Protein Alignment POSH and Sh3rf1

DIOPT Version :10

• Sequence 1: NP_523776.1
• Gene: POSH / 36990
• FlyBaseID: FBgn0040294
• Length: 838
• Species: Drosophila melanogaster
• Sequence 2: NP_942059.1
• Gene: Sh3rf1 / 306417
• RGDID: 735154
• Length: 894
• Species: Rattus norvegicus
• Alignment Length: 1016
• Identity: 301/1016 - (29%)
• Similarity: 436/1016 - (42%)
• Gaps: 305/1016 - (30%)

  Fly     1 MDEHTLNDLLECSVCLERLDTTSKVLPCQHTFCRKCLQDIVASQHKLRCPECRILVSCKIDELPP 65
            |||..|.|||||.|||||||.::||||||||||::||..||.|:::|||||||.||...:||||.
  Rat     1 MDESALLDLLECPVCLERLDASAKVLPCQHTFCKRCLLGIVGSRNELRCPECRTLVGSGVDELPS 65

  Fly    66 NVLLMRILEGMKQ---NAAAGKGEEKGEETETQPERAK------------------PQPPAESVA 109
            |:||:|:|:|:||   ....|.|   |..|.|...||:                  .||..::.:
  Rat    66 NILLVRLLDGIKQRPWKPGPGGG---GSTTCTNVLRAQGSTVVNCGSKDLQSPQCGQQPRVQAWS 127

  Fly   110 PPDNQLLQLQSHQQSHQPARHKQRRFLLPHAYALFDFASGEATDLKFKKGDLILIKHRIDNNWFV 174
            ||...:.|                   ||.|.||:::...|..||||.|||:|:::.::|.||:.
  Rat   128 PPVRGIPQ-------------------LPCAKALYNYEGKEPGDLKFSKGDIIILRRQVDENWYH 173

  Fly   175 GQANGQEGTFPINYVKVSVPLPM--PQCIAMYDFKMGPND-EEGCLEFKKSTVIQVMRRVDHNWA 236
            |:.||..|.||.|:|::..|||.  |||.|:|||::...: ::.||.|.|..|:.|:||||.|||
  Rat   174 GEVNGVHGFFPTNFVQIIKPLPQPPPQCKALYDFEVKDKEADKDCLPFAKDDVLTVIRRVDENWA 238

  Fly   237 EGRIGQTIGIFPIAFVELNAAAKKLLDSGLHTHPFCHPPKQQGQRALPPVPVIDPTVVTESSSGS 301
            ||.:...||||||::||.|:|||:|::..                 .||||.:|     .:...|
  Rat   239 EGMLADKIGIFPISYVEFNSAAKQLIEWD-----------------KPPVPGVD-----TAECPS 281

  Fly   302 SNSTPGSSNSSSTSSSNNCSPNHQISLPNTPQHVVASGSASVRFRDKGAKEKRHSLNALLGGGAP 366
            :.:...||.|..:.:..|....|..:            |.::..:...|.:.|||:..    ..|
  Rat   282 ATAAQSSSASKHSDTKKNTRKRHSFT------------SLTMANKSSQASQNRHSMEI----SPP 330

  Fly   367 LSLLQTNRHSAEILSLPHELSRLEVS-------SSTAL---KPTSAPQTS--------------- 406
            :.:..:|..:|..:|   |||.|..|       |:|.|   .|.|:|.|:
  Rat   331 VLISSSNPTAAARIS---ELSGLSCSAPSQVHISTTGLIVTPPPSSPVTTGPSFTFPTDVPYQAA 392

  Fly   407 -----------RVLKTTV---------------------------------------QQQMQPNL 421
                       .:|.|||                                       :.|.:|::
  Rat   393 LGTMNPPLPPPPLLATTVLASTPSGATAAAVAAAAAAVAAGVGPRPAVGSTEQIAHLRPQTRPSV 457

  Fly   422 PWGYLALFPYKPRQTDELELKKGCVYIVTERCVDGWFKGKNW-LDITGVFPGNYLTPL-RARDQQ 484
               |:|::||.||:.|||||:||.:::|.|||.|||:||.:. ....|||||||:.|: ||
  Rat   458 ---YVAIYPYTPRKEDELELRKGEMFLVFERCQDGWYKGTSMHTSKIGVFPGNYVAPVTRA---- 515

