Protein Alignment RhoGEF2 and arhgef18

DIOPT Version :10

• Sequence 1: NP_477317.1
• Gene: RhoGEF2 / 36915
• FlyBaseID: FBgn0023172
• Length: 2559
• Species: Drosophila melanogaster
• Sequence 2: XP_031760977.1
• Gene: arhgef18 / 100498531
• XenbaseID: XB-GENE-990867
• Length: 1532
• Species: Xenopus tropicalis
• Alignment Length: 1835
• Identity: 347/1835 - (18%)
• Similarity: 592/1835 - (32%)
• Gaps: 640/1835 - (34%)

  Fly   787 TPNLGKNKHRR--VGSSPDNMHPRHPDRITKTTSGSWEIVEKDGESSPPGTPPPPYLSSSHMTVL 849
            ||:...|....  :....|::.....|......|.|.::...|  ||..||  ..|.....|...
 Frog    73 TPDADPNSEEAFLLAEEKDDLSVDLNDYSNDIISSSEDLTSID--SSLQGT--EYYKDLVQMDAA 133

  Fly   850 EDP---NENNRGAAAA------------------------------GPGVFIESH---------- 871
            |||   .|.::.|:|:                              |||..||.:
 Frog   134 EDPRSEQEEHKLASASFCMADETDITLGKGEVIITYTKPFICPVSCGPGAAIEENITENTTEGDK 198

  Fly   872 ----QFTPMAGASSPIPI---SLHSSHMH----AAQSNDTQKEIISMEDENSDLDEPFIDEN--- 922
                |..|:. ...|||:   ||.:|..|    |...:||.:.:.....|.::..|..|:|:
 Frog   199 GSYRQLLPIK-FEEPIPVLVRSLSTSRRHSWDDAVSPSDTVRRLSLSNSEIANDGERDIEESAAA 262

  Fly   923 ---------GPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKDMRKWAY 978
                     ||:           ||.|:|       .:|...|.........||..|...|..:.
 Frog   263 TMVHSMELPGPW-----------VTNLSI-------QADVGELGVQETNAESKEVDSHGKRLRSK 309

  Fly   979 EIHSTFLVPRAPLSWYRQDESLAREVDNVLQLEYDKVEILRTVFLRSRKRAKD--------LISE 1035
            .:.||.            |:.....:...|:.....:|:::|..|.:.:  ||        .:.:
 Frog   310 SVPSTL------------DKISTTRISRSLESSCPVIEVIQTPHLETTE--KDHVEPTHVLFVQQ 360

  Fly  1036 QLREFQQKRTAGLGTIYGPTDDKLAEAKTDKLREQIIDKYLMPNLHALIEDENGSPPEDVRKVAL 1100
            .|:|.:|         |..|.:|...::..|  :.:.....:.|     |.|..:..|.|.|
 Frog   361 VLQELKQ---------YHGTKNKQEGSREPK--QNLTWFEFLSN-----EPEETTKSEKVEK--- 406

  Fly  1101 CSALSTVIYRIFNTRPPPSSIVERVHHFVSRDK-SFKSRIMGKNRK-----MNVRGHPLVLRQYY 1159
                .|.:.|..      ||:..||.....:|| ..|..:..|.::     :|..||.||...:.
 Frog   407 ----GTKVKRRL------SSLRSRVTGSWQKDKGKIKEELKDKGKEAKDTWINTNGHELVPGCFS 461

  Fly  1160 EVTHCNHCQTIIWGVSPQGYHCTDCKLNIHRQCSKVVDESCPGPLPQAKRLAHNDKISKFMGKIR 1224
            ....|..|...:  .:..|..|..|.:|:|:.|..::.| |.           ::|:.|
 Frog   462 SHAKCTLCTKAL--ANRHGLQCMYCAVNVHKNCKALLPE-CT-----------SNKLKK------ 506

  Fly  1225 PRTSDVIGNEKRSRQDEELDVELTPDRGQASIVRQPSDRRPDANISIRSNGNTSCNTSGLNTTDL 1289
                                 :.:|....:|:..|.:..:.:..|:|.....|.....||..|
 Frog   507 ---------------------DNSPKPVASSLSCQQASLKENPRIAIVGLDGTHAQARGLGMT-- 548

