Protein Alignment NaCP60E and nalcn

DIOPT Version :10

• Sequence 1: NP_001261172.1
• Gene: NaCP60E / 37981
• FlyBaseID: FBgn0085434
• Length: 2896
• Species: Drosophila melanogaster
• Sequence 2: XP_005167755.1
• Gene: nalcn / 548345
• ZFINID: ZDB-GENE-050410-12
• Length: 1743
• Species: Danio rerio
• Alignment Length: 2581
• Identity: 452/2581 - (17%)
• Similarity: 780/2581 - (30%)
• Gaps: 1097/2581 - (42%)

  Fly   106 LFLFYPWSPARRVCVYIATNQFFDYCVMATILFNCIFLAMT-ETVEEAEYIFLA-------IYSI 162
            |::..||           .:.....|.:.:::..|:....| |.....:|:..|       :|:.
Zfish    56 LWINKPW-----------VHSLLRACAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTA 109

  Fly   163 EMVIKIIAKGFLLNKYTYLRNPWNWLDFVVITSGYATIGMEVGNLAGL-------RTFRVLRALK 220
            ||:.|:..:|.:..:.:|:::.|...|..::...:.::.::|..:|.|       ...|:.|||.
Zfish   110 EMIAKMHIRGIIKGENSYVKDRWCMFDGFMVVFLWVSLVLQVFEIAELVDQMSPWGMLRIPRALI 174

  Fly   221 TVSI--------MPGLKTIINALLHSFRQLAEVMTLTIFCLMVFALFALQVYMGELRNKCVRQVP 277
            .:.:        :|..: |.|.|..|..|:..|....:|.|:::.:..:|:: |...:.||
Zfish   175 MIRVFRIYFRFELPRSR-ITNILKRSGEQIWSVTIFLLFFLLLYGILGVQMF-GTFNHHCV---- 233

  Fly   278 TDWTNVSHTDW-QIWVNDTDNWLYDEDELPVLCGNLTGARHCPFEYVCLCVGE----NPNHGYTN 337
            |:.|......| .:.:.||.            |........||..:.|:.:.:    ....||:.
Zfish   234 TNETEKEKVTWNSLAIPDTH------------CSPNGEGYQCPVGFKCVDLEDYGLSRQELGYSG 286

  Fly   338 FDNFMWSMLTTFQLITLDYWENV-YNMVLATCGPMSVSFFTVVVFFGSFYLINLMLAVVALSYEE 401
            |:....|:.|.::..:.:.|..: |..:.:.....|..:|..::||.::.:.|:.:||:   .|.
Zfish   287 FNELGTSIFTVYEAASQEGWVFIMYRAIDSFPRWRSYFYFLTLIFFLAWLVKNVFIAVI---IET 348

  Fly   402 EAEITNEERKKDLLDHRDDSTFSFDPSVLNVKKLNKNNKKKIDSRKGVLLASYSKKKTRRKKTKG 466
            .|||
Zfish   349 FAEI------------------------------------------------------------- 352

  Fly   467 GKEGGTNGNGNGSNGDDNKSHSATPSPGPSPRHSATERPSALTMQAQKQYQQMEQQHKLAKSGSG 531
                                                          :.|:|||        .||.
Zfish   353 ----------------------------------------------RVQFQQM--------WGSR 363

  Fly   532 GSNNPMAPTPKGRISFQDSGMGVKNPNMLYPSDYKG--QLIANSGQPSSNSSGVNRESSQDDSGV 594
            .|....|.|                  .::..|..|  ||:|
Zfish   364 SSTTSTATT------------------QMFHEDASGGWQLVA----------------------- 387

  Fly   595 VDDHEEQDTTNDMGHVSTVELALSPREVRLIKCNGNIARIKNHNVYALHQEFSSEVVVIDDLPDR 659
                                       |.:.|.:|....
Zfish   388 ---------------------------VDVNKPHGRAPA-------------------------- 399

