Protein Alignment piwi and PIWIL1

DIOPT Version :10

• Sequence 1: NP_476875.1
• Gene: piwi / 34521
• FlyBaseID: FBgn0004872
• Length: 843
• Species: Drosophila melanogaster
• Sequence 2: NP_004755.2
• Gene: PIWIL1 / 9271
• HGNCID: 9007
• Length: 861
• Species: Homo sapiens
• Alignment Length: 860
• Identity: 312/860 - (36%)
• Similarity: 485/860 - (56%)
• Gaps: 68/860 - (7%)

  Fly     6 GRGRRRPLNEDDSSTSRGSGDGPRVKVFRGSSSGDPRADPRIEASRERRALEEAPRREGGPTERK 70
            ||||:|       .|:.|:.....:::..|                    .:|....|.|...|
Human    48 GRGRQR-------GTAGGTAKSQGLQISAG--------------------FQELSLAERGGRRR- 84

  Fly    71 PWGDQYDY-LNTRP-AELV--SKKGTDGVPVMLQTNFFRLKTKPEWRIVHYHVEFEPSIENPRVR 131
               |.:|. :|||. .:.|  ||.|:.|:.|.|.||.|||.::|:|.:..||:::.|.:|..|:|
Human    85 ---DFHDLGVNTRQNLDHVKESKTGSSGIIVRLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLR 146

  Fly   132 MGVLSNHANLLGSGYLFDGLQLFTTRKFEQEITVLSGKSKLDIEYKISIKFVGFISCAEPRFLQV 196
            ..:|..|.:|:|..:.|||..||..::.:|::|.:..|::...:.:|:|.....:....|..||.
Human   147 SALLFQHEDLIGKCHAFDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCLQF 211

  Fly   197 LNLILRRSMKGLNLELVGRNLFDPRAKIEIREFKMELWPGYETSIRQHEKDILLGTEITHKVMRT 261
            .|:|.||.:|.:||:.:|||.::|...|:|...::.:|||:.|||.|:|..|:|.|:::|||:|:
Human   212 YNIIFRRLLKIMNLQQIGRNYYNPNDPIDIPSHRLVIWPGFTTSILQYENSIMLCTDVSHKVLRS 276

  Fly   262 ETIYDIMRRCSHNPARH--QDEVRVNVLDLIVLTDYNNRTYRINDVDFGQTPKSTF-SCKGRDIS 323
            ||:.|.|....|....|  |::|...::.|:|||.|||:|||::|:|:.|.||||| ...|.::|
Human   277 ETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVS 341

  Fly   324 FVEYYLTKYNIRIRDHNQPLLISK-NRDKALKTNASELVVLIPELCRVTGLNAEMRSNFQLMRAM 387
            |:|||..:||..|.|..||:|:|: .|.:..........:||||||.:|||..:||::|.:|:.:
Human   342 FLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDL 406

  Fly   388 SSYTRMNPKQR---TDRLRAFNHRLQNTPESVKVLRDWNMELDKNVTEVQGRIIGQQNIVFHNGK 449
            :.:||:.|:||   ..||..:.|:..|..   :.||||.:..|.|:....|||:..:.|  |.|.
Human   407 AVHTRLTPEQRQREVGRLIDYIHKNDNVQ---RELRDWGLSFDSNLLSFSGRILQTEKI--HQGG 466

  Fly   450 VPAGEN---ADWQRHFRDQRMLTTPSDGLDRWAVIAPQRNSHELRTLLDSLYRAASGMGLRIRSP 511
            .....|   |||.:..|...:::...  ||.|.:|..:||.....:|:.:|::....||:::|.
Human   467 KTFDYNPQFADWSKETRGAPLISVKP--LDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRK- 528

  Fly   512 QEFIIYDDRTGTYVRAMDDCVRSDPKLILCLVPNDNAERYSSIKKRGYVDRAVPTQVVTLKTT-K 575
            ...|..||||..|:|.:...|.:|.::::||:.::..::|.:|||....|...|:|.|..:|. |
Human   529 AIMIEVDDRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGK 593

  Fly   576 NRSLMSIATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDRKRAYGALIASMDLQQNST 640
            .:::|:||||||:|:|||:|...|.:::||..:|.:|.|........:|:....:||:: :..:.
Human   594 QQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVGIDCYHDMTAGRRSIAGFVASIN-EGMTR 657

  Fly   641 YFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVFYRDGVSSGSLKQLFEFEVKDIIE 705
            :||..........|.:.|...:..|||.:...:..:||||:.|||||..|.||.|..:||...::
Human   658 WFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLD 722

  Fly   706 KLKT---EYARVQLSPPQLAYIVVTRSMNTRFFLNG----QNPPPGTIVDDVITLPERYDFYLVS 763
            .||:   .|      .|:|..|||.:.:|||||...    |||.|||::|..:|.||.|||::||
Human   723 CLKSIGRGY------NPRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVS 781

  Fly   764 QQVRQGTVSPTSYNVLYSSMGLSPEKMQKLTYKMCHLYYNWSGTTRVPAVCQYAKKLATLVGTNL 828
            |.||.|:||||.|||:|.:.||.|:.:|:||||:||:||||.|..||||.||||.|||.|||.::
Human   782 QAVRSGSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAFLVGQSI 846

  Fly   829 HSIPQNALEKKFYYL 843
            |..|..:|..:.|||
Human   847 HREPNLSLSNRLYYL 861

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
piwi	NP_476875.1	PAZ	262..396	CDD:198017	56/137 (41%)
		Piwi_piwi-like_Euk	382..826	CDD:240016	170/457 (37%)
PIWIL1	NP_004755.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..64		7/22 (32%)
		GAGE	16..106	CDD:461753	20/88 (23%)
		D-box. /evidence=ECO:0000269|PubMed:28552346	217..224		3/6 (50%)
		PAZ	277..415	CDD:198017	56/137 (41%)
		Required for binding 2'-O-methylated 3'-end of piRNAs. /evidence=ECO:0000269|PubMed:21193640	316..318		1/1 (100%)
		Piwi_piwi-like_Euk	401..844	CDD:240016	170/457 (37%)
		MID region. /evidence=ECO:0000250|UniProtKB:Q9JMB7	479..615		45/138 (33%)