Protein Alignment RhoGEF2 and Arhgef1

DIOPT Version :10

• Sequence 1: NP_477317.1
• Gene: RhoGEF2 / 36915
• FlyBaseID: FBgn0023172
• Length: 2559
• Species: Drosophila melanogaster
• Sequence 2: XP_006539628.1
• Gene: Arhgef1 / 16801
• MGIID: 1353510
• Length: 980
• Species: Mus musculus
• Alignment Length: 1276
• Identity: 305/1276 - (23%)
• Similarity: 490/1276 - (38%)
• Gaps: 393/1276 - (30%)

  Fly   858 GAAAAGPGVFIESHQFTPMAGASSPIPISLHSSHMHAAQSNDTQKEIISMEDEN--SDLDEPFID 920
            |.||.||          |.:|.     :|:                ||..|||:  ::|:....|
Mouse     6 GGAAPGP----------PRSGL-----VSI----------------IIGAEDEDFENELEANSED 39

  Fly   921 ENGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKDMRKWAYEIHSTFL 985
            :|..|.:|.::  ......|...|.:|....:|.|||..|..::......|:.:|...:.:.:||
Mouse    40 QNSQFQSLEQV--KRRPAHLMALLQHVALQFEPGPLLCCLHADMLSSLGPKEAKKAFLDFYHSFL 102

  Fly   986 ----VPRAPLSWYRQDESLAREVDNV---LQLEYDKVEILRTVFLRSRKRAKDLISEQLREFQQK 1043
                |.|.|:     ..|:|.|:|..   |..|..:...::.| ::|::.|   :|.||.:|:.|
Mouse   103 EKTAVLRVPV-----PPSVAFELDRTRPDLISEDVQRRFIQEV-VQSQQAA---VSRQLEDFRSK 158

  Fly  1044 RTAGLGTIYGPTDDKLA-------------EAKTDKLREQIIDKYLMPNLHALIEDENGSPPEDV 1095
            |..|:    .|.:.:|:             ||:...:.|::: .:|....|.:..||.
Mouse   159 RLMGM----TPWEQELSLLEPWIGKDRGNYEARERHVAERLL-SHLEETQHTISTDEE------- 211

  Fly  1096 RKVALCSALSTVIYRIFNTRPPPSSIVERVHHFVSRDKS---------FKSRIMGKNR------- 1144
            :..|:.:|:|  :|               :.|...|.||         |:.::||..|
Mouse   212 KSAAVVTAIS--LY---------------MRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPPKT 259

  Fly  1145 -----------KMNVRGHPLVLRQYYEVTHCNHCQTIIWGVSPQGYHCTDCKLNIHRQCSKVVDE 1198
                       :.| ||.|       ....|.|                   |.:.....|
Mouse   260 KKGLSSILDPARWN-RGEP-------SAPDCRH-------------------LKVEADAEK---- 293

  Fly  1199 SCPGPLPQAKRLAHNDKISKFMGKIRPRTSDVIGNEKRSRQDEELDVELTPDRGQASIVRQPSDR 1263
              |||..:...|..:.         |.||   :|               ||.:....:...|
Mouse   294 --PGPADRKGGLGMSS---------RDRT---VG---------------TPGQDNPGVSLHP--- 326

  Fly  1264 RPDANISIRSNGNTSCNTSGLNTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSVASTTPSTSGS 1328
                                |:|..:          ||..||      :|.......|.|.
Mouse   327 --------------------LSTDSV----------DSREPG------VDTPQEPGDTPPQ---- 351

  Fly  1329 VAAGLSAFAELNALDTVDKEARRERYSQHPKHKSAPVSVNRSESYKERLSNKRNRNSRRKTSDPS 1393
               |.::...|...::.:.....||     :.|.....:.||||.  |:|:      ||:.|..|
Mouse   352 ---GPTSLEPLAPPESTEDNGETER-----RWKRLSGRLGRSESL--RVSD------RRRPSRGS 400

  Fly  1394 LSSRPNDEQLDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAPGAAGGVQVPPMGLNQNQHP 1458
            |.::                       ...||.......|:    ||:|..|    :|..:...|
Mouse   401 LGAK-----------------------GRGGGRSRSDVDMD----PGSATAV----LGPTRRATP 434

