FlyBaseID: FBgn0085434
• Length: 2896
• Species: Drosophila melanogaster
• Sequence 2: NP_001034914.2
• Gene: scn4aa / 572442
• ZFINID: ZDB-GENE-051201-2
• Length: 1829
• Species: Danio rerio
• Alignment Length: 2678
• Identity: 811/2678 - (30%)
• Similarity: 1148/2678 - (42%)
• Gaps: 947/2678 - (35%)

  Fly     8 FNDEKAVAKHQVVAYTQRSQVKHENRHIQLVREYGFHPRTKAS-VEDGDVLPRKFEPFPEHMYGK 71
            |..|..|...:::      |.|.....::...|   .|:..:| :|.|..||..:...|..:...
Zfish    16 FTPESLVEIERLI------QEKSTREELEGAEE---EPQAPSSDLEAGKCLPMIYGDPPGDLLNT 71

  Fly    72 PLEEIDTFI-YEETFCVVSKRFRKNYIHRFTGTKSLFLFYPWSPARRVCVYIATNQFFDYCVMAT 135
            |||:||.|. .::||.|:||   .|.|.||:...::|...|:|..||..:.|..:..|...:|.|
Zfish    72 PLEDIDPFYKTQKTFIVISK---GNTIFRFSSEPAMFCISPFSIVRRGAIKILIHSLFSMFIMIT 133

  Fly   136 ILFNCIFLAM------TETVEEAEYIFLAIYSIEMVIKIIAKGFLLNKYTYLRNPWNWLDFVVIT 194
            ||.||:|:.|      ::||   ||:|..||:.|..:|::::||.:..:|:||:|||||||:||:
Zfish   134 ILSNCVFMTMSNPPAWSKTV---EYVFTGIYTFEATVKVLSRGFCIGPFTFLRDPWNWLDFMVIS 195

  Fly   195 SGYATIGMEVGNLAGLRTFRVLRALKTVSIMPGLKTIINALLHSFRQLAEVMTLTIFCLMVFALF 259
            ..|.|..:::||::.||||||||||||::::||||||:.||:.|.:::.:||.||||.|.||||.
Zfish   196 MAYVTEFVDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKMIDVMILTIFALAVFALI 260

  Fly   260 ALQVYMGELRNKCVR--------------QVPTDWT-NVSHT-DWQIWVNDTDNWLYDEDEL-PV 307
            .||::||.||.||:|              .:..|.| ||:.| |::.::::.:|..:.|..| .:
Zfish   261 GLQLFMGNLRQKCIRWPILNSTIFDVYNSNMVNDTTLNVTDTFDFKAYISNEENQYFLEGSLDAL 325

  Fly   308 LCGNLTGARHCPFEYVCLCVGENPNHGYTNFDNFMWSMLTTFQLITLDYWENVYNMVLATCGPMS 372
            ||||.:.|..||..|.|:..|.|||:|||::|||.|:.|..|:|:|.|:|||::.:.|...|...
Zfish   326 LCGNSSDAGRCPEGYTCMKAGRNPNYGYTSYDNFGWAFLALFRLMTQDFWENLFQLTLRAAGKTY 390

  Fly   373 VSFFTVVVFFGSFYLINLMLAVVALSYEEEAEITNEERKKDLLDHRDDSTFSFDPSVLNVKKLNK 437
            :.||.||:|.||||||||:|||||::|:|:.|.|..|.:        |....|...:..:|....
Zfish   391 MIFFVVVIFLGSFYLINLILAVVAMAYDEQNEATLAEAR--------DKEEEFQRLLEQLKNQET 447

  Fly   438 NNKKKIDSRKGVLLASYSKKKTRRKKTKGGKEGGTNGNGNGSNGDDNKSHSATPSPGPSPRHSAT 502
            .:|           ||.:.:||:.:        |:|..|:
Zfish   448 GSK-----------ASLASQKTQSR--------GSNRTGS------------------------- 468

  Fly   503 ERPSALTMQAQKQYQQMEQQHKLAKSGSGGSNNPMAPTPKGRISFQDSGMGVKNPNMLYPSDYKG 567
                               .|.||       :..:.....|||.          |.::
Zfish   469 -------------------LHDLA-------DEDVIKDCNGRIV----------PRLI------- 490

  Fly   568 QLIANSGQPSSNSSGVNRESSQDDSGVVDDHEEQDTTNDMGHVSTVELALSPREVRLIKCNGNIA 632
                           |||.||..:....:|.:...:.:.|.::...:|:
Zfish   491 ---------------VNRVSSNKELSAEEDQKSLSSKHSMQYLDQPKLS---------------- 524

