Protein Alignment Polr3B and RPB2

DIOPT Version :10

• Sequence 1: NP_523706.1
• Gene: Polr3B / 36289
• FlyBaseID: FBgn0004463
• Length: 1137
• Species: Drosophila melanogaster
• Sequence 2: NP_014794.3
• Gene: RPB2 / 854322
• SGDID: S000005677
• Length: 1224
• Species: Saccharomyces cerevisiae
• Alignment Length: 1231
• Identity: 438/1231 - (35%)
• Similarity: 673/1231 - (54%)
• Gaps: 143/1231 - (11%)

  Fly    17 DPGDSKDWSVPIKPLTEKWKLVPAFLQVKGLVKQHIDSFNHFINVDIKKIVKANELV-------- 73
            ||...:|.|.|| ...:.|.::.||.:.||||.|.:||||.|::..::.|:..:..:
Yeast    14 DPYGFEDESAPI-TAEDSWAVISAFFREKGLVSQQLDSFNQFVDYTLQDIICEDSTLILEQLAQH 77

  Fly    74 TSGADPL---FYLKYLDVRVGKPDIDDGFNITKATTPHECRLRDTTYSAPITVDIEYTRGTQRI- 134
            |:.:|.:   :.:.:..:.|.||.:::...:|.|..|.|.|||:.|||:.:.||:: .|..:.|
Yeast    78 TTESDNISRKYEISFGKIYVTKPMVNESDGVTHALYPQEARLRNLTYSSGLFVDVK-KRTYEAID 141

  Fly   135 -------------------KRNNLLIGRMPLMLRCSNCALTGKSEFELSKLNECPLDPGGYFVVR 180
                               :...:.|||:|:|||..||.|:..:|.:|.||.|||.|.||||::.
Yeast   142 VPGRELKYELIAEESEDDSESGKVFIGRLPIMLRSKNCYLSEATESDLYKLKECPFDMGGYFIIN 206

  Fly   181 GQEKVILIQEQLSWNKMLTEDFNGVVQCQVT-----SSTHEKKSR---TLVLSKHGKY------- 230
            |.|||::.||:.:.|  :.:.|.......::     .|..||.||   ||.:..:|:.
Yeast   207 GSEKVLIAQERSAGN--IVQVFKKAAPSPISHVAEIRSALEKGSRFISTLQVKLYGREGSSARTI 269

  Fly   231 -----YLKHNSMTDDIPIVVIFKALGVVSDQEIQSLIGIDS---KSQNRFGASLIDAYNLKVFTQ 287
                 |:|     .|||||:||:|||::.|.||...|..|.   :........:.|.:.::  .:
Yeast   270 KATLPYIK-----QDIPIVIIFRALGIIPDGEILEHICYDVNDWQMLEMLKPCVEDGFVIQ--DR 327

  Fly   288 QRALEYMGSK---LVVKRFQSATTKTPSEEARELLLTTILAHV-PVDNFNLQMKAIYVSMMVRRV 348
            :.||:::|.:   |.:|:      :...:.|:::|....|.|: .::.|. ..||.::..|:.|:
Yeast   328 ETALDFIGRRGTALGIKK------EKRIQYAKDILQKEFLPHITQLEGFE-SRKAFFLGYMINRL 385

  Fly   349 MAAELDKTLFDDRDYYGNKRLELAGSLLSMMFEDLFKRMNWELKTIADKNIPKVKAAQFDVVKHM 413
            :...||:...||||::|.|||:|||.||:.:|:.|||::..::.....:.:.  :|..|::...:
Yeast   386 LLCALDRKDQDDRDHFGKKRLDLAGPLLAQLFKTLFKKLTKDIFRYMQRTVE--EAHDFNMKLAI 448

  Fly   414 RAAQITAGLESAISSGNW-TIKRFKMERAGVTQVLSRLSYISALGMMTRVNSQFEKTRKVSGPRS 477
            .|..||:||:.|:::||| ..|:....||||:|||:|.:|.|.|..:.|.|:...:..|::.||.
Yeast   449 NAKTITSGLKYALATGNWGEQKKAMSSRAGVSQVLNRYTYSSTLSHLRRTNTPIGRDGKLAKPRQ 513

  Fly   478 LQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTEVEERPVMIVAFNAGVEDIREVSGNPINNPN 542
            |..:.||::||::||||:||||||||:||:.|:...:..|::......|:|.:.:..  |..:|:
Yeast   514 LHNTHWGLVCPAETPEGQACGLVKNLSLMSCISVGTDPMPIITFLSEWGMEPLEDYV--PHQSPD 576

