DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RpI135 and Polr3b

DIOPT Version :9

Sequence 1:	NP_476708.1	Gene:	RpI135 / 33210	FlyBaseID:	FBgn0003278	Length:	1129	Species:	Drosophila melanogaster
Sequence 2:	NP_081699.1	Gene:	Polr3b / 70428	MGIID:	1917678	Length:	1133	Species:	Mus musculus

Alignment Length:	1208	Identity:	349/1208 - (28%)
Similarity:	551/1208 - (45%)	Gaps:	187/1208 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly    10 TIPVLTNSRPEFKQIPKKLSRHLANLGGPHVDSFDEMLTVGLDNSAKHMIPNHWLSPAGEKISMK 74
            |.|:.| ...:::.:|..|.  :..|...|:|||:..:.|.:....|          |.||::..
Mouse    17 TAPIPT-VEEKWRLLPAFLK--VKGLVKQHIDSFNYFINVEIKKIMK----------ANEKVTSD 68

  Fly    75 VESIWIAK--------PKVPQDVIDVRTREIYPTDSRQLHVSYSGMCSVRLGWSVNGVQKTPIN- 130
            .:.:|..|        |.| ::..:| ||.:.|.:.|...::||...:|.:.:: .|.|:...|
Mouse    69 ADPMWYLKYLNIYVGLPDV-EESFNV-TRPVSPHECRLRDMTYSAPITVDIEYT-RGSQRIIRNA 130

  Fly   131 MDLGEVPIMLRSKACNLGQATPEEMVKHGEHDSEWGGIFVIRGNEKIVRMLIMTRRNHPICVKRS 195
            :.:|.:||||||..|.|...||.|..|..|...:.||.|:::|.||::.:.....:|..|.
Mouse   131 LPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIV---- 191

  Fly   196 SWKDRGQNFSDLGMLVQTVREDESSLSNVVHYLNNGTAKFMFSHVKRLSYVPVCLILKCLMDYTD 260
                .......:|..|.:...::.|.:|:.  :..|  :|...|......:|:.:|.|.:...:|
Mouse   192 ----EADRKGAVGASVTSSTHEKKSRTNMA--VKQG--RFYLRHNTLSEDIPIVIIFKAMGVESD 248

  Fly   261 EEIYNRLVQGYESDQYYVSCVQAMLREVQNENVYTHAQCKSFIGNLFR-----------ARFPEV 314
            :||    ||...::::.::.....|.|.|...::|..|...:|||..|           .:..|.
Mouse   249 QEI----VQMIGTEEHVMAAFGPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEA 309

  Fly   315 PEWQPDDDVTDFILRERVMIHLDTYEDKFQ----LIVFMIQKLFQCAQGKYKVENVDSAMMQEVL 375
            .|          :|...::.|:...|..|:    ....|::::. .|||..||::.|....:.:.
Mouse   310 RE----------LLASTILTHVPVKEFNFRAKCIYTAVMVRRVI-LAQGDNKVDDRDYYGNKRLE 363

  Fly   376 LPGHLYQKYLSERVESWVSQVRRCLQKKLTSPDALVTSAVMTQCMRQ---AGGVGRAIESFLATG 437
            |.|.|......:..:.:.|::::...:.:  |........:.:.|||   ..|:..||    :||
Mouse   364 LAGQLLSLLFEDLFKKFNSEMKKIADQVI--PKQRAAQFDVVKHMRQDQITNGMVNAI----STG 422

  Fly   438 NIASRTGLGLMQNSGLVIMAENINRMRYMSHFRAIHR--GSYFTTMRTTEARQLLPDAWGFICPV 500
            |.:.:.  ..|...|   :.:.::|:.|:|....:.|  ..:..|.:.:..|.|.|..||.:||.
Mouse   423 NWSLKR--FKMDRQG---VTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPS 482

  Fly   501 HTPDGTPCGLLNHLTLTCEIS--MRPDPKLVKAIPKHLIDMGMMPLSNRRYLGEKL-----YVVF 558
            .||:|..|||:.:|.|...|:  |...|.:..|....:.|:.::       .||:|     ::||
Mouse   483 DTPEGEACGLVKNLALMTHITTDMEDGPIIKLAGNLGVEDVNLL-------CGEELSYPNVFLVF 540

