Protein Alignment Isha and Scaf4

DIOPT Version :10

• Sequence 1: NP_001097394.1
• Gene: Isha / 37411
• FlyBaseID: FBgn0034598
• Length: 1306
• Species: Drosophila melanogaster
• Sequence 2: NP_001032424.2
• Gene: Scaf4 / 245924
• RGDID: 727896
• Length: 1200
• Species: Rattus norvegicus
• Alignment Length: 1414
• Identity: 422/1414 - (29%)
• Similarity: 548/1414 - (38%)
• Gaps: 441/1414 - (31%)

  Fly     1 METVVAFNNELSGLYDSRPPISKAKMAAITKSAMRAIKLYKHVVQSVEKFILKCKPEYKVPGLYV 65
            |:.|.|||.||..|.|.:||||:|||..|||:|::||||||||||.|||||.|||||||||||||
  Rat     1 MDAVNAFNQELFSLMDMKPPISRAKMILITKAAIKAIKLYKHVVQIVEKFIKKCKPEYKVPGLYV 65

  Fly    66 IDSIVRQSRHQYGMDKDLFAPRFQRNLTETFANLFRCAPEDKSRIIRVLNLWQKNNVFKSEVIQP 130
            |||||||||||:|.|||:|.|||..|:|.||..|:.|..||||:|:|||||||||.|||.|:|||
  Rat    66 IDSIVRQSRHQFGTDKDVFGPRFSENITATFQYLYLCPSEDKSKIVRVLNLWQKNGVFKIEIIQP 130

  Fly   131 IFDLADPNHPIYHQMPPVGGSGGQGG--------GVGPGP-SSSGALSLADISSGPNGLNSSGME 186
            :.|:|            .|.|....|        |..|.| ..|..|..|..:|.|
  Rat   131 LLDMA------------AGTSNAAPGAENVTNNEGSPPPPVKVSSELPQAPTNSMP--------- 174

  Fly   187 ISMNSSGGDDKMGGAMPDLSLGHEKFGG---------GSSSSKRHYSEQHYSKRQS--------- 233
                          |:|.|. ..:.|..         |....:...:.|...|.||
  Rat   175 --------------AVPQLP-SSDAFAAVDQLFQTTQGQQLQQILQTFQQPPKPQSPALDSAVMA 224

  Fly   234 ---VVSCSSKTSKSH--HHKREYVKSSHD-IDYQVREIIEEEDEGLQMLMADD-----HHCMGDD 287
               .::...||:.:.  ..|..:.|...| .||      ::|.|.::....:|     ...:...
  Rat   225 QVQAITAQLKTAPTQPPEQKTAFDKKLLDRFDY------DDEPEAVEDSKKEDAVAVATTALATA 283

  Fly   288 SPPT-------------SSNLLDEKKIKQLLNNP--NVLRQLHTL-QNFQNLKP-----QEENQK 331
            :||.             :|:.......:.....|  .:.:..:|. ||....:|     |:::.:
  Rat   284 APPVPAAATPAVAPAVPASSATSPPPPQAPFGYPGDGMQQPAYTQHQNVDQFQPRMMALQQDSMQ 348

  Fly   332 HRY-------------------------------------------------------------- 334
            |:.
  Rat   349 HQVPLPPNGQMPGFGLLSAPPPFPPMPQPGMPQPGMPQPGMPQPGLSQPGLPQPGMPQPGMPQPG 413

  Fly   335 -------QDEALQQHFQNVMKGNAGMP-PGM---GM---GMNMNDSMDLNKDVEFISE------- 378
                   |....|...........||| |||   ||   ||.....:.......|..:
  Rat   414 MPQPGMPQPGLAQPGLAQPGMPQPGMPQPGMPQPGMPQPGMPPTPPVQPTFQPTFQPQNEPHSQK 478

  Fly   379 --QQTIEVINLDGGDSRSPTPDRDRYKRSRRNSR-SRTRSPRGRGAGGGTGNDRRRRGTRSRSRS 440
              ||.:||          ..|.....||....|| ||:|||:.|               ||||.|
  Rat   479 PHQQEMEV----------EQPCVTEVKRHVPESRKSRSRSPKRR---------------RSRSGS 518

  Fly   441 RSPRSSRRRG-SRDRDR---MDRSNRDKERDREHERERRKKGLPDIKKEHLSVCSTTLWVGHLSK 501
            ||.||..||. ||.|||   ..||...:.||||.|||||:||||.||.|..||||||||||.|.|
  Rat   519 RSRRSRHRRSRSRSRDRRRHSPRSRSQERRDREKERERRQKGLPQIKSETASVCSTTLWVGQLDK 583

  Fly   502 LVYQEELSDTFGEYGDIVSIDQIVPRGCAFIVMNRRQDAHKAMQALK--NHKLQGRAITISWAAG 564
            ...|::::....|:|.|.||:.|.|||||:|||..||||::|:|.|.  |:|:..::|.|:||..
  Rat   584 RTTQQDVASLLEEFGPIESINMIPPRGCAYIVMVHRQDAYRALQKLSRGNYKVNQKSIKIAWALN 648

