back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Obsc and spega

DIOPT Version :10

Sequence 1:	NP_001097440.1	Gene:	Obsc / 3346201	FlyBaseID:	FBgn0053519	Length:	4218	Species:	Drosophila melanogaster
Sequence 2:	NP_001007110.2	Gene:	spega / 570504	ZFINID:	ZDB-GENE-030131-3230	Length:	2995	Species:	Danio rerio

Alignment Length:	3456	Identity:	701/3456 - (20%)
Similarity:	1116/3456 - (32%)	Gaps:	1298/3456 - (37%)

- Green bases have known domain annotations that are detailed below.

  Fly  1579 KATNE--HGSATSSTQL---LIKCAPEFTHKLKNITVAEGDSNVELVVGVDAYPRPHAKWYIDGI 1638
            |.|.|  |.|:.:|:.:   .|...|.|..|||...||.| .:|.|.|.|...|||...||
Zfish     3 KVTEEKRHSSSMNSSTVETSFIAAPPVFLRKLKWAAVAAG-CDVRLRVSVGGNPRPTLHWY---- 62

  Fly  1639 EIDEKRNDFRHVEEGNDFKLIMNQVATNMQGNYTCKIMNDYGKLEDNCVVTVNCKPKVKRGLKNV 1703
                      |    ||..|:::
Zfish    63 ----------H----NDDPLVID------------------------------------------ 71

  Fly  1704 EVQEGKSFTLEVEVYSEPEAKIKWFKDGHEIYEDARIKISRDTQRIENYYLTLNLARTEDAGTYE 1768
                                        ||.|:...|   ||.|:             .|.|.|.
Zfish    72 ----------------------------HEDYDGLWI---RDCQQ-------------ADGGLYT 92

  Fly  1769 MKATNFIGETTSTCKVAVLTSEALSLEQTVTKTLIATTEEPEEGAVPEIVHVDVFQQHSYESVPL 1833
            ..|.|.:||..::..:||     |.||:....|       .:|.|.|::            |:.:
Zfish    93 CVAVNHLGEARTSAVLAV-----LDLEEDSNST-------EDESAEPQV------------SMEM 133

  Fly  1834 KYEVIATGIPKPEAIWYHDGKPITPDKHTAITVDGDHYKLEVQSLDLVDAGEYKVVVQNKVGEKS 1898
            |.:.:.   |:.|||   :.:|         |..|......:.|..||...|.:.     :|.::
Zfish   134 KEQFMP---PQGEAI---NSQP---------TGRGRAMLSHIPSDGLVVEREMRA-----LGSRA 178

  Fly  1899 HQGELSLSGIAEYRKPILTQGPGLKDIKVNKGDKVCEPVVFTADP--APEIVLLKDGQPVVETNN 1961
                   .|:.|...|    |.|..|.|.::    ..|.|.|..|  |...:...|....:::..
Zfish   179 -------PGLQEPLSP----GRGQLDFKTSE----ASPFVQTQPPHKAQASITKSDVDATIDSTA 228

  Fly  1962 VKLKVDKKDAENGLVQYTCTLNILEAEIKDSGRYELKVKNKYGELVTSGWID--VLAKPEISGLN 2024
            .|:| ..|.|.||          .|..||.|     |:.   |...:.|..|  .:..|::|..:
Zfish   229 TKIK-GTKTAMNG----------AEVSIKSS-----KIT---GSHQSGGSQDPSSIQTPKVSQAS 274

  Fly  2025 DTKCLPGDTI-CFEALVQANPKPKV----SWTRGNENLCNHENCEVIADVDADKYRLVFQSVSPC 2084
             :|.|  |.: .||.....:..|||    ||.....:.||.|:                   ..|
Zfish   275 -SKIL--DRVRAFEEQSHNSNMPKVSSRLSWGFNRTSSCNSED-------------------ETC 317

  Fly  2085 EDGKYTITATNSEGRAAVDFNL----AVLVEKPTFIVQPESQS-----IHDYRPVSTKVLVHGVP 2140
            :.||:   ..|::...|:..:.    |..:|:.:..||...||     :|..:
Zfish   318 KAGKF---QANTKSDVALKRSFFKQKASSLEEQSTCVQKNFQSKLSEELHRIK------------ 367

  Fly  2141 LPTIEWFKDDKPINYEAINKPGKDKLYAKED----TKKGTDQIESVLDIKSFRENDVGAYTCVAT 2201
                           :.:.|....|.::.|.    .|:.:...||||.....:..:.|.:   .|
Zfish   368 ---------------KLVGKSNIKKAFSMEQLTQTDKQSSVSTESVLTQVIQKTEETGKH---FT 414

