Protein Alignment Mad and smad4a

DIOPT Version :10

• Sequence 1: NP_001259992.1
• Gene: Mad / 33529
• FlyBaseID: FBgn0011648
• Length: 525
• Species: Drosophila melanogaster
• Sequence 2: XP_017213465.1
• Gene: smad4a / 559111
• ZFINID: ZDB-GENE-990603-8
• Length: 548
• Species: Danio rerio
• Alignment Length: 532
• Identity: 192/532 - (36%)
• Similarity: 263/532 - (49%)
• Gaps: 124/532 - (23%)

  Fly    98 VKKLLGWKQGDEEEKWAEKAVDSLVKKLKKRKGAIEELERALSCPG-QPSKCVTIPRSLDGRLQV 161
            |..|:..:||.|.|.:|::|::|||||||::|..::.|..|::..| .|||||||.|:|||||||
Zfish    17 VHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQV 81

  Fly   162 SHRKGLPHVIYCRVWRWPDLQSHHELKPLELCQYPFSAKQKEVCINPYHYKRVESPVL------- 219
            :.|||.|||||.|:||||||.. :|||.::.||:.|..|...||:|||||:||.||.:
Zfish    82 AGRKGFPHVIYARLWRWPDLHK-NELKHVKYCQFAFDLKCDSVCVNPYHYERVVSPGIDLSGLTL 145

  Fly   220 ------------------PPVLVPRHS-----EFAPGHSMLQFNHVAEP-SMPHNVSYSNSGFNS 260
                              ||.|.....     :..|..::.|     || |.|..:..:....::
Zfish   146 SGSGPSGLMVKDEYDYDGPPSLPSTEGHMQTIQHPPSRAVTQ-----EPFSTPALLPPAEGSSSA 205

  Fly   261 HSLSTSNTSVGS---PSSVNSNPNSPYDSL----AGTP--------PPAYSPSEDGN-----SNN 305
            .|.:.|:.:|||   |.||.|..:|. |||    :||.        ||..:.:...|     |.|
Zfish   206 SSSAFSSIAVGSTTQPGSVLSGSHSS-DSLLQIASGTGQGSQQNGFPPGQTSTYHHNPTSSWSRN 269

  Fly   306 PNDGGQLLDAQMGDVA----------------QVSY--------SEPAFWASIAYYELNCRVGEV 346
            .|....:...|.|.:.                ::::        :.|.:|.||||:|::.:|||.
Zfish   270 SNFTPSVPHHQNGHLQHHPPMAHPAHYWPVHNEIAFQPPISNHPAAPEYWCSIAYFEMDVQVGET 334

  Fly   347 FHCNNNS--VIVDGFTNPSNNSDRCCLGQLSNVNRNSTIENTRRHIGKGVHLYYV-TGEVYAECL 408
            |...::.  |.|||:.:|| ..||.||||||||:|...||..|.||||||.|... .|:|:..||
Zfish   335 FKVPSSCPIVTVDGYVDPS-GGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCL 398

  Fly   409 SDSAIFVQSRNCNYHHGFHP-STVCKIPPGCSLKIFNNQE------------------------- 447
            ||.|:||||...:...|..| ..|.||.|...:|:|:.::
Zfish   399 SDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAG 463

  Fly   448 ----------FAQLLSQSVNNGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHG 502
                      .|..:|.|...|. .|.:|.::|.:|||||||||.:|.||.:..||||||||||.
Zfish   464 NIPGPGSVGGIAPAISLSAAAGI-GVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHR 527

  Fly   503 PLQWLDKVLTQM 514
            .||.||:||..|
Zfish   528 ALQLLDEVLHTM 539

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mad	NP_001259992.1	MH1_SMAD_1_5_9	93..216	CDD:199814	65/118 (55%)
		MH2_SMAD_1_5_9	325..525	CDD:199822	93/237 (39%)
smad4a	XP_017213465.1	MH1_SMAD_4	11..135	CDD:199816	65/118 (55%)
		PAT1	<164..>314	CDD:401645	32/155 (21%)
		MH2_SMAD_4	316..537	CDD:199823	91/222 (41%)