DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CG4853 and RASGRF1

DIOPT Version :9

Sequence 1:	NP_611222.2	Gene:	CG4853 / 36974	FlyBaseID:	FBgn0034230	Length:	709	Species:	Drosophila melanogaster
Sequence 2:	NP_002882.3	Gene:	RASGRF1 / 5923	HGNCID:	9875	Length:	1273	Species:	Homo sapiens

Alignment Length:	597	Identity:	141/597 - (23%)
Similarity:	243/597 - (40%)	Gaps:	129/597 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly   168 ERPVDPLGRQLNQTFSNASLMSSSSAITGSDQVIGSVSSTTI-GDKSSNIS--SCDS-GHDGLFH 228
            |.|..|   :..:.||:...:|.:...:.|.:...|::...| |.|:.:::  ||:| |:..::.
Human   726 EPPKSP---RATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYS 787

  Fly   229 SVSMSAPWSHPRDTL-TDSLYCD------IPSSAD-------ESDAVSILNTHLKITEPF----- 274
            ::|   |:|  :.|| |..||..      ||...|       :..|:|..::.:.:.|..
Human   788 AMS---PFS--KATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSALSKQSSEVSMREESDIDQN 847

  Fly   275 -----DCQTAPVYAKDIPK--KGNLDSVVNLL-------------INNNTKDL----RFAFLTTS 315
                 |.:|:|..:...||  |....|...|.             ::||...|    .||..|..
Human   848 QSDDGDTETSPTKSPTTPKSVKNKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAG 912

  Fly   316 RLFSPP----HQLLSKIIAKIDNEEEN--------------VLRLLNEWLDTCPGDFSHELLLQE 362
            .....|    ::.:|...|....::.|              ||.:|..|:.....||...   .|
Human   913 ANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETN---DE 974

  Fly   363 LENKRNVKGLAAFLDGI---PQALAQKDENGRSQLNNLLTKQSSASSLGRQSSIKSLKRFAANVY 424
            |:.|     :..||:.:   |:.|.| :....:.:...||::....:   |.:::.:.:.|..|
Human   975 LKCK-----VIGFLEEVMHDPELLTQ-ERKAAANIIRTLTQEDPGDN---QITLEEITQMAEGV- 1029

  Fly   425 KTDNVLNNCSSAFELAHQLYAIEYAYLSQIRLEEFV----EILEKDELKTCISQTKAGTLGSNCI 485
            |.:...|:  ||.|:|.||..:::....:|..|||.    ..|||:|....|.:|
Human  1030 KAEPFENH--SALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKT---------- 1082

  Fly   486 SQVTIDSYVQWFNQLSYLTATEILKLGKKSQRAQMIDFWVETALECFNTGNFNSLMAILTALNLT 550
                    .:.||.:|.|.|:||::....:.|...|:.||..|..|....|:|:::.|.:::|.:
Human  1083 --------TKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRS 1139

  Fly   551 AIARLKKTWAKV-QTTK--FEGLEHQMDPSSNFLNYRSTMKAAVWRSEREMANEIERAIIPFFSL 612
            ||.||||||.|| :.||  .:.|:..:.....|.|.|..:|            ..:...:|:..:
Human  1140 AIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALK------------NCDPPCVPYLGM 1192

  Fly   613 FLKDLHAINESHETKLANGYINFEKCLHLGTQLRNFGKWQRLDCPYEQLPSVVSYLL-KAEVLSE 676
            :|.||..|.|.......:|.:||.|...:...:|...::|:.....|....|..||| ::.|:.|
Human  1193 YLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDE 1257

  Fly   677 DLLMKASYESEP 688
            :.|.::|...||
Human  1258 ESLYESSLRIEP 1269

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG4853	NP_611222.2	RasGEF_N	288..365	CDD:279012	22/113 (19%)
CG4853	NP_611222.2	RasGEF	438..638	CDD:279011	57/206 (28%)
RASGRF1	NP_002882.3	PH	489..583	CDD:278594
		RasGEF_N	647..>695	CDD:279012
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	724..754		7/30 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	809..874		12/64 (19%)
		REM	<938..1007	CDD:295342	15/77 (19%)
		RasGEF	1034..1270	CDD:214539	74/268 (28%)
		PH_RasGRF1_2	19..154	CDD:270081
		PH	24..129	CDD:278594
		RhoGEF	244..425	CDD:214619
		PH-like	431..582	CDD:302622
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.910