  Fly   565 KGVKSKEWKDYWDLELGVTYIPWSKLSSDTDFDALEEGGMFDEDTMPIQMKQKINQAKNAAKEHK 629
            ||:|: ::|.|||:|||||||||.|:.:: :.::..||||.|.||:....|....:.:|...::.
  Rat   649 KGIKA-DFKQYWDVELGVTYIPWDKVKAE-ELESFCEGGMLDSDTLNPDWKGIPKKPENEVAQNG 711

  Fly   630 GASIAEAAGAPSVGVPPPNLIFGIDTTQPPPVGPV-GPVGPPPGPGAPP--PGLMGMVRGQFPMA 691
            ||..:.......:..|.|         .|.|..|| .|:..|| |..||  ||
  Rat   712 GAEASHTEPVSPIPKPVP---------VPVPALPVPAPITVPP-PQVPPHQPG------------ 754

  Fly   692 PPMGINMPPPIMMPPTNMPPPMMMPTTNMPPPMMMPPTMMPPGFPGIGGPPH--PMALPPGAPFP 754
            ||:...:.||...||..:|||...|  .:|||...||....|||    .|.|  |..||||.|.|
  Rat   755 PPVVGALQPPAFTPPLGIPPPGFGP--GVPPPPPPPPPFWGPGF----NPMHLPPGFLPPGPPPP 813

  Fly   755 --PPGAVPPP------IPS--GGQISNSGNVSDDQMDIEMDLEDAP---PLPPQQQANFNPSPSN 806
              ||.::|||      ||:  .|...|:.:....:|........||   |.||..|
  Rat   814 ITPPVSIPPPHTPPISIPNLVSGARGNAESADSAKMYGSAGPPAAPTSLPTPPVTQ--------- 869

  Fly   807 VDLSPAAMANEMFQQRDRERDRDNRDRSRGPGNSRWGGRDDVVEAAERWRAENGGGGGGPGSGLG 871
                |.::.                                            |..|..||..:|
  Rat   870 ----PVSLL--------------------------------------------GTQGVAPGPVIG 886

  Fly   872 PNA-AFNEARARLNLNPIEH--GMPRPDFMDFDNRGGPGGPRGMGPRGNHNG--GGPGGDFFPPN 931
            ..| :......|..|.|::.  |||.|....|...    .||.|.|...|.|  .||||
  Rat   887 LQAPSTGLLGGRPGLIPLQRPPGMPPPHLQRFPMM----PPRPMPPHMMHRGPPPGPGG------ 941

  Fly   932 MNHNRFNQPTSLMQMRIPPPASFNQRMGGPAGNGGNGVGPMFMRNQGGGPGGAGPSGGGPGGAGT 996
                          ..:|||    ..|.||....|..|.|      ||.||..|| |.||||:..
  Rat   942 --------------FAMPPP----HGMKGPFPPHGPFVRP------GGMPGLGGP-GPGPGGSED 981

  Fly   997 GGG-----------------------------------GPGGRQQ-GPGFFNPRNPFNDNQR--- 1022
            ..|                                   .|...|| .|....|:...|||::
  Rat   982 RDGRQQQPQQQQQQQQQQQQQQQQQQQQPPPQQSQTQQQPAPSQQPAPAQQQPQQFRNDNRQQFN 1046

  Fly  1023 -GRGGQSGGGVRGVGGGGGGGPGGRGRWSDDEDEGGNNFKRRGGPGGPGGNRFRGERGGEMMDDR 1086
             || .|...|.|..|..........|..||:.| ..|..:|..|...|..:|.|..       :.
  Rat  1047 SGR-DQERFGRRSFGSRVENDRERYGSRSDERD-NSNRERREWGRRSPERDRHRDL-------EE 1102

  Fly  1087 RGNNSRGGRGGPREDRERPGFGNRRGSRDDSNRHSISSTDEGNKAASETEPKPKNVE---TELGS 1148
            |...|.|.|...|:.|:|.....:..|| ...:|.::....||||          ||   :::|:
  Rat  1103 RSRRSSGHRDRDRDSRDRESRREKEESR-GKEKHEVADRAGGNKA----------VEAPLSQVGN 1156

  Fly  1149 TNTGAPAAATRVDTEEDWDQELQDYEARMEAQKPGERASQSANSPPQEK 1197
            |:|.:               ||...||.....||.|..:::.:|...||
  Rat  1157 TDTVS---------------ELNKGEAMATVVKPEESPAEATSSVEPEK 1190

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Isha	NP_001097394.1	PHA03247	<1187..1306	CDD:223021	3/11 (27%)
		CID_SCAF8_like	6..135	CDD:340780	93/128 (73%)
		RRM_SCAF4_SCAF8	490..566	CDD:409674	38/77 (49%)
Scaf4	NP_001032424.2	CID_SFRS15_SCAF4	5..135	CDD:340802	93/129 (72%)
		PRK12323	<124..>266	CDD:481241	36/183 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	140..172		7/31 (23%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	299..324		2/24 (8%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	346..566		61/244 (25%)
		RRM_SCAF4	570..650	CDD:409894	40/79 (51%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	696..724		4/27 (15%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	834..875		10/97 (10%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	927..1200		81/330 (25%)
		SF-CC1	1049..>1140	CDD:273721	25/100 (25%)