Protein Alignment Polr1B and RET1

DIOPT Version :10

• Sequence 1: NP_476708.1
• Gene: Polr1B / 33210
• FlyBaseID: FBgn0003278
• Length: 1129
• Species: Drosophila melanogaster
• Sequence 2: NP_014850.1
• Gene: RET1 / 854382
• SGDID: S000005733
• Length: 1149
• Species: Saccharomyces cerevisiae
• Alignment Length: 1207
• Identity: 336/1207 - (27%)
• Similarity: 531/1207 - (43%)
• Gaps: 205/1207 - (16%)

  Fly    19 PEFKQIPKKLSRHLANLGGPHVDSFDEMLTVGLDN--SAKHMIPNHWLSPAGEKISMKVESIWIA 81
            |.|.::...:.:||        |||:..:...|..  .|..:|    ||....:..:|...|.:.
Yeast    49 PAFLKVKGLVKQHL--------DSFNYFVDTDLKKIIKANQLI----LSDVDPEFYLKYVDIRVG 101

  Fly    82 KPKVPQDVIDVRTREIYPTDSRQLHVSYSGMCSVRLGWSVNGVQKTPINMDLGEVPIMLRSKACN 146
            | |......|..|.   |.:.|...::||....|.:.::.........::::|.:||||||..|.
Yeast   102 K-KSSSSTKDYLTP---PHECRLRDMTYSAPIYVDIEYTRGRNIIMHKDVEIGRMPIMLRSNKCI 162

  Fly   147 LGQATPEEMVKHGEHDSEWGGIFVIRGNEKIVRMLIMTRRNHPICVKRSSWKDRGQNFSDLGMLV 211
            |..|...:|.|..|...:.||.|::.|.||::.:.....:|. |.|:....|         |::
Yeast   163 LYDADESKMAKLNECPLDPGGYFIVNGTEKVILVQEQLSKNR-IIVEADEKK---------GIV- 216

  Fly   212 QTVREDESSLSNVVH------YLNNGTAKFMFSHVKRLSYVPVCLILKCLMDYTDEEIYNRLVQG 270
                  ::|:::..|      |:.....|....|......:|:.::||.....:|.||. :||.|
Yeast   217 ------QASVTSSTHERKSKTYVITKNGKIYLKHNSIAEEIPIAIVLKACGILSDLEIM-QLVCG 274

  Fly   271 YESDQYYVSCVQAMLREVQNENVYTHAQCKSFIG----NLFRARFPEVPEWQPDDDVTDFILRE- 330
              :|..|.......|.|....::||..|...:||    .:.|.:..              ||:|
Yeast   275 --NDSSYQDIFAVNLEESSKLDIYTQQQALEYIGAKVKTMRRQKLT--------------ILQEG 323

  Fly   331 ------RVMIHLDT----YEDKFQLIVFMIQKLFQCAQGKYKVENVDSAMMQEVLLPGHLYQKYL 385
                  .|:.||..    :.:|...|..|.:::.........:::.|....:.:.|.|.|.....
Yeast   324 IEAIATTVIAHLTVEALDFREKALYIAMMTRRVVMAMYNPKMIDDRDYVGNKRLELAGQLISLLF 388

  Fly   386 SERVESWVSQVRRCLQKKLTSP------DALVTSAVMTQCMRQAGGVGRAIESFLATGNIASRTG 444
            .:..:.:.:..:..:.|.|..|      |||::..|.:..:  ..|:.|||    :|||.:.:.
Yeast   389 EDLFKKFNNDFKLSIDKVLKKPNRAMEYDALLSINVHSNNI--TSGLNRAI----STGNWSLKR- 446

  Fly   445 LGLMQNSGLVIMAENINRMRYMSHFRAIHR-GSYFTTMRTTEA-RQLLPDAWGFICPVHTPDGTP 507
             ..|:.:|:..:   ::|:.|:|....:.| .|.|...|.... |.|.|..:|.:|...||:|..
Yeast   447 -FKMERAGVTHV---LSRLSYISALGMMTRISSQFEKSRKVSGPRALQPSQFGMLCTADTPEGEA 507

  Fly   508 CGLLNHLTLTCEISMRPDPKLVKAI-------PKHLIDMGMMPLSNRRYLGEKLYVVFLDGKHLG 565
            |||:.:|.|...|:...:.:.:|.:       ...|||...:.|:         |.|:|:|..:|
Yeast   508 CGLVKNLALMTHITTDDEEEPIKKLCYVLGVEDITLIDSASLHLN---------YGVYLNGTLIG 563