  Fly   485 QLMHQWKYVPQNADAQMAQVQQHPVAPDVRLNNMLSMQPPDLPPRQQQATATTTSCSVWSKPVEA 549
                       ..:|..|:|.........|...|:|......|.::.|......:.||
  Rat   516 -----------VTNASQAKVPMSTAGQASRGVTMVSPSTAGGPAQKPQGNGVAGNPSV------- 562

  Fly   550 LFSRKSEPKPETATASTTSSSSSGAVGLMRRLTHMKTRSKSPGASLQQVPKEAIST----NVEFT 610
                     ..||..|.....:|....::..:|...|.:::         :.|:.|    |.|
  Rat   563 ---------VPTAVVSAAHIQTSPQAKVLLHMTGQMTVNQA---------RNAVRTVAAHNQE-- 607

  Fly   611 TNPSAKLHPVHVRSGSC--------PSQLQHSQPL---------------NET------------ 640
             .|:|.:.|:.|::.:|        |.....||||               |.|
  Rat   608 -RPTAAVTPIQVQNAACIGPASVGLPHHSLASQPLPPMVGPAAHIAAVNINRTSVPLACAAGASS 671

  Fly   641 ---PAAKTAAQQQQ-------FLPKQLPSA--STNSVSYGSQRVK----GSKERPHLICARQSLD 689
               |...|||.:.:       .|| .||::  |..|....|..||    ..||:..|:   :.|.
  Rat   672 LASPNMTTAALETEPSGRTVTILP-GLPTSPESAASACGNSSAVKPDKDSKKEKKGLL---KLLS 732

  Fly   690 AATFRSMYNNAASPP-PPTTSV------APAVYAGGQQQVIPGGGAQSQLHANMIIAPS------ 741
            .|:.:.  ....||| .||..|      .|...|.|.:  :|.||    :|..:...|:
  Rat   733 GASTKR--KPRVSPPASPTLDVELGSGEVPLQGAVGPE--LPLGG----VHGRVGSCPTDGDGPV 789

  Fly   742 ------------HRKSHSLDASHVLSPSSNMITEAAIKASATTKSPYCTRESRFRCIVPYPPNSD 794
                        |||:.|||::..::|.......:........:...|   .|.|.:|.|||.|:
  Rat   790 AAGTAALAQDAFHRKTSSLDSAVPIAPPPRQACSSLGPVMNEARPVVC---ERHRVVVSYPPQSE 851

  Fly   795 IELELHLGDIIYVQRKQKNGWYKGTHARTHKTGLFPASFVE 835
            .||||..|||::|.:|:::||:|||..|..||||||.||||
  Rat   852 AELELKEGDIVFVHKKREDGWFKGTLQRNGKTGLFPGSFVE 892

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
POSH	NP_523776.1	RAD18	6..>113	CDD:227719	60/127 (47%)
		RING-HC_SH3RF3	8..53	CDD:438408	32/44 (73%)
		SH3	139..191	CDD:473055	23/51 (45%)
		SH3_SH3RF_2	199..251	CDD:212721	27/52 (52%)
		SH3_SH3RF_3	424..477	CDD:212717	29/53 (55%)
		SH3_SH3RF_C	782..836	CDD:212719	32/54 (59%)
Sh3rf1	NP_942059.1	RING-HC_SH3RF1	8..55	CDD:438406	34/46 (74%)
		SH3	137..190	CDD:473055	24/52 (46%)
		SH3_SH3RF1_2	200..254	CDD:212863	27/53 (51%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	274..323		9/65 (14%)
		Interaction with RAC1. /evidence=ECO:0000250|UniProtKB:Q7Z6J0	293..363		17/88 (19%)
		Interaction with AKT2. /evidence=ECO:0000250	448..551		41/120 (34%)
		SH3	457..511	CDD:473055	29/56 (52%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	526..556		5/29 (17%)
		PHA03247	<541..854	CDD:223021	73/355 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	682..751		19/74 (26%)
		SH3_SH3RF_C	839..893	CDD:212719	32/54 (59%)