Protein Alignment Isha and scaf8

DIOPT Version :10

• Sequence 1: NP_001097394.1
• Gene: Isha / 37411
• FlyBaseID: FBgn0034598
• Length: 1306
• Species: Drosophila melanogaster
• Sequence 2: NP_001423676.1
• Gene: scaf8 / 326092
• ZFINID: ZDB-GENE-030131-3703
• Length: 1286
• Species: Danio rerio
• Alignment Length: 1506
• Identity: 447/1506 - (29%)
• Similarity: 612/1506 - (40%)
• Gaps: 443/1506 - (29%)

  Fly     1 METVVAFNNELSGLYDSRPPISKAKMAAITKSAMRAIKLYKHVVQSVEKFILKCKPEYKVPGLYV 65
            ||.|.|||.||..|.:.:||||||||..|||||::|||.||||||||||||.|||||||||||||
Zfish     1 MEAVKAFNGELYSLNEYKPPISKAKMTQITKSAIKAIKFYKHVVQSVEKFIQKCKPEYKVPGLYV 65

  Fly    66 IDSIVRQSRHQYGMDKDLFAPRFQRNLTETFANLFRCAPEDKSRIIRVLNLWQKNNVFKSEVIQP 130
            |||||||||||:|.:||:|||||.:|:..||.||:||..:|||:|:||||||||||||||::|||
Zfish    66 IDSIVRQSRHQFGQEKDVFAPRFSKNIIGTFQNLYRCPTDDKSKIVRVLNLWQKNNVFKSDIIQP 130

  Fly   131 IFDLADPNHPIYHQMPPVGGSGG-QGGGVGPGPSSSGA----------------------LSLAD 172
            :.|:|....|  ..:.||..|.. ......||..::.|                      .::|.
Zfish   131 LLDMAAGLPP--PSVTPVSASSVIPMNSTTPGTPATPATPANIVQSLPDWASQISNTDTVAAVAQ 193

  Fly   173 ISSGPNG--LNSSGMEISMNSSGGDDKM-----GGAMPDLSLGHEKFGGGSSSSKRHYSEQHYSK 230
            |...|.|  |......:.|........:     .|.:..|.....:....::::|.:..||..|
Zfish   194 ILQSPQGQQLQQLVQSLQMQQQKPQPSLLQALDAGLVVQLQALTAQLTAAATANKLNPLEQRVS- 257

  Fly   231 RQSVVSCSSKTSKSHHHKREYVKSSHDIDYQVREIIEEEDEGLQMLMADDHHCMGDDSPPTSSNL 295
                 |.:.|.........|   |.|..|       .::|.....:             |..|:.
Zfish   258 -----SFNKKLLGQFDFGNE---SEHSED-------SKKDSASSQI-------------PVLSDS 294

  Fly   296 LDEKKIKQLLNNPNVLRQLHTLQNFQNLKPQEENQKHRYQDEALQQHFQNVMKGNA-GMPPGMGM 359
            ::.....||..:    .|...|:.||  |...|:|:|  |.:::....|:.:.|.. ..|.|...
Zfish   295 INSSIFHQLAEH----LQQQNLEQFQ--KQLMEHQQH--QQKSMSMESQDAIFGTENSAPSGQSS 351

  Fly   360 G--------MNMNDSMDLNKDVEFISEQQTIEVINLDGGDSRSPTPDRDRYK---------RSRR 407
            .        ..|:||||        ::||.:::      |......|.:.::         |||.
Zfish   352 APSQHQVQESKMDDSMD--------NQQQDMDI------DEGQDVVDEELFETDEKKTSSTRSRT 402

  Fly   408 NSRSRTRSPRGRGAGGGTGNDRRRRGTRSRSRSRSPRSSRRRGSRDRDRMDRSNR---DKERDRE 469
            .||||:|||             :||.:||||.||..:..:|..||.|||..:|:|   .:.|.||
Zfish   403 RSRSRSRSP-------------KRRRSRSRSGSRKRKHRKRSRSRSRDRKRKSSRSYSSERRARE 454

  Fly   470 HERERRKKGLPDIKKEHLSVCSTTLWVGHLSKLVYQEELSDTFGEYGDIVSIDQIVPRGCAFIVM 534
            .|::|:|||||.|:...|||||||||||.:.|...|::|::.|.|:|.|.||:.|.|||||:|.|
Zfish   455 REKDRQKKGLPPIRSRVLSVCSTTLWVGQVDKKATQQDLTNLFEEFGQIESINMIPPRGCAYICM 519

  Fly   535 NRRQDAHKAMQALK--NHKLQGRAITISWAAGKGVKSKEWKDYWDLELGVTYIPWSKLSSDTDFD 597
            ..||||::|:|.|.  ::|:..:.|.|:||..|||| :|:|.:||::||||||||.|:..| |.|
Zfish   520 VHRQDAYRALQKLSTGSYKIGSKVIKIAWALNKGVK-QEYKQFWDVDLGVTYIPWEKVKLD-DLD 582

  Fly   598 ALEEGGMFDEDTMPIQMKQKINQAKNAAKEHKGASI-AEAAGAPSVG--------------VPPP 647
            ...||||.|::|:....:.:.|  ...|||.....: |||:.|.|..              :|.|
Zfish   583 DFAEGGMIDQETVNNDWESQRN--TETAKETPSQPVKAEASTASSTASEAFTQPVTMMPMQIPVP 645

