Protein Alignment POSH and sh3rf1

DIOPT Version :10

• Sequence 1: NP_523776.1
• Gene: POSH / 36990
• FlyBaseID: FBgn0040294
• Length: 838
• Species: Drosophila melanogaster
• Sequence 2: NP_001038952.2
• Gene: sh3rf1 / 556523
• ZFINID: ZDB-GENE-030131-6288
• Length: 880
• Species: Danio rerio
• Alignment Length: 949
• Identity: 291/949 - (30%)
• Similarity: 430/949 - (45%)
• Gaps: 198/949 - (20%)

  Fly     1 MDEHTLNDLLECSVCLERLDTTSKVLPCQHTFCRKCLQDIVASQHKLRCPECRILVSCKIDELPP 65
            |||..|.|||||.|||||||.|:|||||||||||:||..||.|:.:|||||||.||...:||||.
Zfish    14 MDESALLDLLECPVCLERLDATAKVLPCQHTFCRRCLLGIVGSRGELRCPECRTLVESGVDELPS 78

  Fly    66 NVLLMRILEGMKQ---------------NAAAGKGEEKGEETETQPERAKPQPPAESVAPPDNQL 115
            |:||:|:|:|:||               :|.||...:....::..|            .|...|.
Zfish    79 NILLVRLLDGIKQRPRRTGSVHGTCANGSAVAGVRAQGAGGSQRDP------------GPTGGQS 131

  Fly   116 LQLQSHQQSHQPARHKQRRFLLPHAYALFDFASGEATDLKFKKGDLILIKHRIDNNWFVGQANGQ 180
            .::|:   ...|.|...:   ||.|.||:::...|..||||.|||:|:::.::|.||:.|:..|.
Zfish   132 QRVQA---KSTPVRGVPQ---LPCAKALYNYDGKEPGDLKFSKGDIIILRRQVDENWYHGEMGGV 190

  Fly   181 EGTFPINYVKVSVPLPM--PQCIAMYDFKMGPND-EEGCLEFKKSTVIQVMRRVDHNWAEGRIGQ 242
            .|.||.|:|:|..|||.  |||.|:|||::...: ::.||.|.|..::.|:||||.|||||.:|.
Zfish   191 HGFFPTNFVQVIKPLPQPPPQCKALYDFELKDKEADKDCLPFSKDDILTVIRRVDENWAEGMLGD 255

  Fly   243 TIGIFPIAFVELNAAAKKLLDSGLHTHPFCHPPKQQGQRALPPVPVIDPTVVTESSSG----SSN 303
            .||||||::||.|:||::|::       ...|.:..|..:..|         :.||||    .|.
Zfish   256 KIGIFPISYVEFNSAARQLIE-------LDKPSEGGGDSSEGP---------SSSSSGPQANGSQ 304

  Fly   304 STPG-SSNSSSTSSSNNCSPNHQISLPNTPQH--VVASGSASVRFRDKGAKEKRHSLNALLGGGA 365
            ..|| ..||....|..:.:.:|:..|...||.  :..||...:...:..|..:...|:..|...|
Zfish   305 KAPGEKKNSKKRHSFTSLTMSHKPCLAPPPQRHSMEISGPVLISSSNPTAAARIGELSGGLSSSA 369

  Fly   366 P-------LSLLQTNRHS-----AEILSLPHELSRLEVSSSTALKPTSAP----QTSRVLKTTVQ 414
            |       ..|:.|...|     |.:.:.|.|.|...:... ||.|...|    |:|.|....:.
Zfish   370 PSQVHICTTGLIVTPPPSSPVTTATVFTFPPETSYASIPVD-ALPPPPPPPPQSQSSVVGAAALN 433

  Fly   415 QQMQPN----------LPWGYLALFPYKPRQTDELELKKGCVYIVTERCVDGWFKGKNW-LDITG 468
            ...:|:          .|..|:|:|||.||:.|||||:||.:::|.|||.||||||.:. ....|
Zfish   434 AGQRPSPAAGDQSGRQRPTVYVAMFPYSPRKEDELELRKGEMFLVLERCQDGWFKGTSMHTGKIG 498

