Protein Alignment RhoGEF2 and arhgef1

DIOPT Version :10

• Sequence 1: NP_477317.1
• Gene: RhoGEF2 / 36915
• FlyBaseID: FBgn0023172
• Length: 2559
• Species: Drosophila melanogaster
• Sequence 2: XP_012822895.3
• Gene: arhgef1 / 100037905
• XenbaseID: XB-GENE-1009790
• Length: 1128
• Species: Xenopus tropicalis
• Alignment Length: 1299
• Identity: 322/1299 - (24%)
• Similarity: 533/1299 - (41%)
• Gaps: 333/1299 - (25%)

  Fly   825 EKDGESSPPGTPPPPYLSSSHMTVLEDPNENNRGAAAAGPGVFIESH-----QFTPMAGASSPIP 884
            :|.|....||           :.:.....:.:||        |..||     |.||     .|:.
 Frog    18 QKHGSQVSPG-----------LCITYASADLSRG--------FPLSHSLGFCQTTP-----KPLV 58

  Fly   885 ISLHS-----SHMHAAQSNDTQKEIISMEDEN--SDLDEPFIDENGPFNNLTRLLEAENVTFLAI 942
            ..:.|     .|.....|..:. .||..|||:  :|::...:||:..|.::.  |..|:..::.:
 Frog    59 CQIRSMDPDDGHFGGLYSKGSM-SIIGAEDEDFENDIEPNMVDESSYFRSIE--LVKEHPAYIMV 120

  Fly   943 FLNYVISNSDPAPLLFYLITELYKEGTSKDMRKWAYEIHSTFLVPRAPLSWYRQDESLAREVDNV 1007
            ||.:|:...||:|||.:|..|:.|..::|:::|...:.|.|||:..|.|.         ..|.|.
 Frog   121 FLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLH---------VSVPNS 176

  Fly  1008 LQLEYDKVEILRTVFLRSRKRAKDL-------ISEQLREFQQKRTAGL--GTI-------YGPTD 1056
            :..|.|:...........||..||:       ||:||.:|:.||..|:  |.:       |...|
 Frog   177 VSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTND 241

  Fly  1057 DKLAEAKTDKLREQIIDKYLMPNLHALIEDENGSPPEDVRKVALCSALSTVIYRIFNTRPPPSSI 1121
            ..:.|||..:|.||::.:  :..:|..|     :|.|:......|:.::.:.:....|:...|
 Frog   242 RSILEAKEKQLAEQLLAR--LDEMHLTI-----APDEEKSSAIFCAIVAYMKHLGVKTKAADS-- 297

  Fly  1122 VERVHHFVSRDKSFKSRIMGKNRKMNVRGHPLVLRQYYEVTHCNHCQTIIWGVSPQGYHCTDCKL 1186
                       |..|.....|....|.:..||..::.:.:            :.|..::..|
 Frog   298 -----------KKSKGIFFRKKVSTNKKDEPLKTKKTFSI------------LDPMRWNRND--- 336

  Fly  1187 NIHRQCSKVVDESCPGPLPQAKRLAHNDKISKFMGKIRPRTSDVIGNEKRSRQDEELDVELTPDR 1251
                  |..||.......|:|:             |..|        ::|..|.:.     |.||
 Frog   337 ------SHYVDSRPSKVEPEAE-------------KPNP--------DRRVSQGQR-----TFDR 369

  Fly  1252 GQASIVRQPSDRRPDANISIRSNGNTSCNTSGLNTTDLQSSFHGSCANDSINPGGGAGCNMDLST 1316
            ..:|..:..:...||:.:|:    |.....:..|..| .:...|:.:||              ::
 Frog   370 SDSSTAKSKNSEGPDSVVSV----NIIPPPNEPNDPD-PAVTDGALSND--------------AS 415

  Fly  1317 SVASTTPSTSGSVAAGLSAFAELNALDTVDKEARRERYSQHPKHKSAPVSVNRSESYK------- 1374
            .|...:|:|....|            |..::.|..||     :.|.....:.||||.:
 Frog   416 EVLKGSPTTEHCPA------------DQTEESAENER-----RKKRLSGKLGRSESLRVYERKRS 463

