Protein Alignment Sur and cftr

DIOPT Version :10

• Sequence 1: NP_001334732.1
• Gene: Sur / 34350
• FlyBaseID: FBgn0028675
• Length: 2223
• Species: Drosophila melanogaster
• Sequence 2: NP_001038348.1
• Gene: cftr / 559080
• ZFINID: ZDB-GENE-050517-20
• Length: 1485
• Species: Danio rerio
• Alignment Length: 2023
• Identity: 421/2023 - (20%)
• Similarity: 738/2023 - (36%)
• Gaps: 662/2023 - (32%)

  Fly   226 ATFYSKSCFWWLTPLLWLGYKEPLELEDLGQMKLEDSA-------RSHYDHFLYIYTEKKKKSNS 283
            |...|:..|||..|::..|:||.|...|:.|...:|:|       ...:|..:   ...|||.:.
Zfish     9 ANCLSRYFFWWTNPIMRKGFKEKLRPSDVYQAPSQDAADILAERLEKEWDREV---ASGKKKPSL 70

  Fly   284 SPSLWYCYIKNSWQMFALGGILKLAGDLFALIGPLAIQKIVEYIEQLYAQASEPPAKSPGNEVAN 348
            ..::..||||    .|.|.|.|...|:....:.|..:.:|:         ||..||..|..
Zfish    71 LRAMARCYIK----PFLLFGFLLYIGEATKTVQPQLLGRII---------ASFDPAHEPER---- 118

  Fly   349 VLLSTSRILGTEFDEVFGTNIDKVRIYSSTWSDLLANGWCIAWIVLLAAITQGALSQASTHILNM 413
                                               |||:.:|:.:.|....:..|.|.:...|:.
Zfish   119 -----------------------------------ANGYFLAFGLGLLFTARFLLLQPAMFGLHH 148

  Fly   414 TGIRIKTSLQGLIYRKSLLLNA---DGGCDSSDSAGQVQSTSSTSDEKQKNDDSMATPEHVDNPS 475
            .|::|:.:|..:||:|:|.|::   |     ..|.||:.|..|.:  ..|.|.|:.
Zfish   149 LGMQIRIALFSIIYKKTLKLSSRVLD-----KISTGQLVSLMSAN--LGKFDQSLG--------- 197

  Fly   476 EPNISHDIGSITNHMTEDTRNIMEFFLIIHYAWAIPFKIAVVIYLLYMNLGISAVIGSIACIVIM 540
                                       :.|:.|..|.:..:...|::..:.:::.. ::|.|.::
Zfish   198 ---------------------------MAHFIWISPLQCILCTGLIWELIDVNSFC-ALAAISLL 234

  Fly   541 TPLQFFIGNAMS--KNAEVIAGYTDERLKRIHDTLVGIKVIKLNAWDEVFLKKIQEARRKE---- 599
            ..||.|:.:.|.  |..:|:  .|::||....:.:..:..:|...|:|:....|:..|:.|
Zfish   235 GVLQAFLSHKMGPYKAQKVL--LTNKRLALTSEIMENLHSVKAYGWEEIMETLIKNIRQDEVKLT 297

  Fly   600 -----LKYLNKDATFWTLMAVLTHIATVLITFVTLGVYVWLHRDQEFDLNASRLFSSLALFQQLT 659
                 |:|....|.|::  |:...:|.|:...::.|:            |..|:|::|:.
Zfish   298 RKIGSLRYFYSSAYFFS--AIFVIVAAVVPHALSRGI------------NLRRIFTTLSY----- 343

  Fly   660 VPLLIFPITVPIIIAARVSTRRLERFLKSSEIQKQFEGIRNMARI---LSKSDASLDMYETQEKS 721
              .::..:||         ||:|     ...||..::.:|.:.:|   |||.:..|..|:
Zfish   344 --CMVLRMTV---------TRQL-----PGSIQMWYDTMRLIWKIEEFLSKEEYKLMEYD----- 387

  Fly   722 NMTMRTAQAENRLNEKRLAQKSQTPELATNSTPLLQNAEESAEDISPSTVQELGHNKLVQQRREL 786
                                              |...|...:|::.|.
Zfish   388 ----------------------------------LSITELELQDVTASW---------------- 402

  Fly   787 LRNTPYVAIRPPKMRGSVMERPVEFSVIRARNTDSWRRDSLLLKMPDDIAVSINDGLFTWQPQSQ 851
                       .:..|.::||      |:..|..:...:.             :.|||
Zfish   403 -----------DEGPGELLER------IKQENKANGHHNG-------------DAGLF------- 430

  Fly   852 MPVVQLHVPGII------VPKGKLTIVVGKNGSGKTSLLSALLMEMPLLAGNMFWHKTCTISYVS 910
              ...|:|..::      :.||::..|.|..||||:|||..:|.|:...:|.:  ..:..|||.|
Zfish   431 --FTNLYVAPVLKDISLKLKKGEMLAVTGSMGSGKSSLLMTILGELVPSSGKI--RHSGRISYSS 491

