Protein Alignment Polr1B and RPB2

DIOPT Version :10

• Sequence 1: NP_476708.1
• Gene: Polr1B / 33210
• FlyBaseID: FBgn0003278
• Length: 1129
• Species: Drosophila melanogaster
• Sequence 2: NP_014794.3
• Gene: RPB2 / 854322
• SGDID: S000005677
• Length: 1224
• Species: Saccharomyces cerevisiae
• Alignment Length: 1307
• Identity: 354/1307 - (27%)
• Similarity: 559/1307 - (42%)
• Gaps: 362/1307 - (27%)

  Fly    40 VDSFDEML------------TVGLDNSAKHMIPNHWLSPAGEKISMKVE----SIWIAKPKVPQD 88
            :|||::.:            |:.|:..|:|       :...:.||.|.|    .|::.||.|.:.
Yeast    48 LDSFNQFVDYTLQDIICEDSTLILEQLAQH-------TTESDNISRKYEISFGKIYVTKPMVNES 105

  Fly    89 VIDVRTREIYPTDSRQLHVSYSGMCSVRLGWSVNGVQKTPINMD--------------------- 132
              |..|..:||.::|..:::||.      |..|:..::|...:|
Yeast   106 --DGVTHALYPQEARLRNLTYSS------GLFVDVKKRTYEAIDVPGRELKYELIAEESEDDSES 162

  Fly   133 ----LGEVPIMLRSKACNLGQATPEEMVKHGEHDSEWGGIFVIRGNEKIVRMLIMTRRNHPICVK 193
                :|.:|||||||.|.|.:||..::.|..|...:.||.|:|.|:||::           |..:
Yeast   163 GKVFIGRLPIMLRSKNCYLSEATESDLYKLKECPFDMGGYFIINGSEKVL-----------IAQE 216

  Fly   194 RSSWKDRGQNFSDLGMLVQT-----------VREDESSLSNVVHYLNN-------------GTAK 234
            ||:           |.:||.           |.|..|:|.....:::.             .|.|
Yeast   217 RSA-----------GNIVQVFKKAAPSPISHVAEIRSALEKGSRFISTLQVKLYGREGSSARTIK 270

  Fly   235 FMFSHVKRLSYVPVCLILKCLMDYTDEEIYNRL---VQGYESDQYYVSCVQAMLREVQNENVYTH 296
            ....::|:  .:|:.:|.:.|....|.||...:   |..::..:....||:      ....:...
Yeast   271 ATLPYIKQ--DIPIVIIFRALGIIPDGEILEHICYDVNDWQMLEMLKPCVE------DGFVIQDR 327

  Fly   297 AQCKSFIGNLFRA---RFPEVPEWQPDDDVTDFILRERVMIHLDTYED----KFQLIVFMIQKLF 354
            .....|||....|   :..:..::..|      ||::..:.|:...|.    |...:.:||.:|.
Yeast   328 ETALDFIGRRGTALGIKKEKRIQYAKD------ILQKEFLPHITQLEGFESRKAFFLGYMINRLL 386

  Fly   355 QCAQGKYKVENVDSAMMQEVLLPGHLYQKYLSERVESWVSQVRRCLQKKLT-------------- 405
            .||..:...::.|....:.:.|.|.|            ::|:.:.|.||||
Yeast   387 LCALDRKDQDDRDHFGKKRLDLAGPL------------LAQLFKTLFKKLTKDIFRYMQRTVEEA 439

  Fly   406 ---SPDALVTSAVMTQCMRQAGGVGRAIESFLATGN-------IASRTGLGLMQNSGLVIMAENI 460
               :....:.:..:|..::.|          |||||       ::||.|           :::.:
Yeast   440 HDFNMKLAINAKTITSGLKYA----------LATGNWGEQKKAMSSRAG-----------VSQVL 483

  Fly   461 NRMRY---MSHFR----AIHRGSYFTTMRTTEARQLLPDAWGFICPVHTPDGTPCGLLNHLTLTC 518
            ||..|   :||.|    .|.|..     :..:.|||....||.:||..||:|..|||:.:|:|..
Yeast   484 NRYTYSSTLSHLRRTNTPIGRDG-----KLAKPRQLHNTHWGLVCPAETPEGQACGLVKNLSLMS 543

  Fly   519 EISMRPDPKLVKAIPKHLIDMGMMPLSNRRYLGEKLYV---------VFLDGKHLGHIHQSEAEK 574
            .||:..||   ..|...|.:.||.||.:        ||         ||::|...| :|::.| :
Yeast   544 CISVGTDP---MPIITFLSEWGMEPLED--------YVPHQSPDATRVFVNGVWHG-VHRNPA-R 595

