Protein Alignment shot and Ppl

DIOPT Version :10

• Sequence 1: NP_725339.1
• Gene: shot / 36542
• FlyBaseID: FBgn0013733
• Length: 8805
• Species: Drosophila melanogaster
• Sequence 2: NP_001100446.1
• Gene: Ppl / 302934
• RGDID: 1305511
• Length: 1754
• Species: Rattus norvegicus
• Alignment Length: 2336
• Identity: 491/2336 - (21%)
• Similarity: 859/2336 - (36%)
• Gaps: 740/2336 - (31%)

  Fly   436 RMNTALQDREVILQQEIERLERLQRLADKVQREIKHVDQKL-TDL----EGRIGEEGRRIERLHP 495
            :.:..:|.|.:..::..:.:|:||:.||:|:|.|...:.|: :||    :|::.|  .|...|..
  Rat    12 KYSPTVQTRSISNKELSDLIEQLQKNADQVERNIVDTEAKMQSDLARMQDGQLPE--HRDATLQN 74

  Fly   496 V-DAKSIVEALETE---IRHLEEPIQDM-NQDCHVLNEGRYPHVSELHKKVNKLHQRWAQLRTNF 555
            | |::.::..||.:   .:|::.|..|| .:|...|.| |..::...||::..|..|.|..:.|:
  Rat    75 VSDSEKLLYVLEADSAIAKHMKHPQGDMIAEDIRQLKE-RVTNLRGKHKQMYSLAVREADPKVNW 138

  Fly   556 HTNLVQKLSGLKYPVHETTVTRQTRMVVESRQIDTNPHFRDLQEHIEWCQNKLKQLLAADYGSDL 620
            .. ||.:                                            ||.:|.:..:|:||
  Rat   139 DA-LVDE--------------------------------------------KLDKLSSQSFGTDL 158

  Fly   621 PSVKEELDRQQHEHKIIDQFHTKILNDERQQTKFSGDELALYQQRLNQLQKVYAELLSTSTKRLS 685
            |.|    |.|..:|.|   ||.::        |..|..||..:::.::||..|.:||:.|..|..
  Rat   159 PLV----DSQVEQHNI---FHNEV--------KAIGPHLAKDKEQNSELQAKYQKLLAASQARQQ 208

  Fly   686 DLDSLQHFLGQASAELQWLNEKEQVEITRDWADKQLDLPSVHRYYENLMS-ELEKREMHFATILD 749
            .|.|||.::.:.:.||.||:::.:..|..||:|:.||.||..|.|||.:: .||.:|.....:..
  Rat   209 HLSSLQDYMQRCTNELYWLDQQAKGRIQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHT 273

  Fly   750 RGEALLNQQHPASKCIEAHLTALQQQWAWLLQLTLCLEVHLKHATEYHQFFGEIKDAEQWLAKRD 814
            .|:.||..:||....||||:.|:..:|...|.|.:|.|.|||:..:||||..::|||::.|.|.|
  Rat   274 EGDQLLTAEHPGRNSIEAHMEAVHAEWKEYLNLLICEESHLKYMEDYHQFHKDMKDAQELLRKVD 338

  Fly   815 EILNSKFSQSDFGLDQGETLLRGMQDLREELNAFGETVATLQRRAQTVVPLNKRRQPVNRQGPVQ 879
            ..||.|:|.......|.|.|||.:.|..:.|:.:.:.|..||:|.|.|:||..||:...:..||:
  Rat   339 SDLNQKYSPDFKDRYQIELLLRELDDQEKALDKYEDVVRGLQKRGQQVLPLKYRRETPLKPIPVE 403

  Fly   880 AICAYK-QQGQLQIEKGETVTLLDNSGRVKWRVRTAKGQEGPIPGACLLLPPPDQEAIDAAERLK 943
            |:|.:: .||  .|.:|.:.||..|:|. .|.:..:.|::...|..|.::||.|.||:..::.|.
  Rat   404 ALCDFEGDQG--LISRGYSYTLQKNNGE-SWELTDSTGKKLMAPAVCFIIPPTDPEALALSDSLG 465

  Fly   944 RLFDRSVALWQK----KHLRLRQNMIFATIRVVKGWDFDQFLAMGPEQRTAIRRALNDDADKLLS 1004
            ..: |||.  ||    ||                          ..:||..:.|..|
  Rat   466 SQY-RSVR--QKAAGSKH--------------------------ALQQRHEVLRTEN-------- 493

