Protein Alignment Golgin245 and Cenpf

DIOPT Version :10

• Sequence 1: NP_611787.2
• Gene: Golgin245 / 37702
• FlyBaseID: FBgn0034854
• Length: 1489
• Species: Drosophila melanogaster
• Sequence 2: NP_001074832.2
• Gene: Cenpf / 108000
• MGIID: 1313302
• Length: 2997
• Species: Mus musculus
• Alignment Length: 1774
• Identity: 382/1774 - (21%)
• Similarity: 697/1774 - (39%)
• Gaps: 467/1774 - (26%)

  Fly     4 NLKNKLIEEVKASPSKFQQ-------FAN------------AAQAAVSSSSSTTPNSETNTSN-N 48
            |.||:| |::|.:.:|.||       ||.            ..|...|..::|..:|.:.|.. .
Mouse  1072 NRKNEL-EQLKETFAKEQQEFLTKLAFAEEQNRKLMLELEIEQQTVRSEITNTNKHSMSATDGLR 1135

  Fly    49 ENFFSITEEDTPQNSPYRIQKLPATSASALRGRSTQSLNGATSRTRKLSNSSMASDVSFRLPTYE 113
            :...::.||...|.:                     .::..|....:|...:.....|:.
Mouse  1136 QECLTLNEEQNEQQN---------------------EVSNLTHENEQLMELTQTKHDSYL----- 1174

  Fly   114 APAVYHLQSDLDETSSEFDDSASTARLDVITKDQLYDAYKK--------------SLDRYHKYRC 164
              ||..:::.:..|..|...|:|..::|:.|||...|:||.              .||:..:...
Mouse  1175 --AVEPVENSVKATEDEIGKSSSQYQMDIDTKDISLDSYKAQLVHLEALVRILEVQLDQSEEENK 1237

  Fly   165 R-YTDLAKKYKELERDS-----SKARSVLVETQDKALRRISELREQCTLEQQAKAHLEEALRVEM 223
            : :.:|....:|||..|     |:||:.|.:. |.|..:...:.::.:..|...|||:
Mouse  1238 KLHLELQTIREELETKSSQDPQSQARTGLKDC-DTAEEKYVSMLQELSASQNENAHLQ------- 1294

  Fly   224 DDMSCKMQAYQTKLQLLGENPENITAALERSGQQLESEQLIDLEESIGKSPLSTNGSSGVSDLQR 288
                |.:|....||..||:    :...|.....|||||    |.:|  ::...|..|...:::::
Mouse  1295 ----CSLQTAVNKLNELGK----MCDVLRVEKLQLESE----LNDS--RTECITATSQMTAEVEK 1345

  Fly   289 LLKE-----------RDEQLKSVTEKYEAVRKQEEENVLLLAQTKQAIHTELELKDTEVR----K 338
            |:.|           ::|.:|. |...|...|....:|.|..........::.|...|||    :
Mouse  1346 LVSEMKMLNHESALSQNELMKD-TSGGEFHDKANHSSVFLTPLDSSNFCEQMTLSSKEVRVHFAE 1409

  Fly   339 LQEKLKQLESQRESHNNEVKEQFKKLQATKQEVDAKLMATEHLLNTLKESYAIKEQQVVTLEAQL 403
            ||||...|:|:.           |.|.....||.:|:.|....::|||...:.....:.||:..|
Mouse  1410 LQEKFSCLQSEH-----------KILHDQHCEVSSKMSALRSYVDTLKAENSALSMSLRTLQGDL 1463

  Fly   404 EAIRVENEQKV---------------------------KDLQKQNEDR-------NTQA------ 428
            ..   |.|...                           ||:.:|..|.       |..|
Mouse  1464 VK---EGEPAAEGGHGLPLSFCGADSPSLTNFGETSFYKDVLEQTGDTCHLSLEGNASANSCDLD 1525

  Fly   429 ---SDSSEQ--------------------------LKKLQAAVQDAESQLLSKDQLLESLRSEQA 464
               |.|.|:                          |:.|:...:..|||.:.|::.:|.|....:
Mouse  1526 EEFSSSLEEETLTEKESPPAPGRTVEGLEVLCQVYLQSLKNLEEKTESQRIMKNKEIEKLEQLLS 1590