  Fly  1459 HLLIQQHAQQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPLPVARWTLESEDEDDVNEADWSSM 1523
                             :.|..|..|.|.                   ||.|.|:   ...|..:
Mouse   435 -----------------EPGDDGEPGRSG-------------------LELEPEE---PPGWREL 460

  Fly  1524 VAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYESGLLSQDHLLLLFPPALL 1588
            |..:.|.:|..::.||||:|:|:..||..|||.|::|..||:.|:.:.|....|.|..:| |:|.
Mouse   461 VPPDTLLSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMADGGFFPLDELQNIF-PSLD 524

  Fly  1589 SLREIHGAFEQSLKQRRIEHNHVVNTIGDLLADMFDGQSGVVLCEFAAQFCARQQIALEALKEKR 1653
            .|.|:|..|...|.:||.|..:::..|||:|...|||..|....:.:::||:||..|||.||.|:
Mouse   525 ELIEVHSLFLDRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQ 589

  Fly  1654 NKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPENTTEAEAIQR 1718
            .|:......::::||...||||:|||::||.:|||||||||.:::.:.|     |.:||...::.
Mouse   590 RKEPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNT-----EESTERGKVEL 649

  Fly  1719 AVESSKRILVEVNQAVKTAEDAHKLQNIQRKLD----RSSYDK--EEFKKLDLTQHHLIHDGNLT 1777
            |.|..:.||..|||||:..||..:|::.||:||    |.|.|.  .|||.||:|:..|:|:|.||
Mouse   650 AAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLTHLRQSSDPMLSEFKNLDITKKKLVHEGPLT 714

  Fly  1778 --IKKNPSVQLHGLLFENMIVLLTKQDDKYYLKNLH----TPLSITNKPVSPIMSIDADTLIRQE 1836
              :.|:.::::|.||.:::::||.:||::..||: |    ||.......:.|::.: ...:.|:.
Mouse   715 WRVTKDKAIEVHVLLLDDLLLLLQRQDERLLLKS-HSRTLTPTPDGKTMLRPVLRL-TSAMTREV 777

  Fly  1837 AADKNSFFLIKM--KTSQMLELRAPSSSECKTWFKHFSDVA------ARQSKNRSKNASSNHDTS 1893
            |.|..:|::|..  :.:|:.||.|.:|||.|.|....::.|      |..|:.:.:.:.|    |
Mouse   778 ATDHKAFYVIFTWDQEAQIYELVAQTSSERKNWCNLITETAGSLKVPAPASRLKPRPSPS----S 838

  Fly  1894 ISDPALAAIPHSNTKESLELSTDTVQPLAATATLTTTPLAPMLPIATVTPAP-----ATNNSNVS 1953
            |.:|.|:         |.|..|...:...|.|. |...|..:||..  .|.|     ||....|.
Mouse   839 IREPLLS---------SSENGTGGAEMAPADAR-TERLLNDLLPFC--RPGPEGQLAATALQKVL 891

  Fly  1954 SLTGVQLRNPQRDATASESDADYV---NTPKPRSSQNEVNRTMSIRSTGEPIQKYSANGTEANDV 2015
            ||..:.|...:........|.|.|   ..|.|..:|......:|:                  :|
Mouse   892 SLKQILLSTEEDSGAGPPRDGDGVPGGRAPGPVHTQEIEENLLSL------------------EV 938

  Fly  2016 TLRHSQSTRE--------------SVRPGSTGEERNSTYGM 2042
            .:|..:...|              ::.|.....||::..|:
Mouse   939 AIRQLEELEEEFCRLRPLLSQLGGTLSPNLAAPERSAQTGL 979

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ_ARHGEF11-12-like	258..333	CDD:467282
		RGS_GEF_like	934..1055	CDD:188710	31/127 (24%)
		C1_ARHGEF-like	1150..1202	CDD:410382	6/51 (12%)
		RhoGEF	1539..1731	CDD:238091	76/191 (40%)
		PH_16	1756..1876	CDD:436083	41/135 (30%)
Arhgef1	XP_006539628.1	RGS_p115RhoGEF	50..242	CDD:188709	48/231 (21%)
		PHA03247	<277..462	CDD:223021	59/372 (16%)
		RhoGEF	479..663	CDD:214619	74/189 (39%)
		PH_p115RhoGEF	697..821	CDD:275429	39/125 (31%)