  Fly  1290 QSSFHGSCANDSINPGGGAGCNMDLSTSVASTTPSTSGSVAAGLSAFAELNALDTVDKEARRERY 1354
                        :...||:...:.          |.|||.|:..:|     .:|.||....|.:.
 Frog   549 ------------VTHRGGSRVEIQ----------SNSGSRASTSTA-----EMDDVDSGLSRLKL 586

  Fly  1355 SQHPKHKSAPVSVNRSESYKERLSNKRNRNSRRKTSDPSLSSRPNDEQLDLGLSNATYVGSSNSS 1419
            ........||.||...:||    :|.::                   :|||
 Frog   587 FSDENVSLAPDSVFLEDSY----TNVKS-------------------ELDL-------------- 614

  Fly  1420 LSSAGGSESPSTSMEHFAAPGAAGGVQVPPMGLNQNQHPHLLIQQHAQQYCQQDSFQAGLAGAAG 1484
                       .::|                                        |:||
 Frog   615 -----------DALE----------------------------------------FEAG------ 622

  Fly  1485 SSAASNSSFWNAGHPLPVARWTLESEDEDDVNEADWSSMVAAEVLAALTDAEKKRQEIINEIYQT 1549
                                              .||..|....|........|||::|.|:.||
 Frog   623 ----------------------------------SWSCAVDPLYLKKQKKEVIKRQDVIYELMQT 653

  Fly  1550 ERNHVRTLKLLDRLFFLPLYESGLLSQDHLLLLFPPALLSLREIHGAFEQSLKQRRIE------- 1607
            |.:||||||::.|::...|.|........:..:| |.:..|.|:|..|....|:||.|
 Frog   654 EMHHVRTLKIMLRVYSRALSEELQYGNKDIHQIF-PCVDDLLELHVNFLARFKERRKESMEEGSD 717

  Fly  1608 HNHVVNTIGDLLADMFDGQSGVVLCEFAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKAC 1672
            .|:::..:||:|...|.|:||..:.|....||::...|:...|:.....:..|.|:||..:....
 Frog   718 RNYMIQKVGDILVQQFSGESGERMREKYGIFCSQHNKAVSRYKDLLRDSKKFQNLMKKIGNSSIV 782

  Fly  1673 RRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTA 1737
            |||.:::.:..|.||:||||:|.|.:.:.|    .|.|.:.|.:.:|:...|..:.:|:..|..:
 Frog   783 RRLGVQECILLVTQRITKYPVLVERIIQNT----EEGTEDYETLTQAIALIKDTITDVDSRVHES 843

  Fly  1738 EDAHKLQNIQRKLDRSSYDKEE---FKKLDLTQHHLIHDGNLTIKKNPSVQLH---GLLFENMIV 1796
            |...:|:.|..|::..|..|.:   |:|.|:.:..|:|||.| ..|..|.:|.   .:|..::::
 Frog   844 EKVQRLREIVSKMELKSSGKLKNGTFRKQDILRRQLLHDGVL-YWKTASGRLKDILAVLLTDILL 907

  Fly  1797 LLTKQDDKYYLKNLHTPLSITNKPVSPIMSIDADTLIRQEAADKNSFFLIKMKT--SQMLELRAP 1859
            ||.::|.||.|.      .:.||  :|::|:. ..::|:.|.::.:.|||....  ::|.|:...
 Frog   908 LLQEKDQKYSLS------PVDNK--APVISLQ-KLIVREVANEEKAMFLISASNTGAEMYEIHTN 963

  Fly  1860 SSSECKTWF-------------------------KHFSDVAAR----------------QSKNRS 1883
            |..|...|.                         |.|::.||:                ||.|..
 Frog   964 SKEERNFWMNIIRGAVESCPVQDAEILNEAEEEKKLFTERAAKIKEFQERLNQKDYVIVQSLNEK 1028

  Fly  1884 KNASS------NHDTSISDPALAAIPHSNTKESLELSTDTVQPLAATATLTTTPLAPML--PIAT 1940
            .....      .::....:|.:..:|.:.|.|:||     .:.:..:|......:..||  ..|.
 Frog  1029 LQTCMEMAELYGYEDLPQEPRITLLPRAETGENLE-----GKGILHSAVTAAERIHYMLLSQTAL 1088

