Protein Alignment HUWE1 and Hecw2

DIOPT Version :10

• Sequence 1: NP_001285288.1
• Gene: HUWE1 / 32510
• FlyBaseID: FBgn0030674
• Length: 5151
• Species: Drosophila melanogaster
• Sequence 2: NP_001101688.1
• Gene: Hecw2 / 316395
• RGDID: 1593244
• Length: 1578
• Species: Rattus norvegicus
• Alignment Length: 1806
• Identity: 401/1806 - (22%)
• Similarity: 636/1806 - (35%)
• Gaps: 584/1806 - (32%)

  Fly  3693 QEHLRMQRIRQRAQQNAIQIAHDSLVEVNPEFLAAL-PLNIQSEVLMQQRIEQQRQAAQTANPED 3756
            :|||...|.|                  ||:....| |.|:||  |..|....:..|.|.||.:.
  Rat     6 REHLLFVRRR------------------NPQMRYTLSPENLQS--LATQNSMPENMALQRANSDT 50

  Fly  3757 PVDTAAFFQNLPENLRQAILTDMEESQIASLPPELAAEAQFLRRDWESRNGA---------RMDA 3812
            .:                :.::...|..||:......:||.|...|:.:...         .:|.
  Rat    51 DL----------------VTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDE 99

  Fly  3813 HPPSNALSRFQTTLQSLDDTRWHSSIWYDASGN--AQPQ-------HH------QHTT--IVHQH 3860
            :.|:|.   :.:..:.:..|:....:|....|.  .:|:       :|      :.||  |..::
  Rat   100 NSPANF---WDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKN 161

  Fly  3861 PHAHHAPNMG--------------KPLALLMMED-------ENILLDPDSLATLLLFLFVEDQKV 3904
            |    |..||              :.|....:.|       :.:..:||.    .|.:.::..|.
  Rat   162 P----AVMMGAEGMEGCASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDP----YLKMSIQPGKK 218

  Fly  3905 NSMRLHRVIRNLCHH-EPTRDWIISALISIIQKTNEDNANFGAGQAGGKPEW------------- 3955
            :|      .....|| :..|..|||         |..|           |.|
  Rat   219 SS------FPTCAHHGQERRSTIIS---------NTTN-----------PIWHREKYSFFALLTD 257

  Fly  3956 -LKLRVDAAFGYKSNI---FL------INRLYEGSARSISINPQAAQMIVRNCLDLLFVLAKHYP 4010
             |::.:...|.....|   ||      :.||.|..|       ...||:..|       |.:..|
  Rat   258 VLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQA-------VGDQMLSYN-------LGRRLP 308

  Fly  4011 ----SSFVPFNRDVKARRILSAVFGMPSPTA-GS-------GGGAGSGSAASGGLTGYDDVPPGG 4063
                |.::.|..:|.:.....|     ||.| |:       .|..||.|.        |:..||.
  Rat   309 ADHVSGYLQFRVEVPSSAQEDA-----SPEAVGTILGVHTVNGDLGSPSD--------DEDMPGS 360

  Fly  4064 TLDFRSRFCRYQVVNRLRSIFDDHPAPKPSVPLDEAPSTSKAAAAKAAATAKVST---AGSQNKH 4125
            ..|  |..|.            :.|..:.|| .|..|..|    .:.::|.::.|   ..:.:::
  Rat   361 HHD--STICA------------NGPVSEDSV-ADGTPKHS----FRTSSTLEIDTEDLISTSSRN 406

  Fly  4126 SVP--YQTNAKNFWDVVLNIDQQTQVRLANVAQFPLTSQPAWEWQPNTADFLSFSESPFGQLLEM 4188
            |.|  .|.:..::.|.:.:                  :.|:   :|.||   |.||.|.|...::
  Rat   407 SPPRGRQDSLNDYLDAIEH------------------NGPS---RPGTA---SSSERPMGASPKL 447

  Fly  4189 LPYKVICRSPHLTDMLVKLLASLSTE---------------LPKEEELL-----------PLSDQ 4227
                   ||...||..:..:..:.::               |.:|..|:           .|:.|
  Rat   448 -------RSSFPTDTRLNAMLHIDSDEEDHDFQQDLGYPSSLEEEGGLIMCSRTSRIDDGSLTSQ 505

  Fly  4228 MPPLVPIGPVQRAGAA----AAAAHPPPPQPPAEN----TATATEIGDGLAANIEEQAS------ 4278
            ..| ....||:...|:    |:....|...|..|:    :.||.:..:   |::|.|.|
  Rat   506 TKP-EDDNPVENEDASVHETASLEERPENLPEVEDGSLPSGTAPDENE---ASVEPQPSADQGST 566

  Fly  4279 ----------PASGNPA-SGDSNAGAKRTQLPTLSVALPVTPSKPRRKIYSKNFSQLQLVIEVLT 4332
                      |.||..| :.|::.|::|....|.|:.....||:...:....:.::.:.|.|..|
  Rat   567 ELCSSQEVDQPTSGTDAGASDTSVGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEAST 631

  Fly  4333 HQCGTT--EGLDNVAKLIVNL------TQCSQASSAIFIQYLTAAILGLAEEVRQAIQMLLNEIR 4389
            ...|.:  ||.|:.....|..      |:||...||.|.:  |.|.....||
  Rat   632 RPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPE--TPAFSSQEEE------------- 681

