Protein Alignment Plc21C and Plcg2

DIOPT Version :10

• Sequence 1: NP_995605.1
• Gene: Plc21C / 33204
• FlyBaseID: FBgn0004611
• Length: 1318
• Species: Drosophila melanogaster
• Sequence 2: NP_058864.1
• Gene: Plcg2 / 29337
• RGDID: 3348
• Length: 1265
• Species: Rattus norvegicus
• Alignment Length: 1261
• Identity: 301/1261 - (23%)
• Similarity: 450/1261 - (35%)
• Gaps: 484/1261 - (38%)

  Fly    52 WVDQNNELD-ILDIATIRDVRTGQYAKRPKDNKLRQIVTLGPQDTLEEKTV--------TVCHGS 107
            |....:::: .|||..|:::|.|:.:|                |....|.|        |:.:|:
  Rat    54 WSKTADKIEGFLDIMEIKEIRPGKNSK----------------DFERAKAVRHKADCCFTIFYGT 102

  Fly   108 DFVNMTFVNFCCTRRDIAQLWTDGLIKLAYSLAQLNGSAIMFLQKAHTKLCLQVDKSGRIPVKNI 172
            .|| ::.::.....::.|..|..|| |:.:..| :|.|....::....|....||::.|    |.
  Rat   103 QFV-LSTLSLATDSKEDAVKWLSGL-KILHQEA-MNASTPTMIESWLRKQIYSVDQTRR----NS 160

  Fly   173 IKLFAQNKEDRKRVEKALD--VTGLP--SGKVDSISVSK--FQFEDFYNLYKYL---TQRSEVER 228
            |.|     .:.|.:...::  |:|:.  ..|:..|...|  ..||.|:..||.|   .|:|.::.
  Rat   161 ISL-----RELKTILPLVNFKVSGIKFLKDKLVEIGAQKDELSFEQFHLFYKKLMFEQQKSILDE 220

  Fly   229 L-FDS---IVGNSKRKCMSIAQLVEF---------------LNKTQR------DPRLNEILYPYA 268
            . .||   |:||:.|...|...|.:|               |||.:.      |..:.|...|:
  Rat   221 FKKDSSVFILGNTDRPDASAVYLQDFQRFLLHEQQELWAQDLNKVRERMTKFIDDTMRETAEPF- 284

  Fly   269 NPARAKELIQQYEPNKFNAQKGQLSLDGFLRYLMGDDNPIMAPSKLDLCD--DMDQPMSHYFINS 331
                                   |.:|.||.||...:|.|. ..|.|..|  ||:.|:|||:|:|
  Rat   285 -----------------------LFVDEFLTYLFSRENSIW-DEKYDAVDMQDMNNPLSHYWISS 325

  Fly   332 SHNTYLTGHQLTGKSSVEIYRQCLLAGCRCVELDFWNGRTEEPVIVHGYTFVPEIFAKDVLEAIA 396
            ||||||||.||..:||.|.|.:||.|||||:|||.|:|...:|:|.||:|...:|...||::||.
  Rat   326 SHNTYLTGDQLRSESSTEAYIRCLRAGCRCIELDCWDGPDGKPIIYHGWTRTTKIKFDDVVQAIR 390

  Fly   397 ESAFKTSEYPVILSFENHCNPRQQAKIANYCREIFGDMLLDKPLDSHPLEPNMD-LPPPAMLRRK 460
            :.||.||.:|||||.|.||:..||..:|...:|:.||:||.|     |.|.:.| ||.|:.||.|
  Rat   391 DHAFVTSSFPVILSIEEHCSVEQQRHMAKVFKEVLGDLLLTK-----PTEASADQLPSPSQLREK 450

  Fly   461 IIIKNK--------------KKHHHHHH--------------HHH------------------HK 479
            ||||:|              ||..|...              .|:                  .:
  Rat   451 IIIKHKKLGPRGDVDVNVEDKKDEHKTQGELYMWDSIDQKWTRHYCAIADAKLSFSDDIEQTVEE 515

  Fly   480 KPAQVGTPAA--------NNKLTTANSVDAKAAQQVGLSASHEDGGV------------------ 518
            .|.|...|..        :.|:.:..|.: |..|:.......:||..
  Rat   516 DPVQDTPPTELHFGEKWFHKKVESRTSAE-KLLQEYCAETGAKDGTFLVRESETFPNDYTLSFWR 579

  Fly   519 --------TRSTANGDV------------------------------------------------ 527
                    .|||..|.|
  Rat   580 SGRVQHCRIRSTMEGGVMKYYLTDNLTFNSIYALIQHYREAHLRCAEFELRLTDPVPNPNPHESK 644

  Fly   528 -------ATG--------------------TGTGSAA-----------------------GTAG- 541
                   :.|                    .||.|.|                       ||:.
  Rat   645 PWYYDRLSRGEAEDMLMRIPRDGAFLIRKREGTDSYAITFRARGKVKHCRINRDGRHFVLGTSAY 709

