Protein Alignment Isha and scaf4

DIOPT Version :10

• Sequence 1: NP_001097394.1
• Gene: Isha / 37411
• FlyBaseID: FBgn0034598
• Length: 1306
• Species: Drosophila melanogaster
• Sequence 2: XP_012813473.1
• Gene: scaf4 / 595037
• XenbaseID: XB-GENE-920206
• Length: 1340
• Species: Xenopus tropicalis
• Alignment Length: 1499
• Identity: 458/1499 - (30%)
• Similarity: 616/1499 - (41%)
• Gaps: 400/1499 - (26%)

  Fly     1 METVVAFNNELSGLYDSRPPISKAKMAAITKSAMRAIKLYKHVVQSVEKFILKCKPEYKVPGLYV 65
            |:.|.|||.||..|.|.:||||:|||..|||:|::||||||||||.|||||.|||||||||||||
 Frog     1 MDAVNAFNQELFSLMDMKPPISRAKMILITKAAIKAIKLYKHVVQIVEKFIKKCKPEYKVPGLYV 65

  Fly    66 IDSIVRQSRHQYGMDKDLFAPRFQRNLTETFANLFRCAPEDKSRIIRVLNLWQKNNVFKSEVIQP 130
            |||||||||||:|:|||:|.|||.:|||.||..|:.|..||||:|:|||||||||.|||.|:|||
 Frog    66 IDSIVRQSRHQFGIDKDVFGPRFTKNLTATFQCLYTCPSEDKSKIVRVLNLWQKNGVFKIEIIQP 130

  Fly   131 IFDLADPNHPIYHQMPPVGGSGGQGGGV---------GPGPSSSGALS-----------LADISS 175
            :.|:|               :|.....|         |..|.|...||           :|.:..
 Frog   131 LLDMA---------------AGAAAADVMTDSSMMEEGSSPPSGMILSEPPDEVQEPPPVAPVPQ 180

  Fly   176 GPNGLNSSGMEISMNSSGGDDKMGGAMPDLSLGHEKFGGGSSSSKRHYSEQHYSKRQSVVSCSSK 240
            .|.....:.:.....|:.|.        .|....:.|............|..:::.|::.|..:.
 Frog   181 LPTPDAFAAVAQLFQSTQGQ--------QLQQILQTFQQPPKPQSPAIDENVFAQVQAITSQLNA 237

  Fly   241 TSKSHHHKREYVKSSHDIDYQVREIIEEEDEGLQMLMADDHHCMGDDSPPTSSNLLDEKKIKQLL 305
            .:.....|..:.|.....||.     :|.|       |.:.......|||.|.:...:..::...
 Frog   238 PAHQPERKTAFDKLLDRFDYD-----DEPD-------ATEESKKEASSPPLSGSQQSQPPLQPFA 290

  Fly   306 NNPNVLR----------QLHTLQNFQNL---KPQEENQKHRYQDEALQQHFQNVMKGN------- 350
            .:.:.::          |:..:..||.:   .||          |.|.|..|....|.
 Frog   291 FSADSMQPAAYPGQLPGQVPNMDQFQPMMMGMPQ----------EPLPQQTQLPPNGQMLGFGMV 345

  Fly   351 -----AGMPPGMG-----------MGMNMNDSMDLNKDVEFISEQQTIEVINLDGGDSRSPTPDR 399
                 |.|.|.||           ..|..|: |.:.|:     .||.:.:      |...|....
 Frog   346 PTQSFAPMVPPMGSEPSTHGFQNTFQMQPNE-MHVQKE-----HQQEMPM------DVEQPNVQE 398

  Fly   400 DRYKRSRRNSRSRTRSPRGRGAGGGTGNDRRRRGTRSRSRSRSPRSSRRRGSRDRDRMDRSNRDK 464
            :: :.....|:|.:|||:.|         |.|.|:||| |||..||..|  ||||.|....:|.:
 Frog   399 NK-RHMNEQSKSVSRSPKRR---------RSRSGSRSR-RSRHRRSRSR--SRDRRRHSPKSRSQ 450

  Fly   465 E-RDREHERERRKKGLPDIKKEHLSVCSTTLWVGHLSKLVYQEELSDTFGEYGDIVSIDQIVPRG 528
            | ||||.|:|||:||||.||.|.:|:||||||:|.|.|...|:::.....|:|.|.||:.|.|||
 Frog   451 ERRDREKEKERRQKGLPPIKSETVSICSTTLWIGQLDKKTTQQDVGSFLEEFGPIESINMIPPRG 515

  Fly   529 CAFIVMNRRQDAHKAMQALK--NHKLQGRAITISWAAGKGVKSKEWKDYWDLELGVTYIPWSKLS 591
            ||:|||..||||::|:..|.  |.|:..:||.|:||..||:|: |:|.|||:|:|||||||:|:.
 Frog   516 CAYIVMVHRQDAYRALMKLSRGNVKVNQKAIKIAWALNKGIKA-EYKQYWDVEIGVTYIPWNKVK 579

  Fly   592 SDTDFDALEEGGMFDEDTM-----PIQMK-QKINQAKNAAKEHKGASIAEAAGAPSV----GVPP 646
            .: |.:...||||.|.||:     |:..| :..:.::|.|.|.||  :.||:.||.|    .:|.
 Frog   580 PE-DLENFCEGGMLDNDTLCPEWKPLAKKPEPKDVSQNGAAETKG--VGEASPAPQVLPLLSIPI 641