  Fly  1005 EGDPNDPQLRRLRREMDEVNRLFDEFEK-----------RARAEEESKQASRIFTEECLAIKSKL 1058
            .||.:|.|.|:|...:|:|....|..||           :.||.|:|.:.::           .|
  Rat   494 PGDASDLQGRQLLAGLDQVASDLDRQEKAITGILRPPLEQGRAIEDSAERAK-----------HL 547

  Fly  1059 EDMARELDQIILAPLPRDLDSLEHVLEIHSDYERRLHLLEPELKHLQETFRTIAL--------KT 1115
            :::..||.||                             |||  .:|.|....|.        .|
  Rat   548 KNITNELLQI-----------------------------EPE--KIQRTAECEAFVQALPGSGTT 581

  Fly  1116 PVLKKSLDNLMELWKELNTQSGLHKDRLKLLEASLAGLEDNEHVISELENELARHQDLPSTAEGL 1180
            |:||..:::..:.:|.|          ::||||:    ::...|.:.|||.|.|.::|.::.|.
  Rat   582 PLLKTRVEDTNQKYKRL----------VQLLEAA----QEKVDVANRLENSLQRGRELLASHEN- 631

  Fly  1181 QQVFKQLNHMQDIITQQQPQMDKMNDAADQLGRMGVPTKVLGDLKRLHSNVERLNTRWSAVCNQL 1245
                   ..|||   ...|:.|.|.|:..|                   .:|.:.:...|..:.|
  Rat   632 -------RLMQD---DTIPESDYMLDSKRQ-------------------ELEAMASELQAQKSLL 667

  Fly  1246 GERMRSCETAIGLMKNLQSSVQVEESWVDGTTERLSAMPTATSAYELDKL------LGAAIERKP 1304
            ||..::.:.|.....:|.|..|  |...|  .||..|        |:.||      |...:||
  Rat   668 GEVEQNLQVAKQCSSSLASRFQ--EHCPD--LERQEA--------EVHKLNQRFNNLNQQVER-- 718

  Fly  1305 KIENVNVAGGRLIREAKIYDSKCLRF---VDWLVEARPSFSPPRRDLRPADSDPGATQYYSQRLD 1366
            :.:::..|     |:|  |...|..:   :.:||:. ||:.|...|                .|.
  Rat   719 RAQSLQSA-----RDA--YSEYCRGYDHVLQFLVKT-PSYEPQETD----------------SLS 759

  Fly  1367 NLNTK---NDRLLEQLSQRLKTAIEVNGSDGLQYAESLQKPLKTFRVDFSAGSVPTGDGYAARQE 1428
            .:.||   ...||::::...:.|.:|. :|..||.::::                   .|....|
  Rat   760 QMETKLKNQKNLLDEIASMEQGAQKVY-ADSQQYQQAVK-------------------DYELEAE 804

  Fly  1429 DLYTTTYSTTQISSTKTTKSSTKSVYSSDGLDAASQEVSTAGLPQSQIQFNEIRTLKRSQQLGGH 1493
            .|    .|...:.:.:.:..:.::...|.......:|.:.|.      :|.|:..:.|.
  Rat   805 KL----RSLLDLENGRNSHVNKRARLQSPAAKVKEEEAALAA------KFTEVNAINRQ------ 853

  Fly  1494 SVLDIAGIRDPRTGRVLTIGEAIQLRILDVRTGEMLVGDRRITLEQAADQGLIDLQLAKQLLEPG 1558
                          |:..:..|:.|                  |.|..:.|:....|  |..|.|
  Rat   854 --------------RLQNLEFALNL------------------LRQQPEAGVTHETL--QGREQG 884

  Fly  1559 AGRDASGRELSLLEVIQREISEAESGYETAEKRIKVNNTVTVEQTSGELGSPENPRNIADAITAG 1623
            :|.:.:.:       ||:|:.      |..|:|.::.|.|...|        |..:.:.|.....
  Rat   885 SGMEETWK-------IQKELE------EEMERRQQLENEVKSAQ--------EEIQTLKDQGPQE 928

  Fly  1624 SVDTKTGLYRV------KSGQTI--SLAEAYERGYLIRHESVTIKSNALCLSDAIAHGLVDGAGW 1680
            |:..|..|.:|      :|.|.:  :|||...:..|::.|...::.....|..
  Rat   929 SLVRKEVLKKVPDPALEESFQQLQQTLAEEQHKNQLLQEELGALQLRLQALEQ------------ 981

