Protein Alignment Col4a1 and col4a1

DIOPT Version :10

• Sequence 1: NP_723044.1
• Gene: Col4a1 / 33727
• FlyBaseID: FBgn0000299
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: XP_002933064.3
• Gene: col4a1 / 100124870
• XenbaseID: XB-GENE-6451485
• Length: 1669
• Species: Xenopus tropicalis
• Alignment Length: 1807
• Identity: 803/1807 - (44%)
• Similarity: 974/1807 - (53%)
• Gaps: 259/1807 - (14%)

  Fly    74 TAGYAGC-VPKC-----IAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGD 132
            :|..:|| ..||     ..:||.||||      ||.|.|||||::||      :|.|||.||:||
 Frog    21 SAACSGCGTSKCDCSGVKGQKGERGLP------GLPGLMGFPGLQGP------EGPPGPLGQKGD 73

  Fly   133 KGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLS---------GMPGPRG 188
            .||.|.||..|..|.||..|..||||.|||.|:||..|..||||..|..         |:||..|
 Frog    74 TGEPGLPGTKGTRGPPGSHGFPGNPGLPGIPGQDGPPGPSGIPGCNGTKGDRGPLGPPGLPGIIG 138

  Fly   189 YAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEH 253
            |.|..|..|.||||....|......|||.|:.|..|:.|..|.||..|..|..||.|:.||.|.|
 Frog   139 YPGSPGQPGPKGEPGDITGIDVSMIKGEKGFPGINGIPGTPGLPGTPGPMGPIGPPGSAGPPGSH 203

  Fly   254 GLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKG-EP-G 316
                            ||||.|||.|   .||        .||:|:.|.:|.||...:.. || |
 Frog   204 ----------------GAPGPKGEMG---ISF--------YGPKGERGDRGPPGAPCKPSTEPQG 241

  Fly   317 PEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGAT 381
            .|.:....|:|||||:||..|:||:.|.       ..||::||||..|..|.||:.|:.|..|..
 Frog   242 SENEQVYYGEKGEKGMPGENGERGQPGQ-------SFKGEKGEPGPLGPRGKPGKDGDQGEKGLQ 299

  Fly   382 GKPGLLGPPGPP--GGGRGTPGPPGPKGPRGYVGAPG-----PQGLNGVDGLPGPQGYNGQKGGA 439
            |.||:.|.||||  ||.:|..|..||:||.|.|.:.|     .:|..|:.|.||.:|..|.||..
 Frog   300 GVPGIPGFPGPPGFGGTKGEKGESGPQGPPGIVVSNGEIEVCEKGDRGLPGFPGSKGDKGLKGAQ 364

  Fly   440 GLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIP 504
            |.||.||:.|.|             ..||.||   ||.||..|:||..||||..:||.||..|.|
 Frog   365 GFPGIPGSPGVP-------------LPGSTGP---PGFPGERGEKGQHGLPGLALQGPKGRDGFP 413

  Fly   505 GYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGA-----PGQKGDAGRPGTPGQ---KGDMG 561
            |.|||                       ||.|||...     .||.||.|.||.|||   :|:.|
 Frog   414 GPPGL-----------------------PGPPGAIDTSIECKSGQPGDPGLPGNPGQNGFQGERG 455

  Fly   562 IKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGER------GYPGERGHDGINGQTGPP 620
            .|||.|..|.||...|         ||:||..|.:||||::      |..|:|||.|.:|..|.|
 Frog   456 QKGDKGDACPSCDLVP---------PGLPGPPGPQGPPGDQGASGVPGIKGDRGHPGYDGPPGAP 511

  Fly   621 GEKGEDGRTGLPGATGEPGKPALCDLSLIEP-LKGDK------GYPGAPGAKGVQGFKGAEGLPG 678
            |.:|..|..|.|||.||||:      ...|| |||||      |:|||||.:|..|..|.:||||
 Frog   512 GPQGSAGLMGSPGAKGEPGE------VFFEPRLKGDKGESGLPGHPGAPGREGRPGIDGQQGLPG 570

