Protein Alignment RhoGEF2 and ARHGEF18

DIOPT Version :10

• Sequence 1: NP_477317.1
• Gene: RhoGEF2 / 36915
• FlyBaseID: FBgn0023172
• Length: 2559
• Species: Drosophila melanogaster
• Sequence 2: XP_005272521.1
• Gene: ARHGEF18 / 23370
• HGNCID: 17090
• Length: 1426
• Species: Homo sapiens
• Alignment Length: 1629
• Identity: 321/1629 - (19%)
• Similarity: 530/1629 - (32%)
• Gaps: 563/1629 - (34%)

  Fly  1198 ESCPGPLPQAKRLAHNDKISKFMGKIRPRTSD---VIGNEK---------RSRQDEELDV----- 1245
            :||..|. .:..:|...|.|.  |..:|...|   .:|:::         .|.:|..||:
Human    33 KSCVQPF-DSGAVASATKSSS--GSWKPENPDFFSAMGDDQEDDFPRRLSESMEDLSLDLGALQG 94

  Fly  1246 -ELTPDRGQASIVRQPSDRRPDANISIRSNG--------------------------NTSCNTS- 1282
             |...|.|..:    ||..:|......|..|                          :.||:.|
Human    95 SEYLQDLGLGA----PSHSQPGETPDSRPTGEEPGRDSLFSSLAGSQDLSRRRSWERSRSCSESW 155

  Fly  1283 -----GLNTTDLQSSFHGSCANDSIN-PGGG-----AGCNMDLSTSVAS-----TTPSTSGSVAA 1331
                 ..:..|.:.....:.|:.::| ||||     .||.....|...|     .:|...|...:
Human   156 RRLSLDASAVDEEPCLPRTLASLALNLPGGGLKTWTQGCLSGGGTPAESPGKECDSPKKRGRSRS 220

  Fly  1332 GLSAFAELNA---------------------LDTVDK------------EARRERYSQHPKHKSA 1363
            ...:|.|:.:                     |:.::|            :..:|....|...:.|
Human   221 VPVSFYEIRSPEISPGLEVPTPPVQGLEPPVLECMEKDHVEPDHVLIVQQVLQELRQYHGARQRA 285

  Fly  1364 PVSV----------------------------NRSESYKERLSNKRNRNSRRK--------TSDP 1392
            .:|.                            ::|.|.|.|||..|:|.:|:|        ..|.
Human   286 CMSASPGGAHSNLTWFEFLSESEDGAGKNEKSDKSTSVKRRLSCLRSRVTRQKEKGKSPAHLKDK 350

  Fly  1393 SLSSRP-----NDEQLDLGLSNATYVGSSNSS------LSSAGGSESPSTSMEHFAAPG------ 1440
            ...:|.     |..||..|    |:.|.|:..      ||||...|.|..::....:|.
Human   351 GQDARERRECVNGHQLLQG----TFSGPSSCPLCGKPFLSSASLKEHPRGTLLSDGSPALSRNVG 411

  Fly  1441 ---------------AAGGVQVPPM--GLNQNQHPHLLIQQHAQQYCQQDSFQAGLAGAAGSSAA 1488
                           |..|.|:.|:  .:::.....|..:|.|     .||.         |..:
Human   412 MTVSQKGGPQPTPSPAGPGTQLGPITGEMDEADSAFLKFKQTA-----DDSL---------SLTS 462

  Fly  1489 SNSSFWNAGHPLPVARWTLESEDEDDVNEAD---WSSMVAAEVLAALTDAEKKRQEIINEIYQTE 1550
            .|:.......|...   :|.||.|.|.:|.:   ||..|.|...........|||:::.|:.|||
Human   463 PNTESIFVEDPYTA---SLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYELMQTE 524

  Fly  1551 RNHVRTLKLLDRLFFLPLYESGLLSQDHLLLLFPPALLSLREIHGAFEQSLKQRRIE-------H 1608
            .:||||||::.:::...|.|....|...:..|||.| ..|.|.|..|...||:||.|       .
Human   525 VHHVRTLKIMLKVYSRALQEELQFSSKAIGRLFPCA-DDLLETHSHFLARLKERRQESLEEGSDR 588

  Fly  1609 NHVVNTIGDLLADMFDGQSGVVLCEFAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKACR 1673
            |:|:..|||||...|.|::|..:.|....||:....|:...|....:::..|.|:||..:....|
Human   589 NYVIQKIGDLLVQQFSGENGERMKEKYGVFCSGHNEAVSHYKLLLQQNKKFQNLIKKIGNFSIVR 653

  Fly  1674 RLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAE 1738
            ||.:::.:..|.||:||||:|.|.:.:.|    ...|.:.|.:.:|:...|.|:.:|:..|...|
Human   654 RLGVQECILLVTQRITKYPVLVERIIQNT----EAGTEDYEDLTQALNLIKDIISQVDAKVSECE 714

  Fly  1739 DAHKLQNIQRKLDRSSYDKEE----FKKLDLTQHHLIHDGNLTIKKNPS--VQLHGLLFENMIVL 1797
            ...:|:.|..|:|..|..|.:    |:|.|:.|..|..:|.|..|....  ..:..:|..::::|
Human   715 KGQRLREIAGKMDLKSSSKLKNGLTFRKEDMLQRQLHLEGMLCWKTTSGRLKDILAILLTDVLLL 779

  Fly  1798 LTKQDDKYYLKNLHTPLSITNKPVSPIMSIDADTLIRQEAADKNSFFLI--KMKTSQMLELRAPS 1860
            |.::|.||..      .|:.:||  |::|:. ..::|:.|.::.:.|||  .::..:|.|:...|
Human   780 LQEKDQKYVF------ASVDSKP--PVISLQ-KLIVREVANEEKAMFLISASLQGPEMYEIYTSS 835

