Protein Alignment Polr3B and RPA135

DIOPT Version :10

• Sequence 1: NP_523706.1
• Gene: Polr3B / 36289
• FlyBaseID: FBgn0004463
• Length: 1137
• Species: Drosophila melanogaster
• Sequence 2: NP_015335.1
• Gene: RPA135 / 856119
• SGDID: S000006214
• Length: 1203
• Species: Saccharomyces cerevisiae
• Alignment Length: 1277
• Identity: 367/1277 - (28%)
• Similarity: 567/1277 - (44%)
• Gaps: 272/1277 - (21%)

  Fly    28 IKPLTEKWKLVPAFLQVKGLVKQHIDSFN--------HFINVDIK----KIV-------KANELV 73
            |.|..:|    .||..::..|:.||.|||        ..:|:.:|    |::       ..:|:.
Yeast    26 INPPKDK----SAFPLLQEAVQPHIGSFNALTEGPDGGLLNLGVKDIGEKVIFDGKPLNSEDEIS 86

  Fly    74 TSGADPLFYLKYL---------DVRVGKPDIDDGFN--ITKATTPHECRLRDTTYSAPITVDIEY 127
            .||        ||         .|.:.||..:||.:  :.:...|.|.|.|.|:|...:.:.:::
Yeast    87 NSG--------YLGNKLSVSVEQVSIAKPMSNDGVSSAVERKVYPSESRQRLTSYRGKLLLKLKW 143

  Fly   128 T--RGTQRIKRNNLL----IGRMPLMLRCSNCALTGKSEFELSKLNECPLDPGGYFVVRGQEKVI 186
            :  .|.:     ||.    .|.:|:||:.:.|.|...|.:||.:..|...:.||||:|.|.||:|
Yeast   144 SVNNGEE-----NLFEVRDCGGLPVMLQSNRCHLNKMSPYELVQHKEESDEIGGYFIVNGIEKLI 203

  Fly   187 -------------LIQEQLSWNKMLTEDFNGVVQCQVTSSTHEKKSRTLVLSKHGKYYLKHNSMT 238
                         :|:...: |:..:....|:   |:.|...::.|:|.||     :||....:|
Yeast   204 RMLIVQRRNHPMAIIRPSFA-NRGASYSHYGI---QIRSVRPDQTSQTNVL-----HYLNDGQVT 259

  Fly   239 DD---------IPIVVIFKALGVVSDQEI-QSLIGIDSKSQNRFGASLIDAYNL----------K 283
            ..         :|:|:|.|||...||:|| ..:||.|.|.     :.|.|...|          .
Yeast   260 FRFSWRKNEYLVPVVMILKALCHTSDREIFDGIIGNDVKD-----SFLTDRLELLLRGFKKRYPH 319

  Fly   284 VFTQQRALEYMGSKLVVKRFQSATTKTPSEEARELLLTTILAHVPVDNFNLQMKAIYVSMMVRRV 348
            :..:.:.|:|:|.|..|. ||::..::..|..:|:|...:|.|:..|  ..|.|...:..|:|::
Yeast   320 LQNRTQVLQYLGDKFRVV-FQASPDQSDLEVGQEVLDRIVLVHLGKD--GSQDKFRMLLFMIRKL 381

  Fly   349 MAAELDKTLFDDRDYYGNKRLELAGSLLSMMFEDLFKRMNWELKTIADKNIPKVKAAQFDVVKHM 413
            .:....:...|:.|...::.:.|.|.|..|:.::........:......:|.:..|..|...::|
Yeast   382 YSLVAGECSPDNPDATQHQEVLLGGFLYGMILKEKIDEYLQNIIAQVRMDINRGMAINFKDKRYM 446

  Fly   414 RAA------QITAGLESAISSGNWTIKR-FKMER-AGVTQVLSRLS---YISALGMMTRVNSQFE 467
            ...      .|.:.::..:|:||...:. ..::: :|.|.|..:::   :||...|:.|  ..|.
Yeast   447 SRVLMRVNENIGSKMQYFLSTGNLVSQSGLDLQQVSGYTVVAEKINFYRFISHFRMVHR--GSFF 509

  Fly   468 KTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTEVEE-RPVMIVAFNAGVEDIR 531
            ...|.:..|.|.|..||.|||..||:|..|||:.:.|....|:|:..: ..:..:.::.||   .
Yeast   510 AQLKTTTVRKLLPESWGFLCPVHTPDGSPCGLLNHFAHKCRISTQQSDVSRIPSILYSLGV---A 571

  Fly   532 EVSGNPINNPNVFLVFINGNVLGLTLNH---KHLVRNLRYMRRKGRMG--------SYVSVHTSY 585
            ..|......|::..|.|:|.::| .::|   |.:...|||.:.:|:..        .||...|..
Yeast   572 PASHTFAAGPSLCCVQIDGKIIG-WVSHEQGKIIADTLRYWKVEGKTPGLPIDLEIGYVPPSTRG 635

