Protein Alignment HUWE1 and Hecw

DIOPT Version :10

• Sequence 1: NP_001285288.1
• Gene: HUWE1 / 32510
• FlyBaseID: FBgn0030674
• Length: 5151
• Species: Drosophila melanogaster
• Sequence 2: NP_572574.2
• Gene: Hecw / 31905
• FlyBaseID: FBgn0261931
• Length: 1426
• Species: Drosophila melanogaster
• Alignment Length: 1822
• Identity: 400/1822 - (21%)
• Similarity: 611/1822 - (33%)
• Gaps: 622/1822 - (34%)

  Fly  3491 AALEQLNVTAPDVVRTDRNFALPSSLEVDVDVL------PGIVDLPQQEAQPEDDQLLLPPPPPP 3549
            |..|..|....|.:......||..:.:|.|.|.      |.:....|:.|:.|:.|..|.....|
  Fly    62 AFYEARNGNESDELEQAFQSALTLTNDVHVSVSEVEEHEPPLASRNQEVAEVEEQQQQLGACALP 126

  Fly  3550 PPSDTTPLVHAEDDLWPGTVVSAGTRE-ESSAPAVATESTEAVNESNQPEPTPESSESTSP---- 3609
            .||:.:            ...|..||| ......::|:|.|          ||.::.:.||
  Fly   127 QPSEAS------------LTGSKETREPRHHQTLLSTKSCE----------TPANNATLSPTNSY 169

  Fly  3610 -----------------------NPQAPSAEPTPLEPGAAVDASVGAQTPATTEEAASTAGASGT 3651
                                   :||  |...|||. ||......||  |.||   .|.|...|.
  Fly   170 NGSIGSDGGSSVGGGARRKSWTLSPQ--SGGSTPLS-GAKKKTKTGA--PTTT---LSNANGDGF 226

  Fly  3652 TATITDMSPEVRAALGDLEVP-EGVDPSFLAALPSEMREEVIQEHLRMQRIRQRAQQNAIQIAHD 3715
            ...:.....|.        || |.||...:|.:..|:.||..:|                ..|.:
  Fly   227 NLWVARECFET--------VPAEMVDTLSMAEVDEEVDEEEDEE----------------AAAVE 267

  Fly  3716 SLVEVNPEFLAALPLNIQSEVLMQQRIEQQRQAAQTANPEDPVDTAAFFQNLPENLRQAILTDME 3780
            ||:.      ||..|...:.|   ||.:||:.......|..|       |:.|
  Fly   268 SLLG------AAAALQASAHV---QRRQQQQHHQHLRQPSPP-------QHRP------------ 304

  Fly  3781 ESQIASLPPELAAEAQFLRRDWESRNGARMDAHPPSNALSR--------FQTTLQSLDDTRWHSS 3837
                             |.|.|            |..|:.|        ||..:...|:......
  Fly   305 -----------------LERSW------------PPRAVGRDLKLQGDVFQFDILDTDERLAEEM 340

  Fly  3838 IWYDASGNAQPQHHQHTTIVHQHPHAHHAPNMGKPLALLMMEDENILLDPDSLATLLLFLFVEDQ 3902
            .....|.|..|.         ..|.....|.:.|.:.            |||.
  Fly   341 AQGSGSSNNSPL---------ATPPVRFKPLLKKKIG------------PDSY------------ 372

  Fly  3903 KVNSMRLHRVIRNLCHHEPTRDWIISALISIIQKTNEDNANFGAGQAGGKPEWLKLRVDAAFGY- 3966
             :|::...:|..:..:..||..                 ::...|.:...|..|:      |.|
  Fly   373 -INTISTQKVPVHQSYEFPTFP-----------------SSSAVGSSSSSPNSLQ------FPYL 413

  Fly  3967 KSNIFLINRLYEGSARS--------ISINPQAAQMIVRNC-------LDLLFVLAKHYPS----- 4011
            :.:..|...|..|.|.|        :.::|:..:.....|       ||:.....:|.|:
  Fly   414 RQHRPLTRALSNGKASSSEEQASPRLLLSPKRPRSPPSGCSTRSASPLDISLSPEQHSPTRGRGV 478