  Fly  2202 NEIGVTKAPFKLAMLSLAPSFVKKLDNALDVLQGEPLVLECCVDGSPLPTVQWLKDGDEVKPSES 2266
            |...|..|..:...|....|                         |.||.:..   .|:.|..|:
Zfish   415 NLKAVPDAKERWTTLPKEQS-------------------------SRLPKINL---ADKTKQPEN 451

  Fly  2267 IKISTNPDGLVKLEINSCQPNDSGAYKLIISNPHGEKVALCAVAVKPEEMQPKFLKPITSQTVVV 2331
               .|.|      |:|..|.|:|...:|:......|||:..          |....|:..:|.|.
Zfish   452 ---ETPP------EMNENQENNSKPIQLLDGQVLNEKVSFI----------PGQCSPMLPRTNVS 497

  Fly  2332 GEPLKLEAQVTGFPAPEVKWYKDGMLLRPS--PEINFINSPNGQIGLIIDAAQPLDAGVYKCLIA 2394
            .:..|..||      |.|   |||::..|.  |.:
Zfish   498 RKWPKSPAQ------PMV---KDGLVKAPQKPPRL------------------------------ 523

  Fly  2395 NKGGEIEGVSKVEIVPKESKPVFVAELQDASSIEGFPVKMDI-----KVVGNPKPKLQWFHNGHE 2454
                 :|.:|.:....|.:.|..|        :|..||..::     :::...:..|...|...|
Zfish   524 -----LESISTLPTPFKMTIPTIV--------VENKPVDEELDQKEGQIMRQNRDALDDCHTSVE 575

  Fly  2455 IKPDASHIAIVENPDNSSSLIIEKTAPGDSG---LYEVIAQNPEGSTASKAKLYVAPKADETATE 2516
                   .:|.|.|   .|.:..|.|.|.:|   |..:|.:|......:::.|.:
Zfish   576 -------KSIAEAP---MSELPRKDALGTAGSELLQCIIKENTVARAPAESLLII---------- 620

  Fly  2517 EAPQFVSALRDVNADEGQELVLSAPFISNPMPEVIWSKDGVTLTPNERLLMTC----DGKHIGLT 2577
                 ...::||....|:..:|......:...:|.|..:|..:.|   .|..|    ||....|.
Zfish   621 -----TRPMQDVKVKAGETALLECFIAGSQAVDVDWLANGKLIQP---ALFDCKMQFDGHRCRLL 677

  Fly  2578 IKPAEAADSGNYTCLLANPLGEDSSACNANVRKV-YKPPVFTQKISDQQQVFGNNAKIPVTVSGV 2641
            .|.|...|||.|||.|:.  .::...|.||:..: .|.|:||:|:...:.:.|.:|:....|||.
Zfish   678 FKSAHENDSGCYTCKLST--AKEELICTANLLVIPSKEPLFTRKLEVLEAIEGRSAQFDCKVSGC 740

  Fly  2642 PYPDLEWYFQDKPIPKSEKYSIKNDGDHHMLIVNNCEKGDQGVYKCIASNREGKDITQGRLDIVN 2706
            |.|::.|...:||:.:|:...|.|...||.|::.:..|..:|:|..||.|..||..:...| .|.
Zfish   741 PPPEVTWTHCEKPLVESDNIHILNVNGHHSLLITHVNKESEGLYTAIAQNVHGKAASSAEL-YVQ 804

  Fly  2707 E----IKKH-SR--------SEP------------PVFLKKIGDCDIYEGMVAKFTACATGYPEP 2746
            |    |..| ||        .||            |.|:|.:.|.::.||..|......||.|.|
Zfish   805 EPRPAISTHMSRLEKMPSIPEEPEVPEGEVERRTMPDFIKPLSDLEVIEGKEAVLKCRVTGLPYP 869

  Fly  2747 EVEWFKNDQKL-FPSDRFLI---DIEPNGLLRLTIKNVTEYDVGRYSCRIFNPYGDDICHAELFY 2807
            ::.|:.|.:|: ..:||.:.   |:.     .|.|::|.....|.|.|.|.|..|...|:|.|
Zfish   870 KITWYHNGKKIESTNDRKMTQYRDVH-----SLVIQSVCHDHSGVYKCVISNKVGKAACYAHL-- 927

