Protein Alignment RhoGEF2 and Akap13

DIOPT Version :10

• Sequence 1: NP_477317.1
• Gene: RhoGEF2 / 36915
• FlyBaseID: FBgn0023172
• Length: 2559
• Species: Drosophila melanogaster
• Sequence 2: XP_036009413.1
• Gene: Akap13 / 75547
• MGIID: 2676556
• Length: 2817
• Species: Mus musculus
• Alignment Length: 2700
• Identity: 528/2700 - (19%)
• Similarity: 905/2700 - (33%)
• Gaps: 839/2700 - (31%)

  Fly    11 LDLYTDEHEYDEVQEIPEESSIQP------------------PETSTSH-----TSTNGSSHSGP 52
            |||.......|.:.|...||..:|                  |....:|     ||:......|.
Mouse   615 LDLTMPGPSSDGMPEQNSESHARPAQSLSGQALLCSTAEAGTPSAEATHQPSTVTSSGRLEECGS 679

  Fly    53 GTATGPGATSAGPSAGA---------PQSPVIVVDSVPELPAPKQKSV----------KNSKSKQ 98
            |.|:.|.:|...||...         ||:..:..|:|..    .||||          ||..:..
Mouse   680 GKASLPESTMVQPSTQELCTTLCPEDPQADTVTSDTVKN----TQKSVGVCHLCVSDAKNQGNGL 740

  Fly    99 KQ-KQLAN-KSKIPRSPSLAS---------------SLSSLASSLSGHRDRDKDRDKDRENQNAV 146
            || ..|.| ...:||.||:.|               |..|||||              .|:::..
Mouse   741 KQDTPLTNVLEDVPRLPSVVSQTEKELAPDQVSPPASSFSLASS--------------PESESVT 791

  Fly   147 PPQTPPLPPSYKQNQMNGDSTAAAGGGVSAPATPTTANNNNASHNNGSIMGGGVQLNQSDNSNPV 211
            ......|.||.|:               ...|||..       |...:...|
Mouse   792 KDDALSLVPSQKE---------------KGTATPQL-------HRTTACRDG------------- 821

  Fly   212 LQAPGERSSLNLTPLSRDLSGGHTQESTTPATTPSTPSLALPKNFQYLTLTVRKDSNGYGMKVSG 276
                         |..||||  .|.:....||.| .||.|:.                  ::.|.
Mouse   822 -------------PDGRDLS--DTDKVGDGATDP-PPSSAVE------------------LRTSM 852

  Fly   277 DN--PVFVESVKPGGAAEIAGLVAGDMILRVNGHEVRLEKHPTVVGLIKASTTVELAVKRSQKLT 339
            .|  ||.:...:.|.:......|..|.:|    |.|  :|    ..|:...|..|
Mouse   853 GNTSPVGIGGEQEGSSPTATLEVLSDSLL----HNV--DK----AALVSDFTLPE---------- 897

  Fly   340 RPSSVSVVTP--STPI-LSGRDRTASITGPQPVDSIKRREMETYKIQT--------------LQK 387
              ..||||.|  ||.: ..|:||..|.:..:  :.:...||.....:|              :..
Mouse   898 --EGVSVVVPESSTALGQDGKDRAMSCSSVK--EDVHSSEMSREDQRTPPSGQEIPGLCEKPMSA 958

  Fly   388 MLEQEKLNLERLKSDQNNPS--YKLSEANIRKLREQLHQVGAEDAPTVKLQAAAGNKNTALLTPN 450
            :..:|       |:.|:.||  .|....:|:           |.||.|.| ...|....:|:.|.
Mouse   959 LCAEE-------KAQQHTPSACLKTETKDIK-----------EVAPQVSL-LTEGGAAKSLVPPR 1004

  Fly   451 QIQHLSASATHSNQQFHHLHHHHNLHNNNYPPQQQPASTSPAFLSLLPRSLSSLSLGTRKNKTEK 515
              ..|||.:                       :|:.:||..:..||||..|...|.....|:
Mouse  1005 --TSLSADS-----------------------KQKASSTEQSGSSLLPSGLPGASEALHCNQ--- 1041

  Fly   516 DLTTSSPFGLTTDFLQQQRMSHQAESMSQSMHQHTSTPTSQQFFHPHQQ--QHRFKETGPTSKGK 578
                .|...:..:..|.|..::..|....:|...|....|.....|.::  .|..|:. |.|:..
Mouse  1042 ----PSALDVVVENTQFQGETNACEVSRSAMEDVTVADASPATAEPRKKDASHCIKDI-PISELL 1101

