Protein Alignment NaCP60E and scn4a

DIOPT Version :10

• Sequence 1: NP_001261172.1
• Gene: NaCP60E / 37981
• FlyBaseID: FBgn0085434
• Length: 2896
• Species: Drosophila melanogaster
• Sequence 2: XP_017944811.2
• Gene: scn4a / 100492371
• XenbaseID: XB-GENE-982767
• Length: 1849
• Species: Xenopus tropicalis
• Alignment Length: 2537
• Identity: 811/2537 - (31%)
• Similarity: 1138/2537 - (44%)
• Gaps: 815/2537 - (32%)

  Fly    45 PRTKASVEDGDVLPRKFEPFPEHMYGKPLEEIDTFI-YEETFCVVSKRFRKNYIHRFTGTKSLFL 108
            |..|..:|.|..||..:...|..:.|.|:||:|:|. .::||.|::|   ...|.|||.||:|:|
 Frog    47 PTPKNGLEAGKNLPLIYGDPPPELIGIPIEELDSFYSNQKTFIVLNK---GKAIFRFTATKALYL 108

  Fly   109 FYPWSPARRVCVYIATNQFFDYCVMATILFNCIFLAMTET---VEEAEYIFLAIYSIEMVIKIIA 170
            ..|::|.||..:.:..:..|...:|.|||.||:|:.|::.   .:..||.|..||:.|.:|||:|
 Frog   109 LDPFNPIRRGAIKVLIHSLFSLFIMFTILTNCVFMTMSDPPPWSKNVEYTFTGIYTFESLIKILA 173

  Fly   171 KGFLLNKYTYLRNPWNWLDFVVITSGYATIGMEVGNLAGLRTFRVLRALKTVSIMPGLKTIINAL 235
            :||.::.:|:||:|||||||.||...|.|..:::||::.||||||||||||::::||||||:.||
 Frog   174 RGFCIDSFTFLRDPWNWLDFCVIVMAYTTEFVDLGNVSALRTFRVLRALKTITVIPGLKTIVGAL 238

  Fly   236 LHSFRQLAEVMTLTIFCLMVFALFALQVYMGELRNKCVRQVPTD--------------------- 279
            :.|.::||:||.||:|||.||||..||::||.||:||||..|.|
 Frog   239 IQSVKKLADVMILTLFCLAVFALIGLQLFMGNLRHKCVRWPPPDDYFMLYDNSTLNSTLYNDTMI 303

  Fly   280 ------WTNV---SHTDWQIWVNDTDNWLY-DEDELPVLCGNLTGARHCPFEYVCLCVGENPNHG 334
                  .|||   ...||..::||..|:.: |.....:||||.:.|..||..|.|:..|.|||:|
 Frog   304 NSTLSNSTNVFDNDTIDWGAYLNDEANFYFLDGANDALLCGNSSDAGKCPESYTCMKTGRNPNYG 368

  Fly   335 YTNFDNFMWSMLTTFQLITLDYWENVYNMVLATCGPMSVSFFTVVVFFGSFYLINLMLAVVALSY 399
            ||::|:|.|:.|:.|:|:|.|:|||::.:.|...|...:.||.||:|.||||||||:|||||::|
 Frog   369 YTSYDSFNWAFLSLFRLMTQDFWENLFQLTLRAAGKTYMVFFVVVIFLGSFYLINLILAVVAMAY 433

  Fly   400 EEEAEIT-NEERKKDLLDHRDDSTFSFDPSVLNVKKLNKNNKKKIDSRKGVLLASYSKKKTRRKK 463
            .|:.|.| .||.:|:.         .|...:|.:||..:...::..|...|.|.:..||....:.
 Frog   434 AEQNEATLQEEIEKEK---------EFQELLLQLKKHQEEQARRKSSVASVGLLNAEKKNALSEN 489

  Fly   464 TKGGKEGGTNGNGNGSNGDDNKSHSATPSPGPSPRHSATERPSAL-TMQAQKQYQQMEQQHKLAK 527
            |..| :|..|.:.||..               .|:......||.. |..||...|
 Frog   490 TLSG-DGTENMDCNGRT---------------VPKVIIERAPSMEDTSSAQSDQQ---------- 528