  Fly  1941 VTPAPATNNSNVSSLTGVQLRNPQRDATASESDADYVNTPKPRSSQNEVNRTMSIRSTGEPIQKY 2005
            :.|....|||....        |:|..|....|.|
 Frog  1089 LNPLDEANNSATIL--------PRRSETFGGYDTD------------------------------ 1115

  Fly  2006 SANGTEANDVTLRHSQSTRESVRPGSTGEERNSTYGMVGGNSKRDSASIVCSNNSNNTRTLLMQS 2070
                      :.:.....|.:|..||..:.::.|.           ...|.|...||:..:..::
 Frog  1116 ----------SFKQKVKDRRAVSMGSDSQVQDGTL-----------IEPVVSTTDNNSPPVCHET 1159

  Fly  2071 PLVDPTAIQVSISPAHTAEPVLT----------PGEKLRRLDASIRNDLLEKQK----------- 2114
            .::  |.::..:...::.:.|::          ..||..|..:|..|.|||::|
 Frog  1160 EMI--TCVETLLQHLYSLQAVISQQDSYVEVQKEREKQFRQQSSRGNMLLEQEKQRNFEKQREEL 1222

  Fly  2115 ----IICDIFRLPVEHYDQIVDIA---------MMPEAPKDSADIALAAYDQIQTLTKMLNEYMH 2166
                .:.|..||..:.:::..:..         |:.:...||.......:.:...|.....||.|
 Frog  1223 GNVQKMQDQLRLEKQKWERERERQQREAEAMENMLRQRMGDSELQLQKLHQERDELEAQRKEYQH 1287

  Fly  2167 VTPEQEVSAVSTAVCGHCHEKEKLRKKV----APSSSFSSSPPPLPPPNRQHAQAQAQIPPSRLM 2227
               :.|....|........|:.:|.|::    |.:.||||
 Frog  1288 ---DLERLRESQKAVEKDRERLELMKRIKATSAGAGSFSS------------------------- 1324

  Fly  2228 PKLQTLDLDEVAIH---EDDDGYCEIDELRLPAIPSKPHERPTTPLAPFNTEPKTSQSVI----- 2284
                  ||..|.:|   .:.:|....:         .|...|..|||      ||:.|:.
 Frog  1325 ------DLIPVVVHSAASNGEGMLSTE---------APSHLPLKPLA------KTTMSMSAADYL 1368

  Fly  2285 ---DASKRQS--TDAVPEGLLEQEPLEGDKTETKGEDNEVKTVPSDKLSESCNEERQCVEADITK 2344
               :.::|:|  ||..|  .|::|                  ||...||.:...::|
 Frog  1369 ERPEVARRESNVTDIRP--ALKKE------------------VPIHLLSATNQIQKQ-------- 1405

  Fly  2345 EVADPTTSKNEAAASVDELPSQSREIKTAENASKSVADKKEDNEETIEEGVA----------STV 2399
                       ||.:..::|:.....|..|..|:..|..:.::..:::..:.          .||
 Frog  1406 -----------AALTRQQIPTHLAMDKGKEKPSRGKASHRTESTASLDHRLGFPPKLSGKEDGTV 1459

  Fly  2400 DSSTQTSPTESPKET 2414
            .|....||:....:|
 Frog  1460 RSRRSASPSPFSSQT 1474

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ_ARHGEF11-12-like	258..333	CDD:467282
		RGS_GEF_like	934..1055	CDD:188710	23/128 (18%)
		C1_ARHGEF-like	1150..1202	CDD:410382	14/51 (27%)
		RhoGEF	1539..1731	CDD:238091	61/198 (31%)
		PH_16	1756..1876	CDD:436083	35/152 (23%)
arhgef18	XP_031760977.1	C1_ARHGEF18-like	450..502	CDD:410429	14/65 (22%)
		RhoGEF	643..837	CDD:238091	61/198 (31%)
		PH_ARHGEF18	876..994	CDD:275437	29/127 (23%)
		DUF5401	<1179..>1314	CDD:375164	24/137 (18%)