Protein Alignment Plc21C and Plcb3

DIOPT Version :10

• Sequence 1: NP_995605.1
• Gene: Plc21C / 33204
• FlyBaseID: FBgn0004611
• Length: 1318
• Species: Drosophila melanogaster
• Sequence 2: NP_032900.2
• Gene: Plcb3 / 18797
• MGIID: 104778
• Length: 1234
• Species: Mus musculus
• Alignment Length: 1353
• Identity: 533/1353 - (39%)
• Similarity: 738/1353 - (54%)
• Gaps: 197/1353 - (14%)

  Fly    16 VPQALQDGEKFIRWDDDSGTGTPVTMRVDAKGFFLYWVDQNNELDILDIATIRDVRTGQYAKRPK 80
            |.:.|:.|.|||:||:::.:...||:|||..||||||...|.|:|.|||::|||.|||:||:.||
Mouse    18 VVETLRRGSKFIKWDEEASSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIRDTRTGRYARLPK 82

  Fly    81 DNKLRQIVTLGPQDT-LEEKTVTVCHGSDFVNMTFVNFCCTRRDIAQLWTDGLIKLAYSLAQLNG 144
            |.|:|:::..|..|| ||||.:||..|.|.||.||:||...:.|..::|::.|.|||.::...|.
Mouse    83 DPKIREVLGFGGPDTRLEEKLMTVVAGPDPVNTTFLNFMAVQDDTVKVWSEELFKLAMNILAQNA 147

  Fly   145 SAIMFLQKAHTKLCLQVDKSGRIPVKNIIKLFAQNKEDRKRVEKALDVTGLPSGKVDSISVSKFQ 209
            |...||:||:|||.|||::.||||||||:|:|:   .|:||||.||:..||...:|:||...:|.
Mouse   148 SRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFS---ADKKRVETALESCGLNFNRVESIRPDEFP 209

  Fly   210 FEDFYNLYKYLTQRSEVERLFDSIVGNSKRKCMSIAQLVEFLNKTQRDPRLNEILYPYANPARAK 274
            .|.|......|..|.:::::...| |...:..:::.||::|:|:.||||||||:|||....::|:
Mouse   210 LEIFERFLNKLCLRPDIDKILLEI-GAKGKPYLTLEQLMDFINQKQRDPRLNEVLYPPLRSSQAR 273

  Fly   275 ELIQQYEPNKFNAQKGQLSLDGFLRYLMGDDNPIMAPSKLDLCDDMDQPMSHYFINSSHNTYLTG 339
            .||::||.||...::.|:|::||.|||.|::|.|:....|||..||.||:|.||||||||||||.
Mouse   274 LLIEKYETNKQFLERDQMSMEGFSRYLGGEENGILPLEALDLSMDMTQPLSAYFINSSHNTYLTA 338

  Fly   340 HQLTGKSSVEIYRQCLLAGCRCVELDFWNGR--TEEPVIVHGYTFVPEIFAKDVLEAIAESAFKT 402
            .||.|.||||:|||.||.||||||||.|.||  .|||.|.||:|...|:..:||||||||:||||
Mouse   339 GQLAGPSSVEMYRQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAEAAFKT 403

  Fly   403 SEYPVILSFENHC-NPRQQAKIANYCREIFGDMLLDKPLDSHPLEPNMDLPPPAMLRRKIIIKNK 466
            |.||||||||||. :.:||||:|.|||.||||.||..|||.:||...:.||.|..|..:|::|||
Mouse   404 SPYPVILSFENHVDSAKQQAKMAEYCRSIFGDALLIDPLDKYPLSAGIPLPSPQDLMGRILVKNK 468

  Fly   467 KKHHHHHHHHHHKKPAQVGTPAAN---NKLTTANSVDAKAAQQVGLSASHEDGGVTRSTANGDVA 528
            |:|          :|: .|.|.::   ..|..:||..:::      ||:.|.......:.:.|..
Mouse   469 KRH----------RPS-TGVPDSSVRKRPLEQSNSALSES------SAATEPSSPQLGSPSSDSC 516

  Fly   529 TGTGTGSAAGTAGHAPPLQQIRQSSKDSTGSS-------------DSDSSSEDESLPNTT-PNLP 579
            .|...|...|       |::.....:.|.|..             |.:...|||....|| |..|
Mouse   517 PGLSNGEEVG-------LEKTSLEPQKSLGEESLSREPNVPMPDRDREDEEEDEEEEETTDPKKP 574

  Fly   580 SGNEPPPEKAQKETEAGAEISALVNYVQPIHFSSFENAEKKNRCYEMSSFDEKQATTLLKERPIE 644
            :.:|   ..|..|..|..|:|.|||||:|:.|.|||.|.|:|:|:|||||.|.:|...|.:.|:|
Mouse   575 TTDE---GTASSEVNATEEMSTLVNYVEPVKFKSFEAARKRNKCFEMSSFVETKAMEQLTKSPME 636

  Fly   645 FVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNFQTLDLAMQLNLGIFEYNARSGYLL 709
            ||.|||.||||:||.|||.||||:|||||||.||||||||||||||.||||.|:||||.||||||
Mouse   637 FVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDLPMQLNAGVFEYNGRSGYLL 701

  Fly   710 KPEFMRRSDRRLDPFAESTVDGIIAGTVSITVLSGQFLTDKRANTFVEVDMYGLPADTVRKKFRT 774
            |||||||.|:..|||.|..||||:|..:.:.|:|||||:||:...:|||||:|||.|| |:|:||
Mouse   702 KPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDKKVGIYVEVDMFGLPVDT-RRKYRT 765