  Fly  1681 IADRNSGDKFRLDSAIANQLIDASVREVV-----DAKRDTKITLQEALQSGVLNAKTGRYVNEVT 1740
             ..|:.|.::             .|:||:     .|:.|..:.|:|.|: |:...|..|....:.
  Rat   982 -ETRDGGQEY-------------VVKEVLRIEPDRAQEDEVLQLREELE-GLRRQKGAREAEVLL 1031

  Fly  1741 KEKLTFAEARNRQLIVKPYTLKDICDLNLLDKQAQITSPMRR----------------EKLSIMQ 1789
            .::...|.|..:..:.:..|.:::..   |....|:.:..||                |:||.:|
  Rat  1032 LQQRVAALAAEKSRVQEKVTEREVVK---LQNDPQLEAEYRRLQEEHQREGTLREKQEEELSFLQ 1093

  Fly  1790 AIEAGVLDGNLLKCITKRKGELVTLQEAIADGIVLPAECKYRDFMTGELISIPEA--VERGLISS 1852
            |         .|:.:.|        :.|:|:|                .|::.|.  ||:   .:
  Rat  1094 A---------KLRRLEK--------ERAMAEG----------------KITVKEVLKVEK---DT 1122

  Fly  1853 VAQRSIFDID-GFKDLRSNDYVSFNVALSRDLLRRKSTGFALETGRDSLVPLEVAVSEGLVRQEV 1916
            .|:|.:.|:. .::|..|....|...  ..:|||:.   :|||.....:|          |:::|
  Rat  1123 AAEREVNDLSRQYEDEASKARTSQRE--KTELLRKI---WALEEENAKVV----------VQEKV 1172

  Fly  1917 YEMFSRGIGVQNASGKELSVFDLVYHNLIDPKTGYLLDPKT-GETVPLDTAI---ERKFITPEGA 1977
            .|                         ::.|      |||. .|...|...:   ||||...|..
  Rat  1173 RE-------------------------IVRP------DPKAESEVANLRLELVEQERKFRGAEEQ 1206

  Fly  1978 LL-----LSSLLN--ITLTTETVTRTINRYVTIRAGSQEPVDSVLLTFTEAVRQGFIDEERQLFK 2035
            |.     |.:|.|  ..:..:.||:.:.:|.|.....||         .:.:|:..:|:.|.:.:
  Rat  1207 LKSYQSELEALRNRGPQVEVKEVTKEVIKYTTDPETEQE---------LQRLREEIMDKTRLIER 1262

  Fly  2036 DPKTGNIYSVQQALNYGLLVPDSNQTVPEPTNRKKTKSTITIVTKQIIPEAEPIKLNTQHTKYVE 2100
            ...  .||.::|.:.   ::.|   |.|:            :.|::::.|....:.:.|..|.||
  Rat  1263 CDL--EIYQLKQEIQ---ILKD---TKPQ------------VQTREVVQEILQFQEDPQTKKEVE 1307

  Fly  2101 KSVEIPISQELVKPHRVVSEFINLEKSSYIEQNVTERQIMELPPGGWRL-KDAIEQRLFNPDTGV 2164
             |:.|.:|:|..|       .::||.....::...:|:..||..|..|: :..:.|....||...
  Rat  1308 -SLRIQLSEEKKK-------LVDLEGEQASQEEKIKRKEEELAQGKERIVRQEVVQYEDEPDLRA 1364

  Fly  2165 FHVQGTDRLVNFEECINKQIINNLSLSVIDPNTGDKISVQ--------SAFERDILDSYGNYTNS 2221
            .....||.:             ::.|..|     ||:.|:        :..||.:       ...
  Rat  1365 EVTAFTDSI-------------DVELRQI-----DKLCVELRRLQHRRAELERQL-------EEL 1404

  Fly  2222 RKQVQGMRSAIDESKIILETVPATRGANQKTILRITKVNNIPDVLEVSTPLKDAPPKFV------ 2280
            .::.|..|:|..|.:.:.:.:.|......||..::|....:  ||: ..|.:......:
  Rat  1405 ERERQARRAAELEVQRLQQRLAALEQEEAKTGEKVTHTQKV--VLQ-QDPQQTREHALLRAQLEE 1466

