Protein Alignment POSH and Sh3rf3

DIOPT Version :10

• Sequence 1: NP_523776.1
• Gene: POSH / 36990
• FlyBaseID: FBgn0040294
• Length: 838
• Species: Drosophila melanogaster
• Sequence 2: NP_766376.2
• Gene: Sh3rf3 / 237353
• MGIID: 2444637
• Length: 878
• Species: Mus musculus
• Alignment Length: 925
• Identity: 300/925 - (32%)
• Similarity: 431/925 - (46%)
• Gaps: 179/925 - (19%)

  Fly     1 MDEHTLNDLLECSVCLERLDTTSKVLPCQHTFCRKCLQDIVASQHKLRCPECRILVSCKIDELPP 65
            |||.:|.|||||||||||||||:|||||||||||:||:.||.|:|:||||||||||.|.:||||.
Mouse    41 MDESSLLDLLECSVCLERLDTTAKVLPCQHTFCRRCLESIVCSRHELRCPECRILVGCGVDELPA 105

  Fly    66 NVLLMRILEGMKQNAAAGKGEEKGEETETQPERAKP---------------QPPAESV------A 109
            |:||:|:|:|::|....|     .....:.|.|..|               .||...|      .
Mouse   106 NILLVRLLDGIRQRPRTG-----ASPGSSPPARPGPGTFSALAGGAGGATGSPPCSPVFLSAAAG 165

  Fly   110 PPDNQLLQLQSHQQSHQPARHKQRRFLLPHAYALFDFASGEATDLKFKKGDLILIKHRIDNNWFV 174
            ...:.|..:.:::.........|    ||:|.||:.:...|..||||.|||:|:::.::|.||:.
Mouse   166 SSTSSLCDVATNRSVPVAKTLSQ----LPYAKALYSYEGKEPGDLKFNKGDIIILRRKVDENWYH 226

  Fly   175 GQANGQEGTFPINYVKVSVPLP--MPQCIAMYDFKMGPNDEE-GCLEFKKSTVIQVMRRVDHNWA 236
            |:..|..|..|.:|::...|||  :||..|:|||:|...|:: .||.|.|..|:.|:||||.|||
Mouse   227 GELQGMHGFLPASYIQCVRPLPQALPQGKALYDFEMKDRDQDKDCLTFTKDEVLTVIRRVDDNWA 291

  Fly   237 EGRIGQTIGIFPIAFVELNAAAKKLLDSGLHTHPFCHPPKQQGQRALPPVPVIDPTVVTESSSGS 301
            ||.:|..|||||:.:||||.:||:|::    ....|  |..........:...||:.|...:.|.
Mouse   292 EGMLGDKIGIFPLLYVELNDSAKQLIE----MDKLC--PAATTAYNYDALLSSDPSTVASVAPGP 350

  Fly   302 SNSTPG--SSNSSSTSSSNNCSPNHQISLPNTPQHVVASGSASVRFRDKGAKEKRHSLNALLGGG 364
            :.|:.|  |:......|..|....|..:            :.||..:...|...|||:..    .
Mouse   351 TLSSSGAVSAFQRRVDSKKNAKKRHSFT------------ALSVTHKSSQAASHRHSMEI----S 399

  Fly   365 APLSLLQTN-RHSAEILSLPHELS---RLEVSSSTALKPTSAPQTSRVLKTTVQQQMQP--NLPW 423
            ||:.:..:: |.:|.|..|.| ||   ..:.|||....||:.|:.:.|   ..:|.|.|  .||.
Mouse   400 APVLISSSDPRAAARIGELAH-LSCTVPTQDSSSAGPVPTALPRAAAV---AGEQGMSPKVQLPL 460

  Fly   424 G-YLALFPYKPRQTDELELKKGCVYIVTERCVDGWFKGKNW-LDITGVFPGNYLTPLR------- 479
            . ||||:.|||::.|||||:||.:|.|.|:|.||||||.:. ..::|||||||:||:.
Mouse   461 NVYLALYAYKPQKNDELELRKGEMYRVLEKCQDGWFKGASLKTGVSGVFPGNYVTPVSRVPGGGA 525

