Protein Alignment RhoGEF2 and arhgef12b

DIOPT Version :10

• Sequence 1: NP_477317.1
• Gene: RhoGEF2 / 36915
• FlyBaseID: FBgn0023172
• Length: 2559
• Species: Drosophila melanogaster
• Sequence 2: XP_009300160.2
• Gene: arhgef12b / 571031
• ZFINID: ZDB-GENE-070705-36
• Length: 1422
• Species: Danio rerio
• Alignment Length: 1963
• Identity: 407/1963 - (20%)
• Similarity: 653/1963 - (33%)
• Gaps: 774/1963 - (39%)

  Fly   193 GSIMGGGVQLNQSDNSNPVLQAPGERSSLNLTPLSRDLSGGHTQESTTPATTPSTPSLALPKNFQ 257
            |||:        |.:..|..:|..:::|:   |||.:..                     |....
Zfish    20 GSIL--------SKDHPPDKKAKSDKASV---PLSHEFD---------------------PTGLV 52

  Fly   258 YLTLTVRKDSNGYGMKVSGDNPVFVESVKPGGAAEIAGLVAGDMILRVNGHEVRLEKHPTVVGLI 322
            ...:.::||.||:|:.|||||||||:.||..|||..||:..||.|::|||..|....|..||.||
Zfish    53 QRCVIIQKDENGFGLTVSGDNPVFVQLVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHVEVVKLI 117

  Fly   323 KASTTVELAVKRSQKLTRPSSVSVVTPSTPILSGRDRTASITGPQPVDSIKRREMETYKIQTLQK 387
            |:.:.|.|.|     |.||..:    |..|:..|.
Zfish   118 KSGSYVALTV-----LGRPPGL----PQIPLSDGE------------------------------ 143

  Fly   388 MLEQEKLNLERLKSDQNNPSYKLSEANIRKLREQLHQVGAEDAPTVKLQAAAGNKNTALLTPNQI 452
                                                  |.|.:|
Zfish   144 --------------------------------------GEEGSP--------------------- 149

  Fly   453 QHLSASATHSNQQFHHLHHHHNLHNNNYPPQQQPASTSPAFLSLLPRSLSSLSLGTRKNKTEKDL 517
              .|.|:.||                       |....|      .||.||.|...||
Zfish   150 --FSLSSPHS-----------------------PGPIGP------ERSCSSPSPSERK------- 176

  Fly   518 TTSSP-FGLTTDFLQQQRMSHQAESMSQSMHQHTSTPTSQQFFHPHQQQHRFKETGPTSKGKNKF 581
            ..|.| :|:|.|:                                         ..||||
Zfish   177 AASLPVWGITEDY-----------------------------------------RNPTSK----- 195

  Fly   582 LISRSLIEE--DVPPPLPQRNPPRQLNLDLKNGNASPGGSHLVAPVSDLDRATSPQLNRSQQQQL 644
                 |::|  |:    ||.....||        :.|.|.         |.:.||:....:.:..
Zfish   196 -----LLKEIQDI----PQNQNQEQL--------SKPTGQ---------DESISPRPGEGELEDF 234

  Fly   645 PSSTDNSPSNAKSKRSKIKTKALSDPKMSTQMFLQMESASAAGAAGGSIEVDGGPPPLPPRLPGM 709
            ....|..|                                       |.:.|.|..||
Zfish   235 SGDVDRPP---------------------------------------SWQGDAGTEPL------- 253

  Fly   710 MTEDMSRGSCQNLAQPNSVGTAFNYPLVSTTTAVQNDNLNIAFPLSQRPNIVQQLQQYQQQQQHQ 774
                                             ..|:|:....|.
Zfish   254 ---------------------------------WTNNNMTPVTPC-------------------- 265

  Fly   775 MSGGQATGALGQTPNLGKNKHRRVGSSPDNMHPRHPDRITKTTSGSWEIVEKDGESSPPGTPPPP 839
                                          :.|..|             .:::.....|.|.|..
Zfish   266 ------------------------------LFPESP-------------CQRETPCHSPKTTPRD 287