  Fly   647 PNLIFGIDTTQPPPVGPVG-----PVGPPPG----PGAPPPGLMGMVRGQFPMAPPMGINMPPPI 702
            ...|.|:   |.||:.|:.     |..|.||    .|.|||..:..::|    .||:. .||.|.
 Frog   642 RAPIPGM---QVPPLQPMTAHQPVPSIPTPGVPTVQGLPPPQGLPPLQG----VPPLQ-GMPLPQ 698

  Fly   703 MMPPTNMPPPMMMP-TTNMPP-----------PMMMPPTMMPPGF-PGIGGPP------HPMALP 748
            .:||..:|.|..|| ...|||           |...|..:.|||| ||:..||      :||.:|
 Frog   699 GLPPQGLPLPQGMPLPQGMPPHQPIPGMGPLQPPAFPIGIPPPGFAPGVPPPPFMRPGFNPMHMP 763

  Fly   749 PGAPFPPPGAVPP-------PIPSGGQISNSGNVSDDQMDIEMDLEDAPPLP---------PQQQ 797
            ||. .||||..||       |:|:.|....:........|...|:....|.|         ..::
 Frog   764 PGF-LPPPGLPPPMTPPVSIPLPTPGINIPTSTADTSSNDSSKDVSRTNPSPMVPVRDNMEEDEK 827

  Fly   798 AN----FNPSPSNVDLSPAAMANEMFQQRDRERDRDNRDRSRG--PG-------NSRWGGRDDVV 849
            |:    :.|:||....:.....|.:...:..|:.. |..:::|  ||       ....|.|....
 Frog   828 ADNGNGYKPTPSGGPNNAPGPLNPLHGDQQGEQGL-NSGQAQGAHPGPPQGPFSGQPQGPRHGTT 891

  Fly   850 EAAERWRAENG----------------------GGGGGPGSG----------LGPNAAFNEARAR 882
            |.|.:...|..                      |...||..|          |||...     .|
 Frog   892 EGAHQGPQEGSHQGPPEDTDHSPPGGRHYTLPEGPHHGPSEGHHHSPSEGPHLGPTEG-----PR 951

  Fly   883 LNLNPIEHGMPR--------------PDFMDFDNRGGPGGPRGMGPRGNHNG------------- 920
            |......||.|.              |:.:   :.|.|.||....|.|.::|
 Frog   952 LGPEGPHHGPPEGSHHGPQEHPHHGPPEGL---HHGPPEGPHHGTPEGQYHGPPEGPYHGPPEGP 1013

  Fly   921 --GGPGGDFF-PPNMNHNRFNQPTSLMQMRIPPPASFNQRMGGPAG-----NGGNGVGPMFMRNQ 977
              |.|.|.|. ||   |..::.         ||...|:   |.|.|     :.|...||.....|
 Frog  1014 YHGPPDGPFHGPP---HGPYHG---------PPQGPFH---GPPQGPYQGPHHGPPQGPHHGPPQ 1063

  Fly   978 G---GGPGG------AGPSGGGPGGAGTG-----------GGGPG--GRQQGPG--------FFN 1012
            |   |.|.|      .||..|.|.|.|:|           |..||  ..|:.||        |..
 Frog  1064 GPHHGPPQGPHHGPPQGPHHGPPQGPGSGPHPGPPSTGLLGARPGLIPLQRPPGIPSSNMQRFPM 1128

  Fly  1013 PRNPFNDNQRGRG---GQSG-----GGVRGVGGGGGGGPGGRGRWSDDEDEGGNNFKRRGGPGGP 1069
            .:.|...:...||   ||.|     .|:||...|.|..|..||      .||      .|||...
 Frog  1129 SQRPGPPHMMNRGPPPGQGGFGMPPHGIRGPLLGQGHFPRQRG------PEG------HGGPEER 1181

  Fly  1070 GGNRFRGER-----GGEMMDDRRGNNSRGGRGGPREDRERPGFGNRRGSRDDSNRHSISSTDEGN 1129
            .|.:|||||     |.|  .||.|..|.|     |.:.||..||.|   .||.:.
 Frog  1182 DGRQFRGERPVFPHGRE--PDRFGRRSFG-----RNEDERERFGRR---FDDPDH---------- 1226

  Fly  1130 KAASETEPKPKNVETELGSTNTGAPAAATRVDTEE--DWDQELQDYEARMEAQKPGERASQSANS 1192
                  |..|....:..|   .|:|:...:.|:||  .|....:     .|...|..|.....|.
 Frog  1227 ------ERPPSRGRSSWG---RGSPSRDRQRDSEERNRWPMGHE-----RERGHPRGRGDFLENR 1277

  Fly  1193 PPQEKLPAENENFAKPADVTGDPLVAKSQTETSAACTPLYDELPPPAVPQTP---TQPPKEPQGS 1254
            .|..:|...::.|:...      ..|::.||..........:.||...|:||   .:..|:|..:
 Frog  1278 KPDAELDENHKPFSSRM------RNAETVTERKEGKAEPAGKNPPEEQPETPKSSEEAEKDPGSN 1336

  Fly  1255 PPRP 1258
            |.:|
 Frog  1337 PAKP 1340

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Isha	NP_001097394.1	PHA03247	<1187..1306	CDD:223021	16/75 (21%)
		CID_SCAF8_like	6..135	CDD:340780	94/128 (73%)
		RRM_SCAF4_SCAF8	490..566	CDD:409674	37/77 (48%)
scaf4	XP_012813473.1	CID_SFRS15_SCAF4	5..135	CDD:340802	94/129 (73%)
		RRM_SF	475..555	CDD:473069	38/79 (48%)
		PHA03247	<603..907	CDD:223021	85/315 (27%)
		dnaA	873..>1044	CDD:455861	38/193 (20%)
		SF-CC1	1208..>1332	CDD:273721	33/161 (20%)