Protein Alignment HisRS and gcn2

DIOPT Version :9

• Sequence 1: NP_573305.2
• Gene: HisRS / 32841
• FlyBaseID: FBgn0027087
• Length: 564
• Species: Drosophila melanogaster
• Sequence 2: NP_595991.2
• Gene: gcn2 / 2540500
• PomBaseID: SPBC36B7.09
• Length: 1576
• Species: Schizosaccharomyces pombe
• Alignment Length: 566
• Identity: 129/566 - (22%)
• Similarity: 224/566 - (39%)
• Gaps: 92/566 - (16%)

  Fly    52 QQQPQKQQQAQVQQQQHEQLHQIDEEV----ARLLA----------LKATLGGDAAPTNQK---- 98
            |..|.|:..:|...:......::.||.    .|||:          ||...|  ..|...|
pombe   901 QHDPTKRPSSQELLESEAIPPKVGEEFIQEGLRLLSNPNTPYYLKLLKVLFG--QVPDRHKDFTY 963

  Fly    99 -FTLKTPKG----TRDYGPQQMTLRQGVLDKIVQVFKRHGGEAIDTPVFELKEVLTGKYGEDSKL 158
             |.|....|    ..|.|...: |...|.|.:|:||:|||.:..::.:...|   :.:|.:|...
pombe   964 DFNLSEESGVLSKVSDRGWDSL-LACLVRDHVVKVFRRHGAKERESHILFPK---SSQYDKDQAS 1024

  Fly   159 IYDLKDQGGEILSMRYDLTVPLARYLAMNKISSIKRYHIAKVYRRDNPAMTKGRYREFYQCDFDI 223
            : .|.|:.|.:|.:.||..:|.||.:|.|.:...|.|.|:.|:|.   |...||.:...:..|||
pombe  1025 V-SLLDKNGTLLQLPYDTVLPYARNVARNAVEEEKTYLISDVFRE---AKGGGRPKAIKEISFDI 1085

  Fly   224 AGTYDPM-LPDAECVKIVSEILDTL-DIGDYVIKLNHRQLLDGMFQACGVPADSFRTICSAVDKL 286
            ....|.: ..|||.:|.:.|:|..: .:.:..|.:||..:|..:.....|..|..|.....:.::
pombe  1086 TTNSDNLDWYDAETIKALDEVLTEIPSLTESCILINHADILSSILDYLQVSKDKRRMATHILGQI 1150

  Fly   287 D-KSPWADVRKEMVDEKGLDEAAADRIGEY---VRLSGGAELVEQLLANEKLKAVPNAVKGLEGM 347
            : :...:.||.::..|..:.....|.:..:   .....||..:.::...|..:.:..|:..:|.:
pombe  1151 NQRLTLSQVRNQLRIESLVPSTTLDDLSLFDFRENYEEGASKLRKIFGKEMPQKMRTALNYMERV 1215

  Fly   348 KQLLKYCSIFGLDKRVSFDLSLARGLDYYTGVIYEGVLKGESATVASPAKTSQQNGEQANEPATV 412
            .:||:..       ::|..|.. ..|..|....|:|.|..::..:|..::.
pombe  1216 VKLLRAL-------KISHQLYF-MPLCVYNFEFYDGGLMFQAINLAEKSEL-------------- 1258

  Fly   413 GSVAGGGRYDNLVGMFDP------RGKAVPCVGVSIGVER-IFSVL-----------EARAAASG 459
              :..|||||.||..|||      |.|.|  ||:...:|: :||:|           ...:.:..
pombe  1259 --ICAGGRYDKLVRFFDPPLMRTARKKHV--VGICFALEKLVFSMLRYIRFHNSKQSSKHSPSPT 1319

  Fly   460 LK----LRTSDVEVYVASAHKGLHEQRLKVLNLLWDAGVKAEHSYKLNPKLLVQLQHCEEHQIPL 520
            ||    .....|:|.|.|..|....::..:|..||...::|:...:....|...:.|.....|..
pombe  1320 LKSVGPWAPRRVDVLVTSIGKDSILEKCSLLQELWALNIQADIVLRGASSLEEIVTHYRSEGINW 1384

  Fly   521 VVVLGDAEL-AQGLVKLREVTTREETNVKLED----LAAEIRRRQQ 561
            |:|:..... .:..||.|.:...|:..::.::    |..||..|::
pombe  1385 VLVVRQKNTQMEHSVKARNILKNEDDEIRFDEVGMWLLGEINERKR 1430

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HisRS	NP_573305.2	S15_NS1_EPRS_RNA-bind	6..92	CDD:294248	12/53 (23%)
		HisS	98..561	CDD:223202	116/504 (23%)
		HisRS-like_core	114..452	CDD:238396	86/361 (24%)
		HisRS_anticodon	467..557	CDD:238436	20/94 (21%)
gcn2	NP_595991.2	RWD	10..124	CDD:283440
		PK_eIF2AK_GCN2_rpt1	241..508	CDD:270914
		STKc_EIF2AK4_GCN2_rpt2	549..917	CDD:270948	4/15 (27%)
		S_TKc	556..915	CDD:214567	4/13 (31%)
		HisS	990..1417	CDD:223202	105/459 (23%)
		class_II_aaRS-like_core	990..1295	CDD:294192	82/337 (24%)
		HGTP_anticodon2	1326..1576	CDD:289514	22/105 (21%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0124
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810