Protein Alignment mAChR-A and chrm5

DIOPT Version :10

• Sequence 1: NP_523844.2
• Gene: mAChR-A / 37892
• FlyBaseID: FBgn0000037
• Length: 805
• Species: Drosophila melanogaster
• Sequence 2: XP_017952476.1
• Gene: chrm5 / 100498518
• XenbaseID: XB-GENE-986590
• Length: 525
• Species: Xenopus tropicalis
• Alignment Length: 730
• Identity: 230/730 - (31%)
• Similarity: 314/730 - (43%)
• Gaps: 246/730 - (33%)

  Fly    84 NQTNGTIGFETKGPRYSLASMVVMGFVAAILSTVTVAGNVMVMISFKIDKQLQTISNYFLFSLAI 148
            |.:|||:..:.    :||..::.:..|:||:|.:|:.||::||:|||::.||:|::||:|.|||.
 Frog     9 NNSNGTVPLKA----HSLWEVITIATVSAIVSLITIVGNILVMVSFKVNSQLKTVNNYYLLSLAC 69

  Fly   149 ADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYR 213
            ||..||..||.|:....::|:|.||.:.||.||||||:||||||:|||:||||||||:|||||||
 Frog    70 ADLIIGIFSMNLYTSYILIGHWSLGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYR 134

  Fly   214 AKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFY 278
            ||||..||.:|||.||.||.:||.|.|..|.|..|.||||.:||.|||: ....||||||:||||
 Frog   135 AKRTPKRAGLMIGLAWFISFILWAPAILCWQYFVGGRTVPPEECQIQFL-YEPIITFGTAIAAFY 198

  Fly   279 FPVTIMCFLYWRIWRETKKRQKDLPNLQAGKKDSSKRSNSSDENTVVNHASGGLLAFAQVGGNDH 343
            .||::|..||.||::||:||.|||..||.
 Frog   199 LPVSVMTILYCRIYKETEKRTKDLAELQG------------------------------------ 227

  Fly   344 DTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKSYTCFG------SIKEWCI 402
                   |||..|.|                     .::...|:.||  ||.      :.:|.|.
 Frog   228 -------SESVADFE---------------------MMRPQGTLFKS--CFSCKQQTRTKRERCQ 262

  Fly   403 AWWHSGREDSDDFAYEQEEPSDLGYATPVTIETPLQSSVSRCTSMNVMRDNYSMGGSVSGVRPPS 467
            |.|                                 ||.||.|:..|
 Frog   263 ASW---------------------------------SSSSRSTTTTV------------------ 276

  Fly   468 ILLSDVSPTPLPRPPLASISQLQEMSAVTASTTANVNT-SGNGNGAINNNNNASHNGNGAVNGNG 531
                .::.:|..........||...|:..:|...:..| .....||..|...|
 Frog   277 ----KITHSPNTCNEWIKEDQLTTCSSYPSSDDEDKQTKEAVFQGAYENQTEA------------ 325

  Fly   532 AGNGSGIGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASSNAANGGGGGPGA 596
                            .::..|..::.:......:.:|....|.                  ||.
 Frog   326 ----------------QKEEETKQILTKEQPGLSNYESERFFLT------------------PGK 356

  Fly   597 GAAASASLSMQGDCAPSIK---MIHEDGPTTT------AAAAPLASAAATRRPLPSRDSEFSLPL 652
            |.      |.:.....|.|   ::::||....      ....|.:|
 Frog   357 GH------SQKSPKCVSYKFRIVVNDDGSQEVNNGCRKVKITPCSS------------------- 396

  Fly   653 GRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQESKAAK 717
               ||.. |..|.::..:                   :|.:        .|:|.....:|.|||:
 Frog   397 ---MSKG-HSLRSMDPSI-------------------SHQI--------TKRKRMVLIKERKAAQ 430

  Fly   718 TLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTINPMCYALCNATFRRT 782
            |||||||:||||||||||:||:.  |.||||||:.||...|.|||:|||:||:||||||.|||:|
 Frog   431 TLSAILLAFIITWTPYNIMVLVS--TFCSDCIPSSLWHLGYWLCYVNSTVNPICYALCNKTFRKT 493

  Fly   783 YVRILTCKWHTRNRE 797
            :..:|.|:|..:..|
 Frog   494 FKMLLLCRWRKKTVE 508

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
mAChR-A	NP_523844.2	7tmA_mAChR_DM1-like	105..>300	CDD:320428	114/194 (59%)
		TM helix 1	106..132	CDD:320428	12/25 (48%)
		TM helix 2	139..165	CDD:320428	13/25 (52%)
		TM helix 3	177..207	CDD:320428	25/29 (86%)
		TM helix 4	219..242	CDD:320428	13/22 (59%)
		TM helix 5	265..294	CDD:320428	17/28 (61%)
		7tm_GPCRs	<707..783	CDD:475119	50/75 (67%)
		TM helix 6	716..738	CDD:410628	18/21 (86%)
		TM helix 7	751..776	CDD:410628	15/24 (63%)
chrm5	XP_017952476.1	7tm_GPCRs	26..>220	CDD:475119	114/194 (59%)
		TM helix 1	28..52	CDD:320427	11/23 (48%)
		TM helix 2	61..83	CDD:320427	13/21 (62%)
		TM helix 3	99..121	CDD:320427	17/21 (81%)
		TM helix 4	144..160	CDD:320427	9/15 (60%)
		TM helix 5	186..209	CDD:320427	14/22 (64%)
		7tm_GPCRs	<423..502	CDD:475119	53/80 (66%)
		TM helix 6	429..451	CDD:320426	18/21 (86%)
		TM helix 7	462..487	CDD:320426	15/24 (63%)