  Fly   660 NCDRCVHWCTDYESWLQFQNCLYKVVRDPLFELAITLCIVLNTAFLAMEHH-GMSESFRNALDVG 723
                                ||.:::|..:|.:.|...:.::....|..:| |..:...:...:.
Zfish   400 --------------------CLQQLMRSSVFHMFILSMVAVDVIVAASNYHRGDHKLTNDEFYLA 444

  Fly   724 NKVFTSIFTFECIVKLMALS-KDFFLCGWNIFDLLIVTASLLDIIFELVDG-LSVLRGLRLLRVL 786
            ...||.:|..|.::|:..|. ..:.....:.|:.|:|..:.|.|..:|... .:..:.||::|::
Zfish   445 EVAFTVLFDLEALLKIWCLGFTGYISSSLHKFESLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLI 509

  Fly   787 KLAQSWTTMKVLLSIIISTIG---ALGNLTLILVIVIYIFAVIGMQLFSKDYTPEKFDPDPVPRW 848
            |::.:      |...:....|   .||:|.:....::.:.:.|.:|:|.   ..|..|
Zfish   510 KISPA------LEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFC---FVEDLD------- 558

  Fly   849 NFNDFFHSFMMIFRILCGE-WIEPLWDCMRAEEEQGASTCFAIFLPTLVMGNFMVLNLFLALLLN 912
            .|..|..:||.:|:||..| |::.:...:.|..:..|......|:...:....::|:||:|::|:
Zfish   559 RFTTFPRAFMSMFQILTQEGWVDVMDQTLVAVGQMWAPVVAIYFILYHLFATLILLSLFVAVILD 623

  Fly   913 SFN-SEELKSKKEEVGEESKLARSIERVRDLIRKKRQERKDRKER-----KFAEKFQQIVLDAQQ 971
            :.. .|:||..|:                   .|:.:...|.||:     :..|||.
Zfish   624 NLELDEDLKKLKQ-------------------LKQSEANADTKEKLPLRLRIFEKFP-------- 661

  Fly   972 AHAQTLSHQAAVGLERGDKPGVLAETKFHRLSYQESMNRPVSGSDFGFQIPLHDGLHTIVDGLEY 1036
                                                 ||
Zfish   662 -------------------------------------NR-------------------------- 663

  Fly  1037 DDTGDLPEQIQLQAHPLPPTSDSMPPTYESAMMATTGGSFSSVNGNGTCQNLTPFVQAERRLQHQ 1101
                  |:.:::...|    ||...|....:.|
Zfish   664 ------PQMVKISKLP----SDFTVPRIRESFM-------------------------------- 686

  Fly  1102 ISSGVSTQQYDSREEATYTESIELLGQYNSTDTDPYANDQRSGCGSFNRGDSLQDNSSRRYGSEE 1166
                   :|:..|::                                      ||.|
Zfish   687 -------KQFIDRQQ--------------------------------------QDPS-------- 698

  Fly  1167 HDEAFLKYQKSLLTRSPSYRKSLDRLSQSSGQSQRSLLKSEEAEMRRHSSGQSLNSMSIEQDELL 1231
                      .|..|.||      ..|.|...|:||                     :||.::.:
Zfish   699 ----------CLFRRLPS------ASSSSCDHSKRS---------------------AIEDNKYI 726

  Fly  1232 SQQGNLREELLNCDQKELFQFLQEEEELQKGTKLRRISNVMRSRRPSSQMGQPENETMVEHSEFD 1296
            .|                              |||:....:|:|
Zfish   727 DQ------------------------------KLRKSVFSIRAR--------------------- 740

  Fly  1297 NIIQSFEKEL--EEIKRSTTSLERKLSNLSEPSPAADEATKAIMEHIAIITGASERSAADEVVLP 1359
            |::   |||:  .:|.|:.|. :|.||...|..|..:.:..::..||
Zfish   741 NLM---EKEITVNKILRACTR-QRMLSGSFEGQPTKERSILSVQHHI------------------ 783

  Fly  1360 LNPYDSYDLSSVPRRSQSVSAAAQRQSVKLKRRSLEKQRKIDEDFSISNEIRKICDQIHAPFVAM 1424
                      ...|||....:.:||.|   :.:|||   .:.:|.|
Zfish   784 ----------RQERRSLRHGSTSQRIS---RGKSLE---TLTQDHS------------------- 813