  Fly   465 AKEQQLKHLKEQLGKLKQENENYLDKLRENKKSSDSQTNEAQDQQK----KLQAAK------DEA 519
            ::.::|..|::|   ...|.|.:..||.......:|:..|.:.|.|    :|:.|:      |.:
Mouse  1591 SERKELSCLRKQ---YLSEKEQWQQKLTSVTLEMESKLAEEKQQTKTLSLELEVARLQLQELDLS 1652

  Fly   520 ESKLLAT--EELLHSLRNDYKAQEEKVALLEDKLKTLSKENDVNVEKLHHINEQREAQSTDSQQK 582
            ...||.|  |.::....::|..:|.:|.:.|...||..::.|...:|             |.||.
Mouse  1653 SRSLLGTDLESVVRCQNDNYDIKESEVYISETTEKTPKQDTDQTCDK-------------DIQQD 1704

  Fly   583 INELRAAKDEAEAKLLSTE------HSLNALQAALSAK----EEQAASLEQSLNAL----KTESE 633
            :. |..:..|:|...|:.|      ...|. :|....|    .|..:.|..|.|.|    ..|.:
Mouse  1705 LG-LETSVTESETTRLTGEGCEEQPPKTNC-EAPAEDKTQDCSECISELCSSSNVLVPMDVLEDQ 1767

  Fly   634 HSLQDLRLHNDQLLEIVQ-RHQQNDWEAQLARAREELAAIQSQRELHALELEKS----LEMERES 693
            .|:|:|:|..|.|.|.:: ..:..||:.::.....|:....|:..|..::|:..    :::|: .
Mouse  1768 GSIQNLQLQKDTLNENLRLLPEVEDWDKKVESLLNEIMEADSKLSLQEVQLKMKIATCIQLEK-I 1831

  Fly   694 VAALNSEKASQEEQHR---------LKLEQLQREIQILQDQHANSESETVAALKGQLEALSQDLA 749
            |..|..|||...|:..         |::|:.:.::....|..||.          .|...::|.|
Mouse  1832 VKDLRKEKADLSEKLESLPCNQEVCLRVERSEEDLGFNLDMGANE----------LLSKSTKDNA 1886

  Fly   750 TSQASLLAKEKELKASGNKLNKIK--KQHEQHQAKSSDQSARLEALQSELADRLSHSRQVESEKE 812
            |:..... |||.|... .:|.:||  |.:.:|...|.:  ..||.:|   |::|...|..||:::
Mouse  1887 TNTEDNY-KEKFLDME-RELTRIKSEKANIEHHILSVE--TNLEVVQ---AEKLCLERDTESKQK 1944

  Fly   813 ---ELQARVTGILEEIGTMQAQMQQVQDSHSELEREKRKLESRIESLQQEQVDS----SAQDERT 870
               :|:..:..::.|...::.::..|......|::..:|::.:||.|:..|.:|    .|.:...
Mouse  1945 VIIDLKEELFTVISERNRLREELDNVSKESKALDQMSKKMKEKIEELESHQRESLRHIGAVESEV 2009

  Fly   871 SAKLEEIQSENTKLAERNCLLEEQTNHLESQLQAKQDE-------IGKIQAKLQQVLDEHSKLQN 928
            ..|.:.||:.:..:.|    |.:...||:.|||..|::       ||:::.::.|:..|...|
Mouse  2010 KDKADLIQTLSFNVGE----LTKDKAHLQEQLQNLQNDSQELSLAIGELEIQIGQLNKEKESL-- 2068

  Fly   929 AQELMDHDHRTLQDKCDAY-----------EKDKLLTKHTLDCLQSASEELHRVKANLDR----- 977
            .:|..:...:..:.:|:..           ||.:...:     |.||.||:|:::..:::
Mouse  2069 VKESQNFQIKLTESECEKQTISKALEVALKEKGEFAVQ-----LSSAQEEVHQLRRGIEKLSVRI 2128

  Fly   978 --ELKEQDQQLSELRERQREQEQQLKDQAERCAKLKAQNSESE-------TQLQATISNLREQLD 1033
              :.|:....:::|:|.|||.: .|||..|...:...::.|::       ..|:|.:..|:.|.|
Mouse  2129 EADEKKHLSAVAKLKESQRESD-SLKDTVETLERELERSEENQELAILDSENLKAEVETLKAQKD 2192

