DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment CG4853 and RASGRF2

DIOPT Version :9

Sequence 1:	NP_611222.2	Gene:	CG4853 / 36974	FlyBaseID:	FBgn0034230	Length:	709	Species:	Drosophila melanogaster
Sequence 2:	NP_008840.1	Gene:	RASGRF2 / 5924	HGNCID:	9876	Length:	1237	Species:	Homo sapiens

Alignment Length:	600	Identity:	145/600 - (24%)
Similarity:	228/600 - (38%)	Gaps:	149/600 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly   133 PVKQQSALARATPEN----ALKYRTTSNSTFASQATDSDERPVDPLGRQLNQTFSNASLMSSSSA 193
            ||:.:. |:..:|.|    ||...|:|:.|..:|:..:...|           .:....:..|..
Human   739 PVRARK-LSLTSPLNSKIGALDLTTSSSPTTTTQSPAASPPP-----------HTGQIPLDLSRG 791

  Fly   194 ITGSDQVIGSVSSTTIGDKSSNISSCDSGHDGLFHSVSMSAP------WSHPRDTLTDSLYCDIP 252
            ::..:|..|:|....   .:..:..|:.....:..:|..|||      .|.|....|:...|..|
Human   792 LSSPEQSPGTVEENV---DNPRVDLCNKLKRSIQKAVLESAPADRAGVESSPAADTTELSPCRSP 853

  Fly   253 SSADESDAVSILNTHLKITEP-------FDCQTAPVYAKDI----------PKKGNLDSVVNLLI 300
            |:.          .||:..:|       ..|..:|..|..|          |...|.:
Human   854 STP----------RHLRYRQPGGQTADNAHCSVSPASAFAIATAAAGHGSPPGFNNTE------- 901

  Fly   301 NNNTKDLRFAF--LTTSRLFSPPHQLLSKIIA--KIDNE-EENVLRLLNEWLDTCPGDFSHELLL 360
              .|.|..|..  ..|:|:.:.....:||...  :::|| :.|||.||.|.|.      ..:||.
Human   902 --RTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLR------DPDLLP 958

  Fly   361 QELENKRNVKGLAAFLDGIPQALAQKDENG-RSQLNNLLTKQSSASSLGRQSSIKSLKRFAANVY 424
            ||.:...|          |.:||:|.|::. ..:|.::                         :.
Human   959 QERKAAAN----------ILRALSQDDQDDIHLKLEDI-------------------------IQ 988

  Fly   425 KTDNVLNNC---SSAFELAHQLYAIEYAYLSQIRLEEFV----EILEKDELKTCISQTKAGTLGS 482
            .||.:...|   .||.|||.|:..:::.....|..|||:    ..|:|:|....|.:|.
Human   989 MTDCMKAECFESLSAMELAEQITLLDHVIFRSIPYEEFLGQGWMKLDKNERTPYIMKTS------ 1047

  Fly   483 NCISQVTIDSYVQWFNQLSYLTATEILKLGKKSQRAQMIDFWVETALECFNTGNFNSLMAILTAL 547
                        |.||.:|.|.|::|:.....|.||..|:.||..|..|....|:|.::.|.:||
Human  1048 ------------QHFNDMSNLVASQIMNYADVSSRANAIEKWVAVADICRCLHNYNGVLEITSAL 1100

  Fly   548 NLTAIARLKKTWAKV-QTTK--FEGLEHQMDPSSNFLNYRSTMKAAVWRSEREMANEIERAIIPF 609
            |.:||.||||||||| :.||  .:.|:..:.....|.|.|.|:|            ......:|:
Human  1101 NRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLK------------NCNPPAVPY 1153

  Fly   610 FSLFLKDLHAINESHETKLANGYINFEKCLHLGTQLRNFGKWQRLDCPYEQLPSVVSYLL-KAEV 673
            ..::|.||..|.|........|.:||.|...:...:|...::|:.....:..|.|..||| |..:
Human  1154 LGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTSYRIDHQPKVAQYLLDKDLI 1218

  Fly   674 LSEDLLMKASYESEP 688
            :.||.|.:.|.:.||
Human  1219 IDEDTLYELSLKIEP 1233

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG4853	NP_611222.2	RasGEF_N	288..365	CDD:279012	21/81 (26%)
CG4853	NP_611222.2	RasGEF	438..638	CDD:279011	62/206 (30%)
RASGRF2	NP_008840.1	PH_RasGRF1_2	19..158	CDD:270081
		RhoGEF	247..428	CDD:214619
		PH	480..588	CDD:214574
		RasGEF_N	638..>686	CDD:306971
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	710..809		16/84 (19%)
		Regulates proteasomal degradation. /evidence=ECO:0000250	744..752		2/8 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	829..873		11/53 (21%)
		RasGEFN	<901..972	CDD:214571	23/95 (24%)
		RasGEF	998..1234	CDD:214539	78/265 (29%)
		Responsible of the affinity for farnesylated versus geranylgeranylated Ras. /evidence=ECO:0000250	1099..1128		17/28 (61%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.910