Protein Alignment CG4853 and efc25

DIOPT Version :9

• Sequence 1: NP_611222.2
• Gene: CG4853 / 36974
• FlyBaseID: FBgn0034230
• Length: 709
• Species: Drosophila melanogaster
• Sequence 2: NP_596123.1
• Gene: efc25 / 2540248
• PomBaseID: SPBC336.03
• Length: 987
• Species: Schizosaccharomyces pombe
• Alignment Length: 725
• Identity: 144/725 - (19%)
• Similarity: 264/725 - (36%)
• Gaps: 197/725 - (27%)

  Fly     3 EDILNAVES-----LFGKDVKIVDCETSSSLQQEEVLLVNGVPVKLDGLPADDASAIKAALLEGQ 62
            |.|.|.:||     .|.||:.  :||.:::...:...:.......|          :...:...|
pombe   418 EQIFNKLESWDMSIYFDKDLS--ECEQATNFAVQSYFVTKQRCYDL----------LTDLVCSSQ 470

  Fly    63 MPSVEHLNQLLFRAGLAQQPIEVETSLTVKSSLTTTEEVLVSRNGQILDERTVETKENFNHQSHQ 127
            ...:||.|             :..|..|:.:|:....:.|.               ||
pombe   471 DLMMEHSN-------------DFSTMPTMIASIAVAFQTLF---------------EN------- 500

  Fly   128 KEIWHPVKQQSALARATPENALKYRTTSNSTFASQATDSDERPVDPLGRQLNQTFSNASLMSSSS 192
              :...:|.::||.....|.|.|   ...:.|::..|..|:.|    .||.|:
pombe   501 --VCDFLKVRAALVDEMQELATK---EFENKFSNANTAKDDEP----ARQTNK------------ 544

  Fly   193 AITGSDQVIGSVSSTTIGDKSSNISSCDSGHDGLFHSVSMSAPWSHPRDTLTDSLYCDIPSSADE 257
               |:.::..|...|.:.:.|                          :||||          ...
pombe   545 ---GTTRISRSSDFTAVSEMS--------------------------KDTLT----------LGR 570

  Fly   258 SDAVSILNTHLKITEPFDCQTAPVYAKDIPKKGNLDSVVNLLINN--NTKDLRFAFLTTSRLFSP 320
            :...|||.....:|...      |.:.:..|.|.|.::|:.|:.|  ..||.|.:||.|.:.|:.
pombe   571 NSLQSILMLDNLLTNKV------VQSDNNVKGGTLPALVHYLVQNVHLNKDFRHSFLLTYKTFTT 629

  Fly   321 PHQLLSKII-------------------AKIDN--EEENVLRLLNEWLDTCPGDFSHELLLQELE 364
            |.:|.:.::                   .|.||  .::||..::|.|:        .:...::|:
pombe   630 PQELFTLLVILFHELPPPGLDATAYSSWEKGDNFVTKKNVCTVMNLWV--------QKYFFEDLK 686

  Fly   365 NKRNVKGLAAFLDGIPQALAQKDENGR---SQLNNLLTKQSSASSLGRQSSIKSLKRFAANVYKT 426
            .:..:..::.....:...:......|.   |:::||.|::...|...|..|.......:.|||
pombe   687 ARNTLYLISEMRTFLRDHVVPSFHIGSVILSEIDNLWTEEPPDSLTQRLLSSPMATFISLNVY-- 749

  Fly   427 DNVLNNCSSAFELAHQLYAIEYAYLSQIRLEEFVEILEKDELKTCISQTKAGTLGSNCISQVTID 491
                  ..:..|.|.|:..:|:.||.||...|::       .::.:|:.          |:..:.
pombe   750 ------AYTPEEFASQMTLLEFDYLKQIPSREWI-------FRSWVSRD----------SRSAVR 791

  Fly   492 SYVQWFNQLSYLTATEILKLGKKSQRAQMIDFWVETALECFNTGNFNSLMAILTALNLTAIARLK 556
            :|:.:.|..:|.....||:......|..:|.|:::||.:|.:..||::||:|::|||...|.||.
pombe   792 NYINFSNCFTYWIINCILEKKNTKARTAVISFFIQTAYKCLSLQNFSTLMSIVSALNSAPIYRLH 856

  Fly   557 KTWAKVQTTK---FEGLEHQMDPSSNFLNYRSTMKAAVWRSEREMANEIERAIIPFFSLFLKDLH 618
            ..:..|:...   ..||...::...||..||:.::.|            |...:||..:.|.||.
pombe   857 AAYKLVKAEDIICLSGLREIVETKKNFSTYRALLRKA------------ELPCVPFLGVILSDLT 909

  Fly   619 AINESHETKLANG--YINFEKCLHLGTQLRNFGKWQRLDCPYEQLPSVVSYLL-KAEVLSEDL-- 678
            .|:|.:...|.:.  .::|.|...|...:.:..::|......:....:.||:| :...:::||
pombe   910 FIDEGNPDVLDSSPHLLSFNKRHRLADVVADVCRFQSSSYEMQSNTDLQSYILHRCRFVNQDLSY 974

  Fly   679 LMKASYESEP 688
            |...|...||
pombe   975 LFDKSLSLEP 984

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CG4853	NP_611222.2	RasGEF_N	288..365	CDD:279012	23/99 (23%)
		RasGEF	438..638	CDD:279011	51/204 (25%)
efc25	NP_596123.1	RasGEF_N	593..700	CDD:279012	23/114 (20%)
		RasGEF	755..932	CDD:279011	52/205 (25%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	2	1.870