  Fly   469 VFPGNYLTP----LRARDQQQLMHQWKYVPQNADAQMAQVQQHPVAPDVRLNNMLSMQPPDLPPR 529
            ||||||::|    :....|.:       ||....:|..: ....|:|...|.:|...:|..:.|.
Zfish   499 VFPGNYMSPVSRTVSGSSQPK-------VPLTLCSQAGR-GVTIVSPSSALGSMDLSKPLPVCPN 555

  Fly   530 QQQATATTTSCSVWSKPVEAL-FSRKSEPKPETATASTTSSSSSGAVGLMRRLTHMKTRSKSPGA 593
                  .|.|||:.:..|.|. ......||                  ::..:|...|.:::
Zfish   556 ------ATPSCSLPAAVVTAAHLPTGQHPK------------------VLMHVTSQMTVNQA--- 593

  Fly   594 SLQQVPKEAISTNVEFTTN-PSAKLHPV--HVRSGSCPSQ---LQHSQPLNETPAAKTA------ 646
                  :.|:.|.|..:.: |:|.:.|:  |......||.   ||.|..||.:....:|
Zfish   594 ------RNAVRTAVSHSQDRPTAAVTPIQSHNPVAYLPSTAVVLQASPVLNSSSGCSSARVGVAL 652

  Fly   647 -AQQQQFLPKQLPSASTNS------------VSYGSQRVKGSKERPHLICARQSLDA-----ATF 693
             .......|..:.:||.::            |:.||.:..|:... |.:..|...|.     ...
Zfish   653 GCAAASLTPPNVSAASLDTDAMRPVPMVALPVNAGSTKPLGAASN-HGVACRLDKDCKREKKGLL 716

  Fly   694 RSMYNNAASPPPPTTSVAPAVYAGGQQQV---IPGGGAQSQL-------HANMI--------IAP 740
            :.:.|.....|.|.:|  |.:.|  :|.|   :|.|....::       |...|        ::.
Zfish   717 KLLSNKKKLRPSPPSS--PTLEA--EQSVSMELPQGAVGPEMALSGSAGHNGRIGACPMDSELSM 777

  Fly   741 SHRKSHSLDASHVLSPSSNMI---------TEAAIKASATTKSPYCTRESRFRCIVPYPPNSDIE 796
            |...|::...:|..||..|..         ..:.:......:...|   .|:|.:|.|||.|:.|
Zfish   778 SSSSSNTDAVTHRSSPQDNTAPIAPPPRQPCSSLLSMQHDGRPIVC---ERYRVVVSYPPQSEAE 839

  Fly   797 LELHLGDIIYVQRKQKNGWYKGTHARTHKTGLFPASFVE 835
            |||..|||::|.:|:::||:|||..|..:|||||.|||:
Zfish   840 LELKEGDIVFVHKKREDGWFKGTLQRNGRTGLFPGSFVD 878

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
POSH	NP_523776.1	RAD18	6..>113	CDD:227719	56/121 (46%)
		RING-HC_SH3RF3	8..53	CDD:438408	34/44 (77%)
		SH3	139..191	CDD:473055	22/51 (43%)
		SH3_SH3RF_2	199..251	CDD:212721	27/52 (52%)
		SH3_SH3RF_3	424..477	CDD:212717	31/53 (58%)
		SH3_SH3RF_C	782..836	CDD:212719	30/54 (56%)
sh3rf1	NP_001038952.2	RING-HC_SH3RF1	21..68	CDD:438406	36/46 (78%)
		SH3_SH3RF1_1	148..201	CDD:212860	23/52 (44%)
		SH3_SH3RF1_2	211..265	CDD:212863	27/53 (51%)
		SH3_SH3RF1_3	453..507	CDD:212859	31/53 (58%)
		SH3_SH3RF_C	825..879	CDD:212719	30/54 (56%)