  Fly   579 NKFLISRSLIEEDVPPPLPQRNPPRQLNLDLKNGNASPGGSHLVAPVSDLDRATSPQLNRSQ-QQ 642
            |:        |:.:.|.||:                    :.|...|:||....:|::.... ::
Mouse  1102 NQ--------EKQMTPSLPE--------------------AFLDKGVTDLQEVITPEIEPLDCKR 1138

  Fly   643 QLPSSTDNSPSNAKSKRSKIKTKALSDPKMSTQMFLQMESASAAGAAGGSIEVDGGPPPLPPRLP 707
            :....||.:.:.:.||.:.|: |.:.                                ||...||
Mouse  1139 ETLEGTDLNCATSNSKETPIE-KPMQ--------------------------------PLARDLP 1170

  Fly   708 -----GMMTEDMSRGSCQNLAQPNSVGTAFNYPLVSTTTA-----------------------VQ 744
                 .::..::.:...:.:||.:......|...|||..|                       |:
Mouse  1171 TETGLSVINNNVPQADMKQVAQASIPAEESNATTVSTQAADVPTRADSIEETATRIVEAVIRQVR 1235

  Fly   745 NDNLNIAFPLSQRPNIVQQLQQYQQQQQHQMSGGQATGAL----GQTPNLGKNKHRRVG------ 799
            ..|..:|...:|.|::       ...:..|:.......|.    |:||.:.|......|
Mouse  1236 ASNALMAKVETQNPSL-------SSPETKQLENAYTESACAFLPGETPQIEKTHEDTTGQCGAET 1293

  Fly   800 -------------SSPDNMHPRHPDRITKTTSGSWEIVEKDGESSP-----PGTPPPPYLSSSHM 846
                         ..||.......|.:::.::.|.|....:|.::|     |||      .::..
Mouse  1294 EEPEKIILPESAPEMPDTRTGDEVDLLSRISAASEEEAVGNGAATPKMKQGPGT------QANDC 1352

  Fly   847 T-----VLEDPNENNRGAAAAGPGVFIESHQFTPMAGASSPIPISLHSSHMHAAQSNDTQK---- 902
            |     ..|:.|:.|:    :.|.:..||.      .|..|.|.:  :|.:.:.||::.:.
Mouse  1353 TRVIPSCCEEKNQRNK----SSPKINRESW------CAIEPCPEA--ASLLASKQSSECRSFIDV 1405

  Fly   903 ----EIISME-------DENSDLDEPFIDENGPFNNLTRLL--EAENVTFLAIFLNYVISNSDPA 954
                |..|.|       ...|||      .:.|.:.:..::  :.|....|........|..|.|
Mouse  1406 GLGTECASKEGMLQRVSGSESDL------FHSPSDEMDSIIFPKPEEEQLLCDTTGSSSSTDDTA 1464

  Fly   955 PLLFYLI--TELYKEGTSKDMRKWAYEIHSTFLVP--RAPLSWYRQDESLAREVDNVLQLEYDKV 1015
            .|..:..  :::....|||..|...::..:.|..|  .||.|  |:.||   |.....::|.:::
Mouse  1465 SLDRHSSHGSDVSLPQTSKLNRSRNHQSANGFFSPGVEAPES--RESES---EPAGSGEMEEEEM 1524

  Fly  1016 EILRTV-----FLRSRKRAKDLISEQLREFQQKRTAGLGTIYGPTDDKLAEAKTDKLREQIIDKY 1075
            :.:..|     ||||..|:       |..|::..       :||..:..::|:.::........:
Mouse  1525 DSITEVPANCSFLRSSMRS-------LSPFRRHS-------WGPGKNAASDAEMNQRSSMRALGH 1575

  Fly  1076 LM--PNLH---------------ALIEDEN--------------GSPPEDVRKVALCSALSTVIY 1109
            ::  |.:|               ||..|.|              |:.|..  .:.:.||.|:.:.
Mouse  1576 VVRRPPIHRRSMSWCPSGVQYSAALNADFNIRSFSLEGLTGGGVGNKPSS--SLEMSSANSSELR 1638

  Fly  1110 RIFNTRPPPSSIV----------ERVHH-FVSRDKSFKSRIMGKNRKMNVRGHPLVLR-QYYEVT 1162
            ..|......:|::          :|.|| ...:...::|:..|.|...:....||... ....::
Mouse  1639 NPFGGEEQRNSLMSLSEEHLEPDQRQHHRMFDQQTCYRSKQQGFNYCTSAISSPLTKSISLMTIS 1703

