Protein Alignment NaCP60E and scn8a

DIOPT Version :10

• Sequence 1: NP_001261172.1
• Gene: NaCP60E / 37981
• FlyBaseID: FBgn0085434
• Length: 2896
• Species: Drosophila melanogaster
• Sequence 2: XP_031751845.1
• Gene: scn8a / 549640
• XenbaseID: XB-GENE-1011299
• Length: 1987
• Species: Xenopus tropicalis
• Alignment Length: 2631
• Identity: 823/2631 - (31%)
• Similarity: 1173/2631 - (44%)
• Gaps: 859/2631 - (32%)

  Fly    45 PRTKASVEDGDVLPRKFEPFPEHMYGKPLEEIDTF-IYEETFCVVSKRFRKNYIHRFTGTKSLFL 108
            ||..:.:|.|..:|..:...|..:..:|:|::|.| ::.:||.|::   |...|.||:...:|::
 Frog    53 PRPNSDLEAGKGVPFIYGDCPSRLIAEPIEDLDPFYLHTKTFIVLN---RGKTIFRFSAAPALYI 114

  Fly   109 FYPWSPARRVCVYIATNQFFDYCVMATILFNCIFLAMT---ETVEEAEYIFLAIYSIEMVIKIIA 170
            ..|::|.|:..:.|..:..|...:|.|||.||:|:..:   |..::.||.|..||:.|.::||||
 Frog   115 LSPFNPLRKFAIKILIHSLFSMIIMCTILTNCVFMTFSNPPEWSKQVEYTFTGIYTFESLVKIIA 179

  Fly   171 KGFLLNKYTYLRNPWNWLDFVVITSGYATIGMEVGNLAGLRTFRVLRALKTVSIMPGLKTIINAL 235
            :||.::.:|:||:|||||||.||...|.|..:::||::.||||||||||||:|::||||||:.||
 Frog   180 RGFCIDGFTFLRDPWNWLDFSVIMMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGAL 244

  Fly   236 LHSFRQLAEVMTLTIFCLMVFALFALQVYMGELRNKCVRQVPTDWTNVSHTDWQIWVNDTDNWLY 300
            :.|.::|::||.||:|||.||||..||::||.||.|||.. |.|      .|.....|.:..:.:
 Frog   245 IQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLRRKCVAW-PVD------MDQMYLANGSKGFDF 302

  Fly   301 DEDEL-------------PVLCGNLTGARHCPFEYVCLCVGENPNHGYTNFDNFMWSMLTTFQLI 352
            ||..:             |:||||.:.|..||..|.|:..|.|||:|||:||.|.|:.|..|:|:
 Frog   303 DEYIMNDTNFFKVHGAIDPLLCGNGSDAGQCPEGYTCMKAGRNPNYGYTSFDTFSWAFLALFRLM 367

  Fly   353 TLDYWENVYNMVLATCGPMSVSFFTVVVFFGSFYLINLMLAVVALSYEEEAEITNEERKKDLLDH 417
            |.|:|||:|.:.|...|...:.||.:|:|.|||||:||:|||||::|||:.:.|.||.::
 Frog   368 TQDFWENLYQLTLRASGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAER----- 427

  Fly   418 RDDSTFSFDPSVLNVKKLNKNNKKKIDSRKGVLLASYSKKKTRRKKTKGGKEGG--TNGNGNGSN 480
                               |.|:.|      .:|....:.:...:........|  |.......:
 Frog   428 -------------------KENEFK------AMLEQMKRMQEEAQAAANATSAGTVTEDAVEDED 467

  Fly   481 GDDNKSHSATPSPGPSPRHSATERPSALTMQAQKQYQQMEQQ---HKLAKSGSGGSNNPMAPTPK 542
            |:...||:::....||.: ||.||.:....:.||:..:.|::   .|:.||
 Frog   468 GEARMSHTSSEFSKPSSK-SAKERRNRRKKRKQKELSEGEEKGDSEKVYKS-------------- 517

  Fly   543 GRISFQDSGMGVKNPNMLYPSDYKGQ--------LIANSGQP-----SSNSS-----GVNRESSQ 589
                  :|..|||.....||.:..|:        |::..|.|     :|.||     |..|:...
 Frog   518 ------ESEDGVKRRGFWYPDNRLGRRFSIMNQSLLSIPGSPYLSRHNSKSSIFSFRGRFRDPVS 576