  Fly   911 QQPWLLNDTIRENILFGESFRPKRYDFVLEACALKPDIELMPRGDLSIIGERGINISGGQRQRIA 975
            |..|::..|||:|||||.::...||..|::||.|:.|:..:|..|.:.:.|.|:|:||||:.|:|
Zfish   492 QTAWIMPGTIRDNILFGLTYDEYRYKSVVKACQLEEDLAALPEKDKTPMAEGGLNLSGGQKARVA 556

  Fly   976 IARAIYSSANVVIMDDPLASLDNEVGEHIFQHCIREMLQKSNRTFILVTQQLHRIKEAEYLIAIK 1040
            :|||:|..|::.::|.|...||....:.||..|:.:::  :::|.||||.::..:|.|:.::.:.
Zfish   557 LARAVYRDADLYLLDAPFTHLDIATEKEIFDKCLCKLM--ASKTRILVTNKIEHLKRADKILLLH 619

  Fly  1041 DGRVEACGSYADIELMQPRITAEWNAIIAMAKAKNDNPSQNPGEKTAGERWKLLKNVSKLGLQRS 1105
            :|.....|::.:::..:|    ::::::                               |||
Zfish   620 NGESFFYGTFPELQSERP----DFSSLL-------------------------------LGL--- 646

  Fly  1106 ISVTMDANVACHADAIDGSGCISVANMQSNVVEEDDQVSVSYPIGNASCGGFNLQRKRSSIYGSR 1170
                         :|.|.   ||.....|.:.|...:|||....|        :|.:||:.   |
Zfish   647 -------------EAYDN---ISAERRCSILTETLHRVSVDESAG--------MQPERSAF---R 684

  Fly  1171 HLMYDVPLPIDECQGDDVIMRPRRRHTLGRRGSRNTNSSHRLSGLSTLTATSESSSISGDVLSRS 1235
            .:....|:.|||.:. .||:.|                         |....::|.|.
Zfish   685 QVPPTKPMYIDERKA-SVIVNP-------------------------LGVARKASFIQ------- 716

  Fly  1236 VLATSCSSYAESSVDGGDLATAAPEPRVQSWQPPQHVTHHQPLSRNASSPPAMEVANPDVKKSEE 1300
                                  .||..|:...|          .|..|..|..|:.:.....|:.
Zfish   717 ----------------------VPEEEVRRTLP----------DRKFSLVPENELVDESFMGSDV 749

  Fly  1301 ARRSNTSSESPLDDHVRGSFQQFLRRMS----MRRSNKPKNHHHPLSATNSILSISEESPPVVHF 1361
            ......        |:.|.     ||.|    |..:.......|..|:....||:         .
Zfish   750 YHNHGV--------HMAGQ-----RRQSVLAFMTNAQGQGRREHLQSSFRRRLSV---------V 792

  Fly  1362 PASILATDGNKNETQSEEKPKKCVNIDSKETTINCDDNCYSASDKELRANVTSSPADQEQHNERH 1426
            |.|.||::               ::|.::..:    |:.|..:......|:.:...|:....|
Zfish   793 PQSELASE---------------LDIYTRRLS----DSTYDMTGILEEENIEACLTDEIDEIE-- 836

  Fly  1427 VLAEVAGESGRESMPLARLAIDTERKYGKISDDIYLMYIRAAGLPIITIFFITALIWQCLRVYTD 1491
                       |:....:.  :|..:|  :|::..|:|:      :|.|.||.|:  :.......
Zfish   837 -----------ETFETTKW--NTYVRY--VSNNKSLLYV------LIFILFIAAI--EIAGSVAG 878

  Fly  1492 IWL---QQWSNVHGRVASKGHVVLHPSEQDHEVTY---------YFRMYAAISCVCIIMALVSTP 1544
            |:|   :.|...|.|  |:.::..|.:......||         |:.:|..::....::|:
Zfish   879 IFLITDELWREEHQR--SEPNMTKHSNASSSGQTYAITVTPTSSYYILYIYVATSESLLAM---- 937

  Fly  1545 AGQYAG-------CNARRNLHDKLLQTILHKTLHFFQVTPLGRIVNRFSNDMAVIDKKIAATGQR 1602
             |.:.|       ....:.||.|:|..:|...:........|||:|||:.|||.||..:......
Zfish   938 -GFFRGLPFVHTTITISKKLHQKMLHAVLSAPMSVLNTMKTGRIMNRFTKDMATIDDMLPLLMFD 1001

  Fly  1603 LLQFTLLCLSAILINVTITPWILVLTLPICGAYYLIQKFYRCSARELQRIENATNSPVISHLSET 1667
            .:|.|::.:..||:...:.|:|.:...|:...:.:::|::..:.::|:::|....||:.|||..:
Zfish  1002 FVQLTVVVVGCILVVSIVRPYIFLAATPLAIIFIVMRKYFLRTGQQLKQLETEARSPIFSHLIMS 1066

