Protein Alignment en and Nanog

DIOPT Version :10

• Sequence 1: NP_523700.2
• Gene: en / 36240
• FlyBaseID: FBgn0000577
• Length: 552
• Species: Drosophila melanogaster
• Sequence 2: NP_082292.1
• Gene: Nanog / 71950
• MGIID: 1919200
• Length: 305
• Species: Mus musculus
• Alignment Length: 196
• Identity: 56/196 - (28%)
• Similarity: 81/196 - (41%)
• Gaps: 61/196 - (31%)

  Fly   325 SLASPPPASNASTISSTSSV-------ATSSSSSSSGCSSAASSLNSSPSSR-LGASGSGVNASS 381
            ||.|...|||:...||..:|       ....|::...|:.|||   ..|||. |...||..:::|
Mouse    10 SLPSSEEASNSGNASSMPAVFHPENYSCLQGSATEMLCTEAAS---PRPSSEDLPLQGSPDSSTS 71

  Fly   382 PQPQPIPPPSAVSRDSGMESSDDTRSETGSTTTEGGKNEMWPAWVYCTRYSDRPSSGPRYRRPKQ 446
            |: |.:..|.|   |.|                                             |::
Mouse    72 PK-QKLSSPEA---DKG---------------------------------------------PEE 87

  Fly   447 PKDKTNDEK-RPRTAFSSEQLARLKREFNENRYLTERRRQQLSSELGLNEAQIKIWFQNKRAKIK 510
            .::|....| :.||.||..||..||..|.:.:||:.::.|:|||.|.|:..|:|.||||:|.|.|
Mouse    88 EENKVLARKQKMRTVFSQAQLCALKDRFQKQKYLSLQQMQELSSILNLSYKQVKTWFQNQRMKCK 152

  Fly   511 K 511
            :
Mouse   153 R 153

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
en	NP_523700.2	Homeodomain	455..511	CDD:459649	26/56 (46%)
		Engrail_1_C_sig	512..542	CDD:431338	56/196 (29%)
Nanog	NP_082292.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..30		8/19 (42%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	46..95		19/100 (19%)
		Sufficient for interaction with SALL4. /evidence=ECO:0000269|PubMed:16840789	96..155		27/58 (47%)
		Homeodomain	98..153	CDD:459649	25/54 (46%)
		Required for DNA-binding. /evidence=ECO:0000250|UniProtKB:Q9H9S0	123..152		14/28 (50%)
		PTZ00395	<190..>250	CDD:185594
		10 X repeats starting with a Trp in each unit	198..247
		Sufficient for transactivation activity	198..247
		Sufficient for strong transactivation activity	248..305