Protein Alignment HisRS and Hars

DIOPT Version :8

• Sequence 1: NP_573305.2
• Gene: HisRS / 32841
• FlyBaseID: FBgn0027087
• Length: 564
• Species: Drosophila melanogaster
• Sequence 2: NP_032240.3
• Gene: Hars / 15115
• MGIID: 108087
• Length: 509
• Species: Mus musculus
• Alignment Length: 559
• Identity: 329/560 (59%)
• Similarity: 397/560 (71%)
• Gaps: 58/560 (10%)

  Fly     1 MSDTREQILEQIKVQGDLVRQLKAAKESKEKTAPSGGGRVSSSGVPAQRQQQQQPQKQQQAQVQQ 65
            |:| |..:.|.:::||..||.||..|.|.|
Mouse     1 MAD-RAALEELVRLQGAHVRGLKEQKASAE----------------------------------- 29

  Fly    66 QQHEQLHQIDEEVARLLALKATLGGDAAPTNQKFTLKTPKGTRDYGPQQMTLRQGVLDKIVQVFK 130
                   ||:|||.:||.|||.||.|..  .|||.||||||||||.|:||.:|:.|.|.|::.||
Mouse    30 -------QIEEEVTKLLKLKAQLGQDEG--KQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFK 85

  Fly   131 RHGGEAIDTPVFELKEVLTGKYGEDSKLIYDLKDQGGEILSMRYDLTVPLARYLAMNKISSIKRY 195
            |||.|.||||||||||.|||||||||||||||||||||:||:|||||||.||||||||:::||||
Mouse    86 RHGAEVIDTPVFELKETLTGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRY 150

  Fly   196 HIAKVYRRDNPAMTKGRYREFYQCDFDIAGTYDPMLPDAECVKIVSEILDTLDIGDYVIKLNHRQ 260
            ||||||||||||||:|||||||||||||||.:|||:|||||:||:.|||.:|.||::::|:|.|:
Mouse   151 HIAKVYRRDNPAMTRGRYREFYQCDFDIAGQFDPMIPDAECLKIMCEILSSLQIGNFLVKVNDRR 215

  Fly   261 LLDGMFQACGVPADSFRTICSAVDKLDKSPWADVRKEMVDEKGLDEAAADRIGEYVRLSGGAELV 325
            :|||||..||||...||||||:||||||..|.:|:.|||.||||....|||||:||:..||..||
Mouse   216 ILDGMFAVCGVPDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLV 280

  Fly   326 EQLLANEKLKAVPNAVKGLEGMKQLLKYCSIFGLDKRVSFDLSLARGLDYYTGVIYEGVLKGESA 390
            ||||.:.||.....||:||..:|.|.:|..:||:|.::|||||||||||||||||||.||
Mouse   281 EQLLQDPKLSQNKQAVEGLGDLKLLFEYLILFGIDDKISFDLSLARGLDYYTGVIYEAVL----- 340

  Fly   391 TVASPAKTSQQNGEQANEPATVGSVAGGGRYDNLVGMFDPRGKAVPCVGVSIGVERIFSVLEARA 455
                 .:...|.||   ||..|||:|.|||||.|||||||:|:.|||||:|||||||||::|.|.
Mouse   341 -----LQMPTQAGE---EPLGVGSIAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRL 397

  Fly   456 AASGLKLRTSDVEVYVASAHKGLHEQRLKVLNLLWDAGVKAEHSYKLNPKLLVQLQHCEEHQIPL 520
            .||..|:||::.:|.||||.|.|.|:|||:::.|||||:|||..||.|||||.|||:|||..|||
Mouse   398 EASEEKVRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPL 462

  Fly   521 VVVLGDAELAQGLVKLREVTTREETNVKLEDLAAEIRRR 559
            |.::|:.||..|::|||.|.:|||.:|:.|||..|||||
Mouse   463 VAIIGEQELKDGVIKLRSVASREEVDVRREDLVEEIRRR 501

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HisRS	NP_573305.2	S15_NS1_EPRS_RNA-bind	6..92	CDD:294248	23/86 (27%)
		HisS	98..561	CDD:223202	302/463 (65%)
		HisRS-like_core	114..452	CDD:238396	226/338 (67%)
		HisRS_anticodon	467..557	CDD:238436	53/90 (59%)
Hars	NP_032240.3	HisRS_RNA	5..49	CDD:238474	23/86 (27%)
		HisS	56..501	CDD:223202	299/458 (65%)
		HisRS-like_core	69..394	CDD:238396	226/338 (67%)
		L-histidine binding. {ECO:0000250|UniProtKB:P12081}	130..132		2/2 (100%)
		L-histidine binding. {ECO:0000250|UniProtKB:P12081}	330..331		1/1 (100%)
		HisRS_anticodon	408..499	CDD:238436	53/91 (58%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930				C175314415
eggNOG	1	0.900				E1_COG0124
Hieranoid	1	1.000
Homologene	1	1.000				H1592
Inparanoid	1	1.050	623	1.000	Inparanoid score	I863
Isobase	1	0.950		0	Normalized mean entropy	S444
OMA	1	1.010
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000				FOG0002150
OrthoInspector	1	1.000				T616172
orthoMCL	1	0.900				OOG5_126806
Panther	1	1.100			LDO	PTHR11476
Phylome	1	0.910		0.800	Consistency score
RoundUp	1	1.030		avgDist	Average_Evolutionary_Distance	R1442
TreeFam	1	0.960
	14	13.740