DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mAChR-A and chrm5

DIOPT Version :9

Sequence 1:	NP_523844.2	Gene:	mAChR-A / 37892	FlyBaseID:	FBgn0000037	Length:	805	Species:	Drosophila melanogaster
Sequence 2:	XP_017952476.1	Gene:	chrm5 / 100498518	XenbaseID:	XB-GENE-986590	Length:	525	Species:	Xenopus tropicalis

Alignment Length:	730	Identity:	230/730 - (31%)
Similarity:	314/730 - (43%)	Gaps:	246/730 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly    84 NQTNGTIGFETKGPRYSLASMVVMGFVAAILSTVTVAGNVMVMISFKIDKQLQTISNYFLFSLAI 148
            |.:|||:..:.    :||..::.:..|:||:|.:|:.||::||:|||::.||:|::||:|.|||.
Frog     9 NNSNGTVPLKA----HSLWEVITIATVSAIVSLITIVGNILVMVSFKVNSQLKTVNNYYLLSLAC 69

  Fly   149 ADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYR 213
            ||..||..||.|:....::|:|.||.:.||.||||||:||||||:|||:||||||||:|||||||
Frog    70 ADLIIGIFSMNLYTSYILIGHWSLGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYR 134

  Fly   214 AKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFY 278
            ||||..||.:|||.||.||.:||.|.|..|.|..|.||||.:||.|||: ....||||||:||||
Frog   135 AKRTPKRAGLMIGLAWFISFILWAPAILCWQYFVGGRTVPPEECQIQFL-YEPIITFGTAIAAFY 198

  Fly   279 FPVTIMCFLYWRIWRETKKRQKDLPNLQAGKKDSSKRSNSSDENTVVNHASGGLLAFAQVGGNDH 343
            .||::|..||.||::||:||.|||..||.
Frog   199 LPVSVMTILYCRIYKETEKRTKDLAELQG------------------------------------ 227

  Fly   344 DTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKSYTCFG------SIKEWCI 402
                   |||..|.|                     .::...|:.||  ||.      :.:|.|.
Frog   228 -------SESVADFE---------------------MMRPQGTLFKS--CFSCKQQTRTKRERCQ 262

  Fly   403 AWWHSGREDSDDFAYEQEEPSDLGYATPVTIETPLQSSVSRCTSMNVMRDNYSMGGSVSGVRPPS 467
            |.|                                 ||.||.|:..|
Frog   263 ASW---------------------------------SSSSRSTTTTV------------------ 276

  Fly   468 ILLSDVSPTPLPRPPLASISQLQEMSAVTASTTANVNT-SGNGNGAINNNNNASHNGNGAVNGNG 531
                .::.:|..........||...|:..:|...:..| .....||..|...|
Frog   277 ----KITHSPNTCNEWIKEDQLTTCSSYPSSDDEDKQTKEAVFQGAYENQTEA------------ 325

  Fly   532 AGNGSGIGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASSNAANGGGGGPGA 596
                            .::..|..::.:......:.:|....|.                  ||.
Frog   326 ----------------QKEEETKQILTKEQPGLSNYESERFFLT------------------PGK 356

  Fly   597 GAAASASLSMQGDCAPSIK---MIHEDGPTTT------AAAAPLASAAATRRPLPSRDSEFSLPL 652
            |.      |.:.....|.|   ::::||....      ....|.:|
Frog   357 GH------SQKSPKCVSYKFRIVVNDDGSQEVNNGCRKVKITPCSS------------------- 396

  Fly   653 GRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQESKAAK 717
               ||.. |..|.::..:                   :|.:        .|:|.....:|.|||:
Frog   397 ---MSKG-HSLRSMDPSI-------------------SHQI--------TKRKRMVLIKERKAAQ 430

  Fly   718 TLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTINPMCYALCNATFRRT 782
            |||||||:||||||||||:||:.  |.||||||:.||...|.|||:|||:||:||||||.|||:|
Frog   431 TLSAILLAFIITWTPYNIMVLVS--TFCSDCIPSSLWHLGYWLCYVNSTVNPICYALCNKTFRKT 493

  Fly   783 YVRILTCKWHTRNRE 797
            :..:|.|:|..:..|
Frog   494 FKMLLLCRWRKKTVE 508

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mAChR-A	NP_523844.2	7tm_4	117..>241	CDD:304433	76/123 (62%)
mAChR-A	NP_523844.2	7tm_1	121..>314	CDD:278431	114/192 (59%)
chrm5	XP_017952476.1	7tm_GPCRs	26..>220	CDD:391938	114/194 (59%)
		TM helix 1	28..52	CDD:341315	11/23 (48%)
		TM helix 2	61..83	CDD:341315	13/21 (62%)
		TM helix 3	99..121	CDD:341315	17/21 (81%)
		TM helix 4	144..160	CDD:341315	9/15 (60%)
		TM helix 5	186..209	CDD:341315	14/22 (64%)
		7tm_GPCRs	<423..502	CDD:391938	53/80 (66%)
		TM helix 6	426..451	CDD:341315	20/24 (83%)
		TM helix 7	462..487	CDD:341315	15/24 (63%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	352	1.000	Domainoid score	I1000
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	393	1.000	Inparanoid score	I1932
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1245472at2759
OrthoFinder	1	1.000	-	-		FOG0001308
OrthoInspector	1	1.000	-	-		otm48994
Panther	1	1.100	-	-	O	PTHR24247
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2382
SonicParanoid	1	1.000	-	-		X324
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	10	10.100