  Fly   590 DDSGVVDDH---EEQDTTND------MGH---------------VSTV--ELALSPREVRLIKCN 628
            ::....|:|   ||.:...|      .||               .|.:  .|..:|:....:.||
 Frog   577 ENEFADDEHSTVEESEGRRDSLFIPVRGHDRRNSYNSGYSQGSRSSRILQNLRRNPKRNSTVDCN 641

  Fly   629 GNIARI--KNHNVYA--LHQEFSSEVVVIDDLP-------------------------------- 657
            |.::.|  ...|:..  |..|...:....||.|
 Frog   642 GVVSLIGGPGSNIPGGRLLPEVKIDKAATDDSPTTEVEVKRKPSGSLRVSMDQLTASFGRKRANS 706

  Fly   658 ----------------DRNCDRCVH--------WCTDYESWLQFQNCLYKVVRDPLFELAITLCI 698
                            .|.|..|.:        | |....|::.:..:..:|.||..:|.||:||
 Frog   707 ILSVVTQTLVEELEESQRKCPPCWYKFADTYLIW-TCCPLWIRIKTIVNMIVMDPFVDLTITICI 770

  Fly   699 VLNTAFLAMEHHGMSESFRNALDVGNKVFTSIFTFECIVKLMALSK-DFFLCGWNIFDLLIVTAS 762
            ||||.|:||||:.|:..|.|.|.|||.|||.|||.|..:||:|:.. .:|..||||||..||:.|
 Frog   771 VLNTLFMAMEHYPMTPHFENVLVVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGFIVSLS 835

  Fly   763 LLDIIFELVDGLSVLRGLRLLRVLKLAQSWTTMKVLLSIIISTIGALGNLTLILVIVIYIFAVIG 827
            |:::..:.|:||||||..|||||.|||:||.|:.:|:.||.:::||||||||:|.|:::||||:|
 Frog   836 LMELGLQDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVG 900

  Fly   828 MQLFSKDYTP--EKFDPDPV-PRWNFNDFFHSFMMIFRILCGEWIEPLWDCMRAEEEQGASTCFA 889
            ||||.|.|..  .|.:|:.| |||:.|||||||:::||:|||||||.:||||   |..|.:.|..
 Frog   901 MQLFGKSYKDCVCKINPECVLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCM---EVAGQAMCII 962

  Fly   890 IFLPTLVMGNFMVLNLFLALLLNSFNSEELKSKKEEVGEESKLARSIERVRDLIRKKRQERKDRK 954
            :|:..:|:||.:||||||||||:||:::.|.:..:: ||.:.|..|:.|::..|...:.:..|..
 Frog   963 VFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDD-GEMNNLQISVIRIKKGIAWIKLKAHDFM 1026

  Fly   955 ERKFAEKFQQIVLDAQQAHAQTLSHQAAVGLERGDKPGVLAETKFHRLSYQESMN--RPVSGSDF 1017
            ::.|.:|      ||.:.                 ||  |.|      .|::.||  ...:|:|
 Frog  1027 QKHFKKK------DADEV-----------------KP--LDE------MYEKKMNCLANNTGAD- 1059

  Fly  1018 GFQIPLHDGLHTIVDGLEYDDTGDLPEQIQLQAHPLPPTSDSMPPTYESAMMATTGGSFSSVNGN 1082
                 :|..:             |.|:                                   |||
 Frog  1060 -----IHRDM-------------DYPK-----------------------------------NGN 1071

  Fly  1083 GTCQNLTPFVQAERRLQHQISSGVSTQQYDSREEATYTESIELLGQYNSTDTDPYANDQRSGCGS 1147
            ||                                                         .||.||
 Frog  1072 GT---------------------------------------------------------TSGIGS 1079

  Fly  1148 FNRGDSLQDNSSRRYGSEEHDEAFLKYQKSLLTRSPSYRKSLDRLSQSSGQSQRSLLKSEEAEMR 1212
                      |..:|..:|...:|: :..:|..:.|
 Frog  1080 ----------SVEKYMIDEDHMSFI-HNPNLTVKVP----------------------------- 1104

  Fly  1213 RHSSGQSLNSMSIEQDELLSQQGNLREELLNCDQKELFQFLQEEEELQKGTKLRRISNVMRSRRP 1277
                                                                             
 Frog  1105 ----------------------------------------------------------------- 1104