  Fly   528 SGSGGSNNPMAPTPKGRISFQDSGMGVKNPNMLYPSDYKGQLIANSGQPSSNSSGVNRESSQDDS 592
                                ::|.:.::.|.:               |..:.|:
 Frog   529 --------------------RNSKVYLEEPTL---------------QKRAEST----------- 547

  Fly   593 GVVDDHEEQDTTNDMGHVSTVELALSPREVRLIKCNGNIARIKNHNVYALHQEFSSEVVVIDDLP 657
                            |....|:||...|....||.            ....:|::..::.|
 Frog   548 ----------------HSVLSEVALEELEEAHQKCP------------PWWYKFANTFLIWD--- 581

  Fly   658 DRNCDRCVHWCTDYESWLQFQNCLYKVVRDPLFELAITLCIVLNTAFLAMEHHGMSESFRNALDV 722
                      |.|.  |::|:..:..||.||..:|.||:||||||.|:||||:.|:|.|...|:|
 Frog   582 ----------CCDL--WVKFKEFVRFVVMDPFVDLGITICIVLNTLFMAMEHYPMTEQFEGVLNV 634

  Fly   723 GNKVFTSIFTFECIVKLMALSK-DFFLCGWNIFDLLIVTASLLDIIFELVDGLSVLRGLRLLRVL 786
            ||.|||.||..|...|::||.. .:|..||||||.:|||.||:::....|:||||||..|||||.
 Frog   635 GNLVFTGIFAAEMFFKIIALDPYYYFQVGWNIFDSIIVTLSLVELGLANVEGLSVLRSFRLLRVF 699

  Fly   787 KLAQSWTTMKVLLSIIISTIGALGNLTLILVIVIYIFAVIGMQLFSKDYTP--EKFDPD-PVPRW 848
            |||:||.|:.:|:.||.:::||||||||:|.|:::||||:|||||.|.|..  .|...| .:|||
 Frog   700 KLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCELPRW 764

  Fly   849 NFNDFFHSFMMIFRILCGEWIEPLWDCMRAEEEQGASTCFAIFLPTLVMGNFMVLNLFLALLLNS 913
            :.:||||||:::||||||||||.:||||   |..|.:.|..:|:..:|:||.:||||||||||:|
 Frog   765 HMHDFFHSFLIVFRILCGEWIETMWDCM---EVAGQAMCLVVFMMVMVIGNLVVLNLFLALLLSS 826

  Fly   914 FNSEELKSKKEEVGEESKLARSIERVR---DLIRKKRQERKDRKERKFAEKFQQIVLDAQQAHAQ 975
            |:::.| |..::.||.:.|..:|.|:.   |.::|
 Frog   827 FSADNL-SASDDDGEMNNLQIAIGRITRGIDFVKK------------------------------ 860

  Fly   976 TLSHQAAVGLERGDKPGVLAETKFHRLSYQESMNRPVSGSDFGFQIPLHDGLHTIVDGLEYDDTG 1040
                                 |.|..|.|:..
 Frog   861 ---------------------TFFSILHYKRK--------------------------------- 871

  Fly  1041 DLPEQIQLQAHPLPPTSDSMPPTYESAMMATTGGSFSSVNGNGTCQNLTPFVQAERRLQHQISSG 1105
                                                               .:||:
 Frog   872 ---------------------------------------------------AKAEQ--------- 876

  Fly  1106 VSTQQYDSREEATYTESIELLGQYNSTDTDPYANDQRSGCGSFNRGDSLQDNSSRRYGSEEHDEA 1170
                    .||....|:|.|    |..:|:|
 Frog   877 --------EEEDPNKENIAL----NHVETEP---------------------------------- 895

  Fly  1171 FLKYQKSLLTRSPSYRKSLDRLSQSSGQSQRSLLKSEEAEMRRHSSGQSLNSMSIEQDELLSQQG 1235
                                                                             
 Frog   896 ----------------------------------------------------------------- 895

  Fly  1236 NLREELLNCDQKELFQFLQEEEELQKGTKLRRISNVMRSRRPSSQMGQPENETMVEHSEFDNIIQ 1300
                              :.:.||:..|                   :||.:
 Frog   896 ------------------EPKIELEPRT-------------------EPEPK------------- 910

  Fly  1301 SFEKELEEIKRSTTSLERKLSNLSEPSPAADEATKAIMEHIAIITGASERSAADEVVLPLNPYDS 1365
                                   .|| |..|..||             :|...||       :|.
 Frog   911 -----------------------KEP-PLIDGVTK-------------KRYYMDE-------FDH 931

