DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RpI135 and polr3b

DIOPT Version :9

Sequence 1:	NP_476708.1	Gene:	RpI135 / 33210	FlyBaseID:	FBgn0003278	Length:	1129	Species:	Drosophila melanogaster
Sequence 2:	XP_021323450.1	Gene:	polr3b / 562329	ZFINID:	ZDB-GENE-030131-2887	Length:	1130	Species:	Danio rerio

Alignment Length:	1230	Identity:	359/1230 - (29%)
Similarity:	557/1230 - (45%)	Gaps:	209/1230 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MLEE-----MQQMKTIPVLTNSRPEFKQIPKKLSRHLANLGGPHVDSFDEMLTVGLDNSAKHMIP 60
            ||:|     ..|....||.| ...::|.:|..|.  :..|...|:|||:..:.|.:....|
Zfish     1 MLQEEFGEMSPQQLAAPVDT-VEEKWKLLPAFLK--VKGLVKQHIDSFNYFINVEIKKIMK---- 58

  Fly    61 NHWLSPAGEKISMKVESIWIAK--------PKVPQDVIDVRTREIYPTDSRQLHVSYSGMCSVRL 117
                  |.|||:...:.:|..|        |.| ::..:| ||.:.|.:.|...::||...:|.:
Zfish    59 ------ANEKITSDADPMWYLKYLNIYVGMPDV-EESFNV-TRPVSPHECRLRDMTYSAPITVDI 115

  Fly   118 GWSVNGVQKTPIN-MDLGEVPIMLRSKACNLGQATPEEMVKHGEHDSEWGGIFVIRGNEKIVRML 181
            .:: .|.|:...| :.:|.:||||||..|.|...||.|..|..|...:.||.|:::|.||::.:.
Zfish   116 EYT-RGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPMEFSKLNECPLDPGGYFIVKGQEKVILIQ 179

  Fly   182 IMTRRNHPICVKRSSWKDRGQNFSDLGMLVQTVREDESSLSNVVHYLNNGTAKFMFSHVKRLSYV 246
            ....:|..|.       |:.:. ..:|..|.:...::.|.:|::  :..|  :|...|.......
Zfish   180 EQLSKNRIIV-------DQDRK-GTVGASVTSSTHEKKSRTNMI--VKQG--RFYLKHNTLSEDA 232

  Fly   247 PVCLILKCLMDYTDEEIYNRLVQGYESDQYYVSCVQAMLREVQNENVYTHAQCKSFIGN-LFRAR 310
            |:.:|.|.:...:|:||    ||...::::.::.....|.|.|...::|..|...:||| :.|.|
Zfish   233 PIAIIFKAMGVESDQEI----VQMIGTEEHVMAAFAPSLEECQKAQIFTQTQALRYIGNKVRRQR 293

  Fly   311 FPEVPEWQPDDDVTDFILRERVMIHLDTYEDKFQ----LIVFMIQKLFQCAQGKYKVENVDSAMM 371
            ....|:....::..: :|...::.|:...|..|:    .:..|::::. .|||..||::.|....
Zfish   294 MWGGPKKTKMEEARE-LLASTILTHVPVKEFNFRAKCIYLAVMVRRVI-LAQGDNKVDDRDYYGN 356

  Fly   372 QEVLLPGHLYQKYLSERVESWVSQVRRCLQKKLTSPDALVTSAVMTQCMRQ---AGGVGRAIESF 433
            :.:.|.|.|......:..:.:.|::::...:.:  |........:.:.|||   ..|:..||
Zfish   357 KRLELAGQLLSLLFEDLFKKFNSELKKIADQII--PKQRAAQFDVVKHMRQDQITNGMVNAI--- 416

  Fly   434 LATGNIASRTGLGLMQNSGLVIMAENINRMRYMSHFRAIHR--GSYFTTMRTTEARQLLPDAWGF 496
             :|||.:.:.  ..|...|   :.:.::|:.::|....:.|  ..:..|.:.:..|.|.|..||.
Zfish   417 -STGNWSLKR--FKMDRQG---VTQVLSRLSFISALGMMTRISSQFEKTRKVSGPRSLQPSQWGM 475

  Fly   497 ICPVHTPDGTPCGLLNHLTLTCEIS--MRPDPKLVKAIPKHLIDMGMMPLSNRRYLGEKL----- 554
            :||..||:|..|||:.:|.|...|:  |...|.:..|....:.|:.::       .||:|
Zfish   476 LCPSDTPEGEACGLVKNLALMTHITTDMEDGPIIKLAFNLGVEDVNLL-------CGEELSYPTV 533