  Fly  2808 DSLDSQQKPLEDQYTDFKKYKKSGAPPPLSEGPIISRMTDRGLLLSWNPSVPLTPRY---PITYQ 2869
                               |.....|.|....|:|..:|.|.:||||.....|.|..   .:.|.
Zfish   928 -------------------YVAVSLPEPPDGPPVIESVTGRMILLSWKKPKNLDPSIDPASLMYV 973

  Fly  2870 IEMMDLPEGDWRTLRTGVRSCACDIRNLEPFRDYRFRVRVENKFGVSDPSPYTQTYRQKLVPDPP 2934
            ::...|....|.|:.:.:...:..:.:|.....|.|||.......:|.||..|...  :||
Zfish   974 VQQQVLGSTQWTTIASSLTDTSYTVTSLSKGVCYSFRVLSTTGKTLSKPSQPTDLV--QLV---- 1032

  Fly  2935 KTYTYLPPGTDFRPETSPYFPKDFDIERPPHDGLAQAPQFLLREQDISYGVKDHNTEL------- 2992
                          :...||.|              || .::.:.||.|.|::....:
Zfish  1033 --------------DRGEYFRK--------------AP-VIIDKPDIVYAVENQPVTITITINHV 1068

  Fly  2993 ----MWFVYGYPKPKMTYYFDDMLIESGGRFDQSYTRNGQATLFINKMLDRDVGWYEAVATNEHG 3053
                .|...|.           :|:...|..:.:...:.|..|.|.|:...|||....:|.|::|
Zfish  1069 QATCTWKRRGV-----------VLVNKLGALEMTTPDDDQHALHIAKVKSTDVGQLIFMANNQYG 1122

  Fly  3054 EARQRVRLEIAEHPRFLKRPDETFIMARKNGRIEAKLVGIPLPEVHWFKDWKPIVDSSRIKISSY 3118
            .....::|.||..|.|....::..:...:.......:.|.|.|::.|:||...:.:||.:.. .|
Zfish  1123 SDLCTLQLVIAVPPIFETIMEDLDVCVGETCHFAVVVDGKPDPDILWYKDGVLLAESSHLTF-VY 1186

  Fly  3119 DPDIYVLSIHDSIIKDGGLYSISARNIAGSIS--TSVTVH----IEENEDQYIYKTYGRHPYVRS 3177
            |.....|.:.::..:|.|:|:.:|:|:|||:|  ..:|||    :||.|:|     ......:..
Zfish  1187 DDRECSLVVLNAQPEDVGVYTCTAKNLAGSVSCKAELTVHTAQNVEEEEEQ-----MEDEATILR 1246

  Fly  3178 KQLRYQDKYDIGDELGRGTQGITY-HAVERSSGDNYAAKIMYGRPELRPFMLNELEMMNTFNHKN 3241
            :.....|.|||..|:|||  ..:| ..|:..:..::|||.:..|.:.:...|.||.::...:||:
Zfish  1247 RMRMLTDYYDIHKEIGRG--AFSYVKRVKHKNDQSFAAKFISVRAKKKTCALRELALLAELDHKS 1309

  Fly  3242 LIRPYDAYDTDRSVTLIMELAAGGELVRDNLLRRDYYTERDIAHYIRQTLWGLEHMHEMGVGHMG 3306
            ::|.:||::..|.|.::.||.  .|.:.:.:.:|....|.::...|||.|.|:|::|:..:.|:.
Zfish  1310 IVRFHDAFEKRRVVIILTELC--HEELLERITKRTTILESEVQSIIRQLLEGIEYLHQNYIIHLD 1372

  Fly  3307 LTIKDLLISVVGGDIIKVSDFGLSRKINRHNLSTLDYGMPEFVSPEVVNKEGVNFSHDMWTVGLI 3371
            |..:::|::....|.|::.|||.:.|:..:......||.|||::||:||:..::.|.|:|.||:|
Zfish  1373 LKPENILMADQKTDQIRICDFGNALKVKPNEELYCKYGTPEFIAPEIVNQSPISKSTDIWPVGVI 1437

  Fly  3372 TYVLLGGHNPFLGIDDRETLTKIREGRWDFKDEIWTHISDDGRDFISRLLLYSPEERMDVKTALK 3436
            ||:.|.|.:||.|.:||:||..||.....|::.::..:..:.:.||.::|: |.:.|.|....|.
Zfish  1438 TYLCLTGVSPFAGENDRDTLLNIRNYNVAFEESMFKDLCREAKGFIIKVLV-SNKLRPDATECLL 1501