  Fly   566 HIHQSEAEKIVDELRYGKIFGTLPQMMEIGFIPFKKNGQFPGLYIATGPARMMRPVW-------- 622
            .|.  ...|.|.:.|:.:..|.:.:     ||....|.....::|||...|:.||:.
Yeast   564 SIR--FPTKFVTQFRHLRRTGKVSE-----FISIYSNSHQMAVHIATDGGRICRPLIIVSDGQSR 621

  Fly   623 ------------------NLKWKRVEYIGTLEQLYMEIAIDAKEMYPDFTTHLELAKTHFMSNLA 669
                              .||...|||:...|:....||:..|::.|.. ||||:.....:..:|
Yeast   622 VKDIHLRKLLDGELDFDDFLKLGLVEYLDVNEENDSYIALYEKDIVPSM-THLEIEPFTILGAVA 685

  Fly   670 NLIPMPDYNQSPRNMYQCQMGKQTMGTPCLNWPKQAANKLYRLQTPGTPLFRPVHYDNIQLDDFA 734
            .|||.|.:||||||.|||.||||.:|....|..|:....||.:..|..|:.:....:.|..|...
Yeast   686 GLIPYPHHNQSPRNTYQCAMGKQAIGAIAYNQFKRIDTLLYLMTYPQQPMVKTKTIELIDYDKLP 750

  Fly   735 MGTNAIVAVISYTGYDMEDAMIINKAAYERGFAYGSIYKTKFLTLDKKSSYFARHP-HMPELI-- 796
            .|.||.|||:||:|||:|||:::||::.:|||.       :..|..|.::...|:. |..::|
Yeast   751 AGQNATVAVMSYSGYDIEDALVLNKSSIDRGFG-------RCETRRKTTTVLKRYANHTQDIIGG 808

  Fly   797 -------------KHLDTDGLPHPGSKLSYGSPLYCYFDGEVATYKV---------VKMDEKEDC 839
                         :.|..|||...|.|:..|.   .|.:..|.|...         |:...:|..
Yeast   809 MRVDENGDPIWQHQSLGPDGLGEVGMKVQSGQ---IYINKSVPTNSADAPNPNNVNVQTQYREAP 870

  Fly   840 IV------ESIRQ-LGSFDLSPKKMVAITLRVPRPATIGDKFASRAGQKGICSQKYPAEDLPFTE 897
            ::      ..|.| :.|...:.:.::.:.||..|...:||||:||.||||:|......||:||.:
Yeast   871 VIYRGPEPSHIDQVMMSVSDNDQALIKVLLRQNRRPELGDKFSSRHGQKGVCGIIVKQEDMPFND 935

  Fly   898 SGLIPDIVFNPHGFPSRMTIAMMIETMAGKGAAIHGNVYDATPFRFSEENTAIDYFGKMLEAGGY 962
            .|::|||:.||||||||||:..|||.::||...::|.:...|.|..|:    ::...|:|...|:
Yeast   936 QGIVPDIIMNPHGFPSRMTVGKMIELISGKAGVLNGTLEYGTCFGGSK----LEDMSKILVDQGF 996

  Fly   963 NYYGTERLYSGVDGREMTADIFFGVVHYQRLRHMVFDKWQVRSTGAVEARTHQPIKGRKRGGGVR 1027
            ||.|.:.||||:.|..:.|.||||.::||:|:|||.||...|:.|.....|.||.:||.|.||:|
Yeast   997 NYSGKDMLYSGITGECLQAYIFFGPIYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLR 1061

  Fly  1028 FGEMERDALISHGAAFLLQDRLFHNSDKTHTLVCHKCGSILAPLQRIVKRNETGGLSSQPDTCRL 1092
            .||||||.:|::||:.||.:||..:||.....||.||                 ||......|..
Yeast  1062 LGEMERDCVIAYGASQLLLERLMISSDAFEVDVCDKC-----------------GLMGYSGWCTT 1109

  Fly  1093 CGDNSSVSMIEIPFSFKYLVTELSSVNINARFKLNEI 1129
            |....::..:.||::.|.|..||.|:||..|.:|.:|
Yeast  1110 CKSAENIIKMTIPYAAKLLFQELLSMNIAPRLRLEDI 1146

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Polr1B	NP_476708.1	RNA_pol_B_RPB2	35..1125	CDD:238353	330/1185 (28%)
		RNA_pol_Rpa2_4	564..619	CDD:462028	13/54 (24%)
RET1	NP_014850.1	RNA_pol_B_RPB2	57..1141	CDD:238353	331/1192 (28%)
		RNA_pol_Rpb2_4	555..615	CDD:428012	18/66 (27%)
		RNA_pol_Rpb2_5	637..677	CDD:428013	14/40 (35%)