Protein Alignment Topors and TOPORS

DIOPT Version :8

• Sequence 1: NP_001261083.1
• Gene: Topors / 37188
• FlyBaseID: FBgn0267351
• Length: 1038
• Species: Drosophila melanogaster
• Sequence 2: NP_005793.2
• Gene: TOPORS / 10210
• HGNCID: 21653
• Length: 1045
• Species: Homo sapiens
• Alignment Length: 1125
• Identity: 248/1126 (22%)
• Similarity: 394/1126 (35%)
• Gaps: 330/1126 (29%)

  Fly    15 YLGVDELGASVIVEPGLEGSNAGGRTLPAAAIKFADLTESGSESGDNEAEE--PVSAGPDNANAI 77
            :||..||.||....|....|.     :.|:|.|...:.....::|.::.::  |..|.||:
Human    46 FLGCRELAASAPARPAPASSE-----IMASAAKEFKMDNFSPKAGTSKLQQTVPADASPDS---- 101

  Fly    78 GEPGTSASAAEENGTVERNSPPPNCAICLSRCRRKCFTDSCMHQFCFKCLCEWSKIKPECPLCKQ 142
                                   .|.|||.|.....:.|.|:|:|||:|:.||||.|.|||||||
Human   102 -----------------------KCPICLDRFDNVSYLDRCLHKFCFRCVQEWSKNKAECPLCKQ 143

  Fly   143 PFRTIIHNVRTLDDYDRYPVQTTSPVPTENPS-------LRYHI----------------VRRPR 184
            ||.:|.|:||..||:..|.::     |:.|.|       .||..                |.|..
Human   144 PFDSIFHSVRAEDDFKEYVLR-----PSYNGSFVTPDRRFRYRTTLTRERNASVYSPSGPVNRRT 203

  Fly   185 YTP-----LVQNQAVIVNDIEAAIAAGAAGEDVLSAAEVAAGRRSYSRFEPYRSELMNYYQHDQD 244
            .||     |.:...:.....:..|....  ..:.......|..||..:.:  ..:::|:
Human   204 TTPPDSGVLFEGLGISTRPRDVEIPQFM--RQIAVRRPTTADERSLRKIQ--EQDIINF------ 258

  Fly   245 ASTSGSLSQLWRRYVYDRKLYALPVSDSVTGHFREWSARFYRNNPAQIHRLMPWIHRDIMCLLRN 309
                       ||.:|........:.|.  |.:|:.||.|:|.|||.:|||:||:.|::..|...
Human   259 -----------RRTLYRAGARVRNIEDG--GRYRDISAEFFRRNPACLHRLVPWLKRELTVLFGA 310

  Fly   310 AAHSVNTVMTLMSDLLPMTSLLGPTFRRRLSPYLGERTSHFIHELFNFARSPYDINGYDHVVQYS 374
            ....||.|..::...:....|....|...|.|:|..||.|||||..:|||||:::..:|....|
Human   311 HGSLVNIVQHIIMSNVTRYDLESQAFVSDLRPFLLNRTEHFIHEFISFARSPFNMAAFDQHANY- 374

  Fly   375 ARVAEEVEVDLLDMVETQSSNGDDLNLEVGDSDADAINAGFSPDWSPPRVRPSTSVIVTNPGATH 439
                                          |..|.:...|...|         :|||..:|....
Human   375 ------------------------------DCPAPSYEEGSHSD---------SSVITISPDEAE 400

  Fly   440 S-------FSVTMASDGSELPGISIRRTTNVGSQTVAINLSMRRPAAVASEEPEVIEIDDGDAAA 497
            :       .:|:.|....|.||.|..     .|:.|.:.:|.....:.:|:|    |:..|.|.:
Human   401 TQELDINVATVSQAPWDDETPGPSYS-----SSEQVHVTMSSLLNTSDSSDE----ELVTGGATS 456

  Fly   498 NAEVAAINDGSNTSRRHAGATLPVTAHIELESSSSSGDEDECVFVLELKPPHMRTPEQVSLDSNS 562
            ..:....||..|.                 :|..||   |.||.|..:||...||||.|.|.|:|
Human   457 QIQGVQTNDDLNN-----------------DSDDSS---DNCVIVGFVKPLAERTPELVELSSDS 501

  Fly   563 DS-------DVVFVNEQHEAAPDAIAENRSTQSPLDLASRDQGLFMGPSTSGAAANRGKNWKLVM 620
            :.       :.|...||.::.....::.....||..:..:|:.:..|...|
Human   502 EDLGSYEKMETVKTQEQEQSYSSGDSDVSRCSSPHSVLGKDEQINKGHCDS-------------- 552