  Fly   840 YLSSSHMTVLEDPNENNRGAAAAGPGVFIESHQFTPMAGASSPI--PISLHSSHMHAAQSNDTQK 902
            .|:|......||                      ||...:.|.:  |.|...:|:..|:..|..|
Zfish   288 SLNSCPSPDAED----------------------TPDLDSQSSVGSPCSRPVAHIIGAEDEDFGK 330

  Fly   903 EIISMEDENSDLDEPFIDENGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKE 967
            |...|..:.|....  ||          ||:: ....||.|:::|:|..||||||.||..:|||:
Zfish   331 EQEQMNGQCSCFQS--ID----------LLKS-RPAHLAAFIHHVVSQFDPAPLLCYLYADLYKQ 382

  Fly   968 GTSKDMRKWAYEIHSTFLVPRAPLSWYRQDESLAREVDNVLQLEYDKVEILRTVFLRS--RKRAK 1030
            .::|:.|:   .:..||.:.:.........||:..:::             ||....:  |||.:
Zfish   383 SSTKETRR---ILMDTFFIDKTANLKVTVPESIIADLE-------------RTAHSTNAERKRPE 431

  Fly  1031 DL-------------------ISEQLREFQQKRTAGLGTIYGPTDDKLAEAKTDKLREQIIDKYL 1076
            .:                   :.:.|.:|:.||:.|| |:   .:.:||...|:::|:
Zfish   432 QMSEDVHRQYVQQLQDTLLTDVQKNLEDFRHKRSMGL-TL---AEAELARLDTERMRD------- 485

  Fly  1077 MPNLHALIEDENGSPPEDVRKVALCSALSTVIYRIFNTRPPPSSIVERVHHFVSRDKSFKSRIMG 1141
                                :|||                             .:::|....|:.
Zfish   486 --------------------RVAL-----------------------------EKERSCAEHILS 501

  Fly  1142 KNRKMNVRGHPLVLRQYYEVTHCNHCQTIIWGVSPQGYHCTDCKLNIHRQCSKVVDESCPGPLPQ 1206
            |..::      |:..|..|...|:..|.:|              |:..:|....|.|
Zfish   502 KIEEV------LLTSQATEEDKCSTMQFVI--------------LSYMKQLGVKVKE-------- 538

  Fly  1207 AKRLAHNDKI----SKFMGKIRPRTSDVIGNEKRSRQDEELDVELTPDRGQASI--VRQPSDRRP 1265
            .:.|.|..|:    .|....::|....:....|::|..     .:.|.|..:.|  :.||..|.|
Zfish   539 LRGLDHKPKLMNPFHKIKKSMKPEKDGIEEKAKKTRFP-----NVFPQRRPSKIDALSQPKQRTP 598

  Fly  1266 DANISIRSNGNTSCNTSGLNTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSVASTTPSTSGSVA 1330
            ...:.:.:  ....:.|.|.....|||            .|..|.|. |.||...:.|:..|:
Zfish   599 KPQLPLGT--GEQADGSSLPVRSSQSS------------EGSDGTNA-LITSPPYSAPAGQGT-- 646

  Fly  1331 AGLSAFAELNALDTVDKEARRERYSQHPKHKSAPVSVNRSESYKERLSNKRNRNSRRKTSDPSLS 1395
                        |.:.:|.                                      :|.....|
Zfish   647 ------------DAISRET--------------------------------------ETGGLPSS 661

  Fly  1396 SRPNDEQLDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAPGAAGGVQVPPMGLNQNQHPHL 1460
            :.|.       |...|..|....|.|:....:..:..:||
Zfish   662 ALPK-------LGEGTVSGDLQDSSSTNTHFDFSNCMLEH------------------------- 694

  Fly  1461 LIQQHAQQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPLPVARWTLESED----EDDVNEADWS 1521
            |.::.|:....|||         |:.....|..      .|:....::|||    |.::...:|.
Zfish   695 LQEEEAETEKTQDS---------GTPRPDRSRM------DPLGSGEVQSEDDQCVEAELEPPNWQ 744

