Protein Alignment piwi and Ago1

DIOPT Version :10

• Sequence 1: NP_476875.1
• Gene: piwi / 34521
• FlyBaseID: FBgn0004872
• Length: 843
• Species: Drosophila melanogaster
• Sequence 2: NP_001304110.1
• Gene: Ago1 / 313594
• RGDID: 1304619
• Length: 895
• Species: Rattus norvegicus
• Alignment Length: 868
• Identity: 198/868 - (22%)
• Similarity: 349/868 - (40%)
• Gaps: 185/868 - (21%)

  Fly    58 EAPRREGGPTERKPWGDQYDYLNTRPAELVSKKGTDGVPVMLQTNFFRLKTKPEWRIVHYHVEFE 122
            :||||.|                         .||.|.|:.|..|:|.:.. |:..:.||.|:.:
  Rat    22 QAPRRPG-------------------------IGTVGKPIKLLANYFEVDI-PKIDVYHYEVDIK 60

  Fly   123 PSIENPRVRMGVLSNHANLLGSGYLFDGLQLFTTRKFEQEITVLS-GKSKLDIE----------- 175
            |.....||...|:.............|...::..:|....:|.|. |..::|.|
  Rat    61 PDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRI 125

  Fly   176 YKISIKFVGFISCAEPRFL----------------QVLNLILRRSMKGLNLELVGRNLFDP---- 220
            :|:|||::..:|.   |.|                |.|::.:|. :..:....|||:.|.|
  Rat   126 FKVSIKWLAIVSW---RMLHEALVSGQIPVPLESVQALDVAMRH-LASMRYTPVGRSFFSPPEGY 186

  Fly   221 -------RAKIEIREFKMELWPGYETSIRQHEKDILLGTEITHKVM-RTETIYDIM------RRC 271
                   |          |:|.|:..|:|.....::|..:::.... :.:.:.:.|      |..
  Rat   187 YHPLGGGR----------EVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNI 241

  Fly   272 SHNPARHQDEVRV----NVLDLIVLTDY---NNRTYRINDVDFGQTPKSTFSCK---GRDI--SF 324
            ...|....|..||    .:..|.|...:   ..|.||:.:|........||..:   |:.:  :.
  Rat   242 DEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTV 306

  Fly   325 VEYYLTKYNIRIRDHNQPLLISKNRDKALKTNASELVVLIP-ELCRVTGLNAEMRSNFQLM-RAM 387
            .:|:..|||::::..:.|         .|:....:....:| |:|.:.   |..|...:|. ...
  Rat   307 AQYFKQKYNLQLKYPHLP---------CLQVGQEQKHTYLPLEVCNIV---AGQRCIKKLTDNQT 359

  Fly   388 SSYTRMNPKQRTDRLRAFNHRLQNTPESV-KVLRDWNMELDKNVTEVQGRIIGQQNIVFHNGKVP 451
            |:..:...:...||....:..::|...:: ..::::.:::..::|||.||:: ...|:.:.|:
  Rat   360 STMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVL-PAPILQYGGR-- 421

  Fly   452 AGENADWQRHFRDQRMLTTPSDG--------------LDRWAV--IAPQRNSHE--LRTLLDSLY 498
                         .|.:.||:.|              :..||:  .|||:...|  |:...|.|.
  Rat   422 -------------NRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLR 473

  Fly   499 RAASGMGLRIRSPQEFIIY---DDRTGTYVRAMDDCVRSDPKLILCLVPNDNAERYSSIKKRGYV 560
            :.:...|:.|:....|..|   .|......|.:.: ..|..:||:.::|. ....|:.:|:.|..
  Rat   474 KISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKN-TYSGLQLIIVILPG-KTPVYAEVKRVGDT 536

  Fly   561 DRAVPTQVVTLKTTKNRSLMSIATKIAIQLNCKLG-----YTPW----MIELPLSGLMTIGFDIA 616
            ...:.||.|.:|.....|..:: :.:.:::|.|||     ..|.    :.:.|   ::.:|.|:.
  Rat   537 LLGMATQCVQVKNVVKTSPQTL-SNLCLKINVKLGGINNILVPHQRSAVFQQP---VIFLGADVT 597

  Fly   617 --KSTRDRKRAYGALIASMDLQQNSTYFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSR 679
              .:...:|.:..|::.|||... |.|.:||........:...|..|:.:.|.|:....|..|:|
  Rat   598 HPPAGDGKKPSITAVVGSMDAHP-SRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTR 661

  Fly   680 IVFYRDGVSSGSLKQLFEFE---VKDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFFLNGQNP 741
            |:||||||..|.|.|:..:|   ::|...||:.:|      .|.:.||||.:..:||.|...:|.
  Rat   662 IIFYRDGVPEGQLPQILHYELLAIRDACIKLERDY------QPGITYIVVQKRHHTRLFCADKNE 720

  Fly   742 --------PPGTIVDDVITLPERYDFYLVSQQVRQGTVSPTSYNVLYSSMGLSPEKMQKLTYKMC 798
                    |.||.||..||.|..:||||.|....|||..|:.|.||:.....:.:::|.|||::|
  Rat   721 RIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLC 785

  Fly   799 HLYYNWSGTTRVPAVCQYAKKLA 821
            |.|...:.:..:||...||:.:|
  Rat   786 HTYVRCTRSVSIPAPAYYARLVA 808

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
piwi	NP_476875.1	PAZ	262..396	CDD:198017	28/153 (18%)
		Piwi_piwi-like_Euk	382..826	CDD:240016	123/485 (25%)
Ago1	NP_001304110.1	ArgoN	34..164	CDD:465134	28/133 (21%)
		ArgoL1	174..224	CDD:462567	12/59 (20%)
		PAZ_argonaute_like	225..345	CDD:239212	24/128 (19%)
		Piwi_ago-like	390..815	CDD:240015	118/448 (26%)