  Fly  1163 HCNHCQTIIWGV-SPQGYHCTDCKLNIHRQCSKVVDESC---PGPLPQAKRLAHND--KISKFMG 1221
            |.        |: |.:.:|.|...|.     ..:.:|:|   | |.|..|......  |:|:...
Mouse  1704 HP--------GLDSSRPFHSTSANLT-----ESITEENCNFLP-PSPSKKNFEEKSGTKVSRTFS 1754

  Fly  1222 KIRPRTSDVIGNEKRSRQDEELDVELTPDRGQASIVRQPSDRRPDANISIRSNGNT--------- 1277
            .||.:.|    :.|:|::.|         :.:..|..:..|.:.........||:|
Mouse  1755 YIRNKMS----SSKKSKEKE---------KEKDKIKEKEKDSKEKEKDKKTLNGHTFSPIPIVGP 1806

  Fly  1278 -SCNTSGLNTTDLQSSFHGSCANDSINPGGGA----GCNMDLSTSVASTTPSTSGSVAAGLSAFA 1337
             ||:......|:..:.   :||      |.||    ||..:|::..........||:.|     .
Mouse  1807 ISCSQCMKPFTNKDAY---TCA------GCGAFVHKGCRENLASCAKVKMKQPKGSLQA-----H 1857

  Fly  1338 ELNALDTVDKEARRERYSQHPKHK------------SAPVSVNRSESYKERLSNKRNRNSRRKTS 1390
            :.::|.||   ..|.:.|| ||.:            :||:..||.......||        :..|
Mouse  1858 DTSSLPTV---IMRNKSSQ-PKERPRSAVLLADEATAAPMFTNRRSQQSVSLS--------KSVS 1910

  Fly  1391 DPSLSSRPNDEQLD---LGLSNAT-------YVGSSNSSLSSAGGSESPSTSMEHFAAPGAAGGV 1445
            ..:::...|||.:.   ..||::|       .|..|..||:..|
Mouse  1911 IQNITGVGNDENMSNTWKFLSHSTDSLNKICKVNESTESLTDEG--------------------- 1954

  Fly  1446 QVPPMGLNQNQHPHLLIQQHAQQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPLPVARWTLESE 1510
                :|.:.|:                        |.......|:|.             .||:|
Mouse  1955 ----VGTDMNE------------------------GQLMGDFESDSK-------------QLEAE 1978

  Fly  1511 DEDDVNEADWSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYESGLLS 1575
                    .||..|.::.|........||||:|.|:.|||.:|:||||::..::...:....|..
Mouse  1979 --------SWSRTVDSKFLKQQKKDVVKRQEVIYELMQTELHHIRTLKIMSDVYSRGMMTDLLFE 2035

  Fly  1576 QDHLLLLFPPALLSLREIHGAFEQSLKQR-------RIEHNHVVNTIGDLLADMFDGQSGVVLCE 1633
            |..:..|| |.|..|..||..|.|.:.:|       :.|.|.::..|||:|...|.|:|...|.:
Mouse  2036 QQMVEKLF-PCLDELISIHSQFFQRILERKKESLVDKSEKNFLIKRIGDVLVSQFSGESAERLKK 2099

  Fly  1634 FAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLFENL 1698
            ...:||.:...::...|:...||:..|..:||..|....|||.:.:.:..|.||:||||:||:.:
Mouse  2100 TYGKFCGQHNQSVNYFKDLYTKDKRFQAFVKKKMSSSVVRRLGIPECILLVTQRITKYPVLFQRI 2164

  Fly  1699 YKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSSYDK----EE 1759
            .:.|    .:|..|.|.:.:::...|.::..|:..|.:.|...:|..|..|.|..|..:    :.
Mouse  2165 LQCT----KDNEVEQEDLTQSLSLVKDVIGAVDSKVASYEKKVRLGEIYTKTDSKSIMRMKSGQM 2225

  Fly  1760 FKKLDLTQHHLIHDGNLTIKKNPS--VQLHGLLFENMIVLLTKQDDKYYLKNL-HTPLSITNKPV 1821
            |.|.||.:..|:.||::.:|....  .::..:|..:::|.|.::|.||...:| |....|:.|.:
Mouse  2226 FAKEDLRRKKLVRDGSVFLKSTTGRLKEVQAVLLTDILVFLQEKDQKYVFASLDHKSTVISLKKL 2290

  Fly  1822 SPIMSIDADTLIRQEAADKNSFFLIKM--KTSQMLELRAPSSSECKTWFKHFSDVAARQSKNRSK 1884
                      ::|:.|.::...|||.|  |..:|:|:.|.|..|..:|.:...|.....:::..:
Mouse  2291 ----------IVREVAHEEKGLFLISMGVKDPEMVEVHASSREERNSWIQIIQDTINSLNRDEDE 2345