  Fly  1668 IQGVTTIRAFNQQTRFTEILFKRLEANTIAYALLNTSHRWLGVSLDYLGGCIVFVATVTALTAAS 1732
            ::|:.|||||.:|..|..:..|.|..:|..:.|..::.||.....|.|   .||..|:.|..|..
Zfish  1067 LKGLWTIRAFERQAYFEALFHKTLNTHTATWFLYLSTLRWFLFRADIL---FVFFFTLAAWIAVG 1128

  Fly  1733 VSCRRHYEATTSPSASASPSPFETYAVTKSPSELRPSPSLVGLAINYTLLVPIYLNWVVKLLADM 1797
            .:                                :..|..:|:.|...:|:.....|.|.....:
Zfish  1129 TN--------------------------------QDKPGEIGIIICLAMLILGTFQWCVATSIAV 1161

  Fly  1798 EMYAGSVERIAHYAQGQDADADADADADADVDADLDHEPSSNEDVSAEVDRSSQSDAGDKVYPGA 1862
            :....||:|:..:                                   :|..|::...||
Zfish  1162 DGMMRSVDRVFKF-----------------------------------IDLPSETPKPDK----- 1186

  Fly  1863 TTAAGDVDEDGDQQRIGGARGGGGDCGYRQGHENGAEANADKLNAGNVTGDGNHLNFHHPPATAG 1927
                                                                             
Zfish  1187 ----------------------------------------------------------------- 1186

  Fly  1928 DKVEQATTKTSVIKDKQLPPQQDDKDKKVVLPNEPARKLERYQSVPISWPQRGDIHFDNVSLRYE 1992
                                   .||..:::.|..|:       ...|||.||.|...|::::|.
Zfish  1187 -----------------------GKDSDLIIENVDAQ-------ADSSWPHRGQIEVRNLTVKYT 1221

  Fly  1993 GQKQNVISNLTLKIPAGQRIGICGRTGSGKSSLGLSLFGVLQTTRGHIYIDDVDIQRIRPDELRT 2057
            .....|:.||:......||:||.||||||||||..:|..::.|. |.|.||.|:..::...:.|.
Zfish  1222 EAGHAVLKNLSFSAEGRQRVGILGRTGSGKSSLFNALLKLVYTD-GEISIDGVNWNKMPLQKWRK 1285

  Fly  2058 RLSIIPQDVHLFNATIRENLDPHGYFQDLQLWNCLELAQLKEFVNGHLPLGLDTVICDGGLNLSA 2122
            ...::||.|.:|...:|.||||:|...|.:||...|...||..:. ..|..||..:..||..||.
Zfish  1286 AFGVVPQKVFIFTGPLRMNLDPYGCHSDEELWRVAEEVGLKTVIE-QFPDKLDFQLEYGGYVLSN 1349

  Fly  2123 GHRQLLCLARAILRGSVCLVLDEATSVLDSSTESALLKAADLAFRGRTIITIAHRLTTILDYDRL 2187
            ||:||:||||:||.|:..|:|||.::.||..|...|.|....:|...||:...|::..:|:....
Zfish  1350 GHKQLICLARSILSGARILLLDEPSAHLDPVTIKVLKKTLRQSFSTCTILLSEHKVEPLLECQSF 1414

  Fly  2188 IVLDQGRI 2195
            :::|:|::
Zfish  1415 LMMDKGQV 1422

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Sur	NP_001334732.1	ABC_6TM_SUR1_D1_like	301..682	CDD:350035	73/394 (19%)
		TM helix 1	301..324	CDD:350035	5/22 (23%)
		TM helix 2	385..433	CDD:350035	14/47 (30%)
		TM helix 3	480..525	CDD:350035	4/44 (9%)
		TM helix 4	526..575	CDD:350035	11/50 (22%)
		TM helix 5	587..631	CDD:350035	10/52 (19%)
		TM helix 6	648..681	CDD:350035	5/32 (16%)
		ABCC_MRP_domain1	837..1043	CDD:213217	70/211 (33%)
		ABC_6TM_SUR1_D2_like	1475..1811	CDD:350046	79/354 (22%)
		TM helix 1	1475..1496	CDD:350046	6/23 (26%)
		TM helix 2	1520..1568	CDD:350046	12/63 (19%)
		TM helix 3	1575..1620	CDD:350046	15/44 (34%)
		TM helix 4	1621..1670	CDD:350046	11/48 (23%)
		TM helix 5	1682..1727	CDD:350046	12/44 (27%)
		TM helix 6	1777..1810	CDD:350046	7/32 (22%)
		ABCC_MRP_domain2	1980..2201	CDD:213211	79/216 (37%)
cftr	NP_001038348.1	CFTR_protein	1..1485	CDD:273530	421/2023 (21%)
		Disordered R region. /evidence=ECO:0000250|UniProtKB:P13569	653..826		48/289 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1452..1485
		PDZ-binding. /evidence=ECO:0000250|UniProtKB:P13569	1483..1485