  Fly   621 AQTRRLDQLRTLRSIRSKKSRRSSMPARSDSGSSPSSCSSS-----------SFHFSSSSDEDSS 674
             .||          |:|||..:     ||.|.|||.:.:||           ..|........|.
Human   553 -STR----------IKSKKEEK-----RSTSLSSPRNLNSSVRGDRVYSPYNHRHRKRGRSRSSD 601

  Fly   675 DSSTTNSEPPKKKSRKRVANNKRSKKESIGKRTSRKRKAKDQNMEMLEQQQISQKKP--QRQPES 737
            ..|.:.|...:|..||.....:...|.|..:.:||.|..:|:..........|::..  ....||
Human   602 SRSQSRSGHDQKNHRKHHGKKRMKSKRSRSRESSRPRGRRDKKRSRTRDSSWSRRSQTLSLSSES 666

  Fly   738 SSDSPSSSDDESGGDSSESSGQPN---TNNNKS---------SSDSDDDSAVNMQLSALRATLKA 790
            :|.|.|.|.|. |...|.|..:..   .||..|         |.:.|.|   ..:.|..|.||..
Human   667 TSRSRSRSSDH-GKRRSRSRNRDRYYLRNNYGSRYKWEYTYYSRNKDRD---GYESSYRRRTLSR 727

  Fly   791 EATLEDRKPVKLELQLPDDDQAGPLHSRMTPSPREDNEPGCSAPKRRRSCSHSN------QSSQS 849
            ..                       :||.:.||....:........|:..:||.      :..:|
Human   728 AH-----------------------YSRQSSSPEFRVQSFSERTNARKKNNHSERKYYYYERHRS 769

  Fly   850 ASLASSSTATSSSAPLSSFAWGAAGFSGDPLMRGHPAMEE----------HDIANSLIELSTLTQ 904
            .||:|:.:.|:|:..             |.:....|..:.          :::|....|.::..:
Human   770 RSLSSNRSRTASTGT-------------DRVRNEKPGGKRKYKTRHLEGTNEVAQPSREFASKAK 821

  Fly   905 PVNIGLFNEHYNSAENSMGMLSNTLCDSPQTLAADDANLENYFDTDADPEAS--------RERDA 961
                   :.||..:.:.:                 |.|.:|..||.:|..:|        |:|..
Human   822 -------DSHYQKSSSKL-----------------DGNYKNESDTFSDSRSSDRETKHKRRKRKT 862

  Fly   962 YSLEAAIDVVGESELQIAEDTATATEEQDEEDEEDEDQEEDDQEEEK-------AAEEEEEEEED 1019
            .||  ::::|.|.:   |.||....:::.::.::...:...|.....       :..:::.|.::
Human   863 RSL--SVEIVYEGK---ATDTTKHHKKKKKKHKKKHKKHHGDNASRSPVVITIDSDSDKDSEVKE 922

  Fly  1020 DDDSDNHDEND--ENQGLLP 1037
            |.:.||....|  :|:.|.|
Human   923 DTECDNSGPQDPLQNEFLAP 942

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Topors	NP_001261083.1	RING	102..144	CDD:238093	25/42 (60%)
TOPORS	NP_005793.2	E3 ubiquitin-protein ligase activity	1..195		54/186 (29%)
		Required for DNA-binding	51..374		103/383 (27%)
		RING	102..145	CDD:238093	25/43 (58%)
		Interaction with SUMO1. {ECO:0000269|PubMed:14516784}	437..654		60/271 (22%)
		Required for sumoylation and localization to discrete nuclear foci	437..574		45/191 (24%)
		Interaction with TOP1. {ECO:0000269|PubMed:10352183}	456..882		111/545 (20%)
		Interaction with p53/TP53. {ECO:0000269|PubMed:10415337}	456..731		76/352 (22%)
		Interaction with UBE2I. {ECO:0000269|PubMed:14516784}	854..917		10/68 (15%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930				C314179848
eggNOG	1	0.900				E1_KOG4430
Hieranoid	1	1.000
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	169	1.000	Inparanoid score	I4084
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000				FOG0005422
OrthoInspector	1	1.000				T1073663
orthoMCL	1	0.900				OOG5_133270
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910		0.958	Consistency score
RoundUp	1	1.030		avgDist	Average_Evolutionary_Distance	R9253
TreeFam	1	0.960
User_Submission	0	0.000	Not matched by this tool.
	10	9.680