Protein Alignment RunxB and RUNX2

DIOPT Version :10

• Sequence 1: NP_001259745.1
• Gene: RunxB / 33051
• FlyBaseID: FBgn0259162
• Length: 663
• Species: Drosophila melanogaster
• Sequence 2: NP_001019801.3
• Gene: RUNX2 / 860
• HGNCID: 10472
• Length: 521
• Species: Homo sapiens
• Alignment Length: 687
• Identity: 200/687 - (29%)
• Similarity: 277/687 - (40%)
• Gaps: 233/687 - (33%)

  Fly     5 AEVSSTTSNQIQQQQQQHQQQQQHQLLQHQQQQTATTTTTKRRNAESSASSNNNNNNNTSTTNNN 69
            ::||...:.|.||||||.|||||.|..|.|||:.|.........|.::|:
Human    40 SDVSPVVAAQQQQQQQQQQQQQQQQQQQQQQQEAAAAAAAAAAAAAAAAA--------------- 89

  Fly    70 NTNNNNNSTTNNNNNNNNNNVKTKPVDTSPYLTPENLIERTVDVLLAEHPGELVKTGSPHVVCTT 134
                                        .|.|.|.: ..||:..::|:||.|||:|.||:.:|:.
Human    90 ----------------------------VPRLRPPH-DNRTMVEIIADHPAELVRTDSPNFLCSV 125

  Fly   135 LPTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELRNCTAVMKNQVAKFNDLRFVGR 199
            ||:|||.|||||:||||:|||||.|||:||:.||||||:..||||.:||||||||:|||||||||
Human   126 LPSHWRCNKTLPVAFKVVALGEVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGR 190

  Fly   200 SGRGKSFTLTIVISTNPIQIATYTKAIKVTVDGPREPRSKVRHQ----GFHPFAFGPQRFG---- 256
            |||||||||||.:.|||.|:|||.:|||||||||||||   ||:    ...|..|..:...
Human   191 SGRGKSFTLTITVFTNPPQVATYHRAIKVTVDGPREPR---RHRQKLDDSKPSLFSDRLSDLGRI 252

  Fly   257 PDPLM-AGLPFKLPGFAHHLVGMHSHLHAPDWRAHMALGGRPAAFTA-APFFGHHAAAFPTASGL 319
            |.|.| .|:|.:.|                          ||:..:| :||
Human   253 PHPSMRVGVPPQNP--------------------------RPSLNSAPSPF-------------- 277

  Fly   320 RGLSGDSQQHQQQQQQHQLATVGAAHSTTSPEGSPTTTTTSGTQLSAFVQPPMTSSPPPVTSLQH 384
             ...|.||....:|.|            :||..|...:..|  .||....|.:.|:.|
Human   278 -NPQGQSQITDPRQAQ------------SSPPWSYDQSYPS--YLSQMTSPSIHSTTP------- 320

  Fly   385 DNNNNNSNNNNSSSHIDAGFESDSISVTGSPRKSLGSPLTHDEEEAEAE-----AEAEAEAEAEA 444
                       .||....|..    ::|..||:      ..|::.|.::     :....:::|.|
Human   321 -----------LSSTRGTGLP----AITDVPRR------ISDDDTATSDFCLWPSTLSKKSQAGA 364

  Fly   445 EEAEVGGLSRNGGGIQGPLHSESSPGSGGAFTALIQRSGKNPTELFGGFAAAGGNHFAPSGHSFN 509
            .|.             ||.   |.|....:.::|.:....||.           .|: |:..::.
Human   365 SEL-------------GPF---SDPRQFPSISSLTESRFSNPR-----------MHY-PATFTYT 401

  Fly   510 PALAAQLFL-----------QSPLLPQSSQWLYTQLYGSY--SDLPWLRNAAAAAAANINPGQEN 561
            |.:.:.:.|           ..|..|.|||    ...|.:  |..|:|....::.:...
Human   402 PPVTSGMSLGMSATTHYHTYLPPPYPGSSQ----SQSGPFQTSSTPYLYYGTSSGSYQF------ 456

  Fly   562 SGIPPLGSNPDHDGVNLIKRCVTLITHNPPDAENANPNASPPVSSTRRSPSPVETIDLDDVSTTS 626
                |:....|.....::..|.|  |.|  .:...|||.          |:..:.:|.|...::|
Human   457 ----PMVPGGDRSPSRMLPPCTT--TSN--GSTLLNPNL----------PNQNDGVDADGSHSSS 503

  Fly   627 RSASGSSGHGGGVGGGGAVGPIRTRTPKPSADVWRPY 663
            .:...|||                   :....|||||
Human   504 PTVLNSSG-------------------RMDESVWRPY 521

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RunxB	NP_001259745.1	Runt	115..239	CDD:459963	92/123 (75%)
RUNX2	NP_001019801.3	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	18..59		9/18 (50%)
		polyglutamine repeat	49..71		15/21 (71%)
		polyalanine repeat	73..89		2/15 (13%)
		Runt	102..230	CDD:459963	92/130 (71%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	222..340		42/203 (21%)
		Required for interaction with FOXO1. /evidence=ECO:0000250	242..258		3/15 (20%)
		Herpes_BLLF1	<266..481	CDD:282904	57/343 (17%)
		Interaction with KAT6A. /evidence=ECO:0000250	336..439		24/140 (17%)
		Interaction with KAT6B. /evidence=ECO:0000269|PubMed:11965546	374..468		18/119 (15%)
		RunxI	430..521	CDD:430037	25/137 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	460..521		18/93 (19%)