  Fly   633 RIKNHNVYALHQEFSSEVVVIDDLPD--RNC--------DRCVHW--CTDYESWLQFQNCLYKVV 685
               .....||    |.....::.|.|  |.|        |..:.|  |.   .|:.|:..::.||
Zfish   525 ---KRTASAL----SVLTATMEGLEDAQRPCPPGWYKFADMFLKWDCCA---PWILFKKWVHFVV 579

  Fly   686 RDPLFELAITLCIVLNTAFLAMEHHGMSESFRNALDVGNKVFTSIFTFECIVKLMALSK-DFFLC 749
            .||..:|.||:||||||.|:||||:.||..|.:.|.|||.|||.|||.|.:.||:|:.. .:|..
Zfish   580 MDPFVDLGITICIVLNTLFMAMEHYPMSPHFEHVLSVGNLVFTGIFTAEMVFKLIAMDPYYYFQV 644

  Fly   750 GWNIFDLLIVTASLLDIIFELVDGLSVLRGLRLLRVLKLAQSWTTMKVLLSIIISTIGALGNLTL 814
            ||||||.:|||.||:::....|.||||||..|||||.|||:||.|:.:|:.||.:::||||||||
Zfish   645 GWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTL 709

  Fly   815 ILVIVIYIFAVIGMQLFSKDYTP--EKFDPD-PVPRWNFNDFFHSFMMIFRILCGEWIEPLWDCM 876
            :|.|:::||||:|||||.|.|..  .|...| .:|||:.|||||||:::||||||||||.:||||
Zfish   710 VLAIIVFIFAVVGMQLFGKSYKDCVCKISEDCELPRWHMNDFFHSFLIVFRILCGEWIETMWDCM 774

  Fly   877 RAEEEQGASTCFAIFLPTLVMGNFMVLNLFLALLLNSFNSEELKSKKEEVGEES-------KLAR 934
               |..|||.|..:|:..:|:||.:||||||||||:||:.:.| |..::.||.:       ::.|
Zfish   775 ---EVAGASMCLIVFMMVMVIGNLVVLNLFLALLLSSFSGDNL-SGGDDDGEMNNLQIAIGRITR 835

  Fly   935 SIERVRDLIRKKRQERKDRKERKFAEKFQQIVLDAQQAHAQTLSHQAAVGLERGDKPGVLAETKF 999
            .|:.|:.|:               |...|:|:                     |.||
Zfish   836 GIDWVKALV---------------ASMVQRIL---------------------GKKP-------- 856

  Fly  1000 HRLSYQESMNRPVSGSDFGFQIPLHDGLHTIVDGLEYDDTGDLPEQIQLQAHPLPPTSDSMPPTY 1064
                                            |..:.:..||
Zfish   857 --------------------------------DNTKEEGEGD----------------------- 866

  Fly  1065 ESAMMATTGGSFSSVNGNGTCQNLTPFVQAERRLQHQISSGVSTQQYDSREEATYTESIELLGQY 1129
                                                                      |||
Zfish   867 ----------------------------------------------------------IEL---- 869

  Fly  1130 NSTDTDPYANDQRSGCGSFNRGDSLQDNSSRRYGSEEHDEAFLKYQKSLLTRSPSYRKSLDRLSQ 1194
                   ||
Zfish   870 -------YA-------------------------------------------------------- 871

  Fly  1195 SSGQSQRSLLKSEEAEMRRHSSGQSLNSMSIEQDELLSQQGNLREELLNCDQKELFQFLQEEEEL 1259
                                     ||.:         .:|.:.:.|.||
Zfish   872 -------------------------LNHL---------DEGKMADGLTNC--------------- 887

  Fly  1260 QKGTKLRRISNVMRSRRPSSQMGQPENETMVEHSEFDNIIQSFEKELEEIKRSTTSLERKLSNLS 1324
                             .|..:..|              |...|.::||               .
Zfish   888 -----------------LSPTLTVP--------------IARCESDVEE---------------D 906

  Fly  1325 EPSPAADEATKAIMEHIAIITGASERSAADEVVLPLNPYDSYDLSSVPRRSQSVSAAAQRQSVKL 1389
            |.|.::||                     ::....||..||
Zfish   907 EDSESSDE---------------------EDAKATLNDGDS------------------------ 926