  Fly  1861 SSECKTWFKHFSDVAARQSKNRSKNASSNHDTSISDPALAAIPHSNTKESLELSTDTVQPLAATA 1925
            ..:...|..|.         .|:..:..:.:..         |.|..:|..:
Human   836 KEDRNAWMAHI---------QRAVESCPDEEEG---------PFSLPEEERK------------- 869

  Fly  1926 TLTTTPLAPMLPIATVTPAPATNNSNVSSLTGVQLRNPQRDATASESDAD----YV--------- 1977
                           |..|.||.      |...|.|...:|...::|..:    |:
Human   870 ---------------VVEARATR------LRDFQERLSMKDQLIAQSLLEKQQIYLEMAEMGGLE 913

  Fly  1978 NTPKPR-------------------SSQNEVNRTMSI--RSTGEPIQKYSANGTEANDVTLRHSQ 2021
            :.|:||                   |:.:|:....|:  |..|      |||| :|.|
Human   914 DLPQPRGLFRGGDPSETLQGELILKSAMSEIEGIQSLICRQLG------SANG-QAED------- 964

  Fly  2022 STRESVRPGSTGEERNS-TYGMVGGNSKRDSASIVCSNNSNNTRTLLMQSPLVDPTAIQVSISPA 2085
                  ...|||..|.: |:           |...|:|:.....:...:....||       .|.
Human   965 ------GGSSTGPPRRAETF-----------AGYDCTNSPTKNGSFKKKVSSTDP-------RPR 1005

  Fly  2086 HTAEPVLTPGEKLRRLDASIRNDLLEKQKIICDIFRLPVEHYDQIVDIAMMPEAPKDSADIALAA 2150
            ....|..:|..||.  |:.|.....|..:::                     |||...:|
Human  1006 DWRGPPNSPDLKLS--DSDIPGSSEESPQVV---------------------EAPGTESD----- 1042

  Fly  2151 YDQIQTLTKMLNEYMHVTPEQEVSAVSTAVCGHCHEKEKLRKKVAPSSSFSSSPPPLPPPNRQHA 2215
                                                                  |.||
Human  1043 ------------------------------------------------------PRLP------- 1046

  Fly  2216 QAQAQIPPSRLMPKLQT-----LDLDEVAIHEDDDGYCEIDELRLPAIPSKPHERPTTPLAPFNT 2275
                .:..|.|:.::||     |:|..|..|:  |.|.|
Human  1047 ----TVLESELVQRIQTLSQLLLNLQAVIAHQ--DSYVE-------------------------- 1079

  Fly  2276 EPKTSQSVIDASKRQSTDAVPEG--LLEQEPLEGDKTETKGEDNEVKTVPSDKLSESCNEERQCV 2338
               |.::.|...::|.......|  |||||      .:...|....:....:||......|:|..
Human  1080 ---TQRAAIQEREKQFRLQSTRGNLLLEQE------RQRNFEKQREERAALEKLQSQLRHEQQRW 1135

  Fly  2339 EADITKEVADPTTSKNEAAASVDELPSQSREIKTAENASKSVADKK----EDNEETIEEGVASTV 2399
            |    :|..........|.|.:.|...::|:::......::..:::    :.:.|.:.|
Human  1136 E----RERQWQHQELERAGARLQEREGEARQLRERLEQERAELERQRQAYQHDLERLRE------ 1190

  Fly  2400 DSSTQTSPTESPKETDKLTGGSSSTCGPNRIQHASVLEPSVPCHALS-SIVTILNEQISML---- 2459
               .|.:.....:..:.|.........|..:...::.|...|.|..| :...:...::|||
Human  1191 ---AQRAVERERERLELLRRLKKQNTAPGALPPDTLAEAQPPSHPPSFNGEGLEGPRVSMLPSGV 1252

  Fly  2460 ------LPKINERDM--ERERLRKENQHLRELLSALHDRQRVDEVKE------------------ 2498
                  .|::..||.  ...||.|.:..: :||||.:..||...|::
Human  1253 GPEYAERPEVARRDSAPTENRLAKSDVPI-QLLSATNQFQRQAAVQQQIPTKLAASTKGGKDKGG 1316

  Fly  2499 -----------TPFDLKKLMHAEDVEFDDDIDAISNSSLT-----------------PTPTPIPT 2535
                       ..||||:.:....:...|:..:.:..||:                 |.|:|.|.
Human  1317 KSRGSQRWESSASFDLKQQLLLNKLMGKDESTSRNRRSLSPILPGRHSPAPPPDPGFPAPSPPPA 1381

  Fly  2536 ASPS 2539
            .|||
Human  1382 DSPS 1385

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ_ARHGEF11-12-like	258..333	CDD:467282
		RGS_GEF_like	934..1055	CDD:188710
		C1_ARHGEF-like	1150..1202	CDD:410382	2/3 (67%)
		RhoGEF	1539..1731	CDD:238091	65/198 (33%)
		PH_16	1756..1876	CDD:436083	30/127 (24%)
ARHGEF18	XP_005272521.1	C1	361..>390	CDD:412127	11/32 (34%)
		RhoGEF	513..707	CDD:238091	65/198 (33%)
		PH_ARHGEF18	747..865	CDD:275437	29/144 (20%)
		Smc	<1053..>1199	CDD:440809	33/195 (17%)
		PRK13729	1149..>1293	CDD:184281	27/153 (18%)