  Fly   648 NLIFGIDTTQPPPVGPVGPVGPPPGPGAPPPGLMGMVRGQFPMAPPMGINMPPPIMMPPTNMPPP 712
            ..:..:...  ||..||....||||.|.|||.|..            |.|         .:.|||
Zfish   646 QTVPAVGLV--PPSFPVTMAMPPPGFGPPPPFLRA------------GFN---------ASQPPP 687

  Fly   713 MMMPTTNMPPPMMMPPTMMPPGFPGIGGPPHPMA------LPPGAPFPPPGAVPPP-IPS---GG 767
            ..|.|..:.|..:...|.:......:..|..|..      .|.||..||.|.:|.. |||   |.
Zfish   688 GFMATPGVGPSAVGAVTSLASAQNSLVQPAMPNTQDSSKDSPFGAMMPPTGTIPGSFIPSAMPGA 752

  Fly   768 QISNSGNVSDDQMDIEMDLEDAPPLPPQ-QQANFNPSPSNVDLSPAAMANEMFQQRDRERDRDNR 831
            |:.|                   |:..| ||::.:.:|.:.|...|  ::|:..|
Zfish   753 QVFN-------------------PVGAQHQQSSTDKTPQSADAMEA--SSEITLQ---------- 786

  Fly   832 DRSRGPGNSRWGGRDDVVEAAERWRAENGGGGGGPG-SGLGPNAAFNEARA---RLNLNPIEHGM 892
                        |..:.|.:             |.| .|:.|:|:.....|   ...|.|:|
Zfish   787 ------------GMQNAVRS-------------GMGLLGMHPSASLTHPLAGQRMTGLLPLE--- 823

  Fly   893 PRPDFMDFDNRGGPGGPRGMGPRGNHNGGGPGGDF---FPPNMNHN-------------RFNQPT 941
            .||:.:.                      ||||.|   ..|.:.|.             |...|.
Zfish   824 LRPNLLQ----------------------GPGGRFPLLMQPGLAHQTNNVLDASLQAQARLRAPF 866

  Fly   942 SLMQ---------MRIP-PPASFN------------------QRMGG------PAGNGGNGV--- 969
            |.|:         ||.| ||.:.|                  |:.|.      |......|:
Zfish   867 SQMEQFNRTEGTFMRAPNPPPAVNDSLIKPNNEAPPRSDVVTQQEGDQDYRFPPPEKQSTGLLKT 931

  Fly   970 GPMFMRNQGGGPGGAGPS--GGGPGGAGTGGGGPGGRQQ-----------GPGFFNPRNPFNDNQ 1021
            .|:.||.:   ||.|.|.  ...|..........|.|:.           ||    ||..|  ::
Zfish   932 PPLEMRTE---PGPARPPLLERPPRAVMQAESREGRRENVSAGFRAESRWGP----PRGDF--DE 987

  Fly  1022 RGRGGQSGGGVRGVGGGGGGGPGGRGRWSDDEDEGGNNFKRR-----GGPGGPGGNRFRGERGGE 1081
            |...|.|||..:|.                .|:...|||:.|     ||..||..||..|.....
Zfish   988 RDMRGMSGGSAKGF----------------QEERPNNNFQNRFEGRSGGSSGPTWNRGTGPSFNA 1036

  Fly  1082 MM----DDRRGNNSRGGRGGPREDRERPGFGNRRGSRDDSNRHSISSTDEGNKAASETEPKPKNV 1142
            .|    ||||   ....|...|:||:....|...|     ||||      ..:.......:.:|.
Zfish  1037 NMNQDYDDRR---RPWERQKDRDDRDMDFRGEMNG-----NRHS------RERDRDRERERDRNR 1087

  Fly  1143 ETELGSTNTGAPAAATRVDTEEDWDQELQDYEARMEAQKPGER----ASQS----ANSPPQEKLP 1199
            |.|.|                .|.::| :|.|...|..|..||    :|||    ..:.||..||
Zfish  1088 ERERG----------------RDRNRE-RDRERENEKDKERERWTPLSSQSQPLLPQAKPQPLLP 1135

  Fly  1200 AENENFAKPADVTGDPLVAKSQTETSAACTPLYDELPPPAVPQTPTQPPKEPQGS-PPRP---AP 1260
            .......:|.        |:...:|::..:|:   |.|...||: |:|..|||.. .|.|   :|
Zfish  1136 LPTPLLPRPP--------AQEPVQTNSQSSPV---LHPDPQPQS-TEPAAEPQAEVKPEPQSLSP 1188

  Fly  1261 PV----------------IETEAAPHEE----------SVPAP-----PAELQTEAPSAEEPIAP 1294
            |.                :||::.|..|          |.|.|     ||..:.|.|:.|.|...
Zfish  1189 PEETTSSQEDTAASCVNGMETDSKPQSEPASQTATEPSSSPYPSLTSTPACFENEPPNGENPPEM 1253

  Fly  1295 AASADVVAEAD 1305
            :.||.|.|:|:
Zfish  1254 SVSASVDADAE 1264

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Isha	NP_001097394.1	PHA03247	<1187..1306	CDD:223021	42/158 (27%)
		CID_SCAF8_like	6..135	CDD:340780	95/128 (74%)
		RRM_SCAF4_SCAF8	490..566	CDD:409674	37/77 (48%)
scaf8	NP_001423676.1	CID_SCAF8	5..135	CDD:340801	95/129 (74%)
		RRM_SCAF8	475..553	CDD:409895	37/77 (48%)
		PRK12323	<1146..1286	CDD:481241	34/123 (28%)