  Fly   542 -------------HA------------------------------------------PPLQQ--- 548
                         ||                                          |.:.|
  Rat   710 FESLVELVSYYEKHALYRKMRLRYPVTPELLERYNMERDINSLYDVSRMYVDPSEINPSMPQRTV 774

  Fly   549 ---------------------IRQSSKDSTG---------------------SSDSDSSSEDESL 571
                                 |...||:..|                     .|..|:...::.:
  Rat   775 KALYDYKAKRSDELTFCRGALIHNVSKEPGGWWKGDYGTRIQQYFPSNYVEDISAGDAEEMEKQI 839

  Fly   572 PNTTP-----------------NLPSG---------------NEPPPEKA--------------- 589
            ....|                 ..|.|               .:||.|.|
  Rat   840 IEDNPLGSLCRGILDLNTYNVVKAPQGKNQKAFVFILEPKKQGDPPVEFATDRVEELFEWFQSIR 904

  Fly   590 ----------------QKETEAGAEISALVNYVQPIHFSS--FENAEKKNRCYEMSSFDEKQATT 636
                            ::......|:|.||.|.:|...:.  .||.:.:    |:.||.|.:|.:
  Rat   905 EITWKIDTKENNMKYWERNQSIAIELSDLVVYCKPTSKTKDHLENPDFR----EIRSFVETKADS 965

  Fly   637 LLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNFQTLDLAMQLNLGIFEY 701
            :::::|::.:.||:..|:||||.|.|.||||:.|...|..|.|:|||||||.|..||:|..:|..
  Rat   966 IVRQKPVDLLRYNQKGLTRVYPKGQRVDSSNYDPFRLWLCGSQMVALNFQTPDKYMQMNHALFSL 1030

  Fly   702 NARSGYLLKPEFMRRSDRRLDPFAESTVDGIIAGTVSITVLSGQFLTD---KRANTFVEVDMYGL 763
            |.|:||:|:||.||  ..:.||....:...|:. |:::.||..:.|..   ..|..||||::.|.
  Rat  1031 NGRTGYVLQPESMR--SEKYDPMPPESQRKILM-TLTVKVLGARHLPKLGRSIACPFVEVEICGA 1092

  Fly   764 PADTVRKKFRTKTVRDNGMNPLY--DEEPFVFKKVVLPELASIRIAAYEE----GGKLIGHRVLP 822
            ..|:  .||:|..|.|||::|::  .:|...| ::..|.||.:|...|||    ....:.|...|
  Rat  1093 EYDS--NKFKTTVVNDNGLSPVWAPTQEKVTF-EIYDPNLAFLRFLVYEEDMFSDPNFLAHATYP 1154

  Fly   823 VIGLCPGYRHVNLRSEVGQPIALASLFLCVVVKDYVPDDLSNFAEALANPIKYQS--ELEKRDIQ 885
            :.|:..|:|.|.|::...:.|.||||.:...::..:..:     |.|     |.|  :|.:|..:
  Rat  1155 IKGIKSGFRSVPLKNGYSEDIELASLLVFCEMRPVLESE-----EEL-----YSSCRQLRRRQEE 1209

  Fly   886 LS---VLTDEAEALGSADED-LSKSF 907
            |:   .|.|..:.|..|:.| |.|.|
  Rat  1210 LNNQLFLYDTHQNLRGANRDALVKEF 1235

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Plc21C	NP_995605.1	PH_PLC_beta	20..145	CDD:270167	22/101 (22%)
		EFh_PI-PLC21	151..305	CDD:320043	42/187 (22%)
		EF-hand motif	151..180	CDD:320043	7/28 (25%)
		EF-hand motif	187..216	CDD:320043	8/34 (24%)
		EF-hand motif	225..255	CDD:320043	12/48 (25%)
		EF-hand motif	272..305	CDD:320043	6/32 (19%)
		PI-PLCc_beta	317..702	CDD:176533	165/731 (23%)
		C2_PLC_like	736..855	CDD:175974	40/127 (31%)
		PTZ00121	<981..>1253	CDD:173412
Plcg2	NP_058864.1	PH_PLC_gamma	22..140	CDD:270168	23/104 (22%)
		EFh_PI-PLCgamma2	145..298	CDD:320045	42/185 (23%)
		PI-PLCc_gamma	311..>458	CDD:176534	79/151 (52%)
		PH-like	475..>510	CDD:473070	2/34 (6%)
		SH2_N-SH2_PLC_gamma_like	527..627	CDD:199829	11/100 (11%)
		SH2_C-SH2_PLC_gamma_like	641..744	CDD:198186	9/102 (9%)
		SH3_PLCgamma2	773..827	CDD:212902	4/53 (8%)
		PHsplit_PLC_gamma	<840..910	CDD:270054	7/69 (10%)
		PI-PLCc_GDPD_SF	<926..1031	CDD:472694	43/108 (40%)
		C2_PLC_like	1061..1187	CDD:175974	40/129 (31%)