  Fly  1425 EAMAVAATSASQAQPNQSPFLRRKVDPFTVQFDRFKRL-SLIERVEEVPEEEKPISTLRIESEKM 1488
                                       .||::...:|. |.|:.::|           :.|..:|
Zfish   814 ---------------------------STVRYRNAQREDSEIKMIQE-----------KKEQAEM 840

  Fly  1489 PRKFLHGPDQLRLDSLSLKSTNSYENLLIQKQKLGMATPPAVPATPPTSLKSSIEPPTLAQISSL 1553
            .||                         :|:::|
Zfish   841 KRK-------------------------VQEEEL------------------------------- 849

  Fly  1554 KTTPPLAALTEHQQHFHATSIQAAPTPAHTHAHSQAHAHSMAGQRRRMEHPQSTLDKAASFQSAR 1618
                                                          |..||
Zfish   850 ----------------------------------------------RENHP-------------- 854

  Fly  1619 TESHSSGAADASSALALAMAQKTEQSQSTAPDATQKPSAFTRLTEKPWHCLVSYVDDLTVGGRRN 1683
                                                      ..:||          |.:.||.:
Zfish   855 ------------------------------------------YFDKP----------LFIVGREH 867

  Fly  1684 S---------QGAYNDPMTFPSYGATKAAKVPDDCFPQKCYDHFYFRCPWFMSCMDTQSAKHWTR 1739
            .         :..:|...|.|:.||..:.|.      .:.||
Zfish   868 RFRNFCRMIVRARFNASNTDPNTGAVNSTKY------HQLYD----------------------- 903

  Fly  1740 VRTAVLTVVDTPAFEWFVLVLIFASSITLCFEDINLDKNKTLKRVLYWI-----NFSFCLIFVVE 1799
             ...::|.:|     |.::|:...|.|::.||       ....||::..     .:.|.:...:|
Zfish   904 -LLGLVTYLD-----WVMIVVTICSCISMMFE-------SPFTRVMHVPTLQIGEYVFVIFMSIE 955

  Fly  1800 MILKWLALGFSKYFTSFWTILDF---IIVFVSVFSLLI----------EENENLKVLRSLRTLRA 1851
            :.||.:|.|.  :||....|.||   :.:|:.:.||:.          |....|     |..||.
Zfish   956 LNLKIMADGL--FFTPTAVIRDFGGVMDIFIYLVSLIFLCWLPNNVPPESGAQL-----LMMLRC 1013

  Fly  1852 LRPLRAISRWQGMRIVVNALMYAIPSIFNVLLVCLVFWLIFSIMGVQFFGGKFFKCVNEMGELLP 1916
            .||||.......||.||..::.....||.|.::.|...|:|:..|||.|.||..|| |:
Zfish  1014 FRPLRIFKLVPQMRKVVREVLKGFKEIFLVSILLLTLMLVFATFGVQLFAGKLAKC-ND------ 1071

  Fly  1917 ITEVNDKWDC---------IEQNY-----------------TWINSK-ITFDHVGMGYLALLQVA 1954
             ..::.|.||         |.:|.                 .|.|.: ..||:||...|||.:|.
Zfish  1072 -PHISSKDDCHGIFRINVSISKNLNLKLKDGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVL 1135

  Fly  1955 TFEGWMEVMADAVDARGVDLQPQREANLYAYIYFVIFIVCGSFFTLNLFIGVIIDNFNMLKKKYE 2019
            :.:||:||.         |:...|...::. ||..:|:..|....|.||:||:|.|||    :.:
Zfish  1136 SLKGWVEVR---------DVIIHRVGPIHG-IYIHVFVFLGCMIGLTLFVGVVIANFN----ENK 1186

  Fly  2020 GGVLEMFLTESQKHYYTAMKKLGRKKPQKVIKRPIN-HFLAMFYDLSNSRRFEIAIFVLIFLNML 2083
            |..|   ||..|:.:.....:|...:|..:..||.| .|.|..||::....|:..|.||:....:
Zfish  1187 GTAL---LTVDQRRWEDLKSRLKIAQPLHLPPRPENGGFRAKMYDITQHPFFKRGIAVLVLAQSV 1248