  Fly  3437 HPWFFML---------------------------------------------------------- 3443
            ||||..|
Zfish  1502 HPWFKSLTKGKSINTTLHKQVLARRKWQCSLIRYGSKMVMRSISELLDDSSSHVSLAVPRNLKDG 1566

  Fly  3444 ----------------------------------------------------------------- 3443

Zfish  1567 SPPPSSSSDSDEDIDELPFIPMPHTMMFSGSRMSLTEIHEDDDKVIRGSNESYKKNLNQLDDIPE 1631

  Fly  3444 ------------------------------------------------------------DRPVY 3448
                                                                        |..:.
Zfish  1632 SQIIAGQKNEDYLKNPKRTDNCLQRGSSVEVDQVASKTRRGLMRRGSSADSALLLQITPEDNEIK 1696

  Fly  3449 D-------------------------------HDYQIGTDRLR---------------------- 3460
            |                               .||.:..|.:|
Zfish  1697 DTTEDSQKHMKKAVSMELPHRSSSPKTAKLSKEDYALKLDLMRQRLLKGGTVNKNMSGLRGPLLE 1761

  Fly  3461 -----------NYYDHFRDWYANAS---------------CKNYFRRR--------------RLS 3485
                       :...:||:...|||               .|..||:|              |.|
Zfish  1762 TLGVDDERRTSSLDRNFRNARLNASGDSGTFNNDSSEETYQKPAFRKRSSLRDENSESISLHRRS 1826

  Fly  3486 GC-FQHPSKMV------------------------------YPPGHVY----------------- 3502
            |. .:.||...                              .||..|.
Zfish  1827 GAPLEIPSSSTGDHNVLKIKSTILDENKANLPPLFPRDISSKPPTPVLKNKQVSKEEANSDVLIM 1891

  Fly  3503 ------------------------TPEN----------------------------------TPE 3509
                                    .|||                                  ||.
Zfish  1892 HSSRSAFNLEDTEIMVEEMKEQDSVPENMNKSTEFPLDILPHDISSNYCSKLQANGKKASFLTPL 1956

  Fly  3510 PLP--------------------------EPRIRA----------------KREEVVSKYLHPDY 3532
            |.|                          :|.:|:                |||   |...|...
Zfish  1957 PTPVLKISQPNIQPTAGRPGVFASAFSAHQPNLRSDIKNIDSEEIFEARFKKRE---SSLAHGLK 2018

  Fly  3533 ELGLIQSESH------------YQYGP--------DTYLLQ-------LRDVN----------FP 3560
            .|...:||..            |:.||        .|.|.:       ||:|:          |.
Zfish  2019 RLTRTKSEESSPVPQRKSDEVVYRPGPVGSPLEFGSTGLKEKSKSVQDLREVDKEIGLGLIGRFS 2083

  Fly  3561 VRLREYMKV--------------------AHRRSPSFALNDSVDWS-------------LPVIRE 3592
            :|.|:...:                    |.|||.|....::.:.:             .||:..
Zfish  2084 MRARKLQPIDKKEKKEISDSVTNKRQLTWATRRSKSLDKKENFETNKENLEKDTKKIAESPVLAV 2148

  Fly  3593 RRRF----TDIMD------EEIDDERTRSRISMYAAN-------------------------ESY 3622
            ||:|    :.|.|      ::..|:.|:..|...|:|                         ..:
Zfish  2149 RRKFESNVSGIFDRVHSRSKDRKDKETKPHIDAEASNVEKQDMKKINDSPVLALRKKFETKVSGF 2213

  Fly  3623 SIRRL------------------------------------------------------------ 3627
            |:|:.
Zfish  2214 SLRKQSQSEGEGTKFEGQKTPLFSRHHRSQSDGLIHKKMDIPENQLPLQTTTISSKETLNSSSSA 2278

  Fly  3628 ---------RTELGPRLDEY-------------TEADAMIETQREGYPPFFREKPQTIAITENQP 3670
                     .||:..|.|.:             :.|.|   |.:|.:||.|....:...:.|..|
Zfish  2279 HSIESSQTPETEIRSRWDRWGLSRGKRDRTPSISRAPA---TPKEDFPPVFHIALKDHVLLEGNP 2340

  Fly  3671 SHIHCFAVGDPKPCVQWFKNDMVLTESKRIKISVDEDGRSILRFEPALHFDVGVYKVVARNKVGQ 3735
            ..:.|...|.|:|.:.|.|:...|.....:.:....|||.:|........|.|:|:.||.|.:..
Zfish  2341 VTLSCLPAGSPEPKILWLKDKKPLKICDGMNLEACPDGRQLLMIMNISKKDAGIYECVATNNLAS 2405