  Fly  4012 -----SFVP-----FNRDVKARRILSAVFGMPSPTAGSGGGAGSGSAASGGLTGYDDVPPGGTLD 4066
                 ..||     |:.....:|:|..|          |||||:|  |:||.||...|.|.....
  Fly   479 GGALQDAVPPGTPTFHGQRPQQRLLKRV----------GGGAGAG--ATGGATGSPLVCPPTPTH 531

  Fly  4067 FRSRFCRYQVVNRLRSIFDDHPAPKPSVPLDEAPSTSKAAAAKAAATAKVSTAGSQNKHSVPYQT 4131
            ...|..|.|.|....|:     |...::|.| .|:.|.                     .:.|
  Fly   532 HARRHARLQAVTANSSL-----ASGGAIPSD-PPAVSS---------------------QLDY-- 567

  Fly  4132 NAKNFWDVVLNIDQQTQVRLANVAQFPLTSQPAWEWQPNTADFLSFSESPFGQLLEMLPYKVICR 4196
                   :.|....|.|                                  ||
  Fly   568 -------IYLQDGGQEQ----------------------------------GQ------------ 579

  Fly  4197 SPHLTDMLVKLLASLSTELPKEEELLPLSDQMPPLVPIGPVQRAGAAAAAAHPPPPQPPAENTAT 4261
                               .:.:|::.:|....|.:|    :|:.|||||             |.
  Fly   580 -------------------EQSDEVVHISSTRLPSIP----ERSAAAAAA-------------AA 608

  Fly  4262 ATEIGDGLAANIEEQASPASGNPASGDSNAGAKRTQLPTLSVALPVTPS-KPRRKIYSKNFSQLQ 4325
            |...|   ||.:|    |.:|. .||.|.|||....        |:.|: :.|...:.:.|
  Fly   609 AAAAG---AALME----PGTGT-VSGSSGAGAAGEP--------PLPPAWEARMDSHGRIF---- 653

  Fly  4326 LVIEVLTHQCGTTEGLDNVAKLIVNLTQCSQASSAIFIQYLTAAILGLAEEVRQAI--------Q 4382
                .:.|...||..               |...|.    .:....|..:..||.:        :
  Fly   654 ----YIDHTTRTTSW---------------QRPGAA----PSGGPAGREQHHRQQLDRRYQSIRR 695

  Fly  4383 MLLNEIRMYNIHQGGAGTTSQAASTSGAAAATASYV-PTV------SGASLLPNLAVHEGIMQDR 4440
            .:.||.|...::.......|.|.||:.||...|:.: |.:      ...|||........|....
  Fly   696 TITNESRAAQLYNLPTSANSNANSTAPAAPPPATAIHPAILMLCRPDFYSLLHTNEAALAIYNRN 760

  Fly  4441 FTAEHVIISAPKNAKPTC--------------------ELQLPSMKKLMSNSSAQPYFL-----R 4480
            ...:|:::...::  |.|                    ..:|||..:...:.|.:.:|:     |
  Fly   761 AALKHMVMRIRRD--PPCFQRYQYNKDLVALVNTFAQMSRELPSCWETKLDQSGKQFFIDHQHRR 823

  Fly  4481 TLKIFM------------QVRDMYEAQNGLSTGGGVACGAGDNDDDIIDIEGDGGASGGSSSDMA 4533
            |  .||            :.|..:..|..         ..|....|::    ||.....:|..:.
  Fly   824 T--SFMDPRLPVECPRVVRHRQQHSQQQS---------SVGYYYPDLV----DGNCLSATSGHIN 873

  Fly  4534 PGSASTISPPPPMETNQIAQDGDDDEEDDDEARSFPGVGNHAVASASKSTKPTLSQILCLDVLWN 4598
            ...||::|..||:...:       .......:||..|.|::.                      |
  Fly   874 ASPASSVSGVPPLPPPR-------PSSSSSSSRSSHGHGHNC----------------------N 909