  Fly  1390 KRRSLEKQRKIDEDFSISNEIRKICDQI-HAPFVAMEAMAVAATSASQAQPNQSPFLRRKVDPFT 1453
                                  .:|..: :.|                .:|...|
Zfish   927 ----------------------SVCSTVDYQP----------------PEPEPEP---------- 943

  Fly  1454 VQFDRFKRLSLIERVEEVPEEEKPISTLRIESEKMPRKFLHGPDQLRLDSLSLKSTNSYENLLIQ 1518
                           |||.|||
Zfish   944 ---------------EEVEEEE------------------------------------------- 950

  Fly  1519 KQKLGMATPPAVPATPPTSLKSSIEPPTLAQISSLKTTPPLAALTEHQQHFHATSIQAAPTPAHT 1583
                                                                       |.|
Zfish   951 -----------------------------------------------------------PEP--- 953

  Fly  1584 HAHSQAHAHSMAGQRRRMEHPQSTLDKAASFQSARTESHSSGAADASSALALAMAQKTEQSQSTA 1648
                                                                             
Zfish   954 ----------------------------------------------------------------- 953

  Fly  1649 PDATQKPSAFTRLTEKPWHCLVSYVDDLTVGGRRNSQGAYNDPMTFPSYGATKAAKVPDDCFPQK 1713
                                                                   :.|:.||.:.
Zfish   954 -------------------------------------------------------EEPEACFTEG 963

  Fly  1714 CYDHFYFRCPWFMSCMDTQS----AKHWTRVRTAVLTVVDTPAFEWFVLVLIFASSITLCFEDIN 1774
            |..    ||    :|:....    .|.|..:|....|:|:...||.|::.:|..||..|.|||||
Zfish   964 CIR----RC----ACLSVDITEGWGKKWWNLRRTCFTIVEHDYFETFIIFMILLSSGALAFEDIN 1020

  Fly  1775 LDKNKTLKRVLYWINFSFCLIFVVEMILKWLALGFSKYFTSFWTILDFIIVFVSVFSL---LIEE 1836
            :::.:.:|.:|.:.:..|..||:|||:|||:|.||..|||:.|..|||:||.||:.||   |:..
Zfish  1021 IERRRVIKTILEYADKVFTYIFIVEMLLKWVAYGFKTYFTNAWCWLDFLIVDVSLVSLTANLMGY 1085

  Fly  1837 NENLKVLRSLRTLRALRPLRAISRWQGMRIVVNALMYAIPSIFNVLLVCLVFWLIFSIMGVQFFG 1901
            :| |..::|||||||||||||:||::|||:|||||:.|||||||||||||:||||||||||..|.
Zfish  1086 SE-LGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIFNVLLVCLIFWLIFSIMGVNLFA 1149

  Fly  1902 GKFFKCVN-EMGELLPITEVNDKWDCIEQNYT----WINSKITFDHVGMGYLALLQVATFEGWME 1961
            |||:.|:| ...|.:|:..||:|.||:...||    |:|.|:.:|:||:|||:|||:|||:|||:
Zfish  1150 GKFYHCINTTTEERIPMDVVNNKSDCMALMYTNEVRWVNVKVNYDNVGLGYLSLLQIATFKGWMD 1214

  Fly  1962 VMADAVDARGVDLQPQREANLYAYIYFVIFIVCGSFFTLNLFIGVIIDNFNMLKKKYEGGVLEMF 2026
            :|..|||:|.||.||..|.|||.|:||||||:.||||||||||||||||||..|.|:.|  .::|
Zfish  1215 IMYAAVDSREVDEQPSYEINLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKSKFGG--KDIF 1277

  Fly  2027 LTESQKHYYTAMKKLGRKKPQKVIKRPINHFLAMFYDLSNSRRFEIAIFVLIFLNMLTMGIEHYD 2091
            :||.||.||.||||||.||..|.|.||.|....:.:|..:.:.|:|.|.|||.|||:||.||..|
Zfish  1278 MTEEQKKYYNAMKKLGAKKRPKPIPRPSNIIQGLVFDFISKQFFDIFIMVLICLNMVTMMIETDD 1342

  Fly  2092 QPHAVFFILEVSNAFFTTVFGLEAIVKIVGLRYHYFTVPWNVFDFLLVLASIFGILMEDIMIDLP 2156
            |.....::|...|..|..||..|.::|:..||.::||:.||||||::|:.||.|:::.||:....
Zfish  1343 QSAEKEYVLYQINLVFIVVFTSECVLKLFALRQYFFTIGWNVFDFVVVILSIAGLMLSDIIEKYF 1407