  Fly  3736 TVARCRIVVATLPDAPDSPEISANSGTEILLRWKQPRDDGHSTVLC-YSLQYKLSNCDAWTTVAD 3799
            ....|.:.:|.:|..|.:|||.......:|::|| |.|   :.|.| |:::.|....|.|.|.|.
Zfish  2406 VTTSCILTLACIPSCPGTPEIRQIYNNTVLVQWK-PSD---TKVPCTYTIEKKFDGDDKWLTEAT 2466

  Fly  3800 NIDHEFYLLHDLQPNTNYQFRLASKNRIGWSEM-----GIPVSASTVGGDAP---KIH------- 3849
            .:...|:...:|...:..:||:|..|:.|.|..     |:.:.........|   |.|
Zfish  2467 GVTDCFFNSSELPSGSTIRFRVACVNKAGQSPYSNESDGVSIDTKVTPQHQPAKMKTHSPAFFPA 2531

  Fly  3850 ITKA-----------------------------------------------------MKHLQQLT 3861
            :|.|                                                     :..:..:|
Zfish  2532 VTAAVATSAFSLSQPSVFSQSISPTPAQSADVSNTFLEVQSSPKMSTPLDLPKPASSVNTMPPIT 2596

  Fly  3862 EN--------------GHQVVPEEERVHTDYHCEREP--PNWVTDSSVS---------------- 3894
            :.              |..:.|....|...|.....|  |:.:..||:|
Zfish  2597 QTQTVSPRSYTAPPSIGRSISPVPTYVPATYSLAPTPVSPSVIVVSSISPIGEEASSPTPETPTG 2661

  Fly  3895 ------------------DKYSFISEIARGEFSTIVKGIQKSTDTVVVAKILEVTDENEDNVVAE 3941
                              ..|||:.|.|||.|..|....:.:|..:.:|||:....:.:..::.|
Zfish  2662 QAVSSIKSETTLRQGVPQKPYSFLDEKARGRFGVIRDCRENATGKMFIAKIIPYDQKTKQTIIKE 2726

  Fly  3942 FDNFKTLRHERIPALFSAYKPLNVPIAIFVMEKLQGADVLTYFSSRHEYSEQMVATVVTQLLDAL 4006
            ::..|:||.|||.||..||  :.....:.:.|...|.::|.....|..|||..|...:.|:|..|
Zfish  2727 YEILKSLRCERIMALHEAY--ITPRYLVLITEYCSGKEILQNLIDRFCYSEDDVVGFIVQILQGL 2789

  Fly  4007 QYLHWRGYCHLNIQPDNVVMASVRSIQVKLVDFGSAKKVNKLGMKVTPC----GSLDFQPPEMIN 4067
            :|||.....||:|:|||:::.::..|  |::|||||::.|.|.::  .|    |:|::..|||:.
Zfish  2790 EYLHNCKILHLDIKPDNIMVTNLNVI--KIIDFGSAQRFNPLSLQ--QCSRYLGTLEYMAPEMLK 2850

  Fly  4068 DEPIFPQSDIWSLGALTYLLLSGCSPFRGADEYETKQNISFVRYRFENLFKEVTPEATRFIMLLF 4132
            .:.:.|.:||||||.|:|::|||..||...|...|:..|...::....|:.:|:..|:.|:..:.
Zfish  2851 GDLVGPPADIWSLGVLSYIMLSGRHPFEDKDPQLTEAKIHEAKFDSTKLYPKVSQSASTFLKKIL 2915

  Fly  4133 KRHPTKRPYTEDCLEHRWLMSSDYMVRKRERAIFLGSRLKTFCDEYHDLKNASATSSKV-LNTVA 4196
            ..:|..||..:|||.|.||..|.....:|:...|..:|||.|..|:......|||..|| |....
Zfish  2916 NSYPWCRPTIKDCLIHSWLHDSYLKKLRRQTLTFTTTRLKEFMGEHQRRCAESATKHKVILRVYQ 2980

  Fly  4197 GGP----TPTQ 4203
            |||    :||:
Zfish  2981 GGPSSPASPTK 2991

Known Domains:

Indicated by green bases in alignment.

Software error:

Illegal division by zero at /www/www.flyrnai.org/docroot/cgi-bin/DRSC_prot_align.pl line 592.

For help, please send mail to the webmaster (ritg@hms.harvard.edu), giving this error message and the time and date of the error.