  Fly   679 IPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSG 743
            ..|..|..|.|||:||   ||:.|:||..|..|:||..|....|.||..|..|..|..|..|..|
 Frog   571 SKGVAGTIGAKGEQGL---PGDRGSPGNPGERGFPGPAGTGTSGLPGEKGEPGVPGFVGIPGTPG 632

  Fly   744 EKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGL 808
            .|||||...   ||....|..|..|..|.||..||.||||..|..|.:|..|..|.|||    |.
 Frog   633 AKGEPGKAI---IKTGPPGPMGGAGIPGFPGDQGEKGSPGHPGRPGSQGEKGEPGLPGV----GY 690

  Fly   809 NGPRGEKGNQGAVGVPGN---PGKDGLRGIPGRNGQPGPRGEPGISRPGP---MGPPGLNGLQGE 867
            .||.||||.||..|.||.   ||..|:.|:||..|.|||:||||:..|||   .||||..|:.|:
 Frog   691 PGPSGEKGFQGMPGTPGQAGFPGPQGVDGLPGLRGDPGPKGEPGLGLPGPKGVQGPPGPVGIPGD 755

  Fly   868 KGDRGPTGPIGFPGADGSVGYPGDRGDAGL---PGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPG 929
            ||:.|.:|..|..|..|..|:||.:|:||:   ||:.|.||:.|    :||.|..|.:||.|:.|
 Frog   756 KGNPGASGVPGEQGFSGVPGHPGHKGEAGIVGVPGIQGPPGVPG----MGPKGQQGSSGPQGLSG 816

  Fly   930 IDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDK 994
            ..|..|..|.:|..|.||| .|||.|||:|..|:.||.|..|:.|.||..|..|:||..|.||:.
 Frog   817 FPGPAGVKGERGYAGFPGL-DMPGPKGDKGNIGSPGPTGATGIPGQPGAPGKEGLPGFPGPKGEM 880

  Fly   995 GATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPG---- 1055
            |..|:.|:   :|..|.||:||.:|.|||.|..|.||..|..|..||||.:   ||.|.||
 Frog   881 GVMGMPGS---IGQPGAPGSPGALGEKGDAGPFGPPGTSGYPGTKGEKGEE---GLRGEPGKMSE 939

  Fly  1056 -----LPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGI 1115
                 :.|...|||::||.|.||.:|..|..|:||.||:.|..|  |.|.:||.||||.|   ||
 Frog   940 TDKETMKGSKGEKGERGEIGISGEKGSLGDFGSPGMPGKDGQQG--VQGESGPKGEKGSQ---GI 999

  Fly  1116 DGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPG 1180
            .|..|:.|:||..|..|:.|..||||..|..|.||:||:.|:.|..|..|.||.||       .|
 Frog  1000 KGSQGLQGQKGSIGEMGLPGPTGEKGQHGISGSPGSPGLGGIKGEKGLKGEVGLPG-------IG 1057

  Fly  1181 LSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGE 1245
            :.|:||:||:.|..|..|..|..|.||:.||.|.||.|.: ....|..|..|..|..||||.:|.
 Frog  1058 IPGVPGVKGDAGRDGPHGLPGERGDKGDVGIPGMPGFPGS-KGATGHAGIPGSPGLPGEKGAKGT 1121

  Fly  1246 RGLTGPAGVAGAKGDRGLQGPPGASGLNGIP---GAKGDIGPRGEIGYPGVTIKGEKGLPGRPGR 1307
            .||.|..|..|..||.|:|||||.||..|.|   |..|..|.:||.|.||      :||||.||.
 Frog  1122 HGLVGIPGQKGLTGDSGVQGPPGHSGEKGEPGYDGIPGSPGEKGEAGIPG------RGLPGHPGE 1180

  Fly  1308 NGRQGL---IGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGL-----AGSPGQPGQDGF 1364
            :|.:|.   ||.||..|..|:||:.|:.||.|..||.||.|..|..|:     .|..|||||.|:
 Frog  1181 HGEKGSKGDIGFPGAPGISGVPGIKGDQGLPGFQGPQGPPGFPGPSGIPMEGPKGEKGQPGQAGY 1245