  Fly  2157 ISPTLLRVVRVFRIGRILRLIKAAKGIRKLLFALVVSLPALFNIGALLGLITFIYAILGMSLFGN 2221
            :||||.||:|:.||||:||||:.|||||.|||||::|||||||||.||.||.||::|.|||.|..
Zfish  1408 VSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLIMFIFSIFGMSNFAY 1472

  Fly  2222 VKLQGALDDMVNFQTFGRSMQLLFRLMTSAGWNDVLESLMIQ-PPDCDP-FIHGHTN--GNCGHP 2282
            ||.|..:||:.||:|||.|:..||.:.|||||:.:|..::.. |||||| |.:..|:  ||||:|
Zfish  1473 VKKQAGIDDIFNFETFGGSIICLFEITTSAGWDGLLLPILNSGPPDCDPDFENPGTDVRGNCGNP 1537

  Fly  2283 LLAITYFTSFIIISYMIVINMYIAIILENFNQAHQEEEIGIVEDDLEMFYIRWSKYDPHATQFIH 2347
            .:.|.:|.|:||:|:::|:||||||||||||.|.:|....:.|||.:||...|.|:|..|||||.
Zfish  1538 GMGIMFFCSYIIMSFLVVVNMYIAIILENFNNAQEESGDPLCEDDFDMFDETWEKFDVDATQFIE 1602

  Fly  2348 FSQLSDFIASLDPPLGISKPNNVALVSFNLPISKGNKIHCLDILHALVKHVLGHVEETDNFKQLQ 2412
            :.:|.||:.:|..||.|:|||.:.|:|.::||..|:|||..|||.|:.:.|||...|.|..|
Zfish  1603 YDRLFDFVDALQEPLRIAKPNRLKLISMDIPIVNGDKIHSQDILLAVTREVLGDTIEMDAMK--- 1664

  Fly  2413 EQMDVKFKKQFPTRKELEIVSSTRIWKRQEKAAKTIQTGWKEYLRRKREKERSNSGDSATQTSSP 2477
            |.::.||....||....|.:.:|...|.:|:||..:|..::.:|.::
Zfish  1665 ESIEAKFIMNNPTSASFEPIITTLRRKEEERAAIAVQRIYRRHLLKR------------------ 1711

  Fly  2478 GGWQSKLSALNFFHLQVSRRGTACSSRASSRKSSRASD----------ASDLSELAG--PWLNLP 2530
                    |:.:          ||..|.|.||....:|          |..::.|.|  |.|.:.
Zfish  1712 --------AIRY----------ACFMRQSKRKVRNPNDNEPPETEGLIARKMNTLYGSNPELAMA 1758

  Fly  2531 LMLVSGADEVVKDIKQQNDELGKRGSIFVEAPRASRRRSFYNFFLRHQDAVDDSLTSPSVHRKTA 2595
            |.|.:..........:.:.....|.|:....|:.             |..:...|||..:.|...
Zfish  1759 LELETRPMRPNSQPPKPSQVTQTRASVTFPRPQG-------------QLILPVELTSEVILRSAP 1810

  Fly  2596 MNNTTNTTSNSAS 2608
            ..::.|::.|:.:
Zfish  1811 TTHSFNSSENATT 1823

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NaCP60E	NP_001261172.1	Ion_trans	125..371	CDD:459842	116/268 (43%)
		Ion_trans	687..902	CDD:459842	122/218 (56%)
		Ion_trans	1751..2019	CDD:459842	161/275 (59%)
		Na_channel_gate	2009..2063	CDD:240441	27/53 (51%)
		Ion_trans	2067..2320	CDD:459842	136/256 (53%)
		GPHH	2332..2378	CDD:465306	20/45 (44%)
scn4aa	NP_001034914.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	32..52		3/22 (14%)
		I. /evidence=ECO:0000305	106..421		141/317 (44%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	446..468		7/40 (18%)
		II. /evidence=ECO:0000305	564..836		143/278 (51%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	901..957		22/403 (5%)
		III. /evidence=ECO:0000305	979..1292		177/315 (56%)
		Important for rapid channel inactivation. /evidence=ECO:0000250|UniProtKB:P15390	1276..1278		0/1 (0%)
		IV. /evidence=ECO:0000305	1301..1599		150/297 (51%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1765..1786		2/20 (10%)