  Fly   775 KTVRDNGMNPLYDEEPFVFKKVVLPELASIRIAAYEEGGKLIGHRVLPVIGLCPGYRHVNLRSEV 839
            :|.:.|..||::|||||.|.|||||.|||:||||:|||||.:|||:|||..:..||.:|.||:|.
Mouse   766 RTSQGNSFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEA 830

  Fly   840 GQPIALASLFLCVVVKDYVPDDLSNFAEALANPIKYQSELEKRDIQLSVLTDEAEALGSADEDLS 904
            .||:.|.:|.:.....||:|||..::||||.||||:.|.:::|..||:.|..|:||..|.:
Mouse   831 NQPLCLPALLIYTEASDYIPDDHQDYAEALINPIKHVSLMDQRAKQLAALIGESEAQASTE---- 891

  Fly   905 KSFVFVQVGGQKKELRPVESLATSPKHRPSISAAAAMSVDVTVDRTDGGRGEDSISIVAPSIQHQ 969
               .:.:...|    :|...|.::|...| :.|:.......|...|       |.|:.:|.
Mouse   892 ---TYQETPCQ----QPGSQLPSNPTPNP-LDASPRWPPGPTTSST-------SSSLSSPG---- 937

  Fly   970 HSLDQSVSTSIRQVESSQFDVDLVLAEPLEKILDHKSVKEKRLEMEKKLESLRKKHDKEKIKIAG 1034
             ..|..:::.:.:|..:          |||::..||::.:.|...::.|..|.|||.::.:
Mouse   938 -QRDDLIASILSEVTPT----------PLEELRSHKAMVKLRSRQDRDLRELHKKHQRKAV---- 987

  Fly  1035 QKSSPLEGKKPKFAITNKLVKRLSNKSLNCLSPHSEPGVEIPACPLD-LGDSSEESAAADAGEDL 1098
                         |:|.:|                          || |..:..|.....:...|
Mouse   988 -------------ALTRRL--------------------------LDGLAQARAEGKCRPSPSAL 1013

  Fly  1099 AGGSSSLDGRTQESRLRSACREYTSQYRELQEKYHEAIYSAAE---------------------- 1141
            ...::|.|.:.:|.         ..||||.|.:..:::....|
Mouse  1014 GKATNSEDVKEEEE---------AKQYREFQNRQVQSLLELREAQADVETKRKLEHLRQAHQRLK 1069

  Fly  1142 KVLKTSQTGQTKQLKASLDKVTGEVMHQLQEARRNEVKNLAT--VHRDRDELIRMKREVASSVVE 1204
            :|:..:.|.|.|:||...::...|:...|...|.|.:....|  .|:...||..:.|
Mouse  1070 EVVLDTHTTQFKRLKELNEREKKELQKILDRKRNNSISEAKTREKHKKEVELTEINR-------- 1126

  Fly  1205 RGVAERV----RLKQTFDRRTDELQKQHDSVRNALAEHRSKARQILDKEA---------ESRSCV 1256
            |.:.|.|    ||::...:|.:.|......|...|.|...|....|.:|.         |.|.|:
Mouse  1127 RHITESVNSIRRLEEAQKQRHERLVAGQQQVLQQLEEEEPKLLAQLTQECQEQRERLPQEIRRCL 1191

  Fly  1257 SSNGFLVLFHGPHHHGCTGSGSSALSGNNLTLNLDAGAAGSHSAISPAKSHNS 1309
            .......|..|| ...|..:|.:..||.:|:           ||.|.::..|:
Mouse  1192 LGETAEGLGDGP-LVACASNGHAPGSGGHLS-----------SADSESQEENT 1232

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Plc21C	NP_995605.1	PH_PLC_beta	20..145	CDD:270167	61/125 (49%)
		EFh_PI-PLC21	151..305	CDD:320043	67/153 (44%)
		EF-hand motif	151..180	CDD:320043	18/28 (64%)
		EF-hand motif	187..216	CDD:320043	11/28 (39%)
		EF-hand motif	225..255	CDD:320043	6/29 (21%)
		EF-hand motif	272..305	CDD:320043	15/32 (47%)
		PI-PLCc_beta	317..702	CDD:176533	200/404 (50%)
		C2_PLC_like	736..855	CDD:175974	65/118 (55%)
		PTZ00121	<981..>1253	CDD:173412	58/309 (19%)
Plcb3	NP_032900.2	PH_PLC_beta	22..149	CDD:270167	61/126 (48%)
		EFh_PI-PLCbeta3	154..304	CDD:320040	67/153 (44%)
		EF-hand motif	154..183	CDD:320040	18/31 (58%)
		EF-hand motif	187..216	CDD:320040	11/28 (39%)
		EF-hand motif	225..254	CDD:320040	6/29 (21%)
		EF-hand motif	271..304	CDD:320040	15/32 (47%)
		PI-PLCc_beta	316..694	CDD:176533	200/404 (50%)
		PLN02952	<318..834	CDD:178538	287/543 (53%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	466..588		32/148 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	888..938		12/73 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1001..1026		4/24 (17%)
		PLC-beta_C	1029..1202	CDD:462571	38/180 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1207..1234		9/37 (24%)
		Interaction with SHANK2. /evidence=ECO:0000250	1231..1234		1/2 (50%)