  Fly  1278 SSQMGQPENETMVEHSEFDNIIQSFEKELEEIKRSTTSLERKLSNLSEPSPAADEATKAIMEHIA 1342
                      ..|..|:|:|:                       |..:.|.|:||
 Frog  1105 ----------IAVGESDFENL-----------------------NTEDFSSASDE---------- 1126

  Fly  1343 IITGASERSAADEVVLPLNPYDSYDLSSVPRRSQSVSAAAQRQSVKLKRRSLEKQRKIDEDFSIS 1407
              .|:.:                                                 |:|:
 Frog  1127 --EGSKD-------------------------------------------------KLDD----- 1135

  Fly  1408 NEIRKICDQIHAPFVAMEAMAVAATSASQAQPNQSPFLRRKVDPFTVQFDRFKRLSLIERVEEVP 1472
                                    ||:|:.
 Frog  1136 ------------------------TSSSEG----------------------------------- 1141

  Fly  1473 EEEKPISTLRI--ESEKMPRKFLHGPDQLRLDSLSLKSTNSYENLLIQKQKLGMATPPAVPATPP 1535
                  ||:.|  |:|::|
 Frog  1142 ------STIDIKPEAEEVP---------------------------------------------- 1154

  Fly  1536 TSLKSSIEPPTLAQISSLKTTPPLAALTEHQQHFHATSIQAAPTPAHTHAHSQAHAHSMAGQRRR 1600
                                                                             
 Frog  1155 ----------------------------------------------------------------- 1154

  Fly  1601 MEHPQSTLDKAASFQSARTESHSSGAADASSALALAMAQKTEQSQSTAPDATQKPSAFTRLTEKP 1665
            :|.|:..||
 Frog  1155 VEQPEEYLD-------------------------------------------------------- 1163

  Fly  1666 WHCLVSYVDDLTVGGRRNSQGAYNDPMTFPSYGATKAAKVPDDCFPQKCYDHFYFRCPWFMSC-- 1728
                                                    ||:||.:.|...  |:|     |
 Frog  1164 ----------------------------------------PDNCFTEGCVAR--FKC-----CQV 1181

  Fly  1729 -MDTQSAKHWTRVRTAVLTVVDTPAFEWFVLVLIFASSITLCFEDINLDKNKTLKRVLYWINFSF 1792
             ::....|.|..:|.....:|:...||.|::.:|..||..|.|||:.:::.||::.:|.:.:..|
 Frog  1182 SVEEGLGKSWWTLRKTCFLIVEHNWFETFIIFMILLSSGALAFEDVYIEQRKTIRTILEYADKVF 1246

  Fly  1793 CLIFVVEMILKWLALGFSKYFTSFWTILDFIIVFVSVFSLLIEE--NENLKVLRSLRTLRALRPL 1855
            ..||::||:|||||.||.|:||:.|..|||:||.||:.||:...  ...|..::|||||||||||
 Frog  1247 TYIFILEMLLKWLAYGFVKFFTNAWCWLDFLIVDVSLVSLIANALGYSELGAIKSLRTLRALRPL 1311

  Fly  1856 RAISRWQGMRIVVNALMYAIPSIFNVLLVCLVFWLIFSIMGVQFFGGKFFKCVNEMGE-LLPITE 1919
            ||:||::|||:|||||:.|||||.|||||||:||||||||||..|.||::.|.||..| :..|.:
 Frog  1312 RALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYYCYNETAEGMFEIED 1376

  Fly  1920 VNDKWDC---IEQNYT---WINSKITFDHVGMGYLALLQVATFEGWMEVMADAVDARGVDLQPQR 1978
            |.::..|   |..|||   |.|.||.||:||.|||||||||||:|||::|..|||:|.|..||:.
 Frog  1377 VENETQCFDLIGNNYTEVRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKVHQQPKY 1441

  Fly  1979 EANLYAYIYFVIFIVCGSFFTLNLFIGVIIDNFNMLKKKYEGGVLEMFLTESQKHYYTAMKKLGR 2043
            |.|:|.||||||||:.||||||||||||||||||..|||:.|  .::|:||.||.||.||||||.
 Frog  1442 EDNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGG--QDIFMTEEQKKYYNAMKKLGS 1504