  Fly  1885 NASSNHDTSISDPALAAIPHSNTKESLE-------LSTDTVQPLAATATLTTTPLAPMLPIATVT 1942
            ...|.::   .:..|........||.|:       |..:..:.:....|..:||| |.....|.:
Mouse  2346 GIPSENE---EEKKLLDTKARELKEQLQQKDQQILLLLEEKEMIFRDMTECSTPL-PEDCSPTHS 2406

  Fly  1943 PAPATNNSNVSSLTGVQLRNPQRDATASESDADYVNTPKPRSSQNEVNRTMSI----------RS 1997
            |.....::...:|.|                     .|..:|:.|||....|:          :|
Mouse  2407 PRVLFRSNTEEALKG---------------------GPLMKSAINEVEILQSLVSGSLGGTLGQS 2450

  Fly  1998 TGEPIQK-------------YSANGTEANDVTLRHSQSTRESVRPGSTGEERNSTYG----MVGG 2045
            ...|:::             .:..|.:.:.:.........|    |..|::...|..    ..||
Mouse  2451 ISSPVEQEVMAAPISLPRRAETFGGFDCHQMNASKGGEKEE----GDDGQDLRRTESDSGLKKGG 2511

  Fly  2046 NS------KRDSASIVCSNNSNNTRTLLMQSPLVDPTAIQVSISPAHTAEPVLTPGEKLRRLDAS 2104
            |.      ||:|..:|.|....:....::|..::.        ..::..:..|...||:....||
Mouse  2512 NGNLVFMLKRNSEQVVQSIVHLHELLSMLQGVVLQ--------QDSYIEDQKLVLTEKVLTRSAS 2568

  Fly  2105 IRNDLLEKQKIICDIFRLPVEHYDQIVDIAMMPEAPKDSADIALAAYDQIQTL-TKMLNEYMHVT 2168
            ..:.|:|::|                     .....|...|:|.....|.|.| .|...|.....
Mouse  2569 RPSSLIEQEK---------------------QRSLEKQRQDLANLQKQQAQHLEEKRRREREWEA 2612

  Fly  2169 PEQEV-----------SAVSTAVCGHCHEKEKLRKKVAPSSSFSSSPPPLPPPNRQHAQAQAQIP 2222
            .|||:           ..|.........::|:|::|   ..::......|....:|..:.|.|:
Mouse  2613 REQELRDREAKLAEREETVRRRQQDLERDREELQQK---KGTYQCDLERLRAAQKQLEREQEQL- 2673

  Fly  2223 PSRLMPKLQTLDLDEVAIHEDDDGYCEIDE-----LRLPA-IPSKPHERPTTPLAPFNTEPKTSQ 2281
                     ..|.:.::..:.|...|::..     :|:|: :|:.  :..::|.||..|:..:..
Mouse  2674 ---------RRDTERLSQRQMDQNLCQVSNKHGRLMRIPSFLPNS--DEFSSPSAPSVTKSGSLD 2727

  Fly  2282 SVIDASKRQST------DAVPEGLLEQEPLEGDKTETKGEDNEVKTVPSDKLSE-----SCNEER 2335
            |.:..|.::::      |..|..:|      |..::||..:.:.:...|...|.     |..:|:
Mouse  2728 SELSVSPKRNSISRTQKDKGPFHIL------GSASQTKVPEGQSQAPSSTSTSTRLFGLSKPKEK 2786

  Fly  2336 QCVEADITKEVADPTTSKNEAAASVDELPSQSREI 2370
            :    :..|:.....|...:..||  |:|::..||
Mouse  2787 K----EKKKKSKGSRTQPGDGPAS--EVPAEGEEI 2815

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ_ARHGEF11-12-like	258..333	CDD:467282	14/76 (18%)
		RGS_GEF_like	934..1055	CDD:188710	29/129 (22%)
		C1_ARHGEF-like	1150..1202	CDD:410382	10/56 (18%)
		RhoGEF	1539..1731	CDD:238091	61/198 (31%)
		PH_16	1756..1876	CDD:436083	32/128 (25%)
Akap13	XP_036009413.1	C1_AKAP13	1792..1848	CDD:410428	14/64 (22%)
		RhoGEF	1999..2193	CDD:238091	61/198 (31%)
		PH_AKAP13	2236..2338	CDD:275427	28/111 (25%)
		YhaN	2530..>2706	CDD:443752	33/217 (15%)