  Fly   555 YVVFLDGKHLGHIHQSEAEKIVDELRYGKIFGTLPQMMEIGFIPFKKNGQFPGLYIATGPARMMR 619
            ::|||:|..||.|.  :.:|:|...|..:..|.:.:     |:....|.....:||::...|:.|
Zfish   534 FLVFLNGNILGVIR--DHQKLVYTFRLMRRAGFINE-----FVSISTNLTDRCVYISSDGGRLCR 591

  Fly   620 P----------VWN----------------LKWKRVEYIGTLEQLYMEIAIDAKEMYPDFTTHLE 658
            |          |.|                |....|||:...|:....||: .:.|....|||||
Zfish   592 PYIIVKNGKPAVKNKHIEELSQGYRTFEDFLHESLVEYLDVNEENDCNIAL-YEHMISKDTTHLE 655

  Fly   659 LAKTHFMSNLANLIPMPDYNQSPRNMYQCQMGKQTMGTPCLNWPKQAANKLYRLQTPGTPLFRPV 723
            :.....:...|.|||.|.:||||||.|||.||||.|||...|...:....:|.|..|..|:.:..
Zfish   656 IEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQRPMVKTK 720

  Fly   724 HYDNIQLDDFAMGTNAIVAVISYTGYDMEDAMIINKAAYERGFAYGSIYKTKFLTLDKKSSYF-- 786
            ..:.|..:....|.||.|||:||:|||:|||:::|||:.:|||....:||....||.:.::..
Zfish   721 TIELIDFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLRRYTNQTFD 785

  Fly   787 ----------ARHPHMPELIKH--LDTDGLPHPGSKLSY-----------------------GSP 816
                      .|.|    :.:|  ||.||:..||.|:..                       |.|
Zfish   786 KVMGPMLDAETRKP----IWRHSILDADGICSPGEKVENKQVLVNKSMPTVTQTPLEGSAQPGQP 846

  Fly   817 LY----CYFDGEVATY--KVVKMDEKEDCIVESIRQLGSFDLSPKKMVAITLRVPRPATIGDKFA 875
            .|    ..:.|...:|  ||:.....||..                :|.|.||..|...|||||:
Zfish   847 QYREVPVSYKGATDSYIEKVMISSNAEDAF----------------LVKILLRQTRRPEIGDKFS 895

  Fly   876 SRAGQKGICSQKYPAEDLPFTESGLIPDIVFNPHGFPSRMTIAMMIETMAGKGAAIHGNVYDATP 940
            ||.||||:|....|.||:||.:||:.|||:.||||:|||||:..:||.:|||...:.|..:..|.
Zfish   896 SRHGQKGVCGLIVPQEDMPFCDSGICPDIIMNPHGYPSRMTVGKLIELLAGKAGVLDGRFHYGTA 960

  Fly   941 FRFSEENTA----IDYFGKMLEAGGYNYYGTERLYSGVDGREMTADIFFGVVHYQRLRHMVFDKW 1001
            |..|:....    |.|        ||||.|.:.:.||:.|..:.|.|:||.|:||:|:|||.||.
Zfish   961 FGGSKVKDVCEDLIRY--------GYNYQGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKM 1017

  Fly  1002 QVRSTGAVEARTHQPIKGRKRGGGVRFGEMERDALISHGAAFLLQDRLFHNSDKTHTLVCHKCGS 1066
            ..|:.|.....|.||.:||.|.||:|.||||||.||.:||:.||.:||..:||.....||.:||.
Zfish  1018 HARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGL 1082

  Fly  1067 ILAPLQRIVKRNETGGLSSQPDTCRLCGDNSSVSMIEIPFSFKYLVTELSSVNINARFKL 1126
            :              |.|.   .|..|..:..||.:.||::.|.|..||.|:||..|.||
Zfish  1083 L--------------GYSG---WCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKL 1125

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RpI135	NP_476708.1	RpoB	34..1128	CDD:223163	349/1192 (29%)
		RNA_pol_B_RPB2	35..1125	CDD:238353	347/1188 (29%)
		RNA_pol_Rpa2_4	564..614	CDD:284335	11/49 (22%)
polr3b	XP_021323450.1	RNA_pol_B_RPB2	37..1124	CDD:238353	347/1188 (29%)
		RNA_pol_Rpb2_4	536..597	CDD:309626	18/67 (27%)
		RNA_pol_Rpb2_5	618..658	CDD:309627	13/40 (33%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0085
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D42570at2759
OrthoFinder	1	1.000	-	-		FOG0000346
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	4	3.820