Protein Alignment HUWE1 and HECW2

DIOPT Version :10

• Sequence 1: NP_001285288.1
• Gene: HUWE1 / 32510
• FlyBaseID: FBgn0030674
• Length: 5151
• Species: Drosophila melanogaster
• Sequence 2: XP_006712709.1
• Gene: HECW2 / 57520
• HGNCID: 29853
• Length: 1579
• Species: Homo sapiens
• Alignment Length: 1767
• Identity: 387/1767 - (21%)
• Similarity: 623/1767 - (35%)
• Gaps: 552/1767 - (31%)

  Fly  3736 VLMQQRIEQQRQAAQTANPEDPVDTAAFFQNLPEN--LRQA------ILTDMEESQIASLPPELA 3792
            :.:::|..|.|   .|.:||: :.:.|...::|||  |::|      :.::...|..||:.....
Human    10 LFVRRRNPQMR---YTLSPEN-LQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTL 70

  Fly  3793 AEAQFLRRDWESRNGA---------RMDAHPPSNALSRFQTTLQSLDDTRWHSSIWYDASGN--A 3846
            .:||.|...|:.:...         .:|.:.|:|.   :.:..:.:..|:....:|....|.  .
Human    71 GQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANF---WDSKNRGVTGTQKGQIVWRIEPGPYFM 132

  Fly  3847 QPQ-------HH------QHTT--IVHQHPHAHHAPNMG--------------KPLALLMMED-- 3880
            :|:       :|      :.||  |..::|    |..||              :.|....:.|
Human   133 EPEIKICFKYYHGISGALRATTPCITVKNP----AVMMGAEGMEGGASGNLHSRKLVSFTLSDLR 193

  Fly  3881 -----ENILLDPDSLATLLLFLFVEDQKVNSMRLHRVIRNLCHH-EPTRDWIISALISIIQKTNE 3939
                 :.:..:||.    .|.:.::..|.:|      .....|| :..|..|||         |.
Human   194 AVGLKKGMFFNPDP----YLKMSIQPGKKSS------FPTCAHHGQERRSTIIS---------NT 239

  Fly  3940 DNANFGAGQAGGKPEW--------------LKLRVDAAFGYKSNI---FL------INRLYEGSA 3981
            .|           |.|              |::.:...|.....|   ||      :.||.|..|
Human   240 TN-----------PIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQA 293

  Fly  3982 RSISINPQAAQMIVRNCLDLLFVLAKHYP----SSFVPFNRDVKARRILSAVFGM--PSPTA-GS 4039
                |..|.          |.:.|.:..|    |.::.|..:|.:......:|..  .||.| |:
Human   294 ----IGDQM----------LSYNLGRRLPADHVSGYLQFKVEVTSSVHEEMLFSHSDASPEAVGT 344

  Fly  4040 GGGAGSGSAASGGLTGYDDVPPGGTLDFRSRFCRYQVVNRLRSIFDDHPA---PKPS-------- 4093
            ..|..|.:...|..:..:|: ||...|  |:.|....|:      :|..|   ||.|
Human   345 ILGVNSVNGDLGSPSDDEDM-PGSHHD--SQVCSNGPVS------EDSAADGTPKHSFRTSSTLE 400

  Fly  4094 VPLDEAPSTSKAA----------------------AAKAAATAKVSTAGSQNKHSVPYQTNAKNF 4136
            :..:|..|||...                      :....||....:.|:..|....:.|:.:
Human   401 IDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTR-- 463

  Fly  4137 WDVVLNIDQQTQ-----------------------VRLANVAQFPLTSQPAWEWQP------NTA 4172
            .:.:|:||...:                       .|.:......||||...|..|      :|.
Human   464 LNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASTH 528

  Fly  4173 DFLSFSESPFGQLLEMLPYKVICRSPHLTDMLVKLLASLSTELPKEEELLPLSDQMPPLVPIGPV 4237
            :..||.:.|     |.||                             ||...|      :|.||.
Human   529 EAASFEDKP-----ENLP-----------------------------ELAESS------LPAGPA 553

  Fly  4238 QRAGAAAAAAHPPPPQPPAENTATATEIGDGLAANIEEQASPASG-NPASGDSNAGAKRTQLPTL 4301
            ...|...     |.|||.|:.       |.......:|...|.|| :..:.|::.|::|....|.
Human   554 PEEGEGG-----PEPQPSADQ-------GSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETE 606

  Fly  4302 SVALPVTPSKPRRKIYSKNFSQLQLVIEVLTH-------QCGTTEGLDNVAKLIVNL-TQCSQAS 4358
            |:.....||:...:....:.::.:.|.|..|.       :|..:...::|...:.:: |:||...
Human   607 SLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVTTQLSSVDTRCSSLE 671

  Fly  4359 SAIFIQYLTAAILGLAEEVRQAIQMLLNEIRMYNIHQGGAGTTSQAASTSGAAAATASYVPTVSG 4423
            ||.|.:  |.|.....||                               .||.||.    ||.||
Human   672 SARFPE--TPAFSSQEEE-------------------------------DGACAAE----PTSSG 699

