Protein Alignment Uba3 and UBA3

DIOPT Version :10

• Sequence 1: NP_610913.1
• Gene: Uba3 / 36539
• FlyBaseID: FBgn0263697
• Length: 450
• Species: Drosophila melanogaster
• Sequence 2: NP_003959.3
• Gene: UBA3 / 9039
• HGNCID: 12470
• Length: 463
• Species: Homo sapiens
• Alignment Length: 431
• Identity: 277/431 - (64%)
• Similarity: 344/431 - (79%)
• Gaps: 6/431 - (1%)

  Fly    16 RFNGLRNILEREGPFCKDGFAASSENLEFLQTKCQVLIIGAGGLGCELLKDLALMGFGNLHVIDM 80
            |:|.::..|||.|||....|..|:|:|:||...|:||:||||||||||||:|||.||..:|||||
Human    37 RWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDM 101

  Fly    81 DTIELSNLNRQFLFRRTDIGASKAECAARFINARVPTCRVTPHFKKIQDFDESFYQQFHLVVCGL 145
            |||::||||||||||..|||..|||.||.|:|.|||.|.|.|||.|||||:::||:|||::||||
Human   102 DTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGL 166

  Fly   146 DSIVARRWINGMLLSMLRYEEDGTIDTSSIVPMIDGGTEGFKGNARVILPGFTACIECTLDLFPP 210
            |||:|||||||||:|:|.| |||.:|.|||||:||||||||||||||||||.||||||||:|:||
Human   167 DSIIARRWINGMLISLLNY-EDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPP 230

  Fly   211 QVNYPLCTIANTPRLPEHCIEYVKIIQWEKQNPF--GVPLDGDDPQHIGWIYERALERSNEFNIT 273
            |||:|:||||:.|||||||||||:::||.|:.||  |||||||||:||.||::::|||::::||.
Human   231 QVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIR 295

  Fly   274 GVTYRLVQGVVKHIIPAVASTNAAIAAACALEVFKLATSCYDSMANYLNFNDLDGIYTYTYEAEK 338
            ||||||.|||||.||||||||||.|||.||.||||:|||.|..:.|||.|||:||:||||:|||:
Human   296 GVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAER 360

  Fly   339 SENCLACSNTPQPLPIEDPNTTTLEDVIKLLCDSPRFQLKSPALTTVMKDGKRRTLYMSGVKSIE 403
            .|||.|||..||  .|:...:..|::|:..|.:|...|:||||:|..: :||.||||:..|.|||
Human   361 KENCPACSQLPQ--NIQFSPSAKLQEVLDYLTNSASLQMKSPAITATL-EGKNRTLYLQSVTSIE 422

  Fly   404 EATRKNLTQSLGELGLHDGQQLTVTDATSPSAMTLQLKYQS 444
            |.||.||:::|.||||.|||:|.|.|.|:|..:..:|.:.|
Human   423 ERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS 463

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Uba3	NP_610913.1	Uba3_RUB	50..346	CDD:238765	217/297 (73%)
		E2_bind	357..442	CDD:462611	39/84 (46%)
UBA3	NP_003959.3	Interaction with UBE2M N-terminus	53..70		6/16 (38%)
		Uba3_RUB	71..368	CDD:238765	217/297 (73%)
		Interaction with UBE2M N-terminus	157..161		2/3 (67%)
		Interaction with UBE2M N-terminus	192..217		22/24 (92%)
		Interaction with NEDD8	227..229		1/1 (100%)
		Interaction with NAE1. /evidence=ECO:0000269|PubMed:12740388	242..248		4/5 (80%)
		Interaction with NAE1. /evidence=ECO:0000269|PubMed:12740388	292..295		1/2 (50%)
		Interaction with UBE2M N-terminus	331..338		4/6 (67%)
		Interaction with NEDD8	352..357		4/4 (100%)
		Interaction with UBE2M core domain	368..463		43/97 (44%)
		E2_bind	376..461	CDD:462611	39/85 (46%)