  Fly  4390 MYNIHQGGAGTTSQAASTSGAAAATASYVPTVSGASLLPNLAVHEGIMQDRFTAEHVIISAPKNA 4454
                              .||.||.    ||.||.:        ||..:...|..    |.|
  Rat   682 ------------------DGACAAE----PTSSGPT--------EGSQESVCTPG----SLP--- 709

  Fly  4455 KPTCELQLPSMKKLMSNSSAQPYFLRTLKIFMQVRDMYEAQNGLSTGGGVACGAG-----DNDDD 4514
                .:|:||.::..|.:.|     ..|....::.::::.:..|......|..|.     ..|.|
  Rat   710 ----AVQVPSREEEGSGAEA-----TALSEQGELGEVWQRRGSLEGAAAAATAAATDSQPQEDGD 765

  Fly  4515 IIDIEG--------DGGASGGSSSD------------------------MAPGSASTI------- 4540
            ..|.:|        :.||:|||.::                        :.|...:.|
  Rat   766 PGDTQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIF 830

  Fly  4541 ---------------SPPPP---METNQIAQDGD------------DDEEDDDEARSFPGVGNHA 4575
                           :||.|   ..:|.|.|...            .:|..::...:..|.|..|
  Rat   831 YVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEA 895

  Fly  4576 ---VASASKSTKPTLSQILCLDVLWNTLSECLVALEESKDEFAVLVLQPTVEAFF-----LIHA- 4631
               .|||....:..|........|...|....|....|.:.|.||...|:....|     |.|.
  Rat   896 DFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMI 960

  Fly  4632 ------SH--------------------------RGASKK---SGRSFM---------------- 4645
                  :|                          ||...|   .|::|.
  Rat   961 TKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLP 1025

  Fly  4646 -----PNAA-----GFGLRAAAGAGAIGGLGAAAPSAQEYSPFMD-DVSSANTPSSASFDDEPRM 4699
                 |.:|     ....:.:..||.:|     ..|.....|.:. ..|:.||.|...:.|   |
  Rat  1026 LQSSRPTSALVHRQHLTRQRSHSAGEVG-----EDSRHAGPPVLPRPASTFNTVSRPQYQD---M 1082

  Fly  4700 QDASTNTPLVT---NPSTSTAMSAHEAQLRQD---RQKFLQFAEKHRT------------VL--- 4743
            ...:.|..:|.   .|:....:...:..|.::   |:| :||.....|            |:
  Rat  1083 VPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREK-IQFIRTEGTPGLVRLSSDADLVMLLS 1146

  Fly  4744 ---NQILRQSPTHL------------------------------SDGPFAVLVDHTRILDFDVKR 4775
               .:|:...|.|.                              :..|:.        .||:.|.
  Rat  1147 LFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYK--------RDFEAKL 1203

  Fly  4776 KYFQTELERLDEGIRREEHTVSVRRVTVFEDSFRVLYRLGPEE-WKNRFYIVFEDEEGQDAGGLL 4839
            :.|..:||....|....:..:.:||..:.||:|..:.....:: .:|:.|:.|..|||.|..|..
  Rat  1204 RNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPS 1268

  Fly  4840 REWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECY 4904
            ||::.::|||:|||.|.||..|..|..|..|:|.|....||..:|:|.||::..|:....||:.:
  Rat  1269 REFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAF 1333

  Fly  4905 FTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPN 4969
            |||.|||.:|......:|:|..|.||::.|.::..|||..: .:|||:...:.||....|:|||.
  Rat  1334 FTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDI-LDLTFTVNEEVFGQITERELKPG 1397

  Fly  4970 GRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDI 5034
            |.:..|||:||.||::.:.:.::...:.||.::.:.|||:::...|:|:|:.:||||:|:|..:|
  Rat  1398 GANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEI 1462

  Fly  5035 DIEDLKANTEYH-KYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQ 5098
            |:.|.:.||||. .|......|:|||.|:..|:...|.:.||||||||.:|.:||.||.|.||.:
  Rat  1463 DLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPR 1527

  Fly  5099 KFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGFG 5149
            :|.:.:..:.| .||.||||||:||||.|.|:..|...||.|:.|.|. ||
  Rat  1528 RFCVEKWGKIT-ALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETST-FG 1576

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:428721
		DUF913	621..989	CDD:461803
		UBA_like_SF	1467..1506	CDD:473871
		WWE	1756..1828	CDD:128922
		DUF5585	3288..3672	CDD:465521
		UBM	3670..3700	CDD:464159	3/6 (50%)
		UBM	3719..3746	CDD:464159	9/27 (33%)
		HECTc	4796..5149	CDD:238033	139/354 (39%)
Hecw2	NP_001101688.1	HECW_N	45..162	CDD:465177	20/135 (15%)
		C2_NEDL1-like	185..321	CDD:176073	32/179 (18%)
		DMP1	<345..731	CDD:462128	102/508 (20%)
		NESP55	<464..632	CDD:115071	32/171 (19%)
		WW	815..844	CDD:459800	2/28 (7%)
		HECW1_helix	921..987	CDD:465766	11/65 (17%)
		WW	993..1022	CDD:459800	5/28 (18%)
		HECTc	1222..1576	CDD:238033	139/356 (39%)