  Fly  2281 ----------EVLTCQRELASPEPLQIAPGAIYDPSTALVIFTQTGETENIFDAARQGLVDEQLI 2335
                      |:...:|:||:.|..:|....::..|    :..:.|:||......::.|.:|...
  Rat  1467 ERHRRQLLEGELEPLRRKLAALEKTEIKEKVVFSES----VQVEKGDTEQEIQRLKKSLEEESRS 1527

  Fly  2336 K--IVDPTTKQPISVTEAIARSIYDPKTATILD----------SEGQPVDLITATKLGLLSVVGA 2388
            |  :....|:....::|.   ..|:.|::..||          .|.|.:.|.|.
  Rat  1528 KRELDSEVTRLEAKLSEL---EFYNSKSSKELDFLREENHKLQLERQNLQLETR----------- 1578

  Fly  2389 PLVAAEGALRTVRFVTDPRTGEQIPVEVAYERGIVSRDQLHRGRSFDSEPATVEDKVVVLQRMRK 2453
                              |...:|.:.....|.:.:...:..|...:|...::|.::..|::|.
  Rat  1579 ------------------RLQSEIEMAATETRDLKNITMVDSGTHLNSRLWSLEKELDDLKKMS- 1624

  Fly  2454 VILKPKDALRKGIIDEETCEILENTSNFTNPKGEVVNITEALNTGLIDGRRGQVIDPQNNRVLNL 2518
               |.||.    .|||                              :..|.|.|...:..|..:|
  Rat  1625 ---KDKDL----EIDE------------------------------LQRRLGSVAVKREQRENHL 1652

  Fly  2519 RQAVEQKIIDSEQTNHILMPLAKSLSVPRLADQGLIDPQTQTIVHPESGYPLSIHEAIVCEVLD- 2582
            |:::                                     .::.|::|..||..||....::|
  Rat  1653 RRSI-------------------------------------VVIDPDTGRELSPEEAHRAGLIDW 1680

  Fly  2583 -PHSKLHKPEKCTLEEAITKGIINADDSTFNYK--NKTLNITEAIEAKLFDPS-YDQQKSK 2639
             ...|| :.::|..||...|| .|.:.|..:.:  .|..:|.:|:::....|: ||:..:|
  Rat  1681 KMFVKL-RSQECDWEEISVKG-PNGESSVIHDRKSGKKFSIEDALQSGRLTPAQYDRYVNK 1739

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
shot	NP_725339.1	SAC6	204..>420	CDD:227401
		CH_PLEC-like_rpt2	223..327	CDD:409038
		SPEC	471..689	CDD:238103	53/227 (23%)
		SPEC	690..866	CDD:238103	67/176 (38%)
		SH3_10	864..929	CDD:407754	20/65 (31%)
		SPEC	1047..1250	CDD:238103	42/210 (20%)
		SPEC	1153..1390	CDD:238103	53/248 (21%)
		PLEC	<1498..1526	CDD:197605	3/27 (11%)
		Plectin	1949..1980	CDD:459901	11/39 (28%)
		DUF5585	2860..>3198	CDD:465521
		SMC_prok_B	<4936..>5564	CDD:274008
		SPEC	5304..5513	CDD:238103
		SPEC	<5658..5787	CDD:413338
		SPEC	5793..6007	CDD:238103
		SPEC	6011..6220	CDD:238103
		SPEC	6118..6330	CDD:238103
		PRK09039	<6279..6441	CDD:181619
		SPEC	6443..6660	CDD:238103
		SPEC	6663..6878	CDD:238103
		SPEC	6880..7087	CDD:238103
		SPEC	6986..7196	CDD:238103
		SPEC	7196..7417	CDD:238103
		SPEC	7314..7526	CDD:238103
		SPEC	7528..7747	CDD:238103
		SPEC	7749..7968	CDD:238103
		SPEC	7973..8181	CDD:238103
		FRQ1	<8375..8444	CDD:444056
		GAS2	8450..8522	CDD:128539
		PHA03307	8550..>8795	CDD:223039
Ppl	NP_001100446.1	SPEC	213..387	CDD:413338	65/173 (38%)
		SH3_10	388..451	CDD:407754	20/65 (31%)
		Smc	<583..>1134	CDD:440809	146/789 (19%)
		SMC_prok_B	902..1638	CDD:274008	177/994 (18%)
		PLEC	<1655..1683	CDD:197605	7/64 (11%)