  Fly  4599 TLSECLVALEESKDEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGAIG 4663
            |.|....||...:..:                                .|||.   |:|||.|.|
  Fly   910 TTSAISCALNADRSAY--------------------------------GAAGM---ASAGATAAG 939

  Fly  4664 GLGAAAPSAQEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMS--------- 4719
                           .:||..|......:|..:|.:.:      ::........||
  Fly   940 ---------------YEDVPIAYNEKVIAFLRQPNILE------ILRERQGQHTMSRQLREKINI 983

  Fly  4720 ------------AHEAQL-------RQDRQKFLQFAEKH---RTVLNQILRQSPTHLSDGPFAVL 4762
                        .|:.||       .|:...|:...|:.   ..|||..:.|.    :..||.
  Fly   984 LRVEGVPALERLGHDLQLTILLSLFEQEIMGFVPIEERSPQGSPVLNSRMPQR----APPPFR-- 1042

  Fly  4763 VDHTRILDFDVKRKYFQTELERLDEGIRREEHTVSVRRVTVFEDSFRVLYRLGPEE-WKNRFYIV 4826
                  .||:.|.:.|..:||....|....:..:.:||..:.||:||.:.....:: .:.|..::
  Fly  1043 ------RDFEAKLRSFYRKLESKGYGQGPHKLKLHIRRSHLLEDAFRRIMSANKKDLQRGRLAVL 1101

  Fly  4827 FEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVI 4891
            ::.|||.|.||..||::.::|||:|||.|.||..|..|..|..::|.|....|...:|:|.|||:
  Fly  1102 WDTEEGLDYGGPSREFFFLLSRELFNPYYGLFEYSANDTYTVQVSPLSAFVDNCHDWFRFSGRVL 1166

  Fly  4892 AKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDIST---LGYELTFST 4953
            ..|:....||:.:|||.|||.:|...|..:|:||.|.||::.|.::..|||.|   ||  |||..
  Fly  1167 GLALVHQYLLDAFFTRPFYKALLRLPVALSDLESLDNEFHQSLQWIRDNDIGTGVDLG--LTFCV 1229

  Fly  4954 EVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISI 5018
            ..:..|....|:|||.|::..:.|:||.||::.:.:.::...:::|.::.:.|||::|...|:|:
  Fly  1230 TEELLGSVVDRELKPGGKNIIINEKNKKEYLERMIKWRLERGVQEQTESLVRGFYEVIDSRLVSV 1294

  Fly  5019 FNEQELELLISGLPDIDIEDLKANTEYHK-YTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSK 5082
            |:.:||||:|:|..:||..|.:.||||.. |......|.|||:.:..|....|.:.||||||||.
  Fly  1295 FDARELELVIAGTAEIDTNDWRLNTEYRSGYHDNHQVIVWFWQVIERFSNEQRLRLLQFVTGTSS 1359

  Fly  5083 VPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEG 5147
            :|.:||.:|.|..|.::|.|.:..: .:.||.||||||:||||.|.:.:.|...||.|:.| :..
  Fly  1360 IPYEGFSALRGSTGPRRFCIEKWGK-PNALPRAHTCFNRLDLPPYPTPELLYEKLLLAVEE-TNT 1422

  Fly  5148 FG 5149
            ||
  Fly  1423 FG 1424

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:428721
		DUF913	621..989	CDD:461803
		UBA_like_SF	1467..1506	CDD:473871
		WWE	1756..1828	CDD:128922
		DUF5585	3288..3672	CDD:465521	46/214 (21%)
		UBM	3670..3700	CDD:464159	10/30 (33%)
		UBM	3719..3746	CDD:464159	7/26 (27%)
		HECTc	4796..5149	CDD:238033	135/357 (38%)
Hecw	NP_572574.2	PHA03247	<411..562	CDD:223021	40/168 (24%)
		WW	638..667	CDD:459800	7/51 (14%)
		HECW1_helix	738..794	CDD:465766	7/57 (12%)
		HECTc	1068..1424	CDD:238033	135/359 (38%)