Protein Alignment mAChR-A and chrm5a

DIOPT Version :10

• Sequence 1: NP_523844.2
• Gene: mAChR-A / 37892
• FlyBaseID: FBgn0000037
• Length: 805
• Species: Drosophila melanogaster
• Sequence 2: NP_001018639.1
• Gene: chrm5a / 553978
• ZFINID: ZDB-GENE-080723-32
• Length: 490
• Species: Danio rerio
• Alignment Length: 715
• Identity: 222/715 - (31%)
• Similarity: 304/715 - (42%)
• Gaps: 277/715 - (38%)

  Fly    99 YSLASMVVMGFVAAILSTVTVAGNVMVMISFKIDKQLQTISNYFLFSLAIADFAIGAISMPLFAV 163
            :||..::.:..|:||:|.:|:.||::||:|||::.||:|::||:|.|||.||..||..||.|:..
Zfish    20 HSLWEVIAIATVSAIVSFITIIGNILVMLSFKVNSQLKTVNNYYLLSLAFADLIIGVFSMNLYTS 84

  Fly   164 TTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAA 228
            ..::|||.||.:.||.||||||:||||||:|||:||||||||:|||||||||||...|.:|||.|
Zfish    85 YILMGYWALGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTPKIAGIMIGLA 149

  Fly   229 WGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTIMCFLYWRIWR 293
            |.:|.:||.|.|..|.|..||||||:.:|.|||. :...||||||:|||||||::|..||..|::
Zfish   150 WLVSFILWAPPILCWQYFVGKRTVPERQCQIQFF-SEPVITFGTAIAAFYFPVSVMTILYCLIYK 213

  Fly   294 ETKKRQKDLPNLQAGKKDSSKRSNSSDENTVVNHASGGLLAFAQVGGNDHDTWRRPRSESSPDAE 358
            ||::|.|||..||.        .|||                               :.||.||:
Zfish   214 ETERRTKDLAELQG--------INSS-------------------------------TNSSGDAQ 239

  Fly   359 SVYMTNMVIDSGYHGMH----SRKSSIKSTN---TIKKSYTCFGSIKEWCIAWWHSGREDSD--- 413
            .     ..|.|.:...|    ||..::..||   |:....|.|.|         ::..|:.|
Zfish   240 P-----QKIRSCFGCKHVSNTSRTQALSHTNAAKTLDDQLTNFNS---------YASSEEEDRSG 290

  Fly   414 ---DFAYEQEEPSDLGYATPVTIETPLQSS---VSRCTSMNVMRDNYSMGGSVSGVRPPSILLSD 472
               :....||..|: .|.......||:::|   ..:|.|..              .:|     .|
Zfish   291 NFQESCRHQENKSE-SYEEENFFPTPVKASPTKTKKCVSYK--------------FKP-----KD 335

  Fly   473 VSPTPLPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNNNASHNGNGAVNGNGAGNGSG 537
            |||                                     :.|.|
Zfish   336 VSP-------------------------------------LKNTN-------------------- 343

  Fly   538 IGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASSNAANGGGGGPGAGAAASA 602
                                                                |...|||.:.:||
Zfish   344 ----------------------------------------------------GDAKPGASSFSSA 356

  Fly   603 SLSMQGDCAPSIKMIHEDGPTTTAAAAPLASAAATRRPLPSRDSEFSLPLGRRMSHAQHDARLLN 667
            .         |:     :.|::::::.|:...                               |.
Zfish   357 E---------SV-----NAPSSSSSSKPIDGT-------------------------------LK 376

  Fly   668 AKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQESKAAKTLSAILLSFIITWTP 732
            .::                               .|:|.....:|.|||:|||||||:||:||||
Zfish   377 CQI-------------------------------TKRKRMVLIKEKKAAQTLSAILLAFILTWTP 410

  Fly   733 YNILVLIKPLTTCSDCIPTELWDFFYALCYINSTINPMCYALCNATFRRTYVRILTCKWHTRNRE 797
            |||:|||.  |.||||||..||...|.|||:|||:|||||||||.||::|:..:|.|:|..:..|
Zfish   411 YNIMVLIS--TFCSDCIPLSLWHLGYWLCYVNSTVNPMCYALCNKTFQKTFRMLLLCQWRKQRAE 473

  Fly   798  797
            Zfish   474  473

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
mAChR-A	NP_523844.2	7tmA_mAChR_DM1-like	105..>300	CDD:320428	112/194 (58%)
		TM helix 1	106..132	CDD:320428	12/25 (48%)
		TM helix 2	139..165	CDD:320428	13/25 (52%)
		TM helix 3	177..207	CDD:320428	25/29 (86%)
		TM helix 4	219..242	CDD:320428	11/22 (50%)
		TM helix 5	265..294	CDD:320428	17/28 (61%)
		7tm_GPCRs	<707..783	CDD:475119	50/75 (67%)
		TM helix 6	716..738	CDD:410628	17/21 (81%)
		TM helix 7	751..776	CDD:410628	16/24 (67%)
chrm5a	NP_001018639.1	7tmA_mAChR_M5	26..459	CDD:320427	215/693 (31%)
		TM helix 1	27..53	CDD:320427	12/25 (48%)
		TM helix 2	60..86	CDD:320427	13/25 (52%)
		TM helix 3	98..128	CDD:320427	25/29 (86%)
		TM helix 4	140..163	CDD:320427	11/22 (50%)
		TM helix 5	185..214	CDD:320427	17/28 (61%)
		TM helix 6	388..418	CDD:320427	22/29 (76%)
		TM helix 7	427..452	CDD:320427	16/24 (67%)