  Fly  1375 ERLSNKRNRNSRRKTSDPSLSSRPNDEQLDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAP 1439
            :|.|:|..:...|...|...::|.||.|            .:...|....|:.:|.
 Frog   464 QRGSSKAKQTRSRSDVDLEAAARANDLQ------------GTTGKLGPKPGNYTPE--------- 507

  Fly  1440 GAAGGVQVPPMGLNQNQHPHLLIQQHAQQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPLPVAR 1504
                     |.|.::...|.||.|
 Frog   508 ---------PGGSSEGPLPFLLPQ----------------------------------------- 522

  Fly  1505 WTLESEDEDDVNEAD-----WSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLF 1564
               ..|.|..|.||:     |..:|..|:|..|..:|.||||:|||::.||..|||.|::|..:|
 Frog   523 ---SEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVF 584

  Fly  1565 FLPLYESGLLSQDHLLLLFPPALLSLREIHGAFEQSLKQRRIEHNHVVNTIGDLLADMFDGQSGV 1629
            ::|: ::|.:....|..:| |:|..|.|.|..|..:||:.|:|:|.|:..||.:|.:.|||..|.
 Frog   585 YIPM-QNGWIPTLELENIF-PSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGN 647

  Fly  1630 VLCEFAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLL 1694
            ...:.:::||:||..|||.||.|:.:|....:.::::||...||||:|||::|..:|||||||||
 Frog   648 WFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLL 712

  Fly  1695 FENLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSSYDK-- 1757
            .:|:..:|     |...|.:.:|||.||.::||..|||.|:..|:..||.:.||:||.|:..:
 Frog   713 LQNIANLT-----EEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLTDYQRRLDVSNLRQIN 772

  Fly  1758 ----EEFKKLDLTQHHLIHDGNLT--IKKNPSVQLHGLLFENMIVLLTKQDDKYYLK---NLHTP 1813
                .|||.:|:::.:|||:|:||  :.::.:..:|.||.:::::||.|||::..||   ....|
 Frog   773 DPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIP 837

  Fly  1814 LSITNKPVSPIMSIDADTLIRQEAADKNSFFLI--KMKTSQMLELRAPSSSECKTWFKHFSDVAA 1876
            .......:|||:.::. .::|:.|.|||:||:|  ....:|..||.||:.:|.|.||...|:...
 Frog   838 APDGKLMLSPIIKLNM-AMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTG 901

  Fly  1877 -----RQSKNRSKN------------------ASSNHDTSISDPALAAIPHSNTKESLELSTDTV 1918
                 ..||.|.::                  :||.:..|:.|    ::.....:||:|.:.:.
 Frog   902 LLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKD----SVSKDEKEESVEDALEG- 961

  Fly  1919 QPLAATATLTTTPLAPMLPIATVTPAPATNNSNVSS--------LTGVQLRNPQRDATASESDAD 1975
            |.....:.||...||..:.:   ....|:...||:|        |..:.:||.:...     |||
 Frog   962 QEEKDFSGLTEYLLASNIDL---NQDAASGGGNVASEALEEVSVLKRLLVRNIRLSV-----DAD 1018

  Fly  1976 YVNTPKPRSSQNEVNRTMSIRSTG-EPIQKYSANGTEANDVTLRHSQSTRESVRPGSTGEERNS 2038
            .::             |:...|.| ||..:.|..|..:|. ......|..||.:.|..|.:::|
 Frog  1019 TLS-------------TLETWSAGEEPALEASEQGRASNG-DREAEDSEMESTQQGENGRKQSS 1068

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ_ARHGEF11-12-like	258..333	CDD:467282
		RGS_GEF_like	934..1055	CDD:188710	38/136 (28%)
		C1_ARHGEF-like	1150..1202	CDD:410382	7/51 (14%)
		RhoGEF	1539..1731	CDD:238091	78/191 (41%)
		PH_16	1756..1876	CDD:436083	42/132 (32%)
arhgef1	XP_012822895.3	RGS_p115RhoGEF	111..303	CDD:188709	54/220 (25%)
		RhoGEF	562..745	CDD:214619	76/189 (40%)
		PH_p115RhoGEF	779..903	CDD:275429	41/124 (33%)