  Fly   559 LDGKHLGHIHQSEAEKIVDELRYGKIFGTLPQMMEIGFIPFKKNGQFPGLYIATGPARMMRPVWN 623
            |:|..||.|.  :.:|:|...|..:..|.:.:     |:....|.....:||::...|:.||...
Mouse   541 LNGNILGVIR--DHKKLVSTFRLMRRAGYINE-----FVSISTNLTDRCVYISSDGGRLCRPYII 598

  Fly   624 LKWKR--------------------------VEYIGTLEQLYMEIAIDAKEMYPDFTTHLELAKT 662
            :|.::                          |||:...|:....||:....:..| |||||:...
Mouse   599 VKKQKPAVTNKHMEELAQGYRNFEDFLHESLVEYLDVNEENDCNIALYEHTINKD-TTHLEIEPF 662

  Fly   663 HFMSNLANLIPMPDYNQSPRNMYQCQMGKQTMGTPCLNWPKQAANKLYRLQTPGTPLFRPVHYDN 727
            ..:...|.|||.|.:||||||.|||.||||.|||...|...:....:|.|..|..|:.:....:.
Mouse   663 TLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIEL 727

  Fly   728 IQLDDFAMGTNAIVAVISYTGYDMEDAMIINKAAYERGFAYGSIYKTKFLTLDKKSSY------- 785
            |..:....|.||.|||:||:|||:|||:::|||:.:|||....:||....||.:.::.
Mouse   728 IDFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMG 792

  Fly   786 -----FARHPHMPELIKH--LDTDGLPHPGSKL---------SYGSPLYCYFDG----EVATYKV 830
                 ..|.|    :.:|  ||.||:..||.|:         |..:......:|    :...||.
Mouse   793 PMLDAATRKP----IWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKD 853

  Fly   831 VKMDEK--EDCIVESIRQLGSFDLSPKKMVAITLRVPRPATIGDKFASRAGQKGICSQKYPAEDL 893
            |.:..|  .|..:|.:  :.|.:.....::.:.||..|...|||||:||.||||:|....|.||:
Mouse   854 VPITYKGATDSYIEKV--MISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDM 916

  Fly   894 PFTESGLIPDIVFNPHGFPSRMTIAMMIETMAGKGAAIHGNVYDATPFRFSEENTAIDYFGKMLE 958
            ||.:||:.|||:.||||||||||:..:||.:|||...:.|..:..|.|..|:.....:    .|.
Mouse   917 PFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCE----DLV 977

  Fly   959 AGGYNYYGTERLYSGVDGREMTADIFFGVVHYQRLRHMVFDKWQVRSTGAVEARTHQPIKGRKRG 1023
            ..||||.|.:.:.||:.|..:.|.|:||.|:||:|:|||.||...|:.|.....|.||.:||.|.
Mouse   978 RHGYNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRD 1042

  Fly  1024 GGVRFGEMERDALISHGAAFLLQDRLFHNSDKTHTLVCHKCGSILAPLQRIVKRNETGGLSSQPD 1088
            ||:|.||||||.||.:||:.||.:||..:||.....||.:||.:              |.|.
Mouse  1043 GGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLL--------------GYSG--- 1090

  Fly  1089 TCRLCGDNSSVSMIEIPFSFKYLVTELSSVNINARFKL 1126
            .|..|..:..||.:.||::.|.|..||.|:||..|.||
Mouse  1091 WCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKL 1128

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RpI135	NP_476708.1	RpoB	34..1128	CDD:223163	344/1184 (29%)
		RNA_pol_B_RPB2	35..1125	CDD:238353	342/1180 (29%)
		RNA_pol_Rpa2_4	564..614	CDD:284335	11/49 (22%)
Polr3b	NP_081699.1	PRK08565	26..1129	CDD:236291	346/1198 (29%)
		RNA_pol_B_RPB2	39..1127	CDD:238353	342/1180 (29%)
		RNA_pol_Rpb2_4	539..600	CDD:282431	18/67 (27%)
		RNA_pol_Rpb2_5	621..661	CDD:282432	12/40 (30%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0085
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D42570at2759
OrthoFinder	1	1.000	-	-		FOG0000346
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	4	3.820