  Fly   586 TQRCIYIHTDGGRLCRPYVIVENRRPLVKQHHLDELNRGIRKFDDFLLDGLIEYLDVNEENDSFI 650
            ....:|:.....|:.||          |:...||:        :|.:  |..|.:.:|      |
Yeast   636 QYPGLYLFGGHSRMLRP----------VRYLPLDK--------EDIV--GPFEQVYMN------I 674

  Fly   651 AWNEDQIEDRT-THLEIEPFTLLGVCAGLVPYPHHNQSPRNTYQCAMGKQAMGMIGYNQKNRIDS 714
            |....:|::.. ||:|..|..:|.:.|.|.|:...||||||.|||.||||.||..|....:|.|:
Yeast   675 AVTPQEIQNNVHTHVEFTPTNILSILANLTPFSDFNQSPRNMYQCQMGKQTMGTPGVALCHRSDN 739

  Fly   715 LMYNLVYPHAPMVKSKTIELTNFDKLPAGQNATVAVMSYSGYDIEDALILNKASIDRGYGRCLVY 779
            .:|.|.....|:||:...:....|..|.|.||.|||:||:|||::||:|:||::.:||:|...:|
Yeast   740 KLYRLQTGQTPIVKANLYDDYGMDNFPNGFNAVVAVISYTGYDMDDAMIINKSADERGFGYGTMY 804

  Fly   780 KNSKCTVKRYANQTFDRIMGPMKDALTNKVIFKHD--------VLDTDGIVAPGEQVQNKQIM-- 834
            |..|..:....|:         .|.:|....|.:|        .||.||:...|..|:....:
Yeast   805 KTEKVDLALNRNR---------GDPITQHFGFGNDEWPKEWLEKLDEDGLPYIGTYVEEGDPICA 860

  Fly   835 -----INKEMPAVTSMNPLQGQSAQVPYTAVPISYKGPEPSYIERVMVSANAEEDF----LIKIL 890
                 :||                    |.:. :|...||:|||.|.:..:....|    .:.|.
Yeast   861 YFDDTLNK--------------------TKIK-TYHSSEPAYIEEVNLIGDESNKFQELQTVSIK 904

  Fly   891 LRQTRIPEIGDKFSSRHGQKGVTGLIVEQEDMPFNDFGICPDMIMNPHGFPSRMTVGKTLELLGG 955
            .|..|.|:||||||||||||||........||||::.||.||:|:|||.||||||:|..:|.|.|
Yeast   905 YRIRRTPQIGDKFSSRHGQKGVCSRKWPTIDMPFSETGIQPDIIINPHAFPSRMTIGMFVESLAG 969

  Fly   956 KAGLLEGKFHYGTAFGGSKVEDIQA-----ELERHGFNYVGKDFFYSGITGTPLEAYIYSGPVYY 1015
            |||.|.|.....|.:..:: :|..|     :|.:.|:||.|.:..|||.||..|.|.||.|.|||
Yeast   970 KAGALHGIAQDSTPWIFNE-DDTPADYFGEQLAKAGYNYHGNEPMYSGATGEELRADIYVGVVYY 1033

  Fly  1016 QKLKHMVQDKMHARARGPKAVLTRQPTQGRSREGGLRLGEMERDCLISYGASMLIMERLMISSDA 1080
            |:|:|||.||...|:.||...||.||.:||.|.||:|:||||||.||.:|.|.|:.:||:.|||.
Yeast  1034 QRLRHMVNDKFQVRSTGPVNSLTMQPVKGRKRHGGIRVGEMERDALIGHGTSFLLQDRLLNSSDY 1098

  Fly  1081 FEVDVCRTCGRM--------------AYCSWCHFCQSSANVSK---------------------- 1109
            .:..|||.||.:              ..|  |..|......:|
Yeast  1099 TQASVCRECGSILTTQQSVPRIGSISTVC--CRRCSMRFEDAKKLLTKSEDGEKIFIDDSQIWED 1161

  Fly  1110 --------------ISMPYACKLLFQELTSM------NVVPK 1131
                          :::|:..|.|..||::|      ||.||
Yeast  1162 GQGNKFVGGNETTTVAIPFVLKYLDSELSAMGIRLRYNVEPK 1203

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Polr3B	NP_523706.1	RNA_pol_B_RPB2	47..1133	CDD:238353	362/1258 (29%)
		RNA_pol_Rpb2_4	546..607	CDD:428012	17/71 (24%)
		RNA_pol_Rpb2_5	628..668	CDD:428013	10/40 (25%)
RPA135	NP_015335.1	RNA_pol_B_RPB2	42..1199	CDD:238353	358/1251 (29%)
		RNA_pol_Rpb2_2	210..403	CDD:398318	48/209 (23%)
		RNA_pol_Rpa2_4	593..651	CDD:462028	12/58 (21%)