  Fly  1365 PGAP---GLKGDTGPQGFKGERGLN------GFEGQKGDKGDRGLQGPSGLPGLVGQKGDT---- 1416
            ||:|   ||.|.||..|.|||:|..      ||:|.|||.|::|..|.:|..|:.||||||
 Frog  1246 PGSPGPVGLPGHTGQDGLKGEKGERGWPGALGFQGIKGDTGNQGPSGDAGPSGITGQKGDTGPPG 1310

  Fly  1417 --GYPGLNGN---DGPVGAPGERGFTGPKGRDGRDGTPG----LPGQKGEPGMLPPPGPKGEPGQ 1472
              |:|||.|:   .|..|..|::|.|||||..|..|..|    ..|:.||||...|.||:|..|.
 Frog  1311 IPGFPGLKGSLGISGEKGDRGDQGITGPKGDLGPPGPAGTHTVTKGESGEPGPQGPSGPRGIQGL 1375

  Fly  1473 PGRNGPK---GEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGAIG 1534
            ||..|.|   |..|.||..|:.||.|..|:|||.|..|.||..|.|||      ||..|..|.||
 Frog  1376 PGPKGDKGVTGASGLPGPSGIPGIHGTPGQKGETGPSGPTGQTGYPGP------PGSDGLPGPIG 1434

  Fly  1535 LIGQKGEPGAPAPAALDYLTGILITRHSQSETVPACSAGHTELWTGYSLLYVDGNDYAHNQDLGS 1599
            |      ||:|:.|     .|.|:|||||:...|.|..|.|.:::|||||||.||:.||.||||:
 Frog  1435 L------PGSPSMA-----HGFLVTRHSQTTETPQCPFGTTAVYSGYSLLYVQGNERAHGQDLGA 1488

  Fly  1600 PGSCVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAAIP--MMPVENIEIRQYISRCVVCEAP 1662
            .|||:.:|||:|.|.|..|||||:|||||.::||:|...:|  |.|:....|:.:||||.|||.|
 Frog  1489 SGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPITGDGIKPFISRCTVCEVP 1553

  Fly  1663 ANVIAVHSQTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGGQALQSPGSCLEDFRATPFIECNGA 1727
            |.|:|||||||::|.||:||..|||||||:|.|:.|..|.||||.|||||||:||:.|||||:|
 Frog  1554 AMVMAVHSQTIQIPQCPDGWISLWIGYSFVMQTSAGAEGSGQALASPGSCLEEFRSVPFIECHG- 1617

  Fly  1728 KGTCHFYETMTSFWMYNLESSQPFERPQQQTIKAGERQSHVSRCQVCMKNSS 1779
            :|||::|....|||:..:|.::.|::|...|:|||:.::|||||||||:.:|
 Frog  1618 RGTCNYYANTYSFWLATIEKNEMFKKPTPSTLKAGDLRTHVSRCQVCMRRTS 1669

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	gly_rich_SclB	<107..>361	CDD:468478	109/264 (41%)
		gly_rich_SclB	<355..>642	CDD:468478	126/307 (41%)
		gly_rich_SclB	<543..820	CDD:468478	131/292 (45%)
		gly_rich_SclB	<727..>968	CDD:468478	113/249 (45%)
		gly_rich_SclB	<969..>1218	CDD:468478	112/257 (44%)
		gly_rich_SclB	<1186..>1420	CDD:468478	115/259 (44%)
		gly_rich_SclB	<1321..>1547	CDD:468478	113/255 (44%)
		C4	1555..1662	CDD:128421	59/108 (55%)
		C4	1663..1777	CDD:128421	67/113 (59%)
col4a1	XP_002933064.3	gly_rich_SclB	<39..>297	CDD:468478	127/303 (42%)
		gly_rich_SclB	<268..>463	CDD:468478	95/240 (40%)
		gly_rich_SclB	<487..>728	CDD:468478	113/256 (44%)
		gly_rich_SclB	<778..>1020	CDD:468478	112/257 (44%)
		gly_rich_SclB	<902..>1164	CDD:468478	122/277 (44%)
		gly_rich_SclB	<1179..>1431	CDD:468478	113/257 (44%)
		C4	1445..1551	CDD:460201	56/105 (53%)
		C4	1555..1666	CDD:460201	65/111 (59%)