  Fly  2084 TMGIEHYDQPHAVFFILEVSNAFFTTVFGLEAIVKIVGLR-YHYFTVPWNVFDFLLVLASIFGIL 2147
            .:.:: :|:..||.|.|...:..||.:|.||..:|::.:. ..|:....|.:|.|:....:..|:
Zfish  1249 LLSVK-WDELGAVTFPLATMSVVFTFIFVLEVTMKLIAMSPAGYWQSRRNRYDLLVTSLGLIWII 1312

  Fly  2148 MEDIMID--LPISPTLLRVVRVFRI-GRILRLIKAAKGIRKLLFALVVSLPALFNIGALLGLITF 2209
            :...:.:  ..:..|.:.|.|.|.| |:.:.|       :.||..:|||:...|.|...:.|:..
Zfish  1313 LHFALQNAYTYMMGTCVIVFRFFTICGKHVTL-------KMLLLTVVVSMYKSFFIIVGMFLLLL 1370

  Fly  2210 IYAILGMSLFGNVKLQGALDDMVNFQTFGRSMQLLFRLMTSAGWNDVLESLMIQPPDCDPFIHGH 2274
            .||..|:.|||.||....::...||.|.|:::.:|.|::|...||.::...|:|||.|.|..|.:
Zfish  1371 CYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLIRIVTGEDWNKIMHDCMVQPPFCTPDKHRY 1435

  Fly  2275 TNGNCGHPLLAITYFTSFIIISYMIVINMYIAIILENFNQAHQEEEIGIVE-DDLEMFYIRWSKY 2338
            ...:||:...|:.||.||.:|...|::|:.:|||:|||:..:..||..::. :||..|.|.|:..
Zfish  1436 WETDCGNYAGALIYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMV 1500

  Fly  2339 DPHATQFIHFSQLSDFIASLDPPLGISKPNNVALVSFNLPISKGNKIHCLDILHALVKHVLGHVE 2403
            |......|..|:                      |.|.|.:.:|.....||....|.||:...:|
Zfish  1501 DDKREGVIPTSR----------------------VKFLLRLLRGRLEVDLDKDKLLFKHMCYEME 1543

  Fly  2404 ETDN-----FKQL-----QEQMDVKFKKQFP---TRKELEIVSSTRIWKRQEKAAKTIQTGWKEY 2455
            ...|     |..:     ...:|::...|..   .|::||...      .:|.|.:||:...|:.
Zfish  1544 RLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTI------EEEVAKQTIRMWLKKC 1602

  Fly  2456 LRRKREKER------------------------------------SNSGDSATQTSSPGGWQSKL 2484
            |:|.|.|::                                    :::.|:.||.....|.|
Zfish  1603 LKRIRAKQQQSCSIILSLRESQQQDLRRLLNPPSIETTVPSEDTNTHNQDNPTQPEQNSGLQ--- 1664

  Fly  2485 SALNFFHLQVSRRGTACSSRASSRKSSRASDASDLSELAGPWLNLP 2530
                          |..|...|.|...|...|.......|.| .||
Zfish  1665 --------------TLLSPTLSDRSGYRQDSADRPQRKLGQW-RLP 1695

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NaCP60E	NP_001261172.1	Ion_trans	125..371	CDD:459842	50/274 (18%)
		Ion_trans	687..902	CDD:459842	43/221 (19%)
		Ion_trans	1751..2019	CDD:459842	84/312 (27%)
		Na_channel_gate	2009..2063	CDD:240441	15/54 (28%)
		Ion_trans	2067..2320	CDD:459842	75/256 (29%)
		GPHH	2332..2378	CDD:465306	7/45 (16%)
nalcn	XP_005167755.1	Ion_trans	61..355	CDD:459842	63/432 (15%)
		Ion_trans	407..626	CDD:459842	48/234 (21%)
		Ion_trans	910..1184	CDD:459842	85/314 (27%)
		Ion_trans	1232..1481	CDD:459842	75/256 (29%)