  Fly  2044 KKPQKVIKRPINHFLAMFYDLSNSRRFEIAIFVLIFLNMLTMGIEHYDQPHAVFFILEVSNAFFT 2108
            |||||.|.||:|.|....:|:...:.|:|.|.:||.|||:||.:|..||......:|...|..|.
 Frog  1505 KKPQKPIPRPLNKFQGAIFDIITQQAFDIVIMILICLNMVTMMVETDDQSDYTDNVLYWINVVFI 1569

  Fly  2109 TVFGLEAIVKIVGLRYHYFTVPWNVFDFLLVLASIFGILMEDIMIDLPISPTLLRVVRVFRIGRI 2173
            ..|..|.::|:..||::|||:.||:|||::|:.||.|:.:.|::....:||||.||:|:.|||||
 Frog  1570 VFFTTECVLKLCALRHYYFTIGWNIFDFVVVILSIVGMFLADLITKYFVSPTLFRVIRLARIGRI 1634

  Fly  2174 LRLIKAAKGIRKLLFALVVSLPALFNIGALLGLITFIYAILGMSLFGNVKLQGALDDMVNFQTFG 2238
            |||||.|||||.|||||::|||||||||.||.|:.||::|.|||.|..||.:..:|||.||:|||
 Frog  1635 LRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKKEAGIDDMFNFETFG 1699

  Fly  2239 RSMQLLFRLMTSAGWNDVLESLMIQPPDCDPFIH-----GHTNGNCGHPLLAITYFTSFIIISYM 2298
            .||..||::.|||||:.:|..::.:|||||  .|     ....|:||:|.:.|.:|.|:||||::
 Frog  1700 NSMICLFQITTSAGWDGLLLPILNRPPDCD--AHKENPGSQVKGDCGNPSVGIFFFVSYIIISFL 1762

  Fly  2299 IVINMYIAIILENFNQAHQEEEIGIVEDDLEMFYIRWSKYDPHATQFIHFSQLSDFIASLDPPLG 2363
            ||:|||||||||||:.|.:|....:.|||.|.||..|.|:||.|||||.||:|.||..:|:.||.
 Frog  1763 IVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIQFSKLPDFADALEHPLR 1827

  Fly  2364 ISKPNNVALVSFNLPISKGNKIHCLDILHALVKHVLGHVEETDNFKQLQEQMDVKFKKQFPTRKE 2428
            |.|||.:.|::.:||:..|::|||||||.|..|.|||...|.|   .|::||:.:|....|::..
 Frog  1828 IPKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGESGELD---ILRQQMEERFVASNPSKVS 1889

  Fly  2429 LEIVSSTRIWKRQEKAAKTIQTGWKEYL------RRKREKERSNSG----DSATQTSSPGGWQSK 2483
            .|.:::|...|:::.:|..||..::.:|      .|||...:..:|    |....|.|.....|.
 Frog  1890 YEPITTTLRRKQEDVSAVVIQQAYRRHLVKRGFISRKRSTGKMENGGTNMDKKEGTPSTASLPSY 1954

  Fly  2484 LSALNFFHLQVSRRGTACSSRASSRKSSRAS 2514
            .|.......:..|...|...||..:||.|.|
 Frog  1955 DSVTKPDKAKQERIEDAIRERAKKQKSVRES 1985

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NaCP60E	NP_001261172.1	Ion_trans	125..371	CDD:459842	118/261 (45%)
		Ion_trans	687..902	CDD:459842	119/218 (55%)
		Ion_trans	1751..2019	CDD:459842	160/276 (58%)
		Na_channel_gate	2009..2063	CDD:240441	31/53 (58%)
		Ion_trans	2067..2320	CDD:459842	132/257 (51%)
		GPHH	2332..2378	CDD:465306	23/45 (51%)
scn8a	XP_031751845.1	Ion_trans	131..421	CDD:459842	137/296 (46%)
		Na_trans_cytopl	545..709	CDD:463401	28/163 (17%)
		Ion_trans	759..972	CDD:459842	117/215 (54%)
		Na_trans_assoc	998..1199	CDD:461936	74/826 (9%)
		Ion_trans	1204..1480	CDD:459842	158/275 (57%)
		Na_channel_gate	1472..1524	CDD:240441	31/53 (58%)
		Ion_trans	1529..1783	CDD:459842	131/255 (51%)