  Fly  1366 YDLSSVPRRSQSVSAAAQRQSVKLKRRSLEKQRKIDEDFSISNEIRKICDQIHAPFVAMEAMAVA 1430
            .:..:.|.....|..|::                 :.||.|.:
 Frog   932 ANFFNNPHMRIEVPIASE-----------------ESDFDIPD---------------------- 957

  Fly  1431 ATSASQAQPNQSPFLRRKVDPFTVQFDRFKRLSLIERVEEVPEEEKPISTLRIESEKMPRKFLHG 1495
                              .|.|:.|          ..|||.....|...
 Frog   958 ------------------TDEFSTQ----------SEVEEGILHHKKYD---------------- 978

  Fly  1496 PDQLRLDSLSLKSTNSYENLLIQKQKLGMATPPAVPATPPTSLKSSIEPPTLAQISSLKTTPPLA 1560
                  |..|:.||..|:                    ||       ||
 Frog   979 ------DGSSVCSTADYK--------------------PP-------EP---------------- 994

  Fly  1561 ALTEHQQHFHATSIQAAPTPAHTHAHSQAHAHSMAGQRRRMEHPQSTLDKAASFQSARTESHSSG 1625
              .|.::.|                                ||
 Frog   995 --EEEEEAF--------------------------------EH---------------------- 1003

  Fly  1626 AADASSALALAMAQKTEQSQSTAPDATQKPSAFTRLTEKPWHCLVSYVDDLTVGGRRNSQGAYND 1690
                                                                    :..:|
 Frog  1004 --------------------------------------------------------KEDEG---- 1008

  Fly  1691 PMTFPSYGATKAAKVPDDCFPQKCYDHFYFRCPWFMSCMDTQSAKHWTRVRTAVLTVVDTPAFEW 1755
                           |::||...|..    |||.....:.|:..|.|..:|.....:|:...||.
 Frog  1009 ---------------PEECFTADCVR----RCPCLYVDITTEKGKIWWNIRKTCFQIVEHNWFET 1054

  Fly  1756 FVLVLIFASSITLCFEDINLDKNKTLKRVLYWINFSFCLIFVVEMILKWLALGFSKYFTSFWTIL 1820
            |::.:|..||..|.||||.:::.:.:|.:|.:.:..|..|||.||:|||.|.||..|||:.|..|
 Frog  1055 FIIFMILLSSGALAFEDIYIEQRQVIKTILEYADKVFTYIFVFEMLLKWTAYGFKIYFTNAWCWL 1119

  Fly  1821 DFIIVFVSVFSLLIE--ENENLKVLRSLRTLRALRPLRAISRWQGMRIVVNALMYAIPSIFNVLL 1883
            ||:||.||:.||...  ....|..::|||||||||||||:||::|||:|||||:.|||||.||||
 Frog  1120 DFLIVDVSLISLTANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLL 1184

  Fly  1884 VCLVFWLIFSIMGVQFFGGKFFKCVN-EMGELLPITEVNDKWDCIEQNYT----WINSKITFDHV 1943
            |||:||||||||||..|.||:::|:| ...||..|..||::.||:..|.:    |:|.|:.||:|
 Frog  1185 VCLIFWLIFSIMGVNLFAGKYYRCINTTTSELFEIDVVNNRSDCLALNNSEQVRWVNVKVNFDNV 1249

  Fly  1944 GMGYLALLQVATFEGWMEVMADAVDARGVDLQPQREANLYAYIYFVIFIVCGSFFTLNLFIGVII 2008
            |:|||:|||||||:|||::|..|||:|.::.|||.|.|||.|:||||||:.|:||||||||||||
 Frog  1250 GLGYLSLLQVATFKGWMDIMYAAVDSRNIEDQPQYEINLYMYLYFVIFIIFGAFFTLNLFIGVII 1314

  Fly  2009 DNFNMLKKKYEGGVLEMFLTESQKHYYTAMKKLGRKKPQKVIKRPINHFLAMFYDLSNSRRFEIA 2073
            ||||..|||:.|  .::|:||.||.||.||||||.|||||.|.||.|....:.||....:.|:|.
 Frog  1315 DNFNQQKKKFGG--KDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPTNKIQGLVYDFVTKQLFDIV 1377