  Fly  1522 SMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYESGLLSQDHLLLLFPPA 1586
            ::|:.:||:.||..|.||||:|||::.|||.|:|.:|:|:.||:.||....:|         |||
Zfish   745 TLVSRDVLSTLTPQEIKRQEVINELFYTERAHLRMMKVLENLFYQPLIRDAIL---------PPA 800

  Fly  1587 --------LLSLREIH-GAFEQSLKQRRIEHNHVVNTIGDLLADMFDGQSGVVLCEFAAQFCARQ 1642
                    |..:.::| ...||....|:.....::::|||.|...|..:....:......:|:.|
Zfish   801 DIKNIFSNLEEIVQLHLSLTEQMAAVRKKNETALIDSIGDDLLSWFSEEEEDKIKRAVGTYCSNQ 865

  Fly  1643 QIALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLP 1707
            ..|||.:|.|:.||:.....::::||::.||||:|||::|....||||||||.||:.|.|
Zfish   866 PFALELIKSKQKKDQRFNAFIQETESNRHCRRLQLKDIIPVETLRLTKYPLLLENIAKYT----- 925

  Fly  1708 ENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSSYDKE------EFKKLDLT 1766
            |::.|...::||.|..::||..|||.||.||:..:|::.||:||.||..:.      |||.||||
Zfish   926 EDSEERSKVKRAGECCRKILTHVNQEVKEAENKQRLEDYQRRLDLSSLKQSENPMIAEFKSLDLT 990

  Fly  1767 QHHLIHDGNLT--IKKNPSVQLHGLLFENMIVLLTKQDDKYYL----KNLHTPLSITNKPVSPIM 1825
            :..::|:|.|:  :.|:.:::|:.||.|:::|||.:||:|..|    ||| ...:.|....|||:
Zfish   991 KRKMVHEGPLSWKVNKDKTIELYTLLLEDILVLLQRQDEKLILKCHSKNL-AGTAETKHIFSPII 1054

  Fly  1826 SIDADTLIRQEAADKNSFFLIKMKT--SQMLELRAPSSSECKTWFKHFSDVAARQSKNRSKNASS 1888
            .: :..::|..|.|..|||::.|..  :|:.||.|.:.||.|||     .....|..:..|....
Zfish  1055 KL-STVMVRSVATDNRSFFVLSMSDNGAQIYELMAQTVSEQKTW-----QCLITQRADCMKTKLH 1113

  Fly  1889 NHDTSISDPALAAIPHSN-TKESLELSTDTVQPLAA-TATLTTTPL--------APMLPIATVTP 1943
            |         :..:|.:: .:|.:|:....||.|:. |..:|...:        ..::|    ||
Zfish  1114 N---------IIPLPQTDGEREEVEMINSGVQKLSKDTEPITLASIQSPVEKDDTEVIP----TP 1165

  Fly  1944 APATNNSNVSSLTGVQLRNPQRDATASESDADYVNTPKPRSSQNE--VNRTMSIRST-------- 1998
            .|:|              ||..:.:..|::.:..|:.:.::..:|  |...:|.|.|
Zfish  1166 IPST--------------NPFENKSDEEAEEEEENSLQDQAEDDEAFVVADLSDRLTFLKQRSRL 1216

  Fly  1999 GEPIQKYSANGTEAN----DVTLRHSQSTRESVRPGSTGEERNSTYGMVGGNSKRDSASIVCSNN 2059
            |..|.:..|...|..    |..||...:.|:.:....:|:|.......  ...:::...:.|...
Zfish  1217 GIAIDEDEAEVFELQPLIADEALRTLATLRQLLINQFSGQEDTDRENK--SEDQQEHIEMTCPQQ 1279

  Fly  2060 SNNTRTLLMQSPL 2072
            .::. :..:||||
Zfish  1280 GSDD-SAAIQSPL 1291

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ_ARHGEF11-12-like	258..333	CDD:467282	36/74 (49%)
		RGS_GEF_like	934..1055	CDD:188710	34/141 (24%)
		C1_ARHGEF-like	1150..1202	CDD:410382	10/51 (20%)
		RhoGEF	1539..1731	CDD:238091	69/200 (35%)
		PH_16	1756..1876	CDD:436083	45/133 (34%)
arhgef12b	XP_009300160.2	None