  Fly  1034 --------------AYKQTEQGIQEKLQATNSSYTTQIATLEARWSAANSDVERLHEANDALQLE 1084
                          ..:...:.:.::||...|    :::.|:.|.|:    :.||.|..:..:::
Mouse  2193 EMTKSLRIFELDLVTVRTERENLAKQLQEKQS----RVSELDERCSS----LRRLLEEKEQARVQ 2249

  Fly  1085 MEQ--------LKIKHGQEREEV-------------KESIAQKNRQVVELQEAMATRDRQLQEKI 1128
            ||:        |:::..:.||||             ::|:.|...:|..|:.::    |:|:..|
Mouse  2250 MEEDSKSAMLMLQMQLKELREEVAALCNDQETLKAQEQSLDQPGEEVHHLKSSI----RKLKVHI 2310

  Fly  1129 EASEKLAKFDEILIENEYLNKHTKQLEAELAESAE----LKEKLKSLQCELYVLQEKAEQHAVQM 1189
            :|.||               ||...|| :|.||..    ||:::::|:.|| :|.||   :.:..
Mouse  2311 DADEK---------------KHQNILE-QLKESKHHADLLKDRVENLEQEL-ILSEK---NMIFQ 2355

  Fly  1190 AEKETQSATATAEVSELKKAIEEQAVELTRQKEHASFVTEQSDAVQKDLLQAQQQ---------- 1244
            |||      :.||:..||..|:..|..|...:........:::.:.|:|.:.|::
Mouse  2356 AEK------SKAEIQTLKSEIQRMAQNLQDLQLELISTRSENENLMKELKKEQERVSDLETINSS 2414

  Fly  1245 ----LHDKQIELAMSRDEQ----ALLQAEADGLRQEVI--CLKEHLSPSTDSDSLRSLNERLQRE 1299
                |.||:.|....::|.    .:||.:...|.:.|:  |..:.:| .|...:|.|..:.|:.|
Mouse  2415 IENLLKDKEQEKVQMKEEAKITVEMLQTQLKELNETVVSLCNDQEVS-KTKEQNLGSQVQTLELE 2478

  Fly  1300 ----LEDL---KHKSAGAESNMQQEIEELQANNQQMAERINELETLRAGIQAQ-QLLASMAPKNV 1356
                |:||   |:|....:|::....:|::|..|::.:...|:.||:..:::| ||:..:|....
Mouse  2479 KAQLLQDLGEAKNKYIIFQSSVNALTQEVEAGKQKLEKGEKEIRTLKEQLKSQEQLVCKLAQVEG 2543

  Fly  1357 QEAAAAGEKAELESKLKEIMNEVQDVTNRNLFLEQKCENFLILEQSNERLK-----LQNAKLSRQ 1416
            ::.....:|.||.              |..:.||||.:   :|:..|..|:     |||:..|.:
Mouse  2544 EQQLWQKQKLELR--------------NVTMALEQKVQ---VLQSENNTLQSTYEALQNSHKSLE 2591

  Fly  1417 LDETLVSMQHSEAVP-----ANTEFEYLRNI--MFQYLTG-----NTNNETLVKVISAVLKFSPQ 1469
            .:..|:.::....|.     :..|.|..|.:  |.|..|.     |.....|.:.:. ||:...|
Mouse  2592 SELGLIKLEKVALVERVSTISGKEAELQRELRDMLQKTTQLSEDYNKEKNRLTEEVE-VLREELQ 2655

  Fly  1470 QAQVA-------LEKEHQR 1481
            ..:.|       ||||.||
Mouse  2656 NTKAAHLKSVNQLEKELQR 2674

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Golgin245	NP_611787.2	Smc	286..>856	CDD:440809	147/698 (21%)
		Smc	583..1247	CDD:440809	169/785 (22%)
		MIT_CorA-like	<1244..>1333	CDD:469776	25/115 (22%)
		Grip	1433..1477	CDD:197860	13/57 (23%)
Cenpf	NP_001074832.2	CENP-F_N	1..300	CDD:463106
		SMC_prok_B	290..1122	CDD:274008	13/50 (26%)
		SMC_prok_B	<1550..2248	CDD:274008	160/753 (21%)
		SMC_prok_B	<1959..2746	CDD:274008	173/785 (22%)
		CENP-F_leu_zip	2131..2270	CDD:463102	28/147 (19%)
		CENP-F_leu_zip	2313..2449	CDD:463102	38/161 (24%)
		CENP-F_C_Rb_bdg	2850..2896	CDD:463111