  Fly  4424 ASLLPNLAVHEGIMQDRFTAEHVIISAPKNAKPTCELQLPSMKKLMSNSSAQPYFLRTLKIFMQV 4488
            .:        ||..:...||.    |.|....|:.|.:.|..:.            .|:....::
Human   700 PA--------EGSQESVCTAG----SLPVVQVPSGEDEGPGAES------------ATVPDQEEL 740

  Fly  4489 RDMYEAQNGLSTGGGVACGAGDNDDDIIDIEG--------DGGASGGSSSD-------------- 4531
            .::::.:..|......|......:....:.:|        :.||:|||.::
Human   741 GEVWQRRGSLEGAAAAAESPPQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRSLPSVRQD 805

  Fly  4532 ----------MAPGSASTI----------------------SPPPP---METNQIAQDGD----- 4556
                      :.|...:.|                      :||.|   ..:|.|.|...
Human   806 VSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRY 870

  Fly  4557 -------DDEEDDDEARSFPGVGNHA---VASASKSTKPTLSQILCLDVLWNTLSECLVALEESK 4611
                   .:|..::...:..|.|..|   .|||....:..|........:...|....|....|.
Human   871 QSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRITLLLQSPPVKFLISP 935

  Fly  4612 DEFAVLVLQPTVEAFF-----LIHA-------SH--------------------------RGASK 4638
            :.|.||...|:....|     |.|.       :|                          ||...
Human   936 EFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEM 1000

  Fly  4639 K---SGRSFM---------------------PNAA-----GFGLRAAAGAGAIGGLGAAAPSAQE 4674
            |   .|::|.                     |.:|     ....:.:..||.:|     ..|...
Human  1001 KHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVG-----EDSRHA 1060

  Fly  4675 YSPFMDDVSSA-NTPSSASFDDEPRMQDASTNTPLVT---NPSTSTAMSAHEAQLRQD---RQKF 4732
            ..|.:...||. ||.|...:.|   |...:.|..:|.   .|:....:...:..|.::   |:|
Human  1061 GPPVLPRPSSTFNTVSRPQYQD---MVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREK- 1121

  Fly  4733 LQFAEKHRT------------VL------NQILRQSPTHL------------------------- 4754
            :||.....|            |:      .:|:...|.|.
Human  1122 IQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQ 1186

  Fly  4755 -----SDGPFAVLVDHTRILDFDVKRKYFQTELERLDEGIRREEHTVSVRRVTVFEDSFRVLYRL 4814
                 :..|:.        .||:.|.:.|..:||....|....:..:.:||..:.||:|..:...
Human  1187 RANARAPAPYK--------RDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGY 1243

  Fly  4815 GPEE-WKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANP 4878
            ..:: .:|:.|:.|..|||.|..|..||::.::|||:|||.|.||..|..|..|..|:|.|....
Human  1244 SRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVD 1308

  Fly  4879 NHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDIS 4943
            ||..:|:|.||::..|:....||:.:|||.|||.:|......:|:|..|.||::.|.::..|||.
Human  1309 NHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIH 1373

  Fly  4944 TLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFY 5008
            .: .:|||:...:.||....|:|||.|.:..|||:||.||::.:.:.::...:.||.::.:.|||
Human  1374 DI-LDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFY 1437

  Fly  5009 DIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYH-KYTSKSAQIQWFWRALRSFDQADRAK 5072
            :::...|:|:|:.:||||:|:|..:||:.|.:.||||. .|......|:|||.|:..|:...|.:
Human  1438 EVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLR 1502

  Fly  5073 FLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCL 5137
            .||||||||.:|.:||.||.|.||.::|.:.:..:.| .||.||||||:||||.|.|:..|...|
Human  1503 LLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKIT-ALPRAHTCFNRLDLPPYPSFSMLYEKL 1566

  Fly  5138 LKAIHECSEGFG 5149
            |.|:.|.|. ||
Human  1567 LTAVEETST-FG 1577

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:428721
		DUF913	621..989	CDD:461803
		UBA_like_SF	1467..1506	CDD:473871
		WWE	1756..1828	CDD:128922
		DUF5585	3288..3672	CDD:465521
		UBM	3670..3700	CDD:464159
		UBM	3719..3746	CDD:464159	2/9 (22%)
		HECTc	4796..5149	CDD:238033	139/354 (39%)
HECW2	XP_006712709.1	HECW_N	45..162	CDD:465177	19/119 (16%)
		C2_NEDL1-like	185..321	CDD:176073	32/179 (18%)
		DMP1	<352..707	CDD:462128	88/462 (19%)
		PHA03169	591..>801	CDD:223003	48/270 (18%)
		WW	816..845	CDD:459800	2/28 (7%)
		HECW1_helix	922..988	CDD:465766	11/65 (17%)
		WW	994..1023	CDD:459800	5/28 (18%)
		HECTc	1223..1577	CDD:238033	139/356 (39%)