  Fly   543 VFLVFINGNVLGLTLNHKHLVRNLRYMRRKGRMGSYVSVHTSYTQRCIYIHTDGGRLCRPYVIVE 607
            ...||:||...|:..|...|:..||.:||||.:...||:.....::.:.|.||.||:.||..|||
Yeast   577 ATRVFVNGVWHGVHRNPARLMETLRTLRRKGDINPEVSMIRDIREKELKIFTDAGRVYRPLFIVE 641

  Fly   608 NRRPL------VKQHHL--------DELNRGIRKFDDF-----LLDGLIEYLDVNEENDSFIAWN 653
            :...|      |::.|:        .::..|....:::     |.:||:||:|..||....||..
Yeast   642 DDESLGHKELKVRKGHIAKLMATEYQDIEGGFEDVEEYTWSSLLNEGLVEYIDAEEEESILIAMQ 706

  Fly   654 EDQIEDRT------------------------THLEIEPFTLLGVCAGLVPYPHHNQSPRNTYQC 694
            .:.:|...                        ||.||.|..:|||.|.::|:|.||||||||||.
Yeast   707 PEDLEPAEANEENDLDVDPAKRIRVSHHATTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQS 771

  Fly   695 AMGKQAMGMIGYNQKNRIDSLMYNLVYPHAPMVKSKTIELTNFDKLPAGQNATVAVMSYSGYDIE 759
            ||||||||:...|...|:|::...|.||..|:..::.:|...|.:|||||||.||:..||||:.|
Yeast   772 AMGKQAMGVFLTNYNVRMDTMANILYYPQKPLGTTRAMEYLKFRELPAGQNAIVAIACYSGYNQE 836

  Fly   760 DALILNKASIDRGYGRCLVYKNSKCTVKRYA---NQTFDRIMGPMKDALTNKVIFKH---DVLDT 818
            |::|:|::|||||..|.|.:::.....|:|.   .:||::   |.:   ||.:..||   |.||.
Yeast   837 DSMIMNQSSIDRGLFRSLFFRSYMDQEKKYGMSITETFEK---PQR---TNTLRMKHGTYDKLDD 895

  Fly   819 DGIVAPGEQVQNKQIMINKEMPAVTSMNPLQGQSAQVPYTAVPISYKGPEPSYIERVMVSANAEE 883
            ||::|||.:|..:.::|.|..|.......|..::|...........:..|...:::|:|:.|.:.
Yeast   896 DGLIAPGVRVSGEDVIIGKTTPISPDEEELGQRTAYHSKRDASTPLRSTENGIVDQVLVTTNQDG 960

  Fly   884 DFLIKILLRQTRIPEIGDKFSSRHGQKGVTGLIVEQEDMPFNDFGICPDMIMNPHGFPSRMTVGK 948
            ...:|:.:|.|:||:|||||:|||||||..|:...:|||||...||.||:|:|||..||||||..
Yeast   961 LKFVKVRVRTTKIPQIGDKFASRHGQKGTIGITYRREDMPFTAEGIVPDLIINPHAIPSRMTVAH 1025

  Fly   949 TLELLGGKAGLLEGKFHYGTAFGGSKVEDIQAELERHGFNYVGKDFFYSGITGTPLEAYIYSGPV 1013
            .:|.|..|...|.|.....:.|....||.|...|..||:...|.:..|:|.||..|.|.|:.||.
Yeast  1026 LIECLLSKVAALSGNEGDASPFTDITVEGISKLLREHGYQSRGFEVMYNGHTGKKLMAQIFFGPT 1090

  Fly  1014 YYQKLKHMVQDKMHARARGPKAVLTRQPTQGRSREGGLRLGEMERDCLISYGASMLIMERLMISS 1078
            |||:|:|||.||:|||||||..||||||.:||||:||||.|||||||:|::||:..:.||||.:|
Yeast  1091 YYQRLRHMVDDKIHARARGPMQVLTRQPVEGRSRDGGLRFGEMERDCMIAHGAASFLKERLMEAS 1155

  Fly  1079 DAFEVDVCRTCGRMAYCS-------WCHFCQSSANVSKISMPYACKLLFQELTSMNVVPKM 1132
            |||.|.:|..||.|...:       .|..|.:..::.:|.:|||.|||||||.:||:.|::
Yeast  1156 DAFRVHICGICGLMTVIAKLNHNQFECKGCDNKIDIYQIHIPYAAKLLFQELMAMNITPRL 1216

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Polr3B	NP_523706.1	RNA_pol_B_RPB2	47..1133	CDD:238353	427/1201 (36%)
		RNA_pol_Rpb2_4	546..607	CDD:428012	23/60 (38%)
		RNA_pol_Rpb2_5	628..668	CDD:428013	15/68 (22%)
RPB2	NP_014794.3	Ricin_B_lectin	31..1216	CDD:481363	432/1211 (36%)