  Fly   575 IVDELRYGKIFGTL-PQMMEIGFIPFKKNGQFPGLYIATGPARMMRPVW-----------NLKWK 627
            :::.||..:..|.: |::..|..|..|:      |.|.|...|:.||::           .||.:
Yeast   596 LMETLRTLRRKGDINPEVSMIRDIREKE------LKIFTDAGRVYRPLFIVEDDESLGHKELKVR 654

  Fly   628 R----------------------------------VEYIGTLEQLYMEIAIDAKEMYP------- 651
            :                                  ||||...|:..:.||:..:::.|
Yeast   655 KGHIAKLMATEYQDIEGGFEDVEEYTWSSLLNEGLVEYIDAEEEESILIAMQPEDLEPAEANEEN 719

  Fly   652 DFT----------------THLELAKTHFMSNLANLIPMPDYNQSPRNMYQCQMGKQTMGTPCLN 700
            |..                ||.|:..:..:...|::||.||:||||||.||..||||.||....|
Yeast   720 DLDVDPAKRIRVSHHATTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQAMGVFLTN 784

  Fly   701 WPKQ---AANKLYRLQTP-GTPLFRPVHYDNIQLDDFAMGTNAIVAVISYTGYDMEDAMIINKAA 761
            :..:   .||.||..|.| ||.  |.:.|  ::..:...|.|||||:..|:||:.||:||:|:::
Yeast   785 YNVRMDTMANILYYPQKPLGTT--RAMEY--LKFRELPAGQNAIVAIACYSGYNQEDSMIMNQSS 845

  Fly   762 YERGFAYGSIYKTKFLTLDKK-----SSYFARHPHMPEL-IKH-----LDTDGLPHPGSKLSYGS 815
            .:||. :.|::...::..:||     :..|.:......| :||     ||.|||..||.::|
Yeast   846 IDRGL-FRSLFFRSYMDQEKKYGMSITETFEKPQRTNTLRMKHGTYDKLDDDGLIAPGVRVS--- 906

  Fly   816 PLYCYFDGE---VATYKVVKMDEK---------------------EDCIVESIRQLGSFDLSPKK 856
                   ||   :.....:..||:                     |:.||:.:  |.:.:....|
Yeast   907 -------GEDVIIGKTTPISPDEEELGQRTAYHSKRDASTPLRSTENGIVDQV--LVTTNQDGLK 962

  Fly   857 MVAITLRVPRPATIGDKFASRAGQKGICSQKYPAEDLPFTESGLIPDIVFNPHGFPSRMTIAMMI 921
            .|.:.:|..:...||||||||.||||.....|..||:|||..|::||::.|||..|||||:|.:|
Yeast   963 FVKVRVRTTKIPQIGDKFASRHGQKGTIGITYRREDMPFTAEGIVPDLIINPHAIPSRMTVAHLI 1027

  Fly   922 ETMAGKGAAIHGNVYDATPFRFSEENTAIDYFGKMLEAGGYNYYGTERLYSGVDGREMTADIFFG 986
            |.:..|.||:.||..||:||    .:..::...|:|...||...|.|.:|:|..|:::.|.||||
Yeast  1028 ECLLSKVAALSGNEGDASPF----TDITVEGISKLLREHGYQSRGFEVMYNGHTGKKLMAQIFFG 1088

  Fly   987 VVHYQRLRHMVFDKWQVRSTGAVEARTHQPIKGRKRGGGVRFGEMERDALISHGAAFLLQDRLFH 1051
            ..:||||||||.||...|:.|.::..|.||::||.|.||:||||||||.:|:||||..|::||..
Yeast  1089 PTYYQRLRHMVDDKIHARARGPMQVLTRQPVEGRSRDGGLRFGEMERDCMIAHGAASFLKERLME 1153

  Fly  1052 NSDKTHTLVCHKCGSILAPLQRIVKRNETGGLSSQPDTCRLCGDNSSVSMIEIPFSFKYLVTELS 1116
            .||.....:|..||     |..::.:     |:.....|:.|.:...:..|.||::.|.|..||.
Yeast  1154 ASDAFRVHICGICG-----LMTVIAK-----LNHNQFECKGCDNKIDIYQIHIPYAAKLLFQELM 1208

  Fly  1117 SVNINAR 1123
            ::||..|
Yeast  1209 AMNITPR 1215

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Polr1B	NP_476708.1	RNA_pol_B_RPB2	35..1125	CDD:238353	354/1307 (27%)
		RNA_pol_Rpa2_4	564..619	CDD:462028	14/55 (25%)
RPB2	NP_014794.3	Ricin_B_lectin	31..1216	CDD:481363	354/1307 (27%)