  Fly  2074 IFVLIFLNMLTMGIEHYDQPHAVFFILEVSNAFFTTVFGLEAIVKIVGLRYHYFTVPWNVFDFLL 2138
            |.:||.|||:||.||..||......||...|..|..:|..|.::|:..|||::||:.||:|||::
 Frog  1378 IMILICLNMVTMMIETDDQSQEKTDILFKINLVFIVIFTAECVLKMFALRYYFFTIGWNIFDFVV 1442

  Fly  2139 VLASIFGILMEDIMIDLPISPTLLRVVRVFRIGRILRLIKAAKGIRKLLFALVVSLPALFNIGAL 2203
            |:.||.||::.|::.:..:||||.||:|:.||||:||||:.|||||.|||||::|||||||||.|
 Frog  1443 VILSIVGIVLSDLIAEYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLL 1507

  Fly  2204 LGLITFIYAILGMSLFGNVKLQGALDDMVNFQTFGRSMQLLFRLMTSAGWNDVLESLMIQ-PPDC 2267
            |.|:.|||:|.|||.|..||.:..:||:.||:|||.|:..||::.|||||:.:|..::.. ||||
 Frog  1508 LFLVMFIYSIFGMSNFAYVKKESGIDDIFNFETFGNSIICLFQITTSAGWDGLLNPILNSGPPDC 1572

  Fly  2268 DPFIH--GHT-NGNCGHPLLAITYFTSFIIISYMIVINMYIAIILENFNQAHQEEEIGIVEDDLE 2329
            ||.:.  |.| .|:||:|.:.|.:|.|:||||::||:||||||||||||.|.:|....:.|||.|
 Frog  1573 DPHLENFGTTVTGDCGNPAIGIVFFCSYIIISFLIVVNMYIAIILENFNVATEESGEPLCEDDFE 1637

  Fly  2330 MFYIRWSKYDPHATQFIHFSQLSDFIASLDPPLGISKPNNVALVSFNLPISKGNKIHCLDILHAL 2394
            |||..|.|:||.||||:.:|::|||:.:|..||.|.|||.:.|::.:||:..|:||||||||.||
 Frog  1638 MFYETWEKFDPDATQFVEYSRMSDFVDTLQDPLRIPKPNKIKLITLDLPMVTGDKIHCLDILFAL 1702

  Fly  2395 VKHVLGHVEETDNFKQLQEQMDVKFKKQFPTRKELEIVSSTRIWKRQEKAAKTIQTGWKEYLRRK 2459
            .|.|||...|.|   .|:..|:.||....|::...|.:::|...|::|.....||..::.:|.|:
 Frog  1703 TKEVLGDSGEMD---ALKASMEEKFMMANPSKVSYEPITTTLKRKQEEVCVIKIQRAFRRHLLRR 1764

  Fly  2460 REKERS----NSGDSATQTSSP----GGWQSKLSALNFFHLQVSRRGTACSSRASSRKSSRASDA 2516
            ..|..|    .|.|..:....|    |....:::|....:::..:.|.....|:::|.|..:.|.
 Frog  1765 SVKHASYIFRQSNDEESSVEEPPEKEGLIAERINAAYGKYVEEGQNGGTHRPRSTNRTSHSSIDY 1829

  Fly  2517 SD 2518
            .|
 Frog  1830 DD 1831

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NaCP60E	NP_001261172.1	Ion_trans	125..371	CDD:459842	121/279 (43%)
		Ion_trans	687..902	CDD:459842	118/218 (54%)
		Ion_trans	1751..2019	CDD:459842	156/274 (57%)
		Na_channel_gate	2009..2063	CDD:240441	30/53 (57%)
		Ion_trans	2067..2320	CDD:459842	135/256 (53%)
		GPHH	2332..2378	CDD:465306	21/45 (47%)
scn4a	XP_017944811.2	Ion_trans	125..440	CDD:459842	142/314 (45%)
		Ion_trans	599..812	CDD:459842	116/215 (54%)
		Na_trans_assoc	838..1045	CDD:461936	74/822 (9%)
		Ion_trans	1049..1323	CDD:459842	154/273 (56%)
		Na_channel_gate	1315..1367	CDD:240441	30/53 (57%)
		Ion_trans	1372..1628	CDD:459842	135/255 (53%)