  Fly   480 -------------ARDQQQLMHQWKYVPQNADAQMAQVQQHPVAPDVRLNNMLSMQPPDLP---- 527
                         |:....:||     |................|...|.:.:......||
Mouse   526 GLPWNNVLGGSPLAKGMATIMH-----PGGGSLSSPATAARSALPLTTLQDHMQHPATSLPTGSC 585

  Fly   528 ------PRQQQA------TATTTSCSVWSKPVEALFSRKSEPKPETATAS----TTSSSSSGAVG 576
                  |...||      |||..|.....:|              |||.|    .||.|...:.|
Mouse   586 LRHSAQPTASQAGDTTIPTATHASAQALDRP--------------TATVSPLRTQTSPSRLPSTG 636

  Fly   577 LM-RRLTHMKTRSKSPGASLQQVPKEAISTNVEFTTNPSAKLHPVHVRSGSCPSQLQHSQPLN-- 638
            |. |.:...:...:||.   |..|:.||    .||:..|| :.|.:|.:.:...:: ...|::
Mouse   637 LRPRSVASPQHGQQSPA---QMCPRPAI----PFTSAASA-ITPPNVSAANLSGEV-GGTPISGL 692

  Fly   639 ETPA-----------AKTAAQQQQFLPKQLPSASTNSVSYGSQRVKGSKERPHLICARQSLDAAT 692
            .||:           .....:::..|.|.|..|||...|.....|..:.:      .:.::|.:
Mouse   693 STPSLINTGFKPDDKKNEKKEKKSGLLKLLAGASTKKKSRSPPSVSPTHD------PQSAMDTS- 750

  Fly   693 FRSMYNNAASPPPPTTSVAPAVYAG--GQQQVIPGGGAQSQLHANMIIAPSHRKSHSLDASHVLS 755
                ...|..|     .|:|....|  |...:      :|::...:.:.|.|||:.|||.:..||
Mouse   751 ----LQGAMGP-----EVSPLTVHGRAGSCPI------ESEMQGAIGLEPLHRKAGSLDLNFSLS 800

  Fly   756 PSSNMITEAAIKASATTKSPYCTRESRFRCIVPYPPNSDIELELHLGDIIYVQRKQKNGWYKGTH 820
            ||    .:|.:..::....|......|:|.:|.|||.|:.|:||..|||::|.:|.::||:|||.
Mouse   801 PS----RQATLSMASIRPEPKPLPRERYRVVVSYPPQSEAEIELKEGDIVFVHKKHEDGWFKGTL 861

  Fly   821 ARTHKTGLFPASFVE 835
            .|..:|||||.||||
Mouse   862 QRNGRTGLFPGSFVE 876

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
POSH	NP_523776.1	RAD18	6..>113	CDD:227719	62/127 (49%)
		RING-HC_SH3RF3	8..53	CDD:438408	37/44 (84%)
		SH3	139..191	CDD:473055	20/51 (39%)
		SH3_SH3RF_2	199..251	CDD:212721	28/52 (54%)
		SH3_SH3RF_3	424..477	CDD:212717	31/54 (57%)
		SH3_SH3RF_C	782..836	CDD:212719	30/54 (56%)
Sh3rf3	NP_766376.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	19..40
		RING-HC_SH3RF3	48..93	CDD:438408	37/44 (84%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	120..145		4/29 (14%)
		SH3	190..243	CDD:473055	21/52 (40%)
		SH3	253..307	CDD:473055	28/53 (53%)
		Interaction with RAC1. /evidence=ECO:0000250|UniProtKB:Q8TEJ3	364..433		20/85 (24%)
		SH3_SH3RF3_3	461..517	CDD:212858	32/55 (58%)
		Herpes_BLLF1	<543..>818	CDD:282904	69/328 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	574..659		24/101 (24%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	688..758		14/85 (16%)
